A structural basis for the regulation of an H-NOX-associated cyclic-di-GMP synthase/phosphodiesterase enzyme by nitric oxide-bound H-NOX.

abstract：:To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate in comparison to MES- and HEPES-buffered references on the chemical shift perturbation an...

journal_title：Biochemistry

authors： Wong M,Khirich G,Loria JP

更新日期：2013-09-17 00:00:00

abstract：:Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However...

journal_title：Biochemistry

authors： Lamos ML,Turner DH

更新日期：1985-06-04 00:00:00

abstract：:Past evidence has indicated that Mycobacterium gordonae, as isolated from soil and as an occasional opportunistic pathogen, exists as a serocomplex. We now demonstrate that the basis of seroreactivity and diversity is a novel series of alkali-labile, trehalose-containing lipooligosaccharides (LOS). The structures from...

journal_title：Biochemistry

authors： Besra GS,McNeil MR,Khoo KH,Dell A,Morris HR,Brennan PJ

更新日期：1993-11-30 00:00:00

abstract：:Self-splicing of Tetrahymena pre-rRNA proceeds in two consecutive phosphoryl transesterification steps. One major difference between these steps is that in the first an exogenous guanosine (G) binds to the active site, while in the second the 3'-terminal G414 residue of the intron binds. The first step has been extens...

journal_title：Biochemistry

authors： Mei R,Herschlag D

更新日期：1996-05-07 00:00:00

abstract：:Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...

journal_title：Biochemistry

authors： Thielges MC,Chung JK,Axup JY,Fayer MD

更新日期：2011-06-28 00:00:00

abstract：:Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...

journal_title：Biochemistry

authors： Jelicks LA,Broido MS,Becker JM,Naider FR

更新日期：1989-05-16 00:00:00

abstract：:The understanding of interactions between small molecules and DNA is crucial to design new anticancer drugs targeted to DNA. Methylene blue (MB) is a phenothiazinium dye that has shown promising results in photodynamic therapy treatment. The noncovalent binding of methylene blue to DNA was experimentally and theoretic...

journal_title：Biochemistry

authors： Nogueira JJ,González L

更新日期：2014-04-15 00:00:00

abstract：:Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constr...

journal_title：Biochemistry

authors： Banci L,Bertini I,De la Rosa MA,Koulougliotis D,Navarro JA,Walter O

更新日期：1998-04-07 00:00:00

abstract：:We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK...

journal_title：Biochemistry

authors： Weeks AM,Coyle SM,Jinek M,Doudna JA,Chang MC

更新日期：2010-11-02 00:00:00

abstract：:Haloalkane dehalogenase converts haloalkanes to their corresponding alcohols and halides. The reaction mechanism involves the formation of a covalent alkyl-enzyme complex which is hydrolyzed by water. The active site is a hydrophobic cavity buried between the main domain and the cap domain of the enzyme. The enzyme ha...

journal_title：Biochemistry

authors： Schanstra JP,Janssen DB

更新日期：1996-05-07 00:00:00

abstract：:The slowly hydrolyzed ATP analog adenosine 5'-(gamma-thiotriphosphate) (ATP gamma S) has been used in many studies of the muscle motor protein myosin in order to form a stable "weak binding" state analogous to the actin-S1-ATP complex However, the results from studies using ATP gamma S do not always agree with the res...

journal_title：Biochemistry

authors： Resetar AM,Chalovich JM

更新日期：1995-12-12 00:00:00

abstract：:The cardiac and skeletal muscle sarcoplasmic reticulum ryanodine receptor Ca(2+) release channels contain thiols that are potential targets of endogenously produced reactive oxygen and nitrogen intermediates. Previously, we showed that the skeletal muscle ryanodine receptor (RyR1) has O(2)-sensitive thiols; only when ...

journal_title：Biochemistry

authors： Sun J,Yamaguchi N,Xu L,Eu JP,Stamler JS,Meissner G

更新日期：2008-12-30 00:00:00

abstract：:The structural behavior of the nucleosome core particles in the range of solvent Na+ concentration from 10.45 to 0.45 mM has been studied by small-angle neutron and synchroton radiation X-ray scattering, sedimentation, atomic absorption spectroscopy, density measurements, and circular dichroism. With decreasing salt c...

journal_title：Biochemistry

authors： Hirai M,Niimura N,Zama M,Mita K,Ichimura S,Tokunaga F,Ishikawa Y

更新日期：1988-10-04 00:00:00

abstract：:Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...

journal_title：Biochemistry

authors： Laberge M,Osvath S,Fidy J

更新日期：2001-08-07 00:00:00

abstract：:The RcnR metalloregulator represses the transcription of the Co(II) and Ni(II) exporter, RcnAB. Previous studies have shown that Co(II) and Ni(II) bind to RcnR in six-coordinate sites, resulting in derepression. Here, the roles of His60, His64, and His67 in specific metal recognition are examined. His60 and His64 corr...

journal_title：Biochemistry

authors： Higgins KA,Hu HQ,Chivers PT,Maroney MJ

更新日期：2013-01-08 00:00:00

abstract：:Peptidyl derivatives of diphenyl (alpha-aminoalkyl)phosphonates have been synthesized and are effective and specific inhibitors of serine proteases at low concentrations. Z-PheP(OPh)2 irreversibly reacts with chymotrypsin (kobsd/[I] = 1200 M-1 s-1) and does not react with two elastases. The best inhibitor for most chy...

journal_title：Biochemistry

authors： Oleksyszyn J,Powers JC

更新日期：1991-01-15 00:00:00

abstract：:The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistr...

journal_title：Biochemistry

authors： Hofmann K,Zhang WJ,Romovacek H,Finn FM,Bothner-By AA,Mishra PK

更新日期：1984-06-05 00:00:00

abstract：:The organization of the higher order structure of chromatin has been examined in chicken erythrocyte. Chromatin solubilized during the time course of a gentle micrococcal nuclease digestion of nuclei shows a continuous variation in the distribution of molecular weights. Electron microscopy studies of large chromatin f...

journal_title：Biochemistry

authors： Ruiz-Carrillo A,Puigdomènech P,Eder G,Lurz R

更新日期：1980-06-10 00:00:00

abstract：:Amphotericin B (AmB) is a polyene macrolide antibiotic isolated from Streptomyces nodosus. The antifungal activity of AmB can be attributed to the formation of an ion-channel assembly in the presence of ergosterol (Erg), in which there are two different AmB-Erg orientations, parallel and antiparallel, as reported prev...

journal_title：Biochemistry

authors： Nakagawa Y,Umegawa Y,Matsushita N,Yamamoto T,Tsuchikawa H,Hanashima S,Oishi T,Matsumori N,Murata M

更新日期：2016-06-21 00:00:00

abstract：:A series of peptide fragments of sperm whale myoglobin, corresponding to segments of the region between the G- and H-helices of the protein, have been synthesized and their conformational preferences investigated using circular dichroism and nuclear magnetic resonance spectroscopy in aqueous solution and in solvent mi...

journal_title：Biochemistry

authors： Shin HC,Merutka G,Waltho JP,Wright PE,Dyson HJ

更新日期：1993-06-29 00:00:00

abstract：:Oncomodulin is a 108-residue, oncodevelopmental protein containing two calcium-binding sites identified as the CD- and EF-loops. The protein contains no tryptophan and only two tyrosine residues, one which is a calcium ligand in the CD-loop (Tyr-57) and one which lies in the flanking D-helix of this loop (Tyr-65). Sit...

journal_title：Biochemistry

authors： Hutnik CM,MacManus JP,Banville D,Szabo AG

更新日期：1991-07-30 00:00:00

abstract：:Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...

journal_title：Biochemistry

authors： Takano K,Aoi A,Koga Y,Kanaya S

更新日期：2013-07-16 00:00:00

abstract：:The flavoprotein nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to aldehydes and ketones, respectively, transferring electrons to oxygen to form hydrogen peroxide. The steady-state kinetic mechanism of the active flavin adenine dinucleotide-(FAD-) containing form of the enzyme has be...

journal_title：Biochemistry

authors： Gadda G,Fitzpatrick PF

更新日期：2000-02-15 00:00:00

abstract：:Two novel heme-containing peroxidases, one able to incorporate halogens into aromatic substrates and the other able to remove them, have recently been isolated from marine sources and initially characterized by Chen et al. [(1991) J. Biol. Chem. 266, 23909-23915; (1996) J. Biol. Chem. 271, 4609-4612]. The haloperoxida...

journal_title：Biochemistry

authors： Roach MP,Chen YP,Woodin SA,Lincoln DE,Lovell CR,Dawson JH

更新日期：1997-02-25 00:00:00

abstract：:The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...

journal_title：Biochemistry

authors： Srinivasan R,Fisher HF

更新日期：1985-09-24 00:00:00

abstract：:Lipoprotein(a) [Lp(a)] is a unique lipoprotein consisting of a low-density lipoprotein moiety (LDL) covalently linked to apoprotein(a). Previous work has demonstrated that Lp(a) can compete with plasminogen (PGN) for binding to endothelial and mononuclear cells and can inhibit PGN activation in cell-free systems. We h...

journal_title：Biochemistry

authors： Ezratty A,Simon DI,Loscalzo J

更新日期：1993-05-04 00:00:00

abstract：:This paper presents the crystal structure of porcine pancreatic carboxypeptidase B (pp-CpB) in complex with a variety of thiol-based inhibitors that were developed as antagonists of activated thrombin-activatable fibrinolysis inhibitor (TAFIa). Recent studies have indicated that a selective inhibitor of TAFIa could en...

journal_title：Biochemistry

authors： Adler M,Bryant J,Buckman B,Islam I,Larsen B,Finster S,Kent L,May K,Mohan R,Yuan S,Whitlow M

更新日期：2005-07-05 00:00:00

abstract：:A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...

journal_title：Biochemistry

authors： Stellwagen NC

更新日期：1983-12-20 00:00:00

abstract：:The effects of structural modifications of the flavin mononucleotide (FMN) prosthetic group of Clostridium pasteurianum flavodoxin on the kinetics of electron transfer to the oxidized form (from 5-deazariboflavin semiquinone produced by laser flash photolysis) and from the semiquinone form (to horse heart cytochrome c...

journal_title：Biochemistry

authors： Simondsen RP,Tollin G

更新日期：1983-06-07 00:00:00

abstract：:PDZ domains are among the most abundant protein modules in the known genomes. Their main function is to provide scaffolds for membrane-associated protein complexes by binding to the cytosolic, C-terminal fragments of receptors, channels, and other integral membrane proteins. Here, using both heteronuclear NMR and sing...

journal_title：Biochemistry

authors： Grembecka J,Cierpicki T,Devedjiev Y,Derewenda U,Kang BS,Bushweller JH,Derewenda ZS

更新日期：2006-03-21 00:00:00