In vitro protein kinase C phosphorylation sites of placental lipocortin.

abstract：:Pterin-free inducible nitric oxide synthase (iNOS) was reconstituted with tetrahydrobiopterin (H(4)B) or tetrahydrobiopterin analogues (5-methyl-H(4)B and 4-amino-H(4)B), and the ability of bound 5-methyl-H(4)B and 4-amino-H(4)B to support catalysis by either full-length iNOS (FLiNOS) or the isolated heme domain (HDiN...

journal_title：Biochemistry

authors： Hurshman AR,Krebs C,Edmondson DE,Marletta MA

更新日期：2003-11-18 00:00:00

abstract：:Lysine side chains induce conformational changes in plasminogen (Pg) that regulate the process of fibrinolysis or blood clot dissolution. A lysine side-chain mimic, epsilon amino caproic acid (EACA), enhances the activation of Pg by urinary-type and tissue-type Pg activators but inhibits Pg activation induced by strep...

journal_title：Biochemistry

authors： Lin LF,Houng A,Reed GL

更新日期：2000-04-25 00:00:00

abstract：:Avian magnetoreception is assumed to occur in the retina. Although its molecular mechanism is unclear, magnetic field-dependent formation and the stability of radical-containing photointermediate(s) are suggested to play key roles in a hypothesis called the radical pair mechanism. Chicken cryptochrome4 (cCRY4) has bee...

journal_title：Biochemistry

authors： Otsuka H,Mitsui H,Miura K,Okano K,Imamoto Y,Okano T

更新日期：2020-09-29 00:00:00

abstract：:The bacterial alpha-lytic protease (alphaLP) is synthesized as a precursor containing a large N-terminal pro region (Pro) transiently required for correct folding of the protease [Silen, J. L., and Agard, D. A. (1989) Nature 341, 462-464]. Upon folding, the precursor is autocatalyticly cleaved to yield a tight-binding...

journal_title：Biochemistry

authors： Anderson DE,Peters RJ,Wilk B,Agard DA

更新日期：1999-04-13 00:00:00

abstract：:The effects of metal ion binding on the optical spectroscopic properties and temperature stability of two single tryptophan mutants of chicken skeletal TnC, F78W and F154W, have been examined. The absence of tyrosine and other tryptophan residues allowed the unambiguous assignment of the spectral signal from the intro...

journal_title：Biochemistry

authors： Moncrieffe MC,Venyaminov SY,Miller TE,Guzman G,Potter JD,Prendergast FG

更新日期：1999-09-14 00:00:00

abstract：:By use of site-directed mutagenesis, each prolyl residue in the lac permease of Escherichia coli at positions 28 (putative helix I), 31 (helix I), 61 (helix II), 89 (helix III), 97 (helix III), 123 (helix IV), 192 (putative hydrophilic region 7), 220 (helix VII), 280 (helix VIII), and 327 [helix X; Lolkema, J. S., et ...

journal_title：Biochemistry

authors： Consler TG,Tsolas O,Kaback HR

更新日期：1991-02-05 00:00:00

abstract：:The biochemical and structural properties of bovine retinal nucleoside diphosphate kinase were investigated. The enzyme showed two polypeptides of approximately 17.5 and 18.5 kDa on SDS-PAGE, while isoelectric focusing revealed seven to eight proteins with a pI range of 7.4-8.2. Sedimentation equilibrium yielded a mol...

journal_title：Biochemistry

authors： Abdulaev NG,Karaschuk GN,Ladner JE,Kakuev DL,Yakhyaev AV,Tordova M,Gaidarov IO,Popov VI,Fujiwara JH,Chinchilla D,Eisenstein E,Gilliland GL,Ridge KD

更新日期：1998-10-06 00:00:00

abstract：:The structural features of a chemically modified DNA template strand may promote error-prone DNA synthesis during replication. The resulting higher incidence of mutations, in turn, can eventually lead to tumor initiation. Structural insights into this process can be monitored by studying chemically modified base adduc...

journal_title：Biochemistry

authors： Cosman M,Hingerty BE,Geacintov NE,Broyde S,Patel DJ

更新日期：1995-11-21 00:00:00

abstract：:Human liver alanine aminopeptidase is inhibited by L-amino acids having hydrophobic side chains such as Phe, Tyr, Trp, Met, and Leu. Blocking of the amino group or the carboxyl group greatly reduces the inhibitory capacity of the amino acid. Kinetic studies demonstrate that inhibition of hydrolysis of the substrate L-...

journal_title：Biochemistry

authors： Garner CW,Behal FJ

更新日期：1975-07-15 00:00:00

abstract：:The recently identified Krokinobacter rhodopsin 2 (KR2) functions as a light-driven sodium ion pump. The structure of the retinal-binding pocket of KR2 offers important insights into the mechanisms of KR2, which has motif of Asn112, Asp116, and Gln123 (NDQ) that is common among sodium ion pump rhodopsins but is unique...

journal_title：Biochemistry

authors： Shigeta A,Ito S,Inoue K,Okitsu T,Wada A,Kandori H,Kawamura I

更新日期：2017-01-31 00:00:00

abstract：:The flavin-dependent quiescin-sulfhydryl oxidase (QSOX) inserts disulfide bridges into unfolded reduced proteins with the reduction of molecular oxygen to form hydrogen peroxide. This work investigates how QSOX and protein disulfide isomerase (PDI) cooperate in vitro to generate native pairings in two unfolded reduced...

journal_title：Biochemistry

authors： Rancy PC,Thorpe C

更新日期：2008-11-18 00:00:00

abstract：:The N-terminal 17% of apolipoprotein B (apoB-17) is secreted lipid-poor while apoB-41 particles are secreted with a triacylglycerol (TAG)-rich core. Thus, the sequence between apoB-17 and apoB-41 is necessary for the assembly of TAG-rich lipoproteins. To delineate this region, C127 cells were permanently transfected t...

journal_title：Biochemistry

authors： Carraway M,Herscovitz H,Zannis V,Small DM

更新日期：2000-08-15 00:00:00

abstract：:Maduropeptin (MDP) is a recently isolated antitumor antibiotic, consisting of an enediyne-containing chromophore embedded in a highly acidic protein. This holoantibiotic damages duplex DNA in vitro, producing a mixture of single- and double-strand breaks at selected sites. The chromophore, isolated as the methanol add...

journal_title：Biochemistry

authors： Zein N,Solomon W,Colson KL,Schroeder DR

更新日期：1995-09-12 00:00:00

abstract：:The molecular weight of phosphophoryn, an acidic phosphoprotein unique to dentin matrix, has been difficult to determine because of a combination of neutral protease activities in this tissue and the intrinsic high charge density of the molecule. In this study, bovine dentin phosphophoryn (BDPP) was isolated by a proc...

journal_title：Biochemistry

authors： Stetler-Stevenson WG,Veis A

更新日期：1983-08-30 00:00:00

abstract：:Legumain (asparaginyl endopeptidase) is the only protease with a preference for cleavage after asparagine residues. Increased legumain activity is a hallmark of inflammation, neurodegenerative diseases, and cancer, and legumain inhibitors have exhibited therapeutic effects in mouse models of these pathologies. Improve...

journal_title：Biochemistry

authors： Anderson BM,de Almeida LGN,Sekhon H,Young D,Dufour A,Edgington-Mitchell LE

更新日期：2020-01-28 00:00:00

abstract：:The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a ...

journal_title：Biochemistry

authors： Lu J,Zhao L,Xia A,Xia T,Qi X

更新日期：2013-03-12 00:00:00

abstract：:Silk fibroin exists in a number of different states, such as silk I and silk II, with different properties largely defined by differences in secondary structure composition. Numerous attempts have been made to control the transitions from silk I to silk II in vitro to produce high-performance materials. Of all the fac...

journal_title：Biochemistry

authors： Zhou P,Xie X,Knight DP,Zong XH,Deng F,Yao WH

更新日期：2004-09-07 00:00:00

abstract：:The mechanisms for substrate recognition by two cytoplasmic protein tyrosine phosphatases, PTP-5 and rrbPTP-1, were investigated. Phosphorylation sites on tyrosine-phosphorylated casein, a model PTP substrate, were characterized. Two peptides based on casein phosphorylation sites and one peptide based on the tyrosine ...

journal_title：Biochemistry

authors： Hippen KL,Jakes S,Richards J,Jena BP,Beck BL,Tabatabai LB,Ingebritsen TS

更新日期：1993-11-23 00:00:00

abstract：:DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli. We have analyzed the mechanism of binding of single-stranded DNA (ssDNA) by the DnaB x DnaC complex and DnaB helicase. Binding of ssDNA to DnaB he...

journal_title：Biochemistry

authors： Biswas SB,Biswas-Fiss EE

更新日期：2006-09-26 00:00:00

abstract：:Murine epidermal growth factor (EGF), a 53 amino acid protein, has been modified by enzymic digestion, site-specific chemical reactions, and recombinant DNA technology. After trypsin digestion the EGF derivatives EGF1-48 (called EGF-T) and EGF1-45 (called EGF-T2) were separated from the residual EGF and the C-terminal...

journal_title：Biochemistry

authors： Burgess AW,Lloyd CJ,Smith S,Stanley E,Walker F,Fabri L,Simpson RJ,Nice EC

更新日期：1988-07-12 00:00:00

abstract：:In order to define structural regions in thrombin that interact with hirudin, the N alpha-dinitrofluorobenzyl analogue of an undecapeptide was synthesized corresponding to residues 54-64 of hirudin [GDFEEIPEEY(O35SO3)L (DNFB-[35S]Hir54-64)]. DNFB-[35S]Hir54-64 was reacted at a 10-fold molar excess with human alpha-thr...

journal_title：Biochemistry

authors： Bourdon P,Fenton JW 2nd,Maraganore JM

更新日期：1990-07-10 00:00:00

abstract：:The value of reducing the activity of ornithine decarboxylase (ODC), a key enzyme in the biosynthesis of polyamines, is well-appreciated. Polyamines are necessary components for cell growth, and manipulation of polyamine homeostasis may be an effective strategy for the treatment of a number of disorders, including neo...

journal_title：Biochemistry

authors： Ackermann JM,Kanugula S,Pegg AE

更新日期：2005-02-15 00:00:00

abstract：:The association-dissociation kinetics of ribosomes from Escherichia coli have been studied under various conditions in a light-scattering stopped-flow apparatus. The dissociation reaction at 2 mg/ml at 25 degrees C, induced by lowering the MgCl2 concentration from 18 to 3 mM, can best be described by three independent...

journal_title：Biochemistry

authors： Görisch H,Goss DJ,Parkhurst LJ

更新日期：1976-12-28 00:00:00

abstract：:Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...

journal_title：Biochemistry

authors： Brautigan DL,Brown M,Grindrod S,Chinigo G,Kruszewski A,Lukasik SM,Bushweller JH,Horal M,Keller S,Tamura S,Heimark DB,Price J,Larner AN,Larner J

更新日期：2005-08-23 00:00:00

abstract：:Calcium protects a limited number of embryonic chick neural retina cell surface proteins from tryptic cleavage. One glycoprotein of Mr approximately 1.3 X 10(5) and pI approximately 4.8 (gp130) is present in intact retinas and, in the presence of Ca2+, is both resistant to tryptic cleavage and poorly iodinated. When c...

journal_title：Biochemistry

authors： Cook JH,Pratt RS,Lilien J

更新日期：1984-02-28 00:00:00

abstract：:Volumetric characteristics of protein recognition events determine the direction of pressure-induced shifts in the recognition reaction, while also providing insights into the structural, dynamic, and hydration changes. We report changes in volume, ΔV, and adiabatic compressibility, ΔK(S), accompanying the binding of ...

journal_title：Biochemistry

authors： Son I,Shek YL,Dubins DN,Chalikian TV

更新日期：2012-07-24 00:00:00

abstract：:The nerve growth factors (NGF's) isolated from cobra venom (Naja naja) and mouse submaxillary gland are closely related proteins. They are structurally similar in that about 60% of their amino acid residues are identical (Hogue-Angeletti, R. A., Frazier, W. A., Jacobs, J. W., Niall, H.D., and Bradshaw, R. A. (1976), B...

journal_title：Biochemistry

authors： Server AC,Herrup K,Shooter EM,Hogue-Angeletti RA,Frazier WA,Bradshaw RA

更新日期：1976-01-13 00:00:00

abstract：:The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha...

journal_title：Biochemistry

authors： Rusnak F,Faraci WS,Walsh CT

更新日期：1989-08-22 00:00:00

abstract：:The head of the P22 bacteriophage is interrupted by a unique dodecameric portal vertex that serves as a conduit for the entrance and exit of the DNA. Here, the in vitro unfolding/refolding processes of the portal protein of P22 were investigated at different temperatures (1, 25, and 37 degrees C) through the use of ur...

journal_title：Biochemistry

authors： Braga CA,Carvalho D,Lara FA,Cortines JR,Moore SD,Prevelige PE Jr,Foguel D

更新日期：2007-06-26 00:00:00

abstract：:We have investigated the effects of mixed Na+: Mg2+ ionic solutions on the stability of the nonspecific lac repressor-DNA complex. The effects of Mg2+ are simply interpreted in terms of its role as a competitor (with repressor) for DNA sites. From these studies, the binding constant of the Mg-DNA complex can be determ...

journal_title：Biochemistry

authors： Record MT Jr,deHaseth PL,Lohman TM

更新日期：1977-11-01 00:00:00