Abstract:
:We have examined the effects on redox kinetics of changing the reduction potential of the mu-oxo-bridged binuclear iron center in octameric hemerythrin (Hr) from Phascolopsis gouldii. The opportunity to examine such effects is provided by the availability of mu-sulfidomethemerythrin (mu-S2-metHr), whose [Fe(III),Fe(III)]met----[Fe(II),Fe(III)]semi-met reduction potential is approximately 200 mV higher than that of methemerythrin (metHr). We have used, as redox partners to Hr, a set of metal complexes and the heme proteins deoxymyoglobin (Mb) and cytochrome b5. The latter protein from P. gouldii is a presumed physiological redox partner of Hr. Similar kinetics at pH 8 in the presence or absence of the allosteric effector perchlorate suggest reduction of the iron atom closer to the outer surface of each subunit in the Hr octamer during the met----semi-met transformation. For all reducing agents, the experimentally determined ratio of second-order rate constants for reductions of mu-S2-metHr and metHr, k12(mu-S2-met)/k12(met), is close to the value of 40 predicted by the simple Marcus relation for "outer-sphere" electron transfer. For oxidations of (semi-met)RHr and mu-S2-semi-metHr, the predicted value of 40 for k12[(semi-met)R]/k12(mu-S2-semi-met) is closely approximated when Fe(CN)6(3-) is used as oxidant. The ionic strength dependence of the second-order rate constant suggests electrostatic interactions of opposite charges during reduction of metHr by P. gouldii cytochrome b5.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Pearce LL,Utecht RE,Kurtz DM Jrdoi
10.1021/bi00400a033subject
Has Abstractpub_date
1987-12-29 00:00:00pages
8709-17issue
26eissn
0006-2960issn
1520-4995journal_volume
26pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The local environment of the transmembrane and C-terminal domain of M13 major coat protein was probed by site-directed ESR spin labeling when the protein was introduced into three membrane-mimicking systems, DOPC vesicles, sodium cholate micelles, and SDS micelles. For this purpose, we have inserted unique cysteine re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961770j
更新日期:1996-12-03 00:00:00
abstract::CD23/Fc epsilonRII, the low-affinity receptor for IgE, is a multifunctional protein of importance in blood cell development and the immune system. We have studied the interaction of CD23 with IgE in solution using hydrodynamic methods applied to recombinant fragments of both ligands: sCD23, corresponding to the solubl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961231e
更新日期:1997-02-25 00:00:00
abstract::Although the mechanism by which the steroidogenic acute regulatory protein (StAR) promotes steroidogenesis has been studied extensively, it remains incompletely characterized. Because structural analysis has revealed a hydrophobic sterol-binding pocket (SBP) within StAR, this study sought to examine the regulatory rol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401125v
更新日期:2013-10-15 00:00:00
abstract::A new approach to studying membrane topology and permissive sites in membrane proteins expressed in Escherichia coli is described. The method is based on in vitro transposition of mini-Tn5 derivatives bearing dual pho-lac reporters [Alexeyev, M. F., and Winkler, H. H. (1999) J. Mol. Biol. 285, 1503-1513]. Two mini-Tn5...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011813i
更新日期:2002-01-08 00:00:00
abstract::Electron spin echo envelope modulation (ESEEM) spectroscopy has been applied to the determination of the number of water molecules coordinated to the metal in the binary complex of staphylococcal nuclease with Mn2+, to the ternary enzyme-Mn2+-3',5'-pdTp complex, and to ternary complexes of a number of mutant enzymes i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00421a010
更新日期:1988-10-18 00:00:00
abstract::Although Mg(2+) is the cation that functions as the cofactor for the nucleotidyl transfer reaction for almost all DNA polymerases, Mn(2+) can also serve, but when it does, the degree of base discrimination exhibited by most DNA polymerases (pols) is diminished. Metal ions other than Mg(2+) or Mn(2+) can also act as co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01350
更新日期:2016-05-10 00:00:00
abstract::The proton-translocating NADH:ubiquinone oxidoreductase of respiratory chains (complex I) contains one flavin mononucleotide and five EPR-detectable iron-sulfur clusters as redox groups. Because of the number of conserved motifs typical for binding iron-sulfur clusters and the high content of iron and acid-labile sulf...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0026977
更新日期:2001-05-22 00:00:00
abstract::The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00633a003
更新日期:1977-07-12 00:00:00
abstract::Transport of protons and solutes across mitochondrial membranes is essential for many physiological processes. However, neither the proton-pumping respiratory chain complexes nor the mitochondrial secondary active solute transport proteins have been characterized electrophysiologically in their native environment. In ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1011755
更新日期:2010-12-07 00:00:00
abstract::Azurin from Pseudomonas aeruginosa and two mutants where the methionine ligand has been mutated have been studied in order to directly investigate the functional and structural significance of this ligand in the blue copper proteins. Reduction potentials, X-ray absorption fine structure (XAFS), electron paramagnetic r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a013
更新日期:1993-03-02 00:00:00
abstract::The enzyme PvdQ (E.C. 3.5.1.97) from Pseudomonas aeruginosa is an N-terminal nucleophile hydrolase that catalyzes the removal of an N-myristyl substituent from a biosynthetic precursor of the iron-chelating siderophore pyoverdine. Inhibitors of pyoverdine biosynthesis are potential antibiotics since iron is essential ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501086s
更新日期:2014-10-28 00:00:00
abstract::Tat, an essential human immunodeficiency virus type 1 protein interacts with the transactivation response element (TAR) and stimulates transcription from the viral long-terminal repeat (LTR). Blockage of Tat-TAR interaction halts viral transcription and hence replication. We have found that polyamide nucleic acid (PNA...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000708q
更新日期:2000-09-26 00:00:00
abstract::Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi2015822
更新日期:2011-12-20 00:00:00
abstract::In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8020668
更新日期:2009-06-16 00:00:00
abstract::tRNA-guanine transglycosylase (TGT) is a key enzyme involved in the post-transcriptional modification of certain tRNAs in their anticodon wobble positions with queuine. To maintain the correct Watson-Crick base pairing properties of the wobble base (and hence proper translation of the genetic code), TGT must recognize...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051863d
更新日期:2006-01-17 00:00:00
abstract::Two site-directed mutant enzymes of the class A beta-lactamase from Staphylococcus aureus PC1 were produced with the goal of blocking the site that in the native enzyme is occupied by the proposed hydrolytic water molecule. The crystal structures of these two mutant enzymes, N170Q and N170M, have been determined and r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi962242a
更新日期:1996-12-24 00:00:00
abstract::Inherently curved DNA segments, associated with short runs of adenines, have been identified in many gene regulatory regions, yet their physiological significance remains unknown. The observations reported in this study indicate that intrinsically bent nucleic acid fragments are characterized by substantially attenuat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00123a019
更新日期:1992-03-03 00:00:00
abstract::We have examined the C cluster in type II CO dehydrogenase (CODH) from Carboxydothermus hydrogenformans using Ni K-edge X-ray absorption near edge spectroscopy and extended X-ray absorption fine structure (EXAFS) spectroscopy. The enzyme was studied under three conditions: "as-isolated" and after treatment with CO or ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036104e
更新日期:2004-07-20 00:00:00
abstract::Amyloid fibrils comprising primarily the peptides A beta 40 and A beta 42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9904149
更新日期:1999-07-13 00:00:00
abstract::The reduction of CBrCl3 by the heme-heme oxygenase complex forms dissociable and covalently bound heme products. No such products are formed with mesoheme in which the heme vinyl substituents are replaced by ethyl groups. The dissociable heme products are chromatographically similar but not identical to those obtained...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972720x
更新日期:1998-03-03 00:00:00
abstract::The inhibition mechanism of serpins requires a change in structure to entrap the target proteinase as a stable acyl-enzyme complex. Although it has generally been assumed that reactive center loop insertion and associated conformational change proceeds in a concerted manner, this has not been demonstrated directly. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi025967p
更新日期:2002-10-08 00:00:00
abstract::Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00704
更新日期:2018-10-02 00:00:00
abstract::Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0508845
更新日期:2005-08-23 00:00:00
abstract::Metal displacement reactions of Cd7MT with Ag+ or Cu+ and interprotein metal exchange reactions between Cd7MT and Ag12MT or Cu12MT were studied by 111Cd NMR. Titration of 111Cd7MT with Ag+ indicates that Ag+ binds preferentially to the beta-domain of the protein to form the metal hybrid species, (Cd4)alpha(Ag6)beta MT...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961401n
更新日期:1996-11-05 00:00:00
abstract::We have characterized by EPR the interaction of the Em,7 = -50 mV [4Fe-4S] cluster of Escherichia coli DMSO reductase (DmsABC) with a menaquinol (MQH2) binding site. Potentiometric titrations indicate that in DmsAB(C102S)C, the Em,7 = -50 mV [4Fe-4S] cluster is replaced by an Em,7 = +260 mV [3Fe-4S] cluster. The Q-poo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951584y
更新日期:1996-03-12 00:00:00
abstract::Although the synthesis of RNA from a DNA template is (and must be) a generally very stable process to enable transcription of kilobase transcripts, it has long been known that during initial transcription of the first 8-10 bases of RNA complexes are relatively unstable, leading to the release of short abortive RNA tra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200620q
更新日期:2011-08-16 00:00:00
abstract::The direct electrochemistry of the cytochrome c/cytochrome b5 and cytochrome c/plastocyanin complexes has been investigated at edge-plane graphite and modified gold electrode surfaces, which are selective for one of the two components of the complex. Electrochemical response of one protein at an otherwise electrostati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00465a010
更新日期:1990-04-03 00:00:00
abstract::The Z and E isomers of 6-(methoxymethylene)-penicillanic acid have been synthesized, and their interaction with the RTEM beta-lactamase has been studied. The Z isomer is an inhibitor and an inactivator of the enzyme, and there is some similarity between its behavior and that of other mechanism-based inactivators such ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00319a025
更新日期:1984-11-20 00:00:00
abstract::The interaction of myristoyllysophosphatidylcholine with bovine myelin basic protein at pH 7.4 and 4.5, I = 0.48, has been investigated by a recycling partition equilibrium technique with Bio-Gel P-2 as the gel phase. Important points to emerge from this direct binding study are that it is a monomeric (not micellar) a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00378a002
更新日期:1987-02-24 00:00:00
abstract::A double mutant of the single-domain protein barstar having a single tryptophan (W53) was made by mutating the remaining two tryptophans (W38 and W44) into phenylalanines. W53 is buried in the core of barstar. Time-resolved fluorescence of the mutant barstar (W38FW44F) showed that W53 has a single fluorescence lifetim...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9603478
更新日期:1996-07-16 00:00:00