Abstract：
:A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through the four cooperative folding units of cytochrome c. The double mutant cycle starts with the iso-1-cytochrome c variant AcTM, a variant with no surface histidines and with asparagine at position 52. Isoleucine is added singly at position 52, AcTMI52 variant, as are the surface histidines, AcHX variants, where X indicates the histidine sequence position. The double mutant variants, AcHXI52, provide the remaining corner of the double mutant cycle. The stabilities of all variants were determined by guanidine hydrochloride denaturation and interaction energies were calculated between position 52 and each histidine site. Six of the seven double mutants show additive (AcH33I52, AcH39I52, AcH54I52, AcH89I52, and AcH100I52) stability effects or weak interaction energies (AcH73I52) of the histidine mutations and the N52I mutation, consistent with cooperative effects on protein folding and stability being sparsely distributed through the protein structure. The AcH26I52 variant shows a strong favorable interaction energy, 2.0 +/- 0.5 kcal/mol, between the N52I mutation in one substructure and the addition of His 26 to an adjacent substructure. The data are consistent with an entropic stabilization of the intersubstructure hydrogen bond between His 26 and Glu 44 by the Ile 52 mutation.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Wandschneider E,Hammack BN,Bowler BEdoi
10.1021/bi034958tsubject
Has Abstractpub_date
2003-09-16 00:00:00pages
10659-66issue
36eissn
0006-2960issn
1520-4995journal_volume
42pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:A specific requirement for lipids, particularly cardiolipin (CL), in cytochrome c oxidase (CcO) has been reported in many previous studies using mainly in vitro lipid removal approaches in mammalian systems. Our accompanying paper shows that CcO produced in markedly CL-depleted Rhodobacter sphaeroides displays wild-ty...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1017039
更新日期：2011-05-17 00:00:00
abstract：:Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi301369b
更新日期：2013-02-05 00:00:00
abstract：:The Sup35 protein of Saccharomyces cerevisiae forms a prion that generates the [PSI(+)] phenotype. Its NM region governs prion status, forming self-seeding amyloid fibers in vivo and in vitro. A tryptophan mutant of Sup35 (NM(F117W)) was used to probe its aggregation. Four indicators of aggregation, Trp 117 maximum em...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9000276
更新日期：2009-07-28 00:00:00
abstract：:Brief exposure of purple membrane (PM) to dilute Triton X-100 eliminates the actinic light effect on the relative amounts of fast M (Mf) and slow M (Ms) intermediates and alters the character and kinetics of the photocycle, without destroying the native BR trimers (Mukhopadhyay et al., 1994). Particular membrane lipid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi960738m
更新日期：1996-07-16 00:00:00
abstract：:LL-23 is a natural peptide corresponding to the 23 N-terminal amino acid residues of human host defense cathelicidin LL-37. LL-23 demonstrated, compared to LL-37, a conserved ability to induce the chemokine MCP-1 in human peripheral blood mononuclear cells, a lack of ability to suppress induction of the pro-inflammato...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2016266
更新日期：2012-01-17 00:00:00
abstract：:The early steps of the proposed mechanistic pathway for dehydroquinate synthase have been probed with a series of substrate analogues. These analogues, 3-9, are structurally prohibited from undergoing the beta-elimination of inorganic phosphate that represents the committed step in the conversion of the substrate 3-de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a010
更新日期：1989-09-19 00:00:00
abstract：:The D-alanyl-D-alanine-adding enzyme encoded by the murF gene catalyzes the ATP-dependent formation of UDP-N-acetylmuramyl-L-gamma-D-Glu-meso-diaminopimelyl-D-Ala-D-Ala (UDP-MurNAc-tripeptide). MurF has been cloned from Escherichia coli and expressed as a glutathione S-transferase (GST) fusion using the tac promoter-b...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961872+
更新日期：1996-12-17 00:00:00
abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi902045p
更新日期：2010-03-09 00:00:00
abstract：:Adenosyltransferases (ATRs) catalyze the synthesis of the reactive cobalt-carbon bond found in coenzyme B(12) or 5'-deoxyadenosylcobalamin (AdoCbl), which serves as a cofactor for a number of isomerases. The reaction involves a reductive adenosylation of cob(II)alamin in which an electron delivered by a reductase redu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900454s
更新日期：2009-06-16 00:00:00
abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6026192
更新日期：2007-04-10 00:00:00
abstract：:Thermostable direct hemolysin (TDH) of Vibrio parahemolyticus is a membrane-damaging pore-forming toxin with potent cytolytic/cytotoxic activity. TDH exists as a tetramer consisting of protomers with a core β-sandwich domain, flanked by an 11-amino acid long N-terminal region (NTR). This NTR could not be modeled in th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00937
更新日期：2020-02-04 00:00:00
abstract：:The diffusion of protons at the immediate vicinity of (less than 10 A from) a phospholipid membrane is studied by the application of the laser-induced proton pulse. A light-sensitive proton emitter (8-hydroxypyrene-1,3,6-trisulfonate) was trapped exclusively in the hydration layers of multilamellar vesicles made of eg...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00433a029
更新日期：1989-04-04 00:00:00
abstract：:Pantothenate kinase (PanK) catalyzes the first step of the universal five-step coenzyme A (CoA) biosynthetic pathway. The recently characterized type III PanK (PanK-III, encoded by the coaX gene) is distinct in sequence, structure and enzymatic properties from both the long-known bacterial type I PanK (PanK-I, exempli...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7018578
更新日期：2008-02-05 00:00:00
abstract：:The properties of the high-affinity single-stranded DNA (ssDNA) binding state of Escherichia coli recA protein have been studied. We find that all of the nucleoside triphosphates that are hydrolyzed by recA protein induce a high-affinity ssDNA binding state. The effect of ATP binding to recA protein was partially sepa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00404a021
更新日期：1988-02-23 00:00:00
abstract：:Class II MHC glycoproteins bind short (7-25 amino acid) peptides in an extended type II polyproline-like conformation and present them for immune recognition. Because empty MHC is unstable, measurement of the rate of the second-order reaction between peptide and MHC is challenging. In this report, we use dissociation ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9921337
更新日期：2000-02-08 00:00:00
abstract：:The catalytic mechanism of valyl-tRNA synthetase from baker's yeast has been investigated by pre-steady-state and steady-state kinetic measurements and end product dissociation studies. The pre-steady-state kinetics show a lag period during the early time when the reaction is started with free enzyme. The preincubatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00511a001
更新日期：1981-04-14 00:00:00
abstract：:The head of the P22 bacteriophage is interrupted by a unique dodecameric portal vertex that serves as a conduit for the entrance and exit of the DNA. Here, the in vitro unfolding/refolding processes of the portal protein of P22 were investigated at different temperatures (1, 25, and 37 degrees C) through the use of ur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700006d
更新日期：2007-06-26 00:00:00
abstract：:Second-harmonic light scattering (SHS) permits characterization of membrane-specific molecular transport in living cells. Herein, we demonstrate the use of time-resolved SHS for quantifying chemically induced enhancements in membrane permeability. As proof of concept, we examine the enhanced permeability of the cytopl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00600
更新日期：2015-07-28 00:00:00
abstract：:The structure of a 22 amino acid peptide, TPPI [Nedved, M. L., Gottlieb, P. A., & Moe, G. R. (1994) Nucleic Acids Res. 22, 5024-5030], that is similar to the proline repeat segment of the replication arrest protein, Tus, has been determined by 1H NMR in 50% trifluroethanol. The structure is a novel left-handed helix h...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952419l
更新日期：1996-01-23 00:00:00
abstract：:The early redox events involved in iron reduction and mobilization in mammalian ferritin have been investigated by several techniques. Sedimentation velocity measurements of ferritin samples with altered core sizes, prepared by partial reduction and Fe2+ chelation, suggest two different events occur during iron loss f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00430a033
更新日期：1989-02-21 00:00:00
abstract：:This research was undertaken to distinguish between local and global unfolding in the reversible thermal denaturation of bovine pancreatic ribonclease A (RNase A). Local unfolding was monitored by steady-state and time-resolved fluorescence of nine mutants in each of which a single tryptophan was substituted for a wil...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001945w
更新日期：2001-01-09 00:00:00
abstract：:PQQ is an exogenous, tricyclic, quino-cofactor for a number of bacterial dehydrogenases. The final step of PQQ formation is catalyzed by PqqC, a cofactorless oxidase. This study focuses on the activation of molecular oxygen in an enzyme active site without metal or cofactor and has identified a specific oxygen binding...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi1015474
更新日期：2011-03-08 00:00:00
abstract：:The tRNA(His) guanylyltransferase (Thg1) catalyzes the incorporation of a single guanosine residue at the -1 position (G(-1)) of tRNA(His), using an unusual 3'-5' nucleotidyl transfer reaction. Thg1 and Thg1 orthologs known as Thg1-like proteins (TLPs), which catalyze tRNA repair and editing, are the only known enzyme...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201397f
更新日期：2012-01-10 00:00:00
abstract：:Homology modeling was used to identify two particular residues, Glu175 and Ala176, in Pseudomonas stutzeri phosphite dehydrogenase (PTDH) as the principal determinants of nicotinamide cofactor (NAD(+) and NADP(+)) specificity. Replacement of these two residues by site-directed mutagenesis with Ala175 and Arg176 both s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi035018b
更新日期：2003-10-14 00:00:00
abstract：:It has been shown by genetic analysis that the external and internal invertases from Saccharomyces cerevisiae share a common structural gene [Taussig, R., & Carlson, M. (1983) Nucleic Acids Res. 11, 1943-1954]. However, the only amino acid composition of these two forms of invertase reported to date has revealed exten...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a014
更新日期：1985-10-22 00:00:00
abstract：:The ferritin IRE, a highly conserved (96-99% in vertebrates) mRNA translation regulatory element in animal mRNA, was studied by molecular modeling (using MC-SYM and DOCKING) and by NMR spectroscopy. Cobalt(III) hexammine was used to model hydrated Mg2+. IRE isoforms in other mRNAs regulate mRNA translation or stabilit...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9719814
更新日期：1998-02-10 00:00:00
abstract：:The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971022+
更新日期：1997-09-30 00:00:00
abstract：:A combination of mutagenesis, calorimetry, kinetics, and small-angle X-ray scattering (SAXS) has been used to study the mechanism of ligand binding energy propagation through human cytochrome P450 reductase (CPR). Remarkably, the energetics of 2',5'-ADP binding to R597 at the FAD-binding domain are affected by mutatio...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700596s
更新日期：2007-07-17 00:00:00
abstract：:Heme-copper oxidases are membrane-bound proteins that catalyze the reduction of O(2) to H(2)O, a highly exergonic reaction. Part of the free energy of this reaction is used for pumping of protons across the membrane. The ba(3) oxidase from Thermus thermophilus presumably uses a single proton pathway for the transfer o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300132t
更新日期：2012-06-05 00:00:00
abstract：:The lateral stalk of ribosomes constitutes the GTPase-associated center and is responsible for recruiting translation factors to the ribosomes. The eukaryotic stalk contains a P-complex, in which one molecule of uL10 (formerly known as P0) protein binds two copies of P1/P2 heterodimers. Unlike bacterial uL10, eukaryot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00528
更新日期：2019-09-10 00:00:00
