The 2.7 A crystal structure of the activated FERM domain of moesin: an analysis of structural changes on activation.

abstract：:This work describes the biochemical characterization of the catalytic domain of Ira2p, a Saccharomyces cerevisiae GTPase-activating protein (GAP) regulating the RAS gene products. A fragment of 383 residues (amino acids 1644-2026) was produced in Escherichia coli as glutathione S-transferase fusion protein (GST-Ira2p-...

journal_title：Biochemistry

authors： Parrini MC,Jacquet E,Bernardi A,Jacquet M,Parmeggiani A

更新日期：1995-10-24 00:00:00

abstract：:Time-resolved spectroscopic studies of unfolded horse iron(II) cytochrome c have suggested that the imidazole side chains of His26 and His33 bind transiently to the heme iron on microsecond time scales, after photodissociation of a carbon monoxide ligand from the heme. Our studies of four variants of cytochrome c (hor...

journal_title：Biochemistry

authors： Hagen SJ,Latypov RF,Dolgikh DA,Roder H

更新日期：2002-01-29 00:00:00

abstract：:Positively charged cyclic hexapeptide Phe-Arg-Cys-Arg-Cys-Phe-CONH2 (FRCRCFa) represents a group of conformationally constrained peptides that block the cardiac sarcolemma Na(+)-Ca2+ exchanger [Khananshvili et al. (1995b) J. Biol. Chem. 270, 16182-16188]. Here, we study the kinetic mechanisms of FRCRCFa-induced inhibi...

journal_title：Biochemistry

authors： Khananshvili D,Baazov D,Weil-Maslansky E,Shaulov G,Mester B

更新日期：1996-12-10 00:00:00

abstract：:Recent studies of proteins with reversed charged residues have demonstrated that electrostatic interactions on the surface can contribute significantly to protein stability. We have used the approach of reversing negatively charged residues using Arg to evaluate the effect of the electrostatics context on the transiti...

journal_title：Biochemistry

authors： Mancusso R,Cruz E,Cataldi M,Mendoza C,Fuchs J,Wang H,Yang X,Tasayco ML

更新日期：2004-04-06 00:00:00

abstract：:In iron-deficient conditions, Pseudomonas aeruginosa secretes a major fluorescent siderophore named pyoverdin (Pvd), which after chelating iron(III) is transported back into the cell via its outer membrane receptor FpvA. FpvA is a TonB-dependent transport protein and has the ability to bind Pvd in its apo- or iron-loa...

journal_title：Biochemistry

authors： Clément E,Mesini PJ,Pattus F,Schalk IJ

更新日期：2004-06-22 00:00:00

abstract：:The flash-induced Fourier transform infrared (FTIR) difference spectrum of the oxygen-evolving Mn cluster upon S1-to-S2 transition (S2/S1 spectrum) was measured using photosystem II (PS II) core complexes of Synechocystis 6803 in which tyrosine residues were specifically labeled with 13C at the ring-4 position. The do...

journal_title：Biochemistry

authors： Noguchi T,Inoue Y,Tang XS

更新日期：1997-12-02 00:00:00

abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...

journal_title：Biochemistry

authors： Maddukuri L,Shuck SC,Eoff RL,Zhao L,Rizzo CJ,Guengerich FP,Marnett LJ

更新日期：2013-12-03 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:Cyanide inhibits the CO oxidation activity of carbon monoxide dehydrogenase from Clostridium thermoaceticum by binding tightly to the form of the C-cluster yielding the gav = 1.82 signal (the C1.82 form). CN- dissociates and the enzyme reactivates upon addition of CO, CO2 plus dithionite, or CS2 plus dithionite. Dithi...

journal_title：Biochemistry

authors： Anderson ME,Lindahl PA

更新日期：1994-07-26 00:00:00

abstract：:The binding of horse ferricytochrome c to yeast cytochrome c peroxidase at pH 6.0 in 8.7 mM phosphate buffer (0.0100 M ionic strength) is characterized by a small, unfavorable enthalpy change (+1.91 +/- 0.16 kcal mol-1) and a large, positive entropy change (+37 +/- 1 eu). The free energy of binding depends strongly up...

journal_title：Biochemistry

authors： Kresheck GC,Vitello LB,Erman JE

更新日期：1995-07-04 00:00:00

abstract：:Phenylalanine 87 of Bacillus megaterium cytochrome P450 BM3, a residue close to the heme in the substrate binding pocket, has been replaced by alanine by site-directed mutagenesis. The substitution had no effect on the rate of hydroxylation of laurate and increased the affinity for laurate of both the intact enzyme an...

journal_title：Biochemistry

authors： Oliver CF,Modi S,Sutcliffe MJ,Primrose WU,Lian LY,Roberts GC

更新日期：1997-02-18 00:00:00

abstract：:1H NMR spectroscopy of the isotropically shifted signals in cobalt carboxypeptidase, CoCPD, permits a direct and selective detection of protons belonging to the residues liganded to the metal. The chemical shift of these protons in the free enzyme and enzyme-inhibitor complexes with changing pH monitors the state of i...

journal_title：Biochemistry

authors： Auld DS,Bertini I,Donaire A,Messori L,Moratal JM

更新日期：1992-04-21 00:00:00

abstract：:Transferrins have been extensively studied in order to understand how they reversibly bind and release iron. Human serum transferrin (hTF) is a single polypeptide chain that folds into two lobes (N- and C-lobe); each lobe binds a single ferric ion. Iron release induces a large conformational change in each lobe. At th...

journal_title：Biochemistry

authors： James NG,Berger CL,Byrne SL,Smith VC,MacGillivray RT,Mason AB

更新日期：2007-09-18 00:00:00

abstract：:We have investigated effects of chemical modifications of a third strand on the thermodynamic and kinetic properties of the triplex formation between a 23-bp duplex and each of four kinds of 15-mer chemically modified third strands using isothermal titration calorimetry and interaction analysis system. The chemical mo...

journal_title：Biochemistry

authors： Torigoe H,Shimizume R,Sarai A,Shindo H

更新日期：1999-11-02 00:00:00

abstract：:In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...

journal_title：Biochemistry

authors： Stein RL,Melandri F,Dick L

更新日期：1996-04-02 00:00:00

abstract：:Factor XII was purified approximately 14 000-fold from bovine plasma by ammonium sulfate fractionation followed by heparin-agarose, DEAE-Sephadex, CM-cellulose, arginine-agarose, and benzamidine-agarose column chromatography. By this method, about 15 mg of protein was purified from 15 L of plasma with an overall yield...

journal_title：Biochemistry

authors： Fujikawa K,Walsh KA,Davie EW

更新日期：1977-05-17 00:00:00

abstract：:The quantitative tissue specificity of the high mobility group (HMG) chromosomal proteins was investigated. Perchloric acid (PCA) extracts of four different chicken tissues and erythrocytes contained three proteins which comigrated on NaDodSO4-polyacrylamide gels with the HMG's 1,2, and E from erythrocyte nuclei. Thes...

journal_title：Biochemistry

authors： Gordon JS,Rosenfeld BI,Kaufman R,Williams DL

更新日期：1980-09-16 00:00:00

abstract：:A considerable body of data supports the model that the infectious agent (called a prion) which causes the transmissible spongiform encephalopathies is a replicating polypeptide devoid of nucleic acid. Prions are believed to propagate by changing the conformation of the normal cellular prion protein (PrPc) into an inf...

journal_title：Biochemistry

authors： Cappai R,Stewart L,Jobling MF,Thyer JM,White AR,Beyreuther K,Collins SJ,Masters CL,Barrow CJ

更新日期：1999-03-16 00:00:00

abstract：:The structural basis for the improvement in catalytic efficiency of the mutant E165D chicken triosephosphate isomerase by the secondary mutation, S96P, has been analyzed using a combination of X-ray crystallography and Fourier transform infrared spectroscopy. All X-ray structures were of the complex of phosphoglycoloh...

journal_title：Biochemistry

authors： Komives EA,Lougheed JC,Liu K,Sugio S,Zhang Z,Petsko GA,Ringe D

更新日期：1995-10-17 00:00:00

abstract：:A 37 kDa protein that binds to diadenosine tetraphosphate (Ap4A) was purified from human HeLa cells and identified as uracil DNA glycosylase/glyceraldehyde-3-phosphate dehydrogenase (UDG/GAPDH). Utilizing photoaffinity labeling with [alpha-32P]8N3-Ap4A, an Ap4A binding protein of 37 kDa was identified from HeLa cell n...

journal_title：Biochemistry

authors： Baxi MD,Vishwanatha JK

更新日期：1995-08-01 00:00:00

abstract：:The interactions of Pr(III) and Eu(III) with specifically nitrated derivatives of the basic bovine pancreatic trypsin inhibitor have been studied using optical spectroscopy and nuclear magnetic resonance (NMR) at 250 and 270 MHz. Stability constants for proton and metal binding to nitrotyrosines 10 and 21 determined o...

journal_title：Biochemistry

authors： Marinetti TD,Snyder GH,Sykes BD

更新日期：1977-02-22 00:00:00

abstract：:Human immunodeficiency virus type 1 protease (HIV-1 PR) is an essential enzyme in the HIV-1 life cycle. As such, this protein represents a major drug target in AIDS therapy, but emerging resistance to antiretroviral inhibitor cocktails, caused by high viral mutation rates, represents a significant challenge in AIDS tr...

journal_title：Biochemistry

authors： Genoni A,Morra G,Merz KM Jr,Colombo G

更新日期：2010-05-18 00:00:00

abstract：:Deoxycytidine kinase (dCK) uses either ATP or UTP as a phosphoryl donor to catalyze the phosphorylation of nucleoside acceptors. The kinetic properties of human dCK are modulated in vivo by phosphorylation of serine 74. This residue is a part of the insert region and is distant from the active site. Replacing the seri...

journal_title：Biochemistry

authors： Hazra S,Szewczak A,Ort S,Konrad M,Lavie A

更新日期：2011-04-12 00:00:00

abstract：:Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...

journal_title：Biochemistry

authors： Seeholzer SH,Jaworowski A,Rose IA

更新日期：1991-01-22 00:00:00

abstract：:Enzyme-substrate contacts in the hydrolysis of ester substrates by the cysteine protease papain were investigated by systematically altering backbone hydrogen-bonding and side-chain hydrophobic contacts in the substrate and determining each substrate's kinetic constants. The observed specificity energies [defined as d...

journal_title：Biochemistry

authors： Berti PJ,Faerman CH,Storer AC

更新日期：1991-02-05 00:00:00

abstract：:Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. W...

journal_title：Biochemistry

authors： Kamashev D,Oberto J,Serebryakova M,Gorbachev A,Zhukova Y,Levitskii S,Mazur AK,Govorun V

更新日期：2011-10-11 00:00:00

abstract：:NMR spectroscopy has been used to study the interaction of aminoglycoside antibiotics with an aminoglycoside antibiotic 3'-phosphotransferase [APH(3')-IIIa]. APH(3')-IIIa is an enterococcal enzyme that is responsible for the ATP-dependent O-phosphorylation of a broad range of aminoglycoside antibiotics. The NMR method...

journal_title：Biochemistry

authors： Cox JR,Serpersu EH

更新日期：1997-03-04 00:00:00

abstract：:A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein...

journal_title：Biochemistry

authors： Van Heeke G,Deforce L,Schnizer RA,Shaw R,Couton JM,Shaw G,Song PS,Schuster SM

更新日期：1993-09-28 00:00:00

abstract：:We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), ...

journal_title：Biochemistry

authors： Thornton K,Gorenstein DG

更新日期：1994-03-29 00:00:00

abstract：:When they are translated, steroid receptors are assembled into a multiprotein complex containing hsp90, p23, an immunophilin, and often some hsp70. Some of the receptors, such as that for progesterone, have nuclear localization signals that are functional in the absence of hormone, and they move into the nucleus where...

journal_title：Biochemistry

authors： Czar MJ,Galigniana MD,Silverstein AM,Pratt WB

更新日期：1997-06-24 00:00:00