pH-responsive DNA-binding activity of Helicobacter pylori NikR.

abstract：:Since a major function of RNA polymerase must be to bring together substrates in the optimal configuration for internucleotide bond formation, studies have been undertaken to understand the geometrical relationship of the two substrates. A model has been constructed for the geometry of interaction of two ATP molecules...

journal_title：Biochemistry

authors： Beal RB,Pillai RP,Chuknyisky PP,Levy A,Tarien E,Eichhorn GL

更新日期：1990-06-26 00:00:00

abstract：:A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...

journal_title：Biochemistry

authors： le Coutre J,Turk E,Kaback HR,Wright EM

更新日期：2002-06-25 00:00:00

abstract：:The VWF A1 domain seems to possess two heparin binding regions (residues 565-587 and 633-648) displaying positively charged amino acids, but the overall polyanion-A1 domain interaction scheme remains essentially elusive. To probe this molecular reaction as well as the role of electrostatic forces in VWF-heparin intera...

journal_title：Biochemistry

authors： Rastegar-Lari G,Villoutreix BO,Ribba AS,Legendre P,Meyer D,Baruch D

更新日期：2002-05-28 00:00:00

abstract：:The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...

journal_title：Biochemistry

authors： Caspary WJ,Lanzo DA,Niziak C

更新日期：1982-01-19 00:00:00

abstract：:Onconase (ONC) from Rana pipiens is the smallest member of the ribonuclease A (RNase A) superfamily. Despite a tertiary structure similar to RNase A, ONC is distinguished by an extremely high thermodynamic stability. In the present paper we have probed the significance of three structural regions, which exhibit struct...

journal_title：Biochemistry

authors： Arnold U,Schulenburg C,Schmidt D,Ulbrich-Hofmann R

更新日期：2006-03-21 00:00:00

abstract：:Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradia...

journal_title：Biochemistry

authors： Zhao B,Lamb DC,Lei L,Kelly SL,Yuan H,Hachey DL,Waterman MR

更新日期：2007-07-31 00:00:00

abstract：:Circumsporozoite (CS) protein is a predominant surface antigen of malaria sporozoites, the infective form of the parasite, and has been used for making anti-malaria vaccines. For the first time we have examined the interaction of CS protein with various glycosaminoglycans in real time using surface plasmon resonance (...

journal_title：Biochemistry

authors： Rathore D,McCutchan TF,Garboczi DN,Toida T,Hernáiz MJ,LeBrun LA,Lang SC,Linhardt RJ

更新日期：2001-09-25 00:00:00

abstract：:Flavohemoglobins (flavoHbs) are enzymes that operate primarily as nitric oxide dioxygenases and shuttle thereby electrons among NAD(P)H, FAD, heme, and a ligated redox-active substrate such as O(2). They function in the bacterial defense against nitrosative stress and are therefore considered as targets for new antibi...

journal_title：Biochemistry

authors： El Hammi E,Warkentin E,Demmer U,Limam F,Marzouki NM,Ermler U,Baciou L

更新日期：2011-02-22 00:00:00

abstract：:Myelin basic protein (MBP) binds to negatively charged lipids on the cytosolic surface of oligodendrocyte membranes and is most likely responsible for adhesion of these surfaces in the multilayered myelin sheath. It can also polymerize actin, bundle F-actin filaments, and bind actin filaments to lipid bilayers through...

journal_title：Biochemistry

authors： Boggs JM,Rangaraj G,Gao W,Heng YM

更新日期：2006-01-17 00:00:00

abstract：:Biological membranes exhibit an asymmetric distribution of phospholipids. Phosphatidylserine (PS) is an acidic phospholipid that is found almost entirely on the interior of the cell where it is important for interaction with many cellular components. A less well understood phenomenon is the asymmetry of the neutral ph...

journal_title：Biochemistry

authors： Bazzi MD,Youakim MA,Nelsestuen GL

更新日期：1992-02-04 00:00:00

abstract：:Folylpolyglutamate synthetase was purified 30,000-150,000-fold from hog liver. Purification required the use of protease inhibitors, and the protein was purified to homogeneity in two forms. Both forms of the enzyme were monomers of Mr 62,000 and had similar specific activities. The specific activity of the homogeneou...

journal_title：Biochemistry

authors： Cichowicz DJ,Shane B

更新日期：1987-01-27 00:00:00

abstract：:We report cloning, expression in Escherichia coli, and purification of cytochrome P450 from a deep-sea bacterium Photobacterium profundum strain SS9 (P450-SS9). The enzyme, which is predominately high spin (86%) in the absence of any added ligand, binds fatty acids and their derivatives and exhibits the highest affini...

journal_title：Biochemistry

authors： Sineva EV,Davydov DR

更新日期：2010-12-21 00:00:00

abstract：:We have examined the effects on redox kinetics of changing the reduction potential of the mu-oxo-bridged binuclear iron center in octameric hemerythrin (Hr) from Phascolopsis gouldii. The opportunity to examine such effects is provided by the availability of mu-sulfidomethemerythrin (mu-S2-metHr), whose [Fe(III),Fe(II...

journal_title：Biochemistry

authors： Pearce LL,Utecht RE,Kurtz DM Jr

更新日期：1987-12-29 00:00:00

abstract：:Factor XII was purified approximately 14 000-fold from bovine plasma by ammonium sulfate fractionation followed by heparin-agarose, DEAE-Sephadex, CM-cellulose, arginine-agarose, and benzamidine-agarose column chromatography. By this method, about 15 mg of protein was purified from 15 L of plasma with an overall yield...

journal_title：Biochemistry

authors： Fujikawa K,Walsh KA,Davie EW

更新日期：1977-05-17 00:00:00

abstract：:The effects of incubation of erythrocyte ghosts under various conditions (ionic strength or addition of ankyrin, diamines, or ATP) on the lateral motion of band 3 in the membranes were studied by using the fluorescence photobleaching recovery technique. Incubation of ghosts with exogenous ankyrin increased the immobil...

journal_title：Biochemistry

authors： Tsuji A,Ohnishi S

更新日期：1986-10-07 00:00:00

abstract：:Despite significant progress in the past decade, questions still remain about the complete structural, dynamic, and thermodynamic effect of the cis-syn cyclobutane pyrimidine dimer lesion (hereafter called the thymine dimer) on double-stranded genomic DNA. We examined a 19-mer oligodeoxynucleotide duplex containing a ...

journal_title：Biochemistry

authors： Rumora AE,Kolodziejczak KM,Malhowski Wagner A,Núñez ME

更新日期：2008-12-09 00:00:00

abstract：:The human, cytosolic enzyme isocitrate dehydrogenase 1 (IDH1) reversibly converts isocitrate to α-ketoglutarate (αKG). Cancer-associated somatic mutations in IDH1 result in a loss of this normal function but a gain in a new or neomorphic ability to convert αKG to the oncometabolite 2-hydroxyglutarate (2HG). To improve...

journal_title：Biochemistry

authors： Rendina AR,Pietrak B,Smallwood A,Zhao H,Qi H,Quinn C,Adams ND,Concha N,Duraiswami C,Thrall SH,Sweitzer S,Schwartz B

更新日期：2013-07-02 00:00:00

abstract：:Experimental and theoretical double-mutant cycles have been used to investigate a salt bridge in the N-terminal domain of the protein L9. Aspartic acid 23 is the only acidic residue involved in a well-defined pairwise interaction, namely, a partially solvent-exposed salt bridge with the protonated N-terminus of the pr...

journal_title：Biochemistry

authors： Luisi DL,Snow CD,Lin JJ,Hendsch ZS,Tidor B,Raleigh DP

更新日期：2003-06-17 00:00:00

abstract：:Thiazolinyl imine reductases catalyze the NADPH-dependent reduction of a thiazoline to a thiazolidine, a required step in the formation of the siderophores yersiniabactin (Yersinia spp.) and pyochelin (Pseudomonas aeruginosa). These stand-alone nonribosomal peptide tailoring domains are structural homologues of sugar ...

journal_title：Biochemistry

authors： Meneely KM,Ronnebaum TA,Riley AP,Prisinzano TE,Lamb AL

更新日期：2016-09-27 00:00:00

abstract：:Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...

journal_title：Biochemistry

authors： Gu Y,Xiao B,Wargo HL,Bucher MH,Singh SV,Ji X

更新日期：2003-02-04 00:00:00

abstract：:Rad51 protein, a eukaryotic homologue of RecA protein, forms a filamentous complex with DNA and catalyzes homologous recombination. We have analyzed the structure of Xenopus Rad51 protein (XRad51.1) in solution by small-angle neutron scattering (SANS). The measurements showed that XRad51.1 forms a helical filament ind...

journal_title：Biochemistry

authors： Ellouze C,Kim HK,Maeshima K,Tuite E,Morimatsu K,Horii T,Mortensen K,Nordén B,Takahashi M

更新日期：1997-11-04 00:00:00

abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...

journal_title：Biochemistry

authors： Brandes R,Vold RR,Vold RL,Kearns DR

更新日期：1986-11-18 00:00:00

abstract：:Apolipoprotein B (apoB) is a nonexchangeable apolipoprotein that dictates the synthesis of chylomicrons and very low density lipoproteins. ApoB is the major protein in low density lipoprotein, also known as the "bad cholesterol" that is directly implicated in atherosclerosis. It has been suggested that the N-terminal ...

journal_title：Biochemistry

authors： Jiang ZG,Gantz D,Bullitt E,McKnight CJ

更新日期：2006-10-03 00:00:00

abstract：:Specific binding of glutamate to its carrier was investigated by using cytoplasmic membrane vesicles prepared from Escherichia coli B. The binding activity was specifically affected by the Na+ and H+ concentrations of the medium. Cytoplasmic membrane vesicles from the mutant strain 36-39 that is defective in the Na+-d...

journal_title：Biochemistry

authors： Fujimura T,Yamato I,Anraku Y

更新日期：1983-04-12 00:00:00

abstract：:Elongation factor Tu (EF-Tu) is a highly conserved GTPase that is responsible for supplying the aminoacylated tRNA to the ribosome. Upon binding to the ribosome, EF-Tu undergoes GTP hydrolysis, which drives a major conformational change, triggering the release of aminoacylated tRNA to the ribosome. Using a combination...

journal_title：Biochemistry

authors： Lai J,Ghaemi Z,Luthey-Schulten Z

更新日期：2017-11-14 00:00:00

abstract：:Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Dev...

journal_title：Biochemistry

authors： Holden JK,Dejam D,Lewis MC,Huang H,Kang S,Jing Q,Xue F,Silverman RB,Poulos TL

更新日期：2015-07-07 00:00:00

abstract：:Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...

journal_title：Biochemistry

authors： Taylor A,Peltier CZ,Jahngen EG Jr,Laxman E,Szewczuk Z,Torre FJ

更新日期：1992-04-28 00:00:00

abstract：:Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...

journal_title：Biochemistry

authors： Vadas EB,Melançon P,Braun PE,Galley WC

更新日期：1981-05-26 00:00:00

abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

journal_title：Biochemistry

authors： Parry RJ,Muscate A,Askonas LJ

更新日期：1991-10-15 00:00:00

abstract：:The diiron active sites of the purple acid phosphatases from porcine uterus (also called uteroferrin, Uf) and bovine spleen (BSPAP) and their complexes with tungstate are compared by 1H NMR and NOE techniques. The paramagnetically shifted features of the 1H NMR spectrum of reduced BSPAP are similar to those of reduced...

journal_title：Biochemistry

authors： Wang Z,Ming LJ,Que L Jr,Vincent JB,Crowder MW,Averill BA

更新日期：1992-06-16 00:00:00