Peptide probe study of the role of interaction between the cytoplasmic and transmembrane domains of the ryanodine receptor in the channel regulation mechanism.

abstract：:Human transcriptional coactivator P/CAF (p300/CBP-associating factor) is a histone acetyltransferase (HAT) and is a member of the GNAT (GCN5 related N-acetyltransferases) superfamily. P/CAF was originally identified by its ability to activate transcription of a variety of genes through its interaction with p300/CBP. T...

journal_title：Biochemistry

authors： Tanner KG,Langer MR,Denu JM

更新日期：2000-10-03 00:00:00

abstract：:Human placental lipocortin is a high-affinity substrate for rat brain protein kinase C in vitro with phosphorylation occurring on serine and threonine residues in a ratio of approximately 2 to 1. Comparison of the ability of various N-terminal-truncated derivatives of lipocortin to serve as phosphorylation substrates,...

journal_title：Biochemistry

authors： Schlaepfer DD,Haigler HT

更新日期：1988-06-14 00:00:00

abstract：:Kinetic and structural changes in recombinant human aldose reductase (AR) due to modification by S-nitrosoglutathione (GSNO) were investigated. Incubation of the enzyme with 10-50 microM GSNO led to a time- and concentration-dependent inactivation of the enzyme, with a second-order rate constant of 0.087 +/- 0.009 M-1...

journal_title：Biochemistry

authors： Chandra A,Srivastava S,Petrash JM,Bhatnagar A,Srivastava SK

更新日期：1997-12-16 00:00:00

abstract：:A positional-scanning combinatorial protocol was employed to optimize the deoxyribose-based cleavage of B-form DNA by Ni(II) x Xaa-Xaa-His metallopeptides. This procedure employed 18 naturally occurring amino acids (excluding Cys and Trp) to generate two libraries in which the first and second positions of the peptide...

journal_title：Biochemistry

authors： Huang X,Pieczko ME,Long EC

更新日期：1999-02-16 00:00:00

abstract：:Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

journal_title：Biochemistry

authors： Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

更新日期：2004-02-03 00:00:00

abstract：:Site-specifically modified oligodeoxynucleotides were used to explore the influence of neighboring base sequence context on the mutagenic potential of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-AAF) and N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-AF) in mammalian cells. Oligodeoxynucleotides ((5)(')TCCTCCTNXNCTCT...

journal_title：Biochemistry

authors： Shibutani S,Suzuki N,Tan X,Johnson F,Grollman AP

更新日期：2001-03-27 00:00:00

abstract：:O-Aryloxycarbonyl hydroxamates have previously been shown to efficiently inactivate class C β-lactamases by cross-linking serine and lysine residues in the active site. A new analogue of these inhibitors, D-(R)-O-(phenoxycarbonyl)-N-[(4-amino-4-carboxy-1-butyl)oxycarbonyl]hydroxylamine, designed to inactivate certain ...

journal_title：Biochemistry

authors： Tilvawala R,Cammarata M,Adediran SA,Brodbelt JS,Pratt RF

更新日期：2015-12-22 00:00:00

abstract：:The interaction of the recombinant hemoglobin from Vitreoscilla sp. (VHb) with the bacterial membrane of Escherichia coli cells has been investigated by measuring the propensity of VHb to interact with monolayers formed by natural bacterial phosholipids. The measurements showed that the protein is capable of penetrati...

journal_title：Biochemistry

authors： Rinaldi AC,Bonamore A,Macone A,Boffi A,Bozzi A,Di Giulio A

更新日期：2006-04-04 00:00:00

abstract：:myo-Inositol (mI) is widely distributed in all domains of life and is important for several cellular functions, including bacterial survival. The enzymes responsible for the bacterial catabolism of mI, encoded in the iol operon, can vary from one organism to another, and these pathways have yet to be fully characteriz...

journal_title：Biochemistry

authors： Ramos-Figueroa JS,Aamudalapalli HB,Jagdhane RC,Smith J,Palmer DRJ

更新日期：2020-08-18 00:00:00

abstract：:Pyrrolocytosine (PC) and 2-aminopurine (2AP) are fluorescent nucleobase analogues of the DNA nucleobases cytosine and adenine, respectively, and form base pairs with guanine and thymine. Both fluorescent nucleobases are used extensively as probes for local structure in nucleic acids as the fluorescence properties of P...

journal_title：Biochemistry

authors： Bull GD,Thompson KC

更新日期：2018-07-31 00:00:00

abstract：:Candida albicans drug resistance protein 1 (Cdr1p), an ATP-dependent drug efflux pump, contributes to multidrug resistance in Candida-infected immunocompromised patients. Previous cell-based assays suggested that Cdr1p also acts as a phospholipid translocator. To investigate this, we reconstituted purified Cdr1p into ...

journal_title：Biochemistry

authors： Shukla S,Rai V,Saini P,Banerjee D,Menon AK,Prasad R

更新日期：2007-10-30 00:00:00

abstract：:β-Lactamase inhibitors (BLIs) restore the efficacy of otherwise obsolete β-lactams. However, commercially available BLIs are not effective against metallo-β-lactamases (MBLs), which continue to be disseminated globally. One group of the most clinically important MBLs is the VIM family. The discovery of VIM-24, a natur...

journal_title：Biochemistry

authors： Mojica MF,Mahler SG,Bethel CR,Taracila MA,Kosmopoulou M,Papp-Wallace KM,Llarrull LI,Wilson BM,Marshall SH,Wallace CJ,Villegas MV,Harris ME,Vila AJ,Spencer J,Bonomo RA

更新日期：2015-05-26 00:00:00

abstract：:The gene for human pancreatic cholesterol esterase consists of 11 exons and 10 introns and is 9.2 kb in length. The last and longest exon (841 nucleotides) is unique to the human gene. Functional amino acids are encoded on separate exons. The leader sequence is encoded by a single exon which carries two additional N-t...

journal_title：Biochemistry

authors： Kumar BV,Aleman-Gomez JA,Colwell N,Lopez-Candales A,Bosner MS,Spilburg CA,Lowe M,Lange LG

更新日期：1992-07-07 00:00:00

abstract：:The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either th...

journal_title：Biochemistry

authors： Morgan TR,Shand JA,Clarke SM,Eaton-Rye JJ

更新日期：1998-10-13 00:00:00

abstract：:Polyphosphate glucokinase from Mycobacterium tuberculosis catalyzes the phosphorylation of glucose using inorganic polyphosphates [poly(P)] or ATP. The steady-state kinetic mechanisms of the poly(P)- and ATP-dependent glucokinase reactions were investigated using initial velocity, product inhibition, and dead-end inhi...

journal_title：Biochemistry

authors： Hsieh PC,Kowalczyk TH,Phillips NF

更新日期：1996-07-30 00:00:00

abstract：:The synthesis of activated sulfate (adenosine 5'-phosphosulfate, APS) and inorganic pyrophosphate from ATP and SO4 is remarkably unfavorable: K(eq) approximately 10(-8) under presumed, near-physiological conditions. Consequently, ATP sulfurylases, which catalyze APS synthesis, suffer approximately 10(8)-fold losses in...

journal_title：Biochemistry

authors： Sun M,Leyh TS

更新日期：2006-09-26 00:00:00

abstract：:We investigated the molecular mechanisms and the binding site location for the fluorophor crystal violet (CrV), a noncompetitive antagonist of the nicotinic acetylcholine receptor (AChR). To this end, radiolabeled competition binding, fluorescence spectroscopy, Schild-type analysis, patch-clamp recordings, and molecul...

journal_title：Biochemistry

authors： Arias HR,Bhumireddy P,Spitzmaul G,Trudell JR,Bouzat C

更新日期：2006-02-21 00:00:00

abstract：:Thermodynamic and structural evidence indicates that the DNA binding domains of lac repressor (lacI) exhibit significant conformational adaptability in operator binding, and that the marginally stable helix-turn-helix (HTH) recognition element is greatly stabilized by operator binding. Here we use circular dichroism a...

journal_title：Biochemistry

authors： Felitsky DJ,Record MT Jr

更新日期：2003-02-25 00:00:00

abstract：:The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression ...

journal_title：Biochemistry

authors： Rohweder B,Lehmann G,Eichner N,Polen T,Rajendran C,Ruperti F,Linde M,Treiber T,Jung O,Dettmer K,Meister G,Bott M,Gronwald W,Sterner R

更新日期：2019-10-15 00:00:00

abstract：:The selenoamino acids methylselenocysteine (MeSeCys) and selenomethionine (SeMet) have disparate efficacies as anticancer agents. Herein, we use X-ray absorption spectroscopy to determine the chemical form of selenium in human neuroblastoma cells. Cells treated with MeSeCys contain a significant diselenide component, ...

journal_title：Biochemistry

authors： Weekley CM,Aitken JB,Musgrave IF,Harris HH

更新日期：2012-01-24 00:00:00

abstract：:We report on the determination of the solution structure of two sequence-related oligonucleotides, d(GTACGTAC)2 and d(CATCGATG)2. Results have been obtained by using a combined approach of (a) two-dimensional NMR, including proton and phosphorus experiments, (b) restrained molecular mechanics performed with sugar phas...

journal_title：Biochemistry

authors： Lefebvre A,Mauffret O,Hartmann B,Lescot E,Fermandjian S

更新日期：1995-09-19 00:00:00

abstract：:It is well-known, but little understood, that the nucleotide sequences between phased A(4-6)-tracts (at 10-11 bp intervals) have only a slight effect on overall curvature. To explore this phenomenon, we have examined the gel-migration properties of sequences containing both A-tracts as well as G-tracts (i.e., sequence...

journal_title：Biochemistry

authors： Merling A,Sagaydakova N,Haran TE

更新日期：2003-05-06 00:00:00

abstract：:Trypanosoma and Leishmania, pathogens responsible for diseases such as African sleeping sickness, Chagas' heart disease, or Oriental sore, are two of the very few genera that do not use the ubiquitous glutathione/glutathione reductase system to keep a stable cellular redox balance. Instead, they rely on trypanothione ...

journal_title：Biochemistry

authors： Stoll VS,Simpson SJ,Krauth-Siegel RL,Walsh CT,Pai EF

更新日期：1997-05-27 00:00:00

abstract：:The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP. Anionic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA protein to replicatively active ATP-DnaA protein in vitro, and thus likely play a k...

journal_title：Biochemistry

authors： Kitchen JL,Li Z,Crooke E

更新日期：1999-05-11 00:00:00

abstract：:Glycosylation is a common modification that can endow proteins with altered physical and biological properties. Ribonuclease 1 (RNase 1), which is the human homologue of the archetypal enzyme RNase A, undergoes N-linked glycosylation at asparagine residues 34, 76, and 88. We have produced the three individual glycofor...

journal_title：Biochemistry

authors： Kilgore HR,Latham AP,Ressler VT,Zhang B,Raines RT

更新日期：2020-09-01 00:00:00

abstract：:Structural and biochemical characterization of aspartate 152 at the distal heme side of catalase-peroxidase (KatG) from Synechocystis PCC 6803 reveals an important functional role for this residue. In the wild-type protein, the side chain carboxyl group of Asp152 is 7.8 A apart from the heme iron and is hydrogen-bonde...

journal_title：Biochemistry

authors： Jakopitsch C,Auer M,Regelsberger G,Jantschko W,Furtmüller PG,Rüker F,Obinger C

更新日期：2003-05-13 00:00:00

abstract：:Drug efflux and enzymatic drug degradation are two cellular mechanisms that contribute to drug resistance in many cancers. Herein, we report the synthesis and in vitro activity of a pro-immunostimulant that exploits both processes in tandem to selectively confer cancer-mediated immunogenicity. We demonstrate that an i...

journal_title：Biochemistry

authors： Burt AJ,Hantho JD,Nielsen AE,Mancini RJ

更新日期：2018-04-17 00:00:00

abstract：:Measurement of x-ray scattering at very small angles by solutions of myosin subfragment 1 (S1) yields a radius of gyration of 3.24 nm (mean) +/- 0.03 nm (standard error for N = 9). If S1 is assumed to be ellipsoid of revolution, of uniform electron density, with a molecular weight of 1.15 X 10(5) and a partial specifi...

journal_title：Biochemistry

authors： Kretzschmar KM,Mendelson RA,Morales MF

更新日期：1978-06-13 00:00:00

abstract：:Kinetics of reduction of phototrophic bacterial flavocytochromes c by exogenous flavin semiquinones and fully reduced flavins generated by laser flash photolysis have been studied. The mechanisms of reduction of Chromatium and Chlorobium flavocytochromes c are more similar to one another than previously thought. Neith...

journal_title：Biochemistry

authors： Cusanovich MA,Meyer TE,Tollin G

更新日期：1985-03-12 00:00:00

abstract：:The regiospecificity and stereospecificity of proton transfer in the yeast inorganic pyrophosphatase (PPase) catalyzed hydrolysis of P1,P2-bidentate Mg(H2O)4(PPi)2- were probed with exchange-inert metal complexes of imidodiphosphate (PNP) and thiopyrophosphate (PPS). PPase was unable to catalyze the hydrolysis of Mg(H...

journal_title：Biochemistry

authors： Lin I,Knight WB,Hsueh A,Dunaway-Mariano D

更新日期：1986-08-12 00:00:00