A structural basis for the regulation of an H-NOX-associated cyclic-di-GMP synthase/phosphodiesterase enzyme by nitric oxide-bound H-NOX.


:Biofilms are surface-attached communities of bacteria enclosed in a polysaccharide matrix. Bacteria in a biofilm are extremely resistant to antibiotics. Several recent reports have linked the signaling molecule nitric oxide (NO) with biofilm dispersal. We have previously reported that an H-NOX (heme-nitric oxide/oxygen binding) protein in the biofilm-dwelling bacterium Shewanella woodyi mediates NO-induced biofilm dispersal. In S. woodyi, H-NOX (SwH-NOX) is cocistronic with a gene encoding a dual-functioning diguanylate cyclase/phosphodiesterase enzyme, designated here as HaCE (H-NOX-associated cyclic-di-GMP processing enzyme). Enzymes such as these are responsible for regulating the intracellular concentrations of cyclic-di-GMP, a secondary signaling molecule essential to biofilm formation in bacteria. We have demonstrated that NO-bound SwH-NOX regulates both enzymatic activities of SwHaCE, resulting in decreased cellular cyclic-di-GMP levels and disruption of biofilm formation. Thus, H-NOX/HaCE represents a potential drug target for regulating biofilm formation. In this work, the SwH-NOX surface residues critical for the formation of a protein complex with SwHaCE are identified using nuclear magnetic resonance, fluorescence quenching, and cosedimentation. Enzyme assays confirm this protein-protein interface and its importance for H-NOX/HaCE function.






Lahiri T,Luan B,Raleigh DP,Boon EM




Has Abstract


2014-04-08 00:00:00












  • What's in your buffer? Solute altered millisecond motions detected by solution NMR.

    abstract::To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate in comparison to MES- and HEPES-buffered references on the chemical shift perturbation an...


    pub_type: 杂志文章


    authors: Wong M,Khirich G,Loria JP

    更新日期:2013-09-17 00:00:00

  • Fluorescence-detected circular dichroism of ethidium in vivo and bound to deoxyribonucleic acid in vitro.

    abstract::Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However...


    pub_type: 杂志文章


    authors: Lamos ML,Turner DH

    更新日期:1985-06-04 00:00:00

  • Trehalose-containing lipooligosaccharides of Mycobacterium gordonae: presence of a mono-O-methyltetra-O-acyltrehalose "core" and branching in the oligosaccharide backbone.

    abstract::Past evidence has indicated that Mycobacterium gordonae, as isolated from soil and as an occasional opportunistic pathogen, exists as a serocomplex. We now demonstrate that the basis of seroreactivity and diversity is a novel series of alkali-labile, trehalose-containing lipooligosaccharides (LOS). The structures from...


    pub_type: 杂志文章


    authors: Besra GS,McNeil MR,Khoo KH,Dell A,Morris HR,Brennan PJ

    更新日期:1993-11-30 00:00:00

  • Mechanistic investigations of a ribozyme derived from the Tetrahymena group I intron: insights into catalysis and the second step of self-splicing.

    abstract::Self-splicing of Tetrahymena pre-rRNA proceeds in two consecutive phosphoryl transesterification steps. One major difference between these steps is that in the first an exogenous guanosine (G) binds to the active site, while in the second the 3'-terminal G414 residue of the intron binds. The first step has been extens...


    pub_type: 杂志文章


    authors: Mei R,Herschlag D

    更新日期:1996-05-07 00:00:00

  • Influence of histidine tag attachment on picosecond protein dynamics.

    abstract::Polyhistidine affinity tags are routinely employed as a convenient means of purifying recombinantly expressed proteins. A tacit assumption is commonly made that His tags have little influence on protein structure and function. Attachment of a His tag to the N-terminus of the robust globular protein myoglobin leads to ...


    pub_type: 杂志文章


    authors: Thielges MC,Chung JK,Axup JY,Fayer MD

    更新日期:2011-06-28 00:00:00

  • Interaction of the Saccharomyces cerevisiae alpha-factor with phospholipid vesicles as revealed by proton and phosphorus NMR.

    abstract::Proton and phosphorus-31 nuclear magnetic resonance (1H and 31P NMR) studies of the interaction between a tridecapeptide pheromone, the alpha-factor of Saccharomyces cerevisiae, and sonicated lipid vesicles are reported. 31P NMR studies demonstrate that there is interaction of the peptide with the phosphorus headgroup...


    pub_type: 杂志文章


    authors: Jelicks LA,Broido MS,Becker JM,Naider FR

    更新日期:1989-05-16 00:00:00

  • Molecular dynamics simulations of binding modes between methylene blue and DNA with alternating GC and AT sequences.

    abstract::The understanding of interactions between small molecules and DNA is crucial to design new anticancer drugs targeted to DNA. Methylene blue (MB) is a phenothiazinium dye that has shown promising results in photodynamic therapy treatment. The noncovalent binding of methylene blue to DNA was experimentally and theoretic...


    pub_type: 杂志文章


    authors: Nogueira JJ,González L

    更新日期:2014-04-15 00:00:00

  • Solution structure of oxidized cytochrome c6 from the green alga Monoraphidium braunii.

    abstract::Cytochrome c6 from Monoraphidium braunii, an 89-amino acid electron transfer protein, has been investigated by NMR in solution, in its oxidized form, at pH 7 and 300 K. By using a combination of COSY, TOCSY, and NOESY experiments, 84% of the proton resonances have been assigned. A total of 1668 experimental NOE constr...


    pub_type: 杂志文章


    authors: Banci L,Bertini I,De la Rosa MA,Koulougliotis D,Navarro JA,Walter O

    更新日期:1998-04-07 00:00:00

  • Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity.

    abstract::We have initiated a broad-based program aimed at understanding the molecular basis of fluorine specificity in enzymatic systems, and in this context, we report crystallographic and biochemical studies on a fluoroacetyl-coenzyme A (CoA) specific thioesterase (FlK) from Streptomyces cattleya. Our data establish that FlK...


    pub_type: 杂志文章


    authors: Weeks AM,Coyle SM,Jinek M,Doudna JA,Chang MC

    更新日期:2010-11-02 00:00:00

  • Kinetics of halide release of haloalkane dehalogenase: evidence for a slow conformational change.

    abstract::Haloalkane dehalogenase converts haloalkanes to their corresponding alcohols and halides. The reaction mechanism involves the formation of a covalent alkyl-enzyme complex which is hydrolyzed by water. The active site is a hydrophobic cavity buried between the main domain and the cap domain of the enzyme. The enzyme ha...


    pub_type: 杂志文章


    authors: Schanstra JP,Janssen DB

    更新日期:1996-05-07 00:00:00

  • Adenosine 5'-(gamma-thiotriphosphate): an ATP analog that should be used with caution in muscle contraction studies.

    abstract::The slowly hydrolyzed ATP analog adenosine 5'-(gamma-thiotriphosphate) (ATP gamma S) has been used in many studies of the muscle motor protein myosin in order to form a stable "weak binding" state analogous to the actin-S1-ATP complex However, the results from studies using ATP gamma S do not always agree with the res...


    pub_type: 杂志文章


    authors: Resetar AM,Chalovich JM

    更新日期:1995-12-12 00:00:00

  • Regulation of the cardiac muscle ryanodine receptor by O(2) tension and S-nitrosoglutathione.

    abstract::The cardiac and skeletal muscle sarcoplasmic reticulum ryanodine receptor Ca(2+) release channels contain thiols that are potential targets of endogenously produced reactive oxygen and nitrogen intermediates. Previously, we showed that the skeletal muscle ryanodine receptor (RyR1) has O(2)-sensitive thiols; only when ...


    pub_type: 杂志文章


    authors: Sun J,Yamaguchi N,Xu L,Eu JP,Stamler JS,Meissner G

    更新日期:2008-12-30 00:00:00

  • Interparticle interactions and structural changes of nucleosome core particles in low-salt solution.

    abstract::The structural behavior of the nucleosome core particles in the range of solvent Na+ concentration from 10.45 to 0.45 mM has been studied by small-angle neutron and synchroton radiation X-ray scattering, sedimentation, atomic absorption spectroscopy, density measurements, and circular dichroism. With decreasing salt c...


    pub_type: 杂志文章


    authors: Hirai M,Niimura N,Zama M,Mita K,Ichimura S,Tokunaga F,Ishikawa Y

    更新日期:1988-10-04 00:00:00

  • Aromatic substrate specificity of horseradish peroxidase C studied by a combined fluorescence line narrowing/energy minimization approach: the effect of localized side-chain reorganization.

    abstract::Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...


    pub_type: 杂志文章


    authors: Laberge M,Osvath S,Fidy J

    更新日期:2001-08-07 00:00:00

  • Effects of select histidine to cysteine mutations on transcriptional regulation by Escherichia coli RcnR.

    abstract::The RcnR metalloregulator represses the transcription of the Co(II) and Ni(II) exporter, RcnAB. Previous studies have shown that Co(II) and Ni(II) bind to RcnR in six-coordinate sites, resulting in derepression. Here, the roles of His60, His64, and His67 in specific metal recognition are examined. His60 and His64 corr...


    pub_type: 杂志文章


    authors: Higgins KA,Hu HQ,Chivers PT,Maroney MJ

    更新日期:2013-01-08 00:00:00

  • Irreversible inhibition of serine proteases by peptide derivatives of (alpha-aminoalkyl)phosphonate diphenyl esters.

    abstract::Peptidyl derivatives of diphenyl (alpha-aminoalkyl)phosphonates have been synthesized and are effective and specific inhibitors of serine proteases at low concentrations. Z-PheP(OPh)2 irreversibly reacts with chymotrypsin (kobsd/[I] = 1200 M-1 s-1) and does not react with two elastases. The best inhibitor for most chy...


    pub_type: 杂志文章


    authors: Oleksyszyn J,Powers JC

    更新日期:1991-01-15 00:00:00

  • Syntheses of biotinylated and dethiobiotinylated insulins.

    abstract::The 600-MHz proton spectrum of dethiobiotin (prepared from d-biotin with Raney nickel) was measured in order to gain information pertaining to its stereochemical homogeneity. The spectrum demonstrated clearly that the material is a 6:1 mixture of two stereoisomers. The cis compound, corresponding to the stereochemistr...


    pub_type: 杂志文章


    authors: Hofmann K,Zhang WJ,Romovacek H,Finn FM,Bothner-By AA,Mishra PK

    更新日期:1984-06-05 00:00:00

  • Stability and reversibility of higher ordered structure of interphase chromatin: continuity of deoxyribonucleic acid is not required for maintenance of folded structure.

    abstract::The organization of the higher order structure of chromatin has been examined in chicken erythrocyte. Chromatin solubilized during the time course of a gentle micrococcal nuclease digestion of nuclei shows a continuous variation in the distribution of molecular weights. Electron microscopy studies of large chromatin f...


    pub_type: 杂志文章


    authors: Ruiz-Carrillo A,Puigdomènech P,Eder G,Lurz R

    更新日期:1980-06-10 00:00:00

  • The Structure of the Bimolecular Complex between Amphotericin B and Ergosterol in Membranes Is Stabilized by Face-to-Face van der Waals Interaction with Their Rigid Cyclic Cores.

    abstract::Amphotericin B (AmB) is a polyene macrolide antibiotic isolated from Streptomyces nodosus. The antifungal activity of AmB can be attributed to the formation of an ion-channel assembly in the presence of ergosterol (Erg), in which there are two different AmB-Erg orientations, parallel and antiparallel, as reported prev...


    pub_type: 杂志文章


    authors: Nakagawa Y,Umegawa Y,Matsushita N,Yamamoto T,Tsuchikawa H,Hanashima S,Oishi T,Matsumori N,Murata M

    更新日期:2016-06-21 00:00:00

  • Peptide models of protein folding initiation sites. 2. The G-H turn region of myoglobin acts as a helix stop signal.

    abstract::A series of peptide fragments of sperm whale myoglobin, corresponding to segments of the region between the G- and H-helices of the protein, have been synthesized and their conformational preferences investigated using circular dichroism and nuclear magnetic resonance spectroscopy in aqueous solution and in solvent mi...


    pub_type: 杂志文章


    authors: Shin HC,Merutka G,Waltho JP,Wright PE,Dyson HJ

    更新日期:1993-06-29 00:00:00

  • Metal-induced changes in the fluorescence properties of tyrosine and tryptophan site-specific mutants of oncomodulin.

    abstract::Oncomodulin is a 108-residue, oncodevelopmental protein containing two calcium-binding sites identified as the CD- and EF-loops. The protein contains no tryptophan and only two tyrosine residues, one which is a calcium ligand in the CD-loop (Tyr-57) and one which lies in the flanking D-helix of this loop (Tyr-65). Sit...


    pub_type: 杂志文章


    authors: Hutnik CM,MacManus JP,Banville D,Szabo AG

    更新日期:1991-07-30 00:00:00

  • Evolvability of thermophilic proteins from archaea and bacteria.

    abstract::Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...


    pub_type: 杂志文章


    authors: Takano K,Aoi A,Koga Y,Kanaya S

    更新日期:2013-07-16 00:00:00

  • Iso-mechanism of nitroalkane oxidase: 1. Inhibition studies and activation by imidazole.

    abstract::The flavoprotein nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to aldehydes and ketones, respectively, transferring electrons to oxygen to form hydrogen peroxide. The steady-state kinetic mechanism of the active flavin adenine dinucleotide-(FAD-) containing form of the enzyme has be...


    pub_type: 杂志文章


    authors: Gadda G,Fitzpatrick PF

    更新日期:2000-02-15 00:00:00

  • Notomastus lobatus chloroperoxidase and Amphitrite ornata dehaloperoxidase both contain histidine as their proximal heme iron ligand.

    abstract::Two novel heme-containing peroxidases, one able to incorporate halogens into aromatic substrates and the other able to remove them, have recently been isolated from marine sources and initially characterized by Chen et al. [(1991) J. Biol. Chem. 266, 23909-23915; (1996) J. Biol. Chem. 271, 4609-4612]. The haloperoxida...


    pub_type: 杂志文章


    authors: Roach MP,Chen YP,Woodin SA,Lincoln DE,Lovell CR,Dawson JH

    更新日期:1997-02-25 00:00:00

  • Comparison of the energetics of the uncatalyzed and glutamate dehydrogenase catalyzed alpha-imino acid-alpha-amino acid interconversion.

    abstract::The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...


    pub_type: 杂志文章


    authors: Srinivasan R,Fisher HF

    更新日期:1985-09-24 00:00:00

  • Lipoprotein(a) binds to human platelets and attenuates plasminogen binding and activation.

    abstract::Lipoprotein(a) [Lp(a)] is a unique lipoprotein consisting of a low-density lipoprotein moiety (LDL) covalently linked to apoprotein(a). Previous work has demonstrated that Lp(a) can compete with plasminogen (PGN) for binding to endothelial and mononuclear cells and can inhibit PGN activation in cell-free systems. We h...


    pub_type: 杂志文章


    authors: Ezratty A,Simon DI,Loscalzo J

    更新日期:1993-05-04 00:00:00

  • Crystal structures of potent thiol-based inhibitors bound to carboxypeptidase B.

    abstract::This paper presents the crystal structure of porcine pancreatic carboxypeptidase B (pp-CpB) in complex with a variety of thiol-based inhibitors that were developed as antagonists of activated thrombin-activatable fibrinolysis inhibitor (TAFIa). Recent studies have indicated that a selective inhibitor of TAFIa could en...


    pub_type: 杂志文章


    authors: Adler M,Bryant J,Buckman B,Islam I,Larsen B,Finster S,Kent L,May K,Mohan R,Yuan S,Whitlow M

    更新日期:2005-07-05 00:00:00

  • Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels.

    abstract::A detailed study has been made of the polyacrylamide gel electrophoresis of DNA restriction fragments obtained from two plasmids, pBR322 and p82-6B. Variables studied were molecular weight, gel concentration, temperature, and electric field strength. The retardation coefficients of the larger fragments (greater than 8...


    pub_type: 杂志文章


    authors: Stellwagen NC

    更新日期:1983-12-20 00:00:00

  • Transient kinetics of redox reactions of flavodoxin: effects of chemical modification of the flavin mononucleotide prosthetic group on the dynamics of intermediate complex formation and electron transfer.

    abstract::The effects of structural modifications of the flavin mononucleotide (FMN) prosthetic group of Clostridium pasteurianum flavodoxin on the kinetics of electron transfer to the oxidized form (from 5-deazariboflavin semiquinone produced by laser flash photolysis) and from the semiquinone form (to horse heart cytochrome c...


    pub_type: 杂志文章


    authors: Simondsen RP,Tollin G

    更新日期:1983-06-07 00:00:00

  • The binding of the PDZ tandem of syntenin to target proteins.

    abstract::PDZ domains are among the most abundant protein modules in the known genomes. Their main function is to provide scaffolds for membrane-associated protein complexes by binding to the cytosolic, C-terminal fragments of receptors, channels, and other integral membrane proteins. Here, using both heteronuclear NMR and sing...


    pub_type: 杂志文章


    authors: Grembecka J,Cierpicki T,Devedjiev Y,Derewenda U,Kang BS,Bushweller JH,Derewenda ZS

    更新日期:2006-03-21 00:00:00