Abstract：
:We have used 2D 1H NMR to determine the structure of glucagon-like peptide-1-(7-36) amide bound to a dodecylphosphocholine micelle. In this membranelike environment, the peptide hormone is shown to have a structure similar to that observed for glucagon. It consists of an N-terminal random coil segment (residues 1-7), two helical segments (7-14 and 18-29), and a linker region (15-17). The C-terminal helix is more stable than the N-helix as determined by amide proton exchange experiments. The C-terminal helix shows much larger alpha and amide proton upfield secondary shifts relative expected for a random coil conformation. This suggests a highly helical structure in this portion of the molecule. The C-terminal helix also has a much larger fraction of residues that are hydrophobic, presumably enhancing the interaction of this portion of the peptide with the micelle (or membrane). The structure refined from the NOESY data is not a uniform alpha-helix throughout residues 6-30. A uniform helix would not be perfectly amphiphilic since the hydrophobic face of the N-terminal portion of the helix is positioned in nearly perfect opposition to the hydrophobic face of the C-terminal portion. However, helical distortion around residues 15-17 allows a phase shift of the two helical segments to position nearly all of the hydrophobic residues (and none of the hydrophilic ones) on a single face of the distorted single helix as would be required to favorably interact with the hydrophobic portion of the micelle or membrane.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Thornton K,Gorenstein DGdoi
10.1021/bi00178a009subject
Has Abstractpub_date
1994-03-29 00:00:00pages
3532-9issue
12eissn
0006-2960issn
1520-4995journal_volume
33pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Conventional isoforms of protein kinase C (PKC) are activated when their two membrane-targeting modules, the C1 and C2 domains, bind the second messengers diacylglycerol (DG) and Ca2+, respectively. This study investigates the mechanism of Ca2+-induced binding of PKC betaII to anionic membranes mediated by the C2 doma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010761u
更新日期：2001-11-06 00:00:00
abstract：:Zinc, the catalytically essential metal of angiotensin converting enzyme (ACE), has been replaced by cobalt(II) to give an active, chromophoric enzyme that is spectroscopically responsive to inhibitor binding. Visible absorption spectroscopy and magnetic circular dichroic spectropolarimetry have been used to character...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00397a014
更新日期：1987-11-17 00:00:00
abstract：:Pulmonary surfactant protein D (SP-D), a member of the collectin group of innate immune proteins, plays important roles in lipopolysaccharide (LPS) recognition. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with Toll-like receptor (TLR) 2, resulting in alteration of TLR2-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060176z
更新日期：2006-07-18 00:00:00
abstract：:The interaction of myristoyllysophosphatidylcholine with bovine myelin basic protein at pH 7.4 and 4.5, I = 0.48, has been investigated by a recycling partition equilibrium technique with Bio-Gel P-2 as the gel phase. Important points to emerge from this direct binding study are that it is a monomeric (not micellar) a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00378a002
更新日期：1987-02-24 00:00:00
abstract：:The beta-glycosidase from the hyperthermophilic Archaeon Sulfolobus solfataricus hydrolyzes beta-glycosides following a retaining mechanism based upon the action of two amino acids: Glu387, which acts as the nucleophile of the reaction, and Glu206, which acts as the general acid/base catalyst. The activities of inacti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981855f
更新日期：1998-12-08 00:00:00
abstract：:A human cDNA, UDPGTh-3, encoding a dihydrotestosterone/5 alpha-androstane-3 alpha,17 beta-diol UDP- glucuronosyltransferase (transferase) has been isolated and characterized. The nucleotide sequence of UDPGTh-3 encodes a 530 amino acid protein with a typical membrane insertion-signal peptide, a membrane-anchoring doma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00091a015
更新日期：1993-10-12 00:00:00
abstract：:The innate immune response to infection or injury involves an antigen-antibody triggered classical pathway (CP) of complement activation, in which soluble and cell surface plasma proteins cooperatively effect elimination of foreign organisms and damaged host cells. However, protracted or dysfunctional complement activ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900679r
更新日期：2009-09-08 00:00:00
abstract：:The three catalytic sites of the F(O)F(1) ATP synthase interact through a cooperative mechanism that is required for the promotion of catalysis. Replacement of the conserved alpha subunit Arg-376 in the Escherichia coli F(1) catalytic site with Ala or Lys resulted in turnover rates of ATP hydrolysis that were 2 x 10(3...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992530h
更新日期：2000-03-14 00:00:00
abstract：:The interaction of a nitroxide spin-labeled derivative of ouabain with sheep kidney Na,K-ATPase and the motional behavior of the ouabain spin label-Na,K-ATPase complex have been studied by means of electron paramagnetic resonance (EPR) and saturation-transfer EPR (ST-EPR). Spin-labeled ouabain binds with high affinity...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00122a019
更新日期：1992-02-25 00:00:00
abstract：:Ryanodine receptor (RyR) mutations linked with some congenital skeletal and cardiac diseases are localized to three easily definable regions: region 1 (N-terminal domain), region 2 (central domain), and a rather broad region 3 containing the channel pore. As shown in our recent studies, the interdomain interaction bet...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061557f
更新日期：2007-04-10 00:00:00
abstract：:Oligomerization of numerous G protein-coupled receptors has been documented, including the prototypic family B secretin receptor. The clinical significance of oligomerization of this receptor became clear with the recent observation that a misspliced form present in pancreatic cancer could associate with the wild-type...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060494y
更新日期：2006-07-11 00:00:00
abstract：:The maltodextrin binding protein from Escherichia coli serves as the initial receptor for both the active transport of and chemotaxis toward a range of linear maltose sugars. The X-ray structures of both the maltose-bound and sugar-free forms of the protein have been previously described [Spurlino, J. C., Lu, G.-Y., &...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00091a004
更新日期：1993-10-12 00:00:00
abstract：:Oxidation of low-density lipoprotein (LDL), the major cholesterol carrier in plasma, is thought to promote atherogenesis via several mechanisms. One proposed mechanism involves fusion of oxidized LDL in the arterial wall; another involves oxidation-induced amyloid formation by LDL apolipoprotein B. To test these mecha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700225a
更新日期：2007-05-15 00:00:00
abstract：:The interaction of profilin and non-muscle beta,gamma-actin prepared from bovine spleen has been investigated under physiologic ionic conditions. Profilin binding to actin decreases the affinity of actin for MgADP and MgATP by about 65- and 13-fold, respectively. Kinetic measurements indicate that profilin binding to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001520+
更新日期：2000-10-31 00:00:00
abstract：:Histone tails in nucleosomes play critical roles in regulation of many biological processes, including chromatin compaction, transcription, and DNA repair. Moreover, post-translational modifications, notably lysine acetylation, are crucial to these functions. While the tails have been intensively studied, how the stru...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01208
更新日期：2017-04-11 00:00:00
abstract：:SecA-lipid interactions are believed to be important for the translocation of precursor proteins across the inner membrane of Escherichia coli [Lill, R., Dowhan, W., & Wickner, W. (1990) Cell 60, 271-280]. SecA insertion into the phospholipid bilayer could a role in this process. We investigated this possibility by st...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00119a021
更新日期：1992-02-04 00:00:00
abstract：:Two dioxygenases (ARD and ARD') were cloned from Klebsiella pneumoniae that catalyze different oxidative decomposition reactions of an advanced aci-reductone intermediate, CH(3)SCH(2)CH(2)COCH(OH)=CH(OH) (I), in the methionine salvage pathway. The two enzymes are remarkable in that they have the same polypeptide seque...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010110y
更新日期：2001-05-29 00:00:00
abstract：:Evidence of nonequilibrium local heating in transient spectra of LHCII, the main light-harvesting complex of plants, was studied by using various excitation intensities over a wide temperature range, from 10 K to room temperature. No obvious manifestation of local heating was found at room temperature, whereas at 10 K...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060048a
更新日期：2006-08-08 00:00:00
abstract：:A cationic, copper(II)-containing ligand, derived from bulky 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)porphyrin, Cu(T4), and two sterically friendlier forms, [trans-5,15-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(tD4), and [cis-5,10-di(N-methylpyridinium-4-yl)porphyrinato]copper(II), Cu(cD4), bind to DN...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi300828z
更新日期：2012-09-25 00:00:00
abstract：:Neuraminidase is one of the two glycoprotein spikes protruding from the influenza virus membrane. We have determined by X-ray crystallography the native structure of B/Lee/40 neuraminidase (NA) and the structures of its crystals soaked with a substrate, N-acetylneuraminyllactose (NANL), and an inhibitor, 2-deoxy-2,3-d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00193a002
更新日期：1994-07-12 00:00:00
abstract：:Fluoroaluminate and fluoroberyllate are potent inhibitors of the ATPase activity of myosin. Inhibition requires the presence of ADP, and much evidence has accumulated to suggest that the tetrahedral fluoroaluminate and fluoroberyllate ions act as phosphate analogues, binding with high affinity at the active site in th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00090a022
更新日期：1993-10-05 00:00:00
abstract：:The transformation of DMPC vesicles containing myristic acid to larger structures was examined by nuclear magnetic resonance techniques. The process was found to be characterized by a rate which is approximately first order in vesicle concentration. Rates, however, show a dramatic increase with increasing myristic aci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00610a027
更新日期：1978-08-22 00:00:00
abstract：:The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962219p
更新日期：1997-03-18 00:00:00
abstract：:Bleeding, the most serious complication of thrombolytic therapy with tissue-type plasminogen activator (t-PA), is thought to result from lysis of fibrin in hemostatic plugs and from the systemic lytic state caused by unopposed plasmin. One mechanism by which systemic plasmin can impair hemostasis is by partially degra...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0525730
更新日期：2006-04-04 00:00:00
abstract：:Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b01174
更新日期：2017-08-08 00:00:00
abstract：:The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991888a
更新日期：1999-12-21 00:00:00
abstract：:The deacetylation of UDP-3-O-[(R)-3-hydroxymyristoyl]-N-acetylglucosamine (UDP-3-O-acyl-GlcNAc) by LpxC is the committed reaction of lipid A biosynthesis. CHIR-090, a novel N-aroyl-l-threonine hydroxamic acid, is a potent, slow, tight-binding inhibitor of the LpxC deacetylase from the hyperthermophile Aquifex aeolicus...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi6025165
更新日期：2007-03-27 00:00:00
abstract：:In aqueous glycerol solutions, the rate constant for the formation of compound I of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. However, whem m-chloroperbenzoic acid is used to form compound I, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. Th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00632a023
更新日期：1977-06-28 00:00:00
abstract：:The mature form of subtilisin is an unusual example of a monomeric protein with a high kinetic barrier to folding and unfolding. Using site-directed mutagenesis of subtilisin BPN', we are attempting to determine the physical and energetic nature of the kinetic barrier. The high-affinity calcium-binding site A has been...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00090a012
更新日期：1993-10-05 00:00:00
abstract：:Transcarboxylase is made up of a central hexameric subunit (S20,W similar 12 S), three peripheral dimeric metallo subunits (S20,W similar to 5 S), and six biotinyl carboxyl carrier subunits (S20,W similar to 1.3 S). The results presented here show that the carboxyl carrier subunit is required for assembly of the 12S a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00679a010
更新日期：1975-04-22 00:00:00
