当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > A carbon-13 nuclear magnetic resonance study of hyperactive and hypoactive derivatives of luteinizing hormone-releasing hormone: des-Gly-NH210-[DLeu6]LH-RH ethylamide and des-Gly-NH210-[L-Leu6]LH-RH ethylamide.

A carbon-13 nuclear magnetic resonance study of hyperactive and hypoactive derivatives of luteinizing hormone-releasing hormone: des-Gly-NH210-[DLeu6]LH-RH ethylamide and des-Gly-NH210-[L-Leu6]LH-RH ethylamide.

Abstract：

:The carbon-13 (13C) nuclear magnetic resonance (NMR) behavior of the hyperactive analogue of luteinizing-hormone-releasing hormone (LH-RH), des-Gly-NH2(10)-[D-Leu6]LH-RH ethylamide, has been studied. The spectra are compared with those of the hypoactive des-Gly-NH2(10)-[L-Leu6]LH-RH ethylamide. Spin-lattice relaxation times (T1) of 13C at natural abundance have been obtained for both peptides at 25 and 68 MHz in aqueous solution and used as monitors of flexibility at each carbon atom in the peptide backbone and side chains. The [D-Leu6]-and [L-Leu6]-sub-stituted analogues show equal degrees of freedom within the peptide backbone and side chains. Substitution of the glycine amide moiety in position 10 by an ethylamide group causes an increase in flexibility of the backbone at that position. The increase in flexibility is not transmitted to the adjacent prolyl residue. The C-terminal peptide link at position 10 is proposed to restrict the flexibility of the prolyl residue in position 9, thus maintaining the motional characteristics of the LH-RH peptide backbone in the des-Gly-NH2(10)-[Leu6]LH-RH ethylamide analogues. There were no significant differences in the 13C chemical shifts or T1 values between the hypoactive and hyperactive analogues in aqueous solution which would explain the differences in biological activity. Interaction between receptor and hormone may thus be required to induce, or select, the biologically active conformation of the hormone.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Deslauriers R,Komoroski RA,Levy GC,Seely JH,Smith IC

doi

10.1021/bi00666a020

subject

Has Abstract

pub_date

1976-10-19 00:00:00

pages

4672-5

issue

21

eissn

0006-2960

issn

1520-4995

journal_volume

15

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Calcium-sensitive binding of heavy meromyosin to regulated actin requires light chain 2 and the head-tail junction.

    abstract：:Sedimentation in a preparative ultracentrifuge was used to determine the affinity of heavy meromyosin, HMM, for regulated actin, F-actin plus troponin-tropomyosin, in the presence of MgATP at pH 7.0, 20 degrees C, and mu = 18 mM. HMM was prepared from vertebrate striated muscle myosin by a mild chymotryptic digestion....

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00275a003

    authors： Wagner PD,Stone DB

    更新日期：1983-03-15 00:00:00

  • Structural consequences of cysteinylation of Cu/Zn-superoxide dismutase.

    abstract：:The metalloenzyme Cu/Zn-superoxide dismutase (SOD1) catalyzes the reduction of superoxide anions into molecular oxygen and hydrogen peroxide. Hydrogen peroxide can oxidize SOD1, resulting in aberrant protein conformational changes, disruption of SOD1 function, and DNA damage. Cells may have evolved mechanisms of regul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400613h

    authors： Auclair JR,Brodkin HR,D'Aquino JA,Petsko GA,Ringe D,Agar JN

    更新日期：2013-09-10 00:00:00

  • Modification of aldose reductase by S-nitrosoglutathione.

    abstract：:Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9714722

    authors： Chandra A,Srivastava S,Petrash JM,Bhatnagar A,Srivastava SK

    更新日期：1997-12-16 00:00:00

  • The 2.7 A crystal structure of the activated FERM domain of moesin: an analysis of structural changes on activation.

    abstract：:Moesin binds to a large range of proteins through its N terminal FERM (band 4.1, ezrin, radixin, moesin) domain. In full-length moesin isolated from cells, this binding is masked by binding to the C-terminal domain of moesin (C-ERMAD). Activation takes place by phosphorylation of Thr 558 in the C-ERMAD, which releases...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010419h

    authors： Edwards SD,Keep NH

    更新日期：2001-06-19 00:00:00

  • Analysis of the kinetics of ovine follitropin agonist-antagonist interactions with pig ovarian membranes.

    abstract：:The binding of 125I-labeled ovine follitropin (oFSH) and 125I-labeled deglycosylated ovine follitropin (DG-oFSH) to porcine granulosa cell membranes was studied at equilibrium and nonequilibrium binding conditions and statistically analyzed. Saturation and competition binding experiments revealed homogeneity in the po...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00386a059

    authors： Sebok K,De Lean A,Sairam MR

    更新日期：1987-06-16 00:00:00

  • Identification of peptide antagonists to glycoprotein Ibalpha that selectively inhibit von Willebrand factor dependent platelet aggregation.

    abstract：:GPIbalpha is an integral membrane protein of the GPIb-IX-V complex found on the platelet surface that interacts with the A1 domain of von Willebrand factor (vWF-A1). The interaction of GPIbalpha with vWF-A1 under conditions of high shear stress is the first step in platelet-driven thrombus formation. Phage display was...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702428q

    authors： Benard SA,Smith TM,Cunningham K,Jacob J,DeSilva T,Lin L,Shaw GD,Kriz R,Kelleher KS

    更新日期：2008-04-22 00:00:00

  • ATP-induced dimerization of the F0F1 ε subunit from Bacillus PS3: a hydrogen exchange-mass spectrometry study.

    abstract：:F0F1 ATP synthase harnesses a transmembrane electrochemical gradient for the production of ATP. When operated in reverse, this multiprotein complex catalyzes ATP hydrolysis. In bacteria, the ε subunit is involved in regulating this ATPase activity. Also, ε is essential for coupling ATP hydrolysis (or synthesis) to pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi5004684

    authors： Rodriguez AD,Dunn SD,Konermann L

    更新日期：2014-06-24 00:00:00

  • Comparison of N-glycosides of fetuins from different species and human alpha 2-HS-glycoprotein.

    abstract：:Complex type N-glycosides of commercial bovine fetuin preparations from pooled fetal calf serum have been shown to contain comparable amounts of Gal4,4,4TRI (see structure A below) and Gal4,4,3TRI (structure B) as major asialo-structures. To investigate whether there is a clear genetic specificity for synthesis of the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00135a024

    authors： Hayase T,Rice KG,Dziegielewska KM,Kuhlenschmidt M,Reilly T,Lee YC

    更新日期：1992-05-26 00:00:00

  • Characterization and stereochemistry of cofactor oxidation by a type II dihydrofolate reductase.

    abstract：:Type II dihydrofolate reductases (DHFRs) encoded by the R67 and R388 plasmids are different both in sequence and in structure from known chromosomal DHFRs. These plasmid-derived DHFRs are responsible for conferring trimethoprim resistance to the host strain. A derivative of R388 DHFR, RBG200, has been cloned and overp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00494a011

    authors： Brito RM,Reddick R,Bennett GN,Rudolph FB,Rosevear PR

    更新日期：1990-10-23 00:00:00

  • Recognition of cisplatin-DNA interstrand cross-links by replication protein A.

    abstract：:Replication protein A (RPA) is a heterotrimeric protein that is required for DNA replication and most DNA repair pathways. RPA has previously been shown to play a role in recognizing and binding damaged DNA during nucleotide excision repair (NER). RPA has also been suggested to play a role in psoralen DNA interstrand ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800460d

    authors： Patrick SM,Tillison K,Horn JM

    更新日期：2008-09-23 00:00:00

  • Sodium-dependent steps in the redox reactions of the Na+-motive NADH:quinone oxidoreductase from Vibrio harveyi.

    abstract：:The Na+-translocating NADH:ubiquinone oxidoreductase (Na+-NQR) from Vibrio harveyi was purified and studied by EPR and visible spectroscopy. Two EPR signals in the NADH-reduced enzyme were detected: one, a radical signal, and the other a line around g = 1.94, which is typical for a [2Fe-2S] cluster. An E(m) of -267 mV...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002545b

    authors： Bogachev AV,Bertsova YV,Barquera B,Verkhovsky MI

    更新日期：2001-06-19 00:00:00

  • Synthetic Riboswitches: From Plug and Pray toward Plug and Play.

    abstract：:In synthetic biology, metabolic engineering, and gene therapy, there is a strong demand for orthogonal or externally controlled regulation of gene expression. Here, RNA-based regulatory devices represent a promising emerging alternative to proteins, allowing a fast and direct control of gene expression, as no synthesi...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/acs.biochem.6b01218

    authors： Etzel M,Mörl M

    更新日期：2017-03-07 00:00:00

  • Molecular mechanism of action of β-hairpin antimicrobial peptide arenicin: oligomeric structure in dodecylphosphocholine micelles and pore formation in planar lipid bilayers.

    abstract：:The membrane-active, cationic, β-hairpin peptide, arenicin, isolated from marine polychaeta Arenicola marina exhibits a broad spectrum of antimicrobial activity. The peptide in aqueous solution adopts the significantly twisted β-hairpin conformation without pronounced amphipathicity. To assess the mechanism of arenici...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200746t

    authors： Shenkarev ZO,Balandin SV,Trunov KI,Paramonov AS,Sukhanov SV,Barsukov LI,Arseniev AS,Ovchinnikova TV

    更新日期：2011-07-19 00:00:00

  • Type II Binders Targeting the "GLR-Out" Conformation of the Pseudokinase STRADα.

    abstract：:Pseudokinases play important roles in signal transduction and cellular processes similar to those of catalytically competent kinases. However, pseudokinase pharmacological tractability and conformational space accessibility are poorly understood. Pseudokinases have only recently been suggested to adopt "inactive" conf...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00714

    authors： Smith RHB,Khan ZM,Ung PM,Scopton AP,Silber L,Mack SM,Real AM,Schlessinger A,Dar AC

    更新日期：2021-01-13 00:00:00

  • Photoaffinity labeling of the alpha 1-adrenergic receptor using an 125I-labeled aryl azide analogue of prazosin.

    abstract：:alpha 1-Adrenergic receptor probes, which can be radioiodinated to yield high specific activity radioligands, have been synthesized and characterized. 2-[4-(4-Amino-benzoyl)piperazin-1-yl]-4-amino-6,7-dimethoxyquin azoline (CP63,155), an arylamine analogue of the selective alpha 1-adrenergic antagonist prazosin, and i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00311a031

    authors： Seidman CE,Hess HJ,Homcy CJ,Graham RM

    更新日期：1984-07-31 00:00:00

  • A monosaccharide is bound to the sodium pump alpha-subunit.

    abstract：:We have recently reported that the Na pump alpha-subunit has cytosolic-oriented oligosaccharides which were sensitive to cleavage by an enzyme specific for hydrolysis of N-linked glycans [Pedemonte et al. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 9789-9793]. We now describe experiments that characterize the saccharides...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00158a008

    authors： Pedemonte CH,Kaplan JH

    更新日期：1992-11-03 00:00:00

  • Kinetic and secondary structure analysis of Naegleria andersoni GIR1, a group I ribozyme whose putative biological function is site-specific hydrolysis.

    abstract：:NanGIR1 is a catalytic element inserted in the P6 loop of a group I intron (NanGIR2) in the small subunit rRNA precursor of the protist Naegleria andersoni [Einvik, C., Decatur, W. A., Embley, T. M., Vogt, V. M., and Johansen, S. (1997) RNA 3, 710-720]. It catalyzes site-specific hydrolysis at an internal processing s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9718595

    authors： Jabri E,Aigner S,Cech TR

    更新日期：1997-12-23 00:00:00

  • The limits of promiscuity: isoform-specific dimerization of filamins.

    abstract：:Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026501+

    authors： Himmel M,Van Der Ven PF,Stöcklein W,Fürst DO

    更新日期：2003-01-21 00:00:00

  • Structural and dynamic aspects of binding of a prototype lexitropsin to the decadeoxyribonucleotide d(CGCAATTGCG)2 deduced from high-resolution 1H NMR Studies.

    abstract：:Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d(CGCAATTGCG)2 and the interaction between a prototype lexitropsin, or information-reading oligopeptide, and the decadeoxyribonucleotide are deduced by using high-resolution 1H NMR techniques. The nonexchangeable and imino proton reson...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00401a066

    authors： Lee M,Chang DK,Hartley JA,Pon RT,Krowicki K,Lown JW

    更新日期：1988-01-12 00:00:00

  • Sulfide binding properties of truncated hemoglobins.

    abstract：:The truncated hemoglobins from Bacillus subtilis (Bs-trHb) and Thermobifida fusca (Tf-trHb) have been shown to form high-affinity complexes with hydrogen sulfide in their ferric state. The recombinant proteins, as extracted from Escherichia coli cells after overexpression, are indeed partially saturated with sulfide, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901671d

    authors： Nicoletti FP,Comandini A,Bonamore A,Boechi L,Boubeta FM,Feis A,Smulevich G,Boffi A

    更新日期：2010-03-16 00:00:00

  • Aminoacyl-tRNA synthetases from yeast: generality of chemical proofreading in the prevention of misaminoacylation of tRNA.

    abstract：:The specificity of valyl-, phenylalanyl-, and tyrosyl-tRNA synthetases from yeast has been examined by a series of stringent tests designed to eliminate the possibility of artefactual interference. Valyl-tRNA synthetase, as well as activating a number of amino acid analogues, will accept alanine, cysteine, isoleucine,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00610a006

    authors： Igloi GL,von der Haar F,Cramer F

    更新日期：1978-08-22 00:00:00

  • Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived from Spirulina platensis Provides Important Insights into Intein-Mediated Catalysis of Protein Splicing.

    abstract：:Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a 136-residue DnaX mini-intein enzyme derived from the cyanobacterium Spirulina pla...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00828

    authors： Boral S,Maiti S,Basak AJ,Lee W,De S

    更新日期：2020-12-22 00:00:00

  • Equilibrium and stop-flow kinetic studies of fluorescently labeled DNA substrates with DNA repair proteins XPA and replication protein A.

    abstract：:Nucleotide excision repair (NER) is a crucial pathway in the maintenance of genome stability requiring at least two dozen proteins. XPA and RPA have essential roles in the damage recognition step of NER. To better understand the mechanism of their interactions with DNA, we utilized equilibrium and stop-flow kinetic ap...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011041q

    authors： Iakoucheva LM,Walker RK,van Houten B,Ackerman EJ

    更新日期：2002-01-08 00:00:00

  • Electrostatic effects in myoglobin. Application of the modified Tanford-Kirkwood theory to myoglobins from horse, California grey whale, harbor seal, and California sea lion.

    abstract：:The modified Tanford-Kirkwood electrostatic theory (Shire et al., 1974a) was applied to ferrimyoglobins from the following animal species: sperm whale (Physeter catodon), horse, California grey whale (Eschrichtius gibbosus), harbor seal (Phoca vitulina), and California sea lion (Zalophus californianus). Computations w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00678a002

    authors： Shire SJ,Hanania GI,Gurd FR

    更新日期：1975-04-08 00:00:00

  • Transthyretin mutation Leu-55-Pro significantly alters tetramer stability and increases amyloidogenicity.

    abstract：:A recently reported variant of human transthyretin (TTR), Leu-55-Pro, implicated as the causative agent in early-onset familial amyloid polyneuropathy was expressed and characterized, and its denaturation pathway and amyloidogenicity were compared to those of wild-type transthyretin. The overlap-extension polymerase c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00096a024

    authors： McCutchen SL,Colon W,Kelly JW

    更新日期：1993-11-16 00:00:00

  • Effects of pH and calcium ions on the conformational transitions in silk fibroin using 2D Raman correlation spectroscopy and 13C solid-state NMR.

    abstract：:Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the fac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049344i

    authors： Zhou P,Xie X,Knight DP,Zong XH,Deng F,Yao WH

    更新日期：2004-09-07 00:00:00

  • Specific stimulation of the T7 gene 6 exonuclease by the phage T7 coded deoxyribonucleic acid binding protein.

    abstract：:Bacteriophage T7 codes for a single-stranded DNA binding protein. This protein is the product of gene 2.5 and has been found previously to stimulate specifically the activity of the phage-coded DNA polymerase. We report here that the T7 DNA binding protein also stimulates the activity of the phage-coded exonuclease. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00266a042

    authors： Roberts L,Sadowski P,Wong JT

    更新日期：1982-11-09 00:00:00

  • Crystal structure of an ecotin-collagenase complex suggests a model for recognition and cleavage of the collagen triple helix.

    abstract：:The crystal structure of fiddler crab collagenase complexed with the dimeric serine protease inhibitor ecotin at 2.5 A resolution reveals an extended cleft providing binding sites for at least 11 contiguous substrate residues. Comparison of the positions of nine intermolecular main chain hydrogen bonding interactions ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9617522

    authors： Perona JJ,Tsu CA,Craik CS,Fletterick RJ

    更新日期：1997-05-06 00:00:00

  • Ligand-induced differences in secondary structure of the Vibrio parahaemolyticus Na+/galactose cotransporter.

    abstract：:A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi025692d

    authors： le Coutre J,Turk E,Kaback HR,Wright EM

    更新日期：2002-06-25 00:00:00

  • Cooperativity and oligomeric status of cardiac muscarinic cholinergic receptors.

    abstract：:Muscarinic cholinergic receptors can appear to be more numerous when labeled by [(3)H]quinuclidinylbenzilate (QNB) than by N-[(3)H]methylscopolamine (NMS). The nature of the implied heterogeneity has been studied with M(2) receptors in detergent-solubilized extracts of porcine atria. The relative capacity for [(3)H]NM...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011746s

    authors： Park PS,Sum CS,Pawagi AB,Wells JW

    更新日期：2002-04-30 00:00:00