Electrostatic effects in myoglobin. Application of the modified Tanford-Kirkwood theory to myoglobins from horse, California grey whale, harbor seal, and California sea lion.

abstract：:Imidazole glycerol phosphate synthase (ImGPS) from Thermotoga maritima is a model enzyme for studying allostery. The ImGPS complex consists of the cyclase subunit HisF and the glutaminase subunit HisH whose activity is stimulated by substrate binding to HisF in a V-type manner. To investigate the significance of a put...

journal_title：Biochemistry

authors： Kneuttinger AC,Rajendran C,Simeth NA,Bruckmann A,König B,Sterner R

更新日期：2020-07-28 00:00:00

abstract：:Bacillus subtilis CsoR (Bsu CsoR) is a copper-sensing transcriptional repressor that regulates the expression of the copZA operon encoding a copper chaperone and a Cu efflux P-type ATPase, respectively. Bsu CsoR is a homologue of Mycobacterium tuberculosis CsoR (Mtb CsoR), representative of a large Cu(I)-sensing regul...

journal_title：Biochemistry

authors： Ma Z,Cowart DM,Scott RA,Giedroc DP

更新日期：2009-04-21 00:00:00

abstract：:An antibody population that reacted with the high-affinity lysine binding site of human plasminogen was elicited by immunizing rabbits with an elastase degradation product containing kringles 1-3 (EDP I). This antibody was immunopurified by affinity chromatography on plasminogen-Sepharose and elution with 0.2 M 6-amin...

journal_title：Biochemistry

authors： Miles LA,Plow EF

更新日期：1986-11-04 00:00:00

abstract：:Site-directed mutagenesis studies have confirmed that the distal histidine in myoglobin stabilizes bound O2 by hydrogen bonding and have suggested that it is the polar character of the imidazole side chain rather than its size that limits the rate of ligand entry into the protein. We constructed an isosteric Val68 to ...

journal_title：Biochemistry

authors： Smerdon SJ,Dodson GG,Wilkinson AJ,Gibson QH,Blackmore RS

更新日期：1991-06-25 00:00:00

abstract：:Cerebrovascular deposition of fibrillar 39-42 amino acid amyloid beta-protein (Abeta), a condition known as cerebral amyloid angiopathy (CAA), is a key pathological feature of Alzheimer's disease and related disorders including hereditary cerebral hemorrhage with amyloidosis-Dutch type (HCHWA-D). Severe cases of CAA, ...

journal_title：Biochemistry

authors： Wagner MR,Keane DM,Melchor JP,Auspaker KR,Van Nostrand WE

更新日期：2000-06-27 00:00:00

abstract：:The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...

journal_title：Biochemistry

authors： Bermejo GA,Strub MP,Ho C,Tjandra N

更新日期：2010-03-09 00:00:00

abstract：:The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alky...

journal_title：Biochemistry

authors： Jo H,Culik RM,Korendovych IV,Degrado WF,Gai F

更新日期：2010-12-14 00:00:00

abstract：:The X-ray structures of neuronal nitric oxide synthase (nNOS) with N(omega)-hydroxy-l-arginine (l-NHA) and CO (or NO) bound have been determined at 1.91-2.2 A resolution. Microspectrophotometric techniques confirmed reduced redox state and the status of diatomic ligand complexes during X-ray diffraction data collectio...

journal_title：Biochemistry

authors： Doukov T,Li H,Soltis M,Poulos TL

更新日期：2009-11-03 00:00:00

abstract：:The extent of nativeness of the stable conformation of the thermolysin fragment containing the carboxyl-terminal third of the protein (from residues 206 to 316, denoted fragment FII) was examined by its immunogenic and antigenic characteristics. Antisera elicited in rabbits by either intact thermolysin or fragment FII...

journal_title：Biochemistry

authors： Vita C,Fontana A,Chaiken IM

更新日期：1982-04-27 00:00:00

abstract：:Band 3, the anion transport protein of human erythrocyte membranes, can be transferred from cells to liposomes and from liposomes back to cell membranes, retaining function and native orientation. After incubation with cells, sonicated phosphatidylcholine vesicles bind a transmembrane protein that comigrates with band...

journal_title：Biochemistry

authors： Newton AC,Cook SL,Huestis WH

更新日期：1983-12-20 00:00:00

abstract：:Crystals of the Y195F mutant of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 were subjected to a double soaking procedure, in which they were first soaked in a solution containing the inhibitor acarbose and subsequently in a solution containing maltohexaose. The refined structure of the resultin...

journal_title：Biochemistry

authors： Strokopytov B,Knegtel RM,Penninga D,Rozeboom HJ,Kalk KH,Dijkhuizen L,Dijkstra BW

更新日期：1996-04-02 00:00:00

abstract：:Mitochondria act as a focal point for upstream apoptosis signals by releasing cytochrome c into the cytosol, leading to the activation of caspases and subsequent cell death. Members of the Bcl-2 protein family regulate this phenomenon by heterodimerization via the BH3 domain of proapoptotic members opposing their pro-...

journal_title：Biochemistry

authors： Moradi-Améli M,Lorca T,Ficheux D,di Pietro A,Gillet G

更新日期：2002-07-09 00:00:00

abstract：:Recent crystallographic data suggest that conserved residues in the connection subdomain and C-terminal ribonuclease H (RNase H) domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template relative to the RNase H catalytic ce...

journal_title：Biochemistry

authors： Rausch JW,Lener D,Miller JT,Julias JG,Hughes SH,Le Grice SF

更新日期：2002-04-16 00:00:00

abstract：:Enzymes of halophilic organisms contain unusual peptide motifs that are absent from their mesophilic counterparts. The functions of these halophile-specific peptides are largely unknown. Here we have identified an unusual peptide that is unique to several halophile archaeal cysteinyl-tRNA synthetases (CysRS), which ca...

journal_title：Biochemistry

authors： Evilia C,Hou YM

更新日期：2006-06-06 00:00:00

abstract：:Incubation of 3-(2-phenylethyl)-4-methylsydnone (PMS) with liver microsomes from phenobarbital-pretreated rats or with reconstituted cytochrome P-450b results in loss of the enzyme chromophore. Chromophore loss is NADPH-dependent even though the sydnone decomposes by an oxygen- but not enzyme-dependent process to give...

journal_title：Biochemistry

authors： Grab LA,Swanson BA,Ortiz de Montellano PR

更新日期：1988-06-28 00:00:00

abstract：:The mannitol transport protein (EIImtl) carries out translocation with concomitant phosphorylation of mannitol from the periplasm to the cytoplasm, at the expense of phosphoenolpyruvate (PEP). The phosphoryl group which is needed for this group translocation is sequentially transferred from PEP via two phosphorylation...

journal_title：Biochemistry

authors： van Weeghel RP,van der Hoek YY,Pas HH,Elferink M,Keck W,Robillard GT

更新日期：1991-02-19 00:00:00

abstract：:The hallmark of serpins is the ability to undergo the so-called "stressed-to-relaxed" switch during which the surface-exposed reactive center loop (RCL) becomes incorporated as strand 4 in central beta-sheet A. RCL insertion drives not only the inhibitory reaction of serpins with their target serine proteases but also...

journal_title：Biochemistry

authors： Madsen JB,Dupont DM,Andersen TB,Nielsen AF,Sang L,Brix DM,Jensen JK,Broos T,Hendrickx ML,Christensen A,Kjems J,Andreasen PA

更新日期：2010-05-18 00:00:00

abstract：:Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge...

journal_title：Biochemistry

authors： Connell GJ,Illangesekare M,Yarus M

更新日期：1993-06-01 00:00:00

abstract：:Fibrinogen (approximately 10(-5) M) labilizes heterologous interactions within the thrombin-modified factor XIII zymogen (i.e., XIII' = a2'b2) so that, in the time frame (ca. 10 min) of normal clotting in plasma (37 degrees C, mu = 0.15, pH 7.5), 1.5 mM Ca2+ is sufficient to cause the release of the noncatalytic b sub...

journal_title：Biochemistry

authors： Credo RB,Curtis CG,Lorand L

更新日期：1981-06-23 00:00:00

abstract：:Aminoimidazole riobnucleotide carboxylase, the sixth step in the purine biosynthetic pathway, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to carboxyaminoimidazole ribonucleotide (CAIR). The gene products of the purE and purK genes (PurE and PurK, respectively) thought to be responsible for this act...

journal_title：Biochemistry

authors： Meyer E,Leonard NJ,Bhat B,Stubbe J,Smith JM

更新日期：1992-06-02 00:00:00

abstract：:Cell free protein synthesis (CFPS) has emerged as a promising methodology for protein expression. While polypeptide production is very reliable and efficient using CFPS, the correct cotranslational folding of membrane proteins during CFPS is still a challenge. In this contribution, we describe a two-step protocol in w...

journal_title：Biochemistry

authors： Focke PJ,Hein C,Hoffmann B,Matulef K,Bernhard F,Dötsch V,Valiyaveetil FI

更新日期：2016-08-02 00:00:00

abstract：:In order to further characterize the binding of the hybrid molecule NetOPC [bis(pyrrolecarboxamide)-oxazolopyridocarbazole conjugate] to double-stranded DNA, we have performed a molecular modeling study to investigate the binding modes of the complexes possibly formed between NetOPC and synthetic polynucleotides poly ...

journal_title：Biochemistry

authors： Goulaouic H,Carteau S,Subra F,Mouscadet JF,Auclair C,Sun JS

更新日期：1994-02-15 00:00:00

abstract：:Mild acid hydrolysis of Hafnia alvei strain 2 lipopolysaccharide released no O-specific polysaccharide but instead gave a monomeric octasaccharide repeating unit with N-acetylneuraminic acid as the reducing terminus. In addition, a dimer of the octasaccharide repeating unit, and also a decasaccharide composed of a fra...

journal_title：Biochemistry

authors： Gamian A,Romanowska E,Dabrowski U,Dabrowski J

更新日期：1991-05-21 00:00:00

abstract：:Fluorescence lifetimes and fluorescence anisotropy decays of the voltage-sensitive styryl-pyridinium dye RH421 have been measured in the presence of dimyristoylphosphatidylcholine vesicles, the water soluble enzyme ribulose 1,5-bisphosphate carboxylase/oxygenase (rubisco), and Na+,K(+)-ATPase-containing membrane fragm...

journal_title：Biochemistry

authors： Visser NV,van Hoek A,Visser AJ,Frank J,Apell HJ,Clarke RJ

更新日期：1995-09-19 00:00:00

abstract：:Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...

journal_title：Biochemistry

authors： Mills M,Völker J,Klump HH

更新日期：1996-10-15 00:00:00

abstract：:We have studied a group of fusion peptides of influenza hemagglutinin in which the N-terminal amino acid, Gly (found in the wild-type peptide), has been systematically substituted with Ala, Ser, Val, or Glu. The activity of the intact hemagglutinin protein with these same substitutions has already been reported. As a ...

journal_title：Biochemistry

authors： Epand RM,Epand RF,Martin I,Ruysschaert JM

更新日期：2001-07-31 00:00:00

abstract：:Previous studies have shown that the short-motif electroplax Na channel is phosphorylated in vitro by cyclic AMP-dependent protein kinase (PKA) at serines 6 or 7 and 1776 and threonine 17 (Emerick & Agnew, 1989). We here show that phosphatase treatment of solubilized, purified Na channels enhanced subsequent PKA label...

journal_title：Biochemistry

authors： Emerick MC,Shenkel S,Agnew WS

更新日期：1993-09-14 00:00:00

abstract：:Fluorometric titrations of papain, succinyl-papain, and the corresponding methylthio derivatives of Cys-25 (papain-S-SCH3 and succinyl-papain-S-SCH3) were determined. Removal of the methylthio group from Cys-25 resulted in an increase of approximately 4 pK units in the fluorometrically determined pK value. The corresp...

journal_title：Biochemistry

authors： Johnson FA,Lewis SD,Shafer JA

更新日期：1981-01-06 00:00:00

abstract：:Native EcoRI DNA methyltransferase (Mtase, Mr 38,050) is proteolyzed by trypsin to generate an intermediate 36-kDa fragment (p36) followed by the formation of two polypeptides of Mr 23,000 and 13,000 (p23 and p13, respectively). Protein sequence analysis of the tryptic fragments indicates that p36 results from removal...

journal_title：Biochemistry

authors： Reich NO,Maegley KA,Shoemaker DD,Everett E

更新日期：1991-03-19 00:00:00

abstract：:Excluded volume and viscosity effects of crowding agents that mimic crowded conditions in vivo on "classical" burst phase folding kinetics of cytochrome c are assessed in vitro. Upon electron transfer-triggered folding of reduced cytochrome c, far-UV time-resolved circular dichroism (TRCD) is used to monitor folding u...

journal_title：Biochemistry

authors： Chen E,Christiansen A,Wang Q,Cheung MS,Kliger DS,Wittung-Stafshede P

更新日期：2012-12-11 00:00:00