Identification of two tetranuclear FeS clusters on the ferredoxin-type subunit of NADH:ubiquinone oxidoreductase (complex I).

abstract：:Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 were subjected to a double soaking procedure, in which they were first soaked in a solution containing the inhibitor acarbose and subsequently in a solution containing maltohexaose. The refined structure of the resultin...

journal_title：Biochemistry

authors： Strokopytov B,Knegtel RM,Penninga D,Rozeboom HJ,Kalk KH,Dijkhuizen L,Dijkstra BW

更新日期：1996-04-02 00:00:00

abstract：:Phospholipase B1 (PLB1), secreted by the pathogenic yeast Cryptococcus neoformans, has an established role in virulence. Although the mechanism of its phospholipase B, lysophospholipase, and lysophospholipase transacylase activities is unknown, it possesses lipase, subtilisin protease aspartate, and phospholipase moti...

journal_title：Biochemistry

authors： Jones PM,Turner KM,Djordjevic JT,Sorrell TC,Wright LC,George AM

更新日期：2007-09-04 00:00:00

abstract：:Choline acetyltransferase (EC 2.3.1.6) catalyzes the biosynthesis of acetylcholine according to the following chemical equation: acetyl-CoA + choline in equilibrium to acetylcholine + CoA. In addition to nervous tissue, primate placenta is the only other animal source which contains appreciable acetylcholine and its b...

journal_title：Biochemistry

authors： Roskoski R Jr,Lim CT,Roskoski LM

更新日期：1975-11-18 00:00:00

abstract：:Supported phospholipid bilayers prepared by Langmuir-Blodgett techniques were introduced recently as a new model membrane system [Tamm, L.K., & McConnell, H.M. (1985) Biophys. J. 47, 105-113]. Here, supported bilayers are applied to study the lateral diffusion and lateral distribution of membrane-bound monoclonal anti...

journal_title：Biochemistry

authors： Tamm LK

更新日期：1988-03-08 00:00:00

abstract：:In mammals, numerous precursors of antibacterial peptides with unrelated sequences share a similar prosequence of 94-114 residues, termed the cathelin-like domain. The cathelin-like domain of protegrin-3 (ProS) was overexpressed in Escherichia coli and uniformly labeled with (15)N or (15)N and (13)C, and its three-dim...

journal_title：Biochemistry

authors： Yang Y,Sanchez JF,Strub MP,Brutscher B,Aumelas A

更新日期：2003-04-29 00:00:00

abstract：:Internal fatty acylation of proteins is a recognized means of modifying biological behavior. Escherichia coli hemolysin A (HlyA), a toxic protein, is transcribed as a nontoxic protein and made toxic by internal acylation of two lysine residue epsilon-amino groups; HlyC catalyzes the acyl transfer from acyl-acyl carrie...

journal_title：Biochemistry

authors： Trent MS,Worsham LM,Ernst-Fonberg ML

更新日期：1999-07-06 00:00:00

abstract：:Sendai virus particles are able to fuse with Pronase-neuraminidase-treated human erythrocyte membranes as well as with vesicles obtained from chromaffin granules of bovine medulla. Fusion is inferred either from electron microscopic studies or from the observation that incubation of fluorescently labeled (bearing octa...

journal_title：Biochemistry

authors： Citovsky V,Laster Y,Schuldiner S,Loyter A

更新日期：1987-06-30 00:00:00

abstract：:A quartz-crystal microbalance (QCM) technique was applied to analyze effects of site-directed mutagenesis of a glycosidase (isomalto-dextranase) on the hydrolysis mechanism of the substrate binding (k(on), k(off), and K(d)) and the catalytic process (k(cat)), separately, by using a dextran-immobilized QCM in buffer so...

journal_title：Biochemistry

authors： Nihira T,Mizuno M,Tonozuka T,Sakano Y,Mori T,Okahata Y

更新日期：2005-07-12 00:00:00

abstract：:The sensor kinase CitA and the response regulator CitB of Klebsiella pneumoniae form the paradigm of a subfamily of bacterial two-component regulatory systems that are capable of sensing tri- or dicarboxylates in the environment and then induce transporters for the uptake of these compounds. We recently showed that th...

journal_title：Biochemistry

authors： Gerharz T,Reinelt S,Kaspar S,Scapozza L,Bott M

更新日期：2003-05-20 00:00:00

abstract：:The self-association of rabbit muscle phosphofructokinase at pH 7.0 was investigated by velocity sedimentation. The process was demonstrated to be in a rapid, dynamic equilibrium. The concentration dependence of the weight-average sedimentation coefficient was monitored within the range of 10-750 microgram/mL. The sed...

journal_title：Biochemistry

authors： Hesterberg LK,Lee JC

更新日期：1981-05-12 00:00:00

abstract：:Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...

journal_title：Biochemistry

authors： Himmel M,Van Der Ven PF,Stöcklein W,Fürst DO

更新日期：2003-01-21 00:00:00

abstract：:ALY is a ubiquitously expressed nuclear protein which interacts with proteins such as TAP that are involved in export of mRNA from the nucleus to the cytoplasm, as well as with proteins that bind the T cell receptor alpha gene enhancer. ALY has also been shown to bind mRNA and to co-localize in the nucleus with compon...

journal_title：Biochemistry

authors： Pérez-Alvarado GC,Martínez-Yamout M,Allen MM,Grosschedl R,Dyson HJ,Wright PE

更新日期：2003-06-24 00:00:00

abstract：:15N NMR spectroscopy was used to examine the active-site histidyl residue of alpha-lytic protease in peptide boronic acid inhibitor complexes. Two distinct types of complexes were observed: (1) Boronic acids that are analogues of substrates form complexes in which the active-site imidazole ring is protonated and both ...

journal_title：Biochemistry

authors： Bachovchin WW,Wong WY,Farr-Jones S,Shenvi AB,Kettner CA

更新日期：1988-10-04 00:00:00

abstract：:The complete amino acid sequences of two basic proline-rich proteins, IB-1 and IB-6, from human parotid saliva have been determined. Fragments for sequence analysis were obtained by enzymatic digestions. The proteins have molecular weights of 9571 (IB-1) and 11,530 (IB-6) and contain 34 and 39 mol % proline, respectiv...

journal_title：Biochemistry

authors： Kauffman D,Hofmann T,Bennick A,Keller P

更新日期：1986-05-06 00:00:00

abstract：:Photolyases reverse the effects of UV light on cells by converting cyclobutane dipyrimidine photoproducts (pyrimidine dimers, Pyr mean value of Pyr) into pyrimidine monomers in a light-dependent reaction. Previous work has suggested that, based on substrate preference, there are two classes of photolyase: DNA photolya...

journal_title：Biochemistry

authors： Kim ST,Sancar A

更新日期：1991-09-03 00:00:00

abstract：:DNA gyrase is the target of a number of antibacterial agents, including the coumarins and the cyclothialidines. To extend our understanding of the mechanism of action of these compounds, we have examined the previously published crystal structures of the complexes between the 24 kDa fragment of GyrB and coumarin and c...

journal_title：Biochemistry

authors： Kampranis SC,Gormley NA,Tranter R,Orphanides G,Maxwell A

更新日期：1999-02-16 00:00:00

abstract：:The ZFY protein is a member of one of the most interesting classes of polydactyl zinc finger proteins. It has a domain of 13 tandem zinc fingers that is organized with an internal repeat of odd-even finger pairs. It has been proposed that each finger pair interacts with six base pairs within a turn of the double helix...

journal_title：Biochemistry

authors： Grants J,Flanagan E,Yee A,Romaniuk PJ

更新日期：2010-02-02 00:00:00

abstract：:Membrane proteins are embedded in a lipid bilayer and maintain strong interactions with lipid molecules. Tightly bound lipids are responsible for vertical positioning and integration of proteins in the membrane and for assembly of multisubunit complexes and occasionally act as substrates. In this work we present the l...

journal_title：Biochemistry

authors： Wöhri AB,Wahlgren WY,Malmerberg E,Johansson LC,Neutze R,Katona G

更新日期：2009-10-20 00:00:00

abstract：:Escherichia coli MutS protein, which is required for mismatch repair, has a slow ATPase activity that obeys Michalelis-Menten kinetics. At 37 degrees C, the steady-state turnover rate for ATP hydrolysis is 1.0 +/- 0.3 min(-1) per monomer equivalent with a K(m) of 33 +/- 6 microM. Hydrolysis is competitively inhibited ...

journal_title：Biochemistry

authors： Bjornson KP,Allen DJ,Modrich P

更新日期：2000-03-21 00:00:00

abstract：:CP47 is a pigment-protein complex in the core of photosystem II that tranfers excitation energy to the reaction center. Here we report on a spectroscopic investigation of the isolated CP47 complex. By deconvoluting the 77 K absorption and linear dichroism, red-most states at 683 and 690 nm have been identified with os...

journal_title：Biochemistry

authors： De Weerd FL,Palacios MA,Andrizhiyevskaya EG,Dekker JP,Van Grondelle R

更新日期：2002-12-24 00:00:00

abstract：:The folding of the two-domain bacterial chaperone PapD has been studied to develop an understanding of the relationship between individual domain folding and the formation of domain-domain interactions. PapD contains six phenylalanine residues, four in the N-terminal domain and two in the C-terminal domain. To examine...

journal_title：Biochemistry

authors： Bann JG,Frieden C

更新日期：2004-11-02 00:00:00

abstract：:Dihydrofolate and dihydropteroylpolyglutamates inhibit pig liver methylenetetrahydrofolate reductase. In all cases the inhibition is linearly competitive with respect to methylenetetrahydrofolate. The Ki values decrease with each additional glutamyl residue from one to six, from a value of 6.5 microM for dihydrofolate...

journal_title：Biochemistry

authors： Matthews RG,Baugh CM

更新日期：1980-05-13 00:00:00

abstract：:Streptomyces griseus trypsin has been isolated from Pronase by ion-exchange chromatography on CM-Sephadex and SE-Sephadex. The isolated enzyme was homogeneous by the criteria tested except for a low degree of contamination by an enzyme with nontryptic activity. The latter could be partially resolved by chromatography ...

journal_title：Biochemistry

authors： Olafson RW,Smillie LB

更新日期：1975-03-25 00:00:00

abstract：:Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...

journal_title：Biochemistry

authors： Mecham RP,Hinek A,Entwistle R,Wrenn DS,Griffin GL,Senior RM

更新日期：1989-05-02 00:00:00

abstract：:We report a new observation of the role of Escherichia coli single-strand DNA binding protein (SSB) in synthesis of primer RNA (pRNA) catalyzed by.E.coli primase on the SSB-coated phage G4oric template. Using a set of ATP priming substrates with reactive groups attached to the 5' gamma-phosphate on different length "a...

journal_title：Biochemistry

authors： Godson GN,Mustaev AA,Sun W

更新日期：1998-03-17 00:00:00

abstract：:Horseradish peroxidase (HRP) was oxidized by IrCl6(2-) to a mixture of compounds I and II, the rate of oxidation and the ratio of the mixture being greatly affected by pH (Hayashi & Yamazaki, 1979). Oxidation of HRP by IrCl6(2-) in the presence of fluoride was significantly accelerated. This resulted in the formation ...

journal_title：Biochemistry

authors： Farhangrazi ZS,Sinclair R,Powers L,Yamazaki I

更新日期：1995-11-21 00:00:00

abstract：:Miscoding properties induced by estrogen quinone-derived DNA adducts were analyzed using an in vitro experimental system to quantify base substitutions and deletions. Site-specifically modified oligodeoxynucleotides containing a single N2-(2-hydroxyestron-6-yl)-2'-deoxyguanosine (2-OHE1-N2-dG) or N6-(2-hydroxyestron-6...

journal_title：Biochemistry

authors： Terashima I,Suzuki N,Dasaradhi L,Tan CK,Downey KM,Shibutani S

更新日期：1998-09-29 00:00:00

abstract：:A well-defined region of pancreatic and other secreted phospholipase A2 (PLA2), which we call the i-face, makes a molecular contact with the interface to facilitate and control the events and processivity of the interfacial catalytic turnover cycles. The structural features of the i-face and its allosteric relationshi...

journal_title：Biochemistry

authors： Yu BZ,Janssen MJ,Verheij HM,Jain MK

更新日期：2000-05-16 00:00:00

abstract：:FixL is a sensor histidine kinase having a heme-containing domain as an O(2) sensing site. In the study presented here, Ile209 and Ile210 located near the heme iron of the heme domain of Rhizobium meliloti FixL (RmFixL) were mutated, and the mutational effects on the regulation of the kinase activity and the heme pock...

journal_title：Biochemistry

authors： Mukai M,Nakamura K,Nakamura H,Iizuka T,Shiro Y

更新日期：2000-11-14 00:00:00

abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...

journal_title：Biochemistry

authors： Sun M,Leyh TS

更新日期：2006-09-26 00:00:00