Abstract：
:Brief exposure of purple membrane (PM) to dilute Triton X-100 eliminates the actinic light effect on the relative amounts of fast M (Mf) and slow M (Ms) intermediates and alters the character and kinetics of the photocycle, without destroying the native BR trimers (Mukhopadhyay et al., 1994). Particular membrane lipids are removed during the Triton treatment, and adding back an extract of membrane lipids can repair most of the affected photocycle behavior (Dracheva et al., 1996). This paper defines conditions which are important in the reconstitution procedure, using a group of quantitative parameters which measure the extents of damage and repair. Circular dichroism in both the UV and visible ranges shows that Triton can disturb both the secondary structure of BR and its ability to polymerize into trimers. Whereas the damage to protein conformation could be reversed by lipids alone, the formation of trimers and recovery of normal photocycle behavior required both lipids and a high salt concentration.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Mukhopadhyay AK,Dracheva S,Bose S,Hendler RWdoi
10.1021/bi960738msubject
Has Abstractpub_date
1996-07-16 00:00:00pages
9245-52issue
28eissn
0006-2960issn
1520-4995pii
bi960738mjournal_volume
35pub_type
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