Osmotic swelling allows fusion of sendai virions with membranes of desialyzed erythrocytes and chromaffin granules.

abstract：:By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...

journal_title：Biochemistry

authors： Singh AK,Ekka MK,Kaushik A,Pandya V,Singh RP,Banerjee S,Mittal M,Singh V,Kumaran S

更新日期：2017-09-19 00:00:00

abstract：:The stoichiometry of the nitrogenase ATP-dependent H2 evolution and ecetylene reduction reactions using S2O4(2-) as an electron source was studied by various techniques. For each mole of S2O4(2-) oxidized to 2SO3(2-) by the enzyme-catalyzed reactions at 25 degrees and pH 8, 1 mol of H2 (1 mol of ethylene for acetylene...

journal_title：Biochemistry

authors： Watt GD,Bulen WA,Burns A,Hadfield KL

更新日期：1975-09-23 00:00:00

abstract：:The interaction of nile red (NR) with apomyoglobin (ApoMb) in the native (pH 7) and molten globule (pH 4) states was investigated using experimental and computational methods. NR binds to hydrophobic locations in ApoMb with higher affinity (K(d) = 25 +/- 5 microM) in the native state than in the molten globule state (...

journal_title：Biochemistry

authors： Polverini E,Cugini G,Annoni F,Abbruzzetti S,Viappiani C,Gensch T

更新日期：2006-04-25 00:00:00

abstract：:CaATPase from rabbit sarcoplasmic reticulum (SR) has been isolated, purified, and reconstituted into unilamellar vesicles with 1,2-dipentadecanoylphosphatidylcholine (DPePC) and acyl-chain-perdeuterated 1,2-dipalmitoylphosphatidylcholine (DPPC-d62). For total lipid:protein mole ratios between 25 and 70, a constant "ha...

journal_title：Biochemistry

authors： Senak L,Mendelsohn R

更新日期：1993-06-22 00:00:00

abstract：:Human DNA polymerase kappa (pol kappa) has a sequence significantly homologous with that of Escherichia coli DNA polymerase IV (pol IV). We used a truncated form of human pol kappa (pol kappaDeltaC) and full-length pol IV to explore the miscoding properties of these enzymes. Oligodeoxynucleotides, modified site-specif...

journal_title：Biochemistry

authors： Suzuki N,Ohashi E,Hayashi K,Ohmori H,Grollman AP,Shibutani S

更新日期：2001-12-18 00:00:00

abstract：:The human intestinal Caco-2 cell line, described as enterocyte-like in a number of studies, was examined for its ability to carry out the metabolism of vitamin A normally required in the absorptive process. Caco-2 cells contained cellular retinol-binding protein II, a protein which is abundant in human villus-associat...

journal_title：Biochemistry

authors： Quick TC,Ong DE

更新日期：1990-12-18 00:00:00

abstract：:Previous experiments have shown that a Synechocystis sp. PCC 6803 mutant (delta psbO) lacking the extrinsic manganese-stabilizing protein (MSP) exhibits impaired, but significant levels of H2O-splitting activity [Burnap, R., & Sherman, L.A. (1991) Biochemistry 30, 440-446]. [14C]DCMU-binding experiments now show that ...

journal_title：Biochemistry

authors： Burnap RL,Shen JR,Jursinic PA,Inoue Y,Sherman LA

更新日期：1992-08-18 00:00:00

abstract：:The interaction of single chain urokinase with its receptor accelerates plasminogen activator activity on cell surfaces and induces intracellular signalling in several cell types. To date, no physiologic inhibitor of this binding has been identified. We report that the binding of scuPA to its cellular receptor is inhi...

journal_title：Biochemistry

authors： al-Roof Higazi A,Aceto JF,Kniss D,Upson R,Cohen R,Dichek DA,Cines DB

更新日期：1996-05-28 00:00:00

abstract：:The binding of ethidium bromide to the self-complementary deoxytetranucleotides containing guanine and cytosine bases has been studied by circular dichroism, visible absorption, and fluorescence spectroscopies. The circular dichroism spectrum of each of the four deoxytetranucleotides was measured and compared to the s...

journal_title：Biochemistry

authors： Kastrup RV,Young MA,Krugh TR

更新日期：1978-11-14 00:00:00

abstract：:We show that eight different serine proteinases--bovine chymotrypsins A and B, porcine pancreatic elastase I, proteinase K, Streptomyces griseus proteinases A and B, and subtilisins BPN' and Carlsberg--interact with turkey ovomucoid third domain at the same Leu18-Glu19 peptide bond, the reactive site of the inhibitor....

journal_title：Biochemistry

authors： Ardelt W,Laskowski M Jr

更新日期：1985-09-24 00:00:00

abstract：:Membrane vesicles from Mycobacterium phlei contain carrier proteins for proline, glutamine, and glutamic acid. The transport of proline is Na+ dependent and required substrate oxidation. A proline carrier protein was solubilized from the membrane vesicles by treatment with cholate and Triton X-100. Electron microscopi...

journal_title：Biochemistry

authors： Lee SH,Cohen NS,Jacobs AJ,Brodie AF

更新日期：1979-05-29 00:00:00

abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...

journal_title：Biochemistry

authors： Haapalainen AM,Meriläinen G,Pirilä PL,Kondo N,Fukao T,Wierenga RK

更新日期：2007-04-10 00:00:00

abstract：:To reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class Ib ribonucleotide reductase (RNR) must be activated via a pathway that involves redox protein(s). The reduced flavoprotein NrdI is an important protein in this pathway, as it reduces dioxygen to superoxide. Superoxide then reacts with...

journal_title：Biochemistry

authors： Lofstad M,Gudim I,Hammerstad M,Røhr ÅK,Hersleth HP

更新日期：2016-09-13 00:00:00

abstract：:Filamin A (FLNA) is a ubiquitously expressed actin cross-linking protein and a scaffold of numerous binding partners to regulate cell proliferation, migration, and survival. FLNA is a homodimer, and each subunit has an N-terminal actin-binding domain followed by 24 immunoglobulin-like repeats (R). FLNA mediates mechan...

journal_title：Biochemistry

authors： Wang L,Nakamura F

更新日期：2019-11-26 00:00:00

abstract：:Here, we biochemically characterize Dictyostelium myosin II mutants that were previously phenotypically selected following random mutagenesis and shown to lie in the actin binding domain [Patterson, B., & Spudich, J. A. (1996) Genetics 143, 801-810]. We show that the conditional loss of myosin-dependent activity in vi...

journal_title：Biochemistry

authors： Giese KC,Spudich JA

更新日期：1997-07-15 00:00:00

abstract：:Spatially localized differences in the conformational dynamics of the rubredoxins from the hyperthermophile Pyrococcus furiosus (Pf) and the mesophile Clostridium pasteurianum (Cp) are monitored via amide exchange measurements. As shown previously for the hyperthermophile protein, nearly all backbone amides of the Cp ...

journal_title：Biochemistry

authors： Hernández G,LeMaster DM

更新日期：2001-12-04 00:00:00

abstract：:The structure of [14-38]Abu, a variant of bovine pancreatic trypsin inhibitor (BPTI) with only the 14-38 disulfide bridge intact, has been analyzed by two-dimensional 1H and 1H-15N NMR. Except for the 18-24, 29-35 antiparallel beta-sheet, residues in all regions of the molecule give two exchange cross peaks for each 1...

journal_title：Biochemistry

authors： Barbar E,Barany G,Woodward C

更新日期：1995-09-12 00:00:00

abstract：:The hydrogen-bonded ring NH nuclear magnetic resonance (NMR) spectra of several transfer ribonucleic acid (RNA) species have been examined with particular emphasis on the extreme low-field portion. Betwen --13.8 and --15 ppm there are two extra resonances which are not derived from cloverleaf base pairs. A combined ap...

journal_title：Biochemistry

authors： Hurd RE,Reid BR

更新日期：1979-09-04 00:00:00

abstract：:The binding of adenosine 3',5'-cyclic monophosphate (cAMP) and its nonfunctional analogue, guanosine 3',5'-cyclic monophosphate (cGMP), to the adenosine 3',5'-cyclic monophosphate receptor protein (CRP) from Escherichia coli was investigated by means of fluorescence and isothermal titration calorimetry (ITC) at pH 7.8...

journal_title：Biochemistry

authors： Lin SH,Lee JC

更新日期：2002-10-01 00:00:00

abstract：:Anthrax toxin receptor 1 (ANTXR1)/tumor endothelial marker 8 (TEM8) is one of two known proteinaceous cell surface anthrax toxin receptors. A metal ion dependent adhesion site (MIDAS) present in the integrin-like inserted (I) domain of ANTXR1 mediates the binding of the anthrax toxin subunit, protective antigen (PA). ...

journal_title：Biochemistry

authors： Ramey JD,Villareal VA,Ng C,Ward SC,Xiong JP,Clubb RT,Bradley KA

更新日期：2010-08-31 00:00:00

abstract：:The relative quantitative importance of the factors that determine the fidelity of translation is largely unknown, which makes predicting the extent to which the degeneracy of the genetic code can be broken challenging. Our strategy of using orthogonal tRNA/aminoacyl tRNA synthetase pairs to precisely direct the incor...

journal_title：Biochemistry

authors： Schmitt MA,Biddle W,Fisk JD

更新日期：2018-05-15 00:00:00

abstract：:Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...

journal_title：Biochemistry

authors： Tyagi NK,Goyal P,Kumar A,Pandey D,Siess W,Kinne RK

更新日期：2005-11-29 00:00:00

abstract：:Two-dimensional (2D) proton magnetic resonance techniques used in conjunction with laser photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy have been applied to studying the kringle 4 domain from human plasminogen at 360 MHz. Out of 11 potential CIDNP-sensitive aromatic side chains, only 5...

journal_title：Biochemistry

authors： De Marco A,Zetta L,Petros AM,Llinás M,Boelens R,Kaptein R

更新日期：1986-12-02 00:00:00

abstract：:The reaction of nitric oxide (NO) with fast cytochrome bo from Escherichia coli has been studied by electronic absorption, MCD, and EPR spectroscopy. Titration of the enzyme with NO showed the formation of two distinct species, consistent with NO binding stoichiometries of 1:1 and 2:1 with observed dissociation consta...

journal_title：Biochemistry

authors： Butler CS,Seward HE,Greenwood C,Thomson AJ

更新日期：1997-12-23 00:00:00

abstract：:A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through th...

journal_title：Biochemistry

authors： Wandschneider E,Hammack BN,Bowler BE

更新日期：2003-09-16 00:00:00

abstract：:The insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/MPR) is a type I glycoprotein that mediates both the intracellular sorting of lysosomal enzymes bearing mannose 6-phosphate (Man-6-P) residues to the lysosome and the bioavailability of IGF-II. The extracytoplasmic region of the IGF-II/MPR contains...

journal_title：Biochemistry

authors： Marron-Terada PG,Hancock MK,Haskins DJ,Dahms NM

更新日期：2000-03-07 00:00:00

abstract：:The peptide alpha 1(III)-CB9 was prepared and purified from human liver, and its amino acid sequence was determined. Automated Edman degradation of the intact peptide and peptides derived from selective cleavage with hydroxylamine and digestions with trypsin, thermolysin, and Staph V8 protease enabled determination of...

journal_title：Biochemistry

authors： Seyer JM,Kang AH

更新日期：1981-04-28 00:00:00

abstract：:The melibiose permease of Salmonella typhimurium (MelB(St)) catalyzes symport of melibiose with Na(+), Li(+), or H(+), and bioinformatics analysis indicates that a conserved Gly117 (helix IV) is part of the Na(+)-binding site. We mutated Gly117 to Ala, Pro, Trp, or Arg; the effects on melibiose transport and binding o...

journal_title：Biochemistry

authors： Guan L,Jakkula SV,Hodkoff AA,Su Y

更新日期：2012-04-03 00:00:00

abstract：:The binding of agonists, antagonists, and the acetylcholinesterase inhibitor, eserine, to the nicotinic acetylcholine receptor from Torpedo californica has been monitored by the fluorescence changes of two extrinsic probes that have been covalently attached to the receptor protein. Although both probes, 5-(iodoacetami...

journal_title：Biochemistry

authors： Dunn SM,Raftery MA

更新日期：1993-08-24 00:00:00

abstract：:The Autographa californica multinucleocapsid nuclear polyhedrosis virus genome contains nine homologous region (hr1, hr1a, hr2, hr2a, hr3, hr4a, hr4b, hr4c, and hr5) sequences that are thought to be involved in viral replication and activation of transcription. Our results show that the 750 bp hr1 sequence is capable ...

journal_title：Biochemistry

authors： Venkaiah B,Viswanathan P,Habib S,Hasnain SE

更新日期：2004-06-29 00:00:00