Duplex oligodeoxyribonucleotides cross-linked by mitomycin C at a single site: synthesis, properties, and cross-link reversibility.

abstract：:The specificity of trypsin for Arg- and Lys-containing substrates depends upon the presence of Asp189 at the base of the primary binding pocket. The crystal structure of anionic rat trypsin D189S complexed with BPTI reveals that removal of the aspartate side chain permits the binding of a well-ordered acetate ion in a...

journal_title：Biochemistry

authors： Perona JJ,Hedstrom L,Wagner RL,Rutter WJ,Craik CS,Fletterick RJ

更新日期：1994-03-22 00:00:00

abstract：:In two recent papers, we reported the effects of several point mutations on the thermodynamics of the thermal unfolding of the lysozyme of phage T4 as determined by differential scanning calorimetry. The mutants studied were R96H [Kitamura, S., & Sturtevant, J.M. (1989) Biochemistry 28, 3788-3792] and T157 replaced by...

journal_title：Biochemistry

authors： Hu CQ,Kitamura S,Tanaka A,Sturtevant JM

更新日期：1992-02-18 00:00:00

abstract：:The backbone dynamics of Escherichia coli ribonuclease HI (RNase HI) in the picosecond to nanosecond time scale were characterized by a combination of measurements of 15N-NMR relaxation (T1, T2, and NOE), analyzed by a model-free approach, and molecular dynamics (MD) simulation in water. The MD simulations in water we...

journal_title：Biochemistry

authors： Yamasaki K,Saito M,Oobatake M,Kanaya S

更新日期：1995-05-23 00:00:00

abstract：:Src SH3 is a small all-beta-sheet protein composed of a single domain. We studied the folding behavior of src SH3 at various conditions by circular dichroism (CD), fluorescence, and X-ray solution scattering methods. On the src SH3 folding pathway, an alpha-helix-rich intermediate appeared not only at subzero temperat...

journal_title：Biochemistry

authors： Li J,Shinjo M,Matsumura Y,Morita M,Baker D,Ikeguchi M,Kihara H

更新日期：2007-05-01 00:00:00

abstract：:The proteasome inhibitors, lactacystin and N-acetyl-leucyl-leucyl-norlucinal, caused a rapid and near-complete loss of approximately 22-23-kDa ubiquitinated nucleoproteins, which we have identified as monoubiquitinated nucleosomal histones H2A and H2B by immunological and two-dimensional electrophoretic techniques. In...

journal_title：Biochemistry

authors： Mimnaugh EG,Chen HY,Davie JR,Celis JE,Neckers L

更新日期：1997-11-25 00:00:00

abstract：:The apolipoprotein apoE plays a key role in cholesterol and lipid metabolism. There are three isoforms of this protein, one of which, apoE4, is the major risk factor for Alzheimer's disease. At micromolar concentrations all lipid-free apoE isoforms exist primarily as monomers, dimers, and tetramers. However, the molec...

journal_title：Biochemistry

authors： Garai K,Baban B,Frieden C

更新日期：2011-04-05 00:00:00

abstract：:A series of human lymphoblastoid cell lines derived from nonleukemic donors are known to be cysteine prototrophs (cys+), while several lymphoblastoid lines derived from leukemic donors are cysteine auxotrophs (cys-). We have tested representative cell lines of each type for their content of cystathionase enzyme activi...

journal_title：Biochemistry

authors： Glode LM,Kriegler MP,Livingston DM

更新日期：1981-03-03 00:00:00

abstract：:The quaternary structures of Escherichia coli DNA-dependent RNA polymerase holenzyme (alpha 2 beta beta' sigma) and core enzyme (alpha 2 beta beta') have been investigated by chemical cross-linking with a cleavable bifunctional reagent, methyl 4-mercaptobutyrimidate, and noncleavable reagents, dimethyl suberimidate an...

journal_title：Biochemistry

authors： Hillel Z,Wu CW

更新日期：1977-07-26 00:00:00

abstract：:An alanine racemase encoded by a gene from the thermophilic Gram-positive bacterium Bacillus stearothermophilus is overproduced to 0.3% of the soluble protein when carried on plasmid pICR4 in Escherichia coli [Inagaki, K., Tanizawa, K., Badet, B., Walsh, C. T., Tanaka, H., & Soda, K. (1986) Biochemistry (third paper o...

journal_title：Biochemistry

authors： Badet B,Inagaki K,Soda K,Walsh CT

更新日期：1986-06-03 00:00:00

abstract：:Phosphorescence from the lone tryptophan residue has been studied to monitor the interaction of myelin basic protein with phosphatidylserine vesicles. Spectral shifts in the phosphorescence of the protein in a glycerol-buffer (70:30 w/w) solvent at low temperature are consistent with fluorescence data obtained under a...

journal_title：Biochemistry

authors： Vadas EB,Melançon P,Braun PE,Galley WC

更新日期：1981-05-26 00:00:00

abstract：:Various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-Bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; AMP, NAD+, and NADH markedly decreased the rate of inactivation. Some omega-halogenated carboxyamid...

journal_title：Biochemistry

authors： Fries RW,Bohlken DP,Blakley RT,Plapp BV

更新日期：1975-11-18 00:00:00

abstract：:X-ray studies of the proofreading 3',5'-exonuclease site of the large (Klenow) fragment of DNA polymerase I have detected a binuclear metal complex consisting of a pentacoordinate metal (site A) which shares a ligand, Asp-355, with an octahedral metal (site B) [Freemont, P. S., Friedman, J. M., Beese, L. S., Sanderson...

journal_title：Biochemistry

authors： Han H,Rifkind JM,Mildvan AS

更新日期：1991-11-19 00:00:00

abstract：:The interaction of the so-called fusion peptide of the human immunodeficiency virus gp41 envelope glycoprotein with the target cell membrane is believed to trigger the fusion process which allows the entry of the virus into the cell. Many studies on the interaction of the fusion peptide with biological membranes have ...

journal_title：Biochemistry

authors： Buzón V,Padrós E,Cladera J

更新日期：2005-10-11 00:00:00

abstract：:Sterol carrier protein 2 like 2 from Aedes aegypti (AeSCP2L2) plays an important role in lipid transport in mosquitoes for its routine metabolic processes. Repeated unsuccessful attempts to crystallize ligand free SCP2L2 prompted us to undertake nuclear magnetic resonance (NMR) spectroscopy to determine its three-dime...

journal_title：Biochemistry

authors： Singarapu KK,Ahuja A,Potula PR,Ummanni R

更新日期：2016-09-06 00:00:00

abstract：:Mutagenesis and cross-linking measurements have identified specific contact interactions between the cytosolic and the transmembrane sequences of phospholamban (PLB) and the Ca-ATPase, and in conjunction with the high-resolution structures of PLB and the Ca-ATPase, have been used to construct models of the PLB-ATPase ...

journal_title：Biochemistry

authors： Li J,Xiong Y,Bigelow DJ,Squier TC

更新日期：2004-01-20 00:00:00

abstract：:Detailed information on room-temperature structure and oxidation state of the Photosystem II (PS II) manganese complex is needed to put mechanistic considerations on solid grounds. Because previously this information had not been available, the tetranuclear manganese complex was investigated by X-ray absorption spectr...

journal_title：Biochemistry

authors： Meinke C,Solé VA,Pospisil P,Dau H

更新日期：2000-06-20 00:00:00

abstract：:The stacking of the bacteriochlorophyll (BChl) c macrocycles and the role of water in forming an aggregate sheet, in chlorosome, were examined by means of (13)C NMR spectroscopy, the measurement of the X-ray diffraction pattern, and (25)Mg NMR spectroscopy. (1) The stacking of the macrocycles, i.e., weakly overlapped ...

journal_title：Biochemistry

authors： Kakitani Y,Koyama Y,Shimoikeda Y,Nakai T,Utsumi H,Shimizu T,Nagae H

更新日期：2009-01-13 00:00:00

abstract：:The role of the tryptophan residues in the substrate-binding and catalytic mechanism of an enzymatically active C-terminal fragment of Pseudomonas aeruginosa exotoxin A was studied by individually or jointly replacing these residues with phenylalanine. Substitution of W-466 decreased the ADP-ribosyltransferase and NAD...

journal_title：Biochemistry

authors： Beattie BK,Prentice GA,Merrill AR

更新日期：1996-12-03 00:00:00

abstract：:Resonance Raman spectra of squid rhodopsin have been obtained under a variety of temperature and illumination conditions. The data have been characterized in terms of spectral contributions from squid rhodopsin, isorhodopsin, bathorhodopsin, lumirhodopsin, mesorhodopsin, P-465, and acid metarhodopsin. The results are ...

journal_title：Biochemistry

authors： Sulkes M,Lewis A,Marcus MA

更新日期：1978-10-31 00:00:00

abstract：:X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...

journal_title：Biochemistry

authors： Cronin CN,Kirsch JF

更新日期：1988-06-14 00:00:00

abstract：:The conformation of the GM3 ganglioside, Neu5Ac alpha 2-3Gal beta 1-4Glc beta 1-1 Cer, and its analogs containing the Neu5Gc or Neu4Ac5Gc residues (Gc = glycolyl, CH2OHCO) was investigated in Me2SO-d6 solution with the aid of a distance-mapping procedure based on rotating-frame NOE contacts, with hydroxyl protons bein...

journal_title：Biochemistry

authors： Siebert HC,Reuter G,Schauer R,von der Lieth CW,Dabrowski J

更新日期：1992-08-04 00:00:00

abstract：:Tropomyosin is postulated to confer stability to actin filaments in nonmuscle cells. We have found that a nonmuscle tropomyosin isolated from the intestinal epithelium can directly stabilize actin filaments by slowing depolymerization from the pointed, or slow-growing, filament end. Kinetics of elongation and depolyme...

journal_title：Biochemistry

authors： Broschat KO,Weber A,Burgess DR

更新日期：1989-10-17 00:00:00

abstract：:Nitridomanganese(V) protoporphyrin IX was prepared by hypochlorite oxidation of the corresponding manganese(III) protoporphyrin IX derivative in the presence of ammonium ion and by photolysis of the corresponding azidomanganese(III) complex. Myoglobin and horseradish peroxidase containing this novel protoporphyrin der...

journal_title：Biochemistry

authors： Tsubaki M,Hori H,Hotta T,Hiwatashi A,Ichikawa Y,Yu NT

更新日期：1987-08-11 00:00:00

abstract：:GPIbalpha is an integral membrane protein of the GPIb-IX-V complex found on the platelet surface that interacts with the A1 domain of von Willebrand factor (vWF-A1). The interaction of GPIbalpha with vWF-A1 under conditions of high shear stress is the first step in platelet-driven thrombus formation. Phage display was...

journal_title：Biochemistry

authors： Benard SA,Smith TM,Cunningham K,Jacob J,DeSilva T,Lin L,Shaw GD,Kriz R,Kelleher KS

更新日期：2008-04-22 00:00:00

abstract：:The kinetics of ATP-induced Ca2+ uptake by vesicular dispersions of sarcoplasmic reticulum were determined with a time resolution of about 10 ms, depending on the temperature. Ca2+ uptake was initiated by the addition of ATP through the flash photolysis of P3-1-(2-nitrophenyl)-ethyl adenosine 5'-triphosphate utilizing...

journal_title：Biochemistry

authors： Pierce DH,Scarpa A,Topp MR,Blasie JK

更新日期：1983-11-08 00:00:00

abstract：:The mechanism of DNA polymerase beta-catalyzed nucleotidyl transfer consists of chemical steps involving primer 3' OH deprotonation, nucleophilic attack, and pyrophosphate leaving-group elimination, preceded by dNTP binding which induces a large-amplitude conformational change for Watson-Crick nascent base pairs. Ambi...

journal_title：Biochemistry

authors： Sucato CA,Upton TG,Kashemirov BA,Osuna J,Oertell K,Beard WA,Wilson SH,Florián J,Warshel A,McKenna CE,Goodman MF

更新日期：2008-01-22 00:00:00

abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...

journal_title：Biochemistry

authors： Ramreddy T,Sen S,Rao BJ,Krishnamoorthy G

更新日期：2003-10-21 00:00:00

abstract：:Using solution peptide synthesis, we have made three series of N alpha-formylated homooligopeptides, from the dipeptide to the heptapeptide, derived from L-methionine, L-norleucine, and S-methyl-L-cysteine and related to the chemotactic peptide N alpha-formylmethionylleucylphenylalanine. Compounds were prepared to det...

journal_title：Biochemistry

authors： Toniolo C,Bonora GM,Showell H,Freer RJ,Becker EL

更新日期：1984-02-14 00:00:00

abstract：:The chemical mechanism of the phosphoribosyltransferases (PRTases), although largely unknown, may proceed either via a concerted direct-transfer mechanism or with a two-step mechanism involving a carboxonium-like intermediate. To study this question, we have cloned the Salmonella typhimurium pyrE gene, coding for the ...

journal_title：Biochemistry

authors： Bhatia MB,Vinitsky A,Grubmeyer C

更新日期：1990-11-20 00:00:00

abstract：:While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...

journal_title：Biochemistry

authors： Xin X,Xi L,Tu SC

更新日期：1994-10-11 00:00:00