Abstract:
:The gene coding for the enzyme 2,3-dihydroxybenzoate-AMP ligase (2,3DHB-AMP ligase), responsible for activating 2,3-dihydroxybenzoic acid in the biosynthesis of the siderophore enterobactin, has been subcloned into the multicopy plasmid pKK223-3 and overproduced in a strain of Escherichia coli. The protein is an alpha 2 dimer with subunit molecular mass of 59 kDa. The enzyme catalyzes the exchange of [32P]pyrophosphate with ATP, dependent upon aromatic substrate with a turnover number of 340 min-1. The enzyme also releases pyrophosphate upon incubation with 2,3-dihydroxybenzoic acid and ATP; an initial burst corresponding to 0.7 nmol of pyrophosphate released per nanomole of enzyme is followed by a slower, continuous release with a turnover number of 0.41 min-1. The 1000-fold difference in rates observed between ATP-pyrophosphate exchange and continuous pyrophosphate release, as well as the close to stoichiometric amount of pyrophosphate released, suggests that intermediates are accumulating on the enzyme surface. Such intermediates have been observed and correspond to enzyme-bond (2,3-dihydroxybenzoyl)adenylate product.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Rusnak F,Faraci WS,Walsh CTdoi
10.1021/bi00443a008subject
Has Abstractpub_date
1989-08-22 00:00:00pages
6827-35issue
17eissn
0006-2960issn
1520-4995journal_volume
28pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Mammalian Na+/H+ exchangers (NHEs) play a fundamental role in cellular ion homeostasis. NHEs exhibit an appreciable variation in expression, regulation, and physiological function, dictated by their dynamics in subcellular localization and/or interaction with regulatory proteins. In recent years, a subgroup of NHEs co...
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi101082e
更新日期:2011-02-01 00:00:00
abstract::A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00628a023
更新日期:1977-05-03 00:00:00
abstract::The DNA binding domain (DBD) of p53 folds by a complex mechanism that involves parallel pathways and multiple intermediates, both on- and off-pathway. This heterogeneity renders DBD particularly susceptible to misfolding and aggregation. The origins of parallel folding mechanisms are not well understood. DBD folding h...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062106y
更新日期:2007-03-13 00:00:00
abstract::The membrane-active, cationic, β-hairpin peptide, arenicin, isolated from marine polychaeta Arenicola marina exhibits a broad spectrum of antimicrobial activity. The peptide in aqueous solution adopts the significantly twisted β-hairpin conformation without pronounced amphipathicity. To assess the mechanism of arenici...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200746t
更新日期:2011-07-19 00:00:00
abstract::We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00293a027
更新日期:1983-11-22 00:00:00
abstract::An endonuclease II preparation from Escherichia coli makes single strand breaks in DNA which has been treated with the carcinogen 7-bromomethyl-12-methylbenz[alpha]anthracene. In addition, the enzyme preparation excises N6-(12-methylbenz[alpha]anthracenyl-7-methyl)adenine and N2-(12-methylbenz[alpha]anthracenyl-7-met...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00697a002
更新日期:1975-12-30 00:00:00
abstract::The unique structural properties of the ferritin protein cages have provided impetus to focus on the methodical study of these self-assembling nanosystems. Among these proteins, Escherichia coli bacterioferritin (EcBfr), although architecturally very similar to other members of the family, shows structural instability...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200207w
更新日期:2011-05-17 00:00:00
abstract::Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051161o
更新日期:2005-11-01 00:00:00
abstract::Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00404a038
更新日期:1988-02-23 00:00:00
abstract::A novel liposomal method permits studies of Ca movements across the bilayers of multilamellar vesicles (MLV) which had entrapped the Ca-dependent, fluorescent indicator dye Fura 2. Ionomycin-mediated Ca translocation across MLV of phosphatidylcholine (PC)/dicetyl phosphate (DCP), 9:1, obeyed simple first-order kinetic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00415a040
更新日期:1988-07-26 00:00:00
abstract::Interconversion of protein conformations is imperative to function, as evidenced by conformational changes associated with enzyme catalytic cycles, ligand binding and post-translational modifications. In this study, we used 15N NMR relaxation experiments to probe the fast (i.e., ps-ns) and slow (i.e., micros-ms) confo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701425n
更新日期:2007-12-25 00:00:00
abstract::Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR. However, Taq polymerase belongs to the same DNA polymerase superfamily as the Molony murine leukemia virus reverse transcriptase and has in the past been sh...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00778
更新日期:2020-12-15 00:00:00
abstract::The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400053s
更新日期:2013-03-12 00:00:00
abstract::The rigor complexes that formed between rabbit skeletal muscle F-actin and chicken gizzard heavy meromyosin (HMM), in which the heavy chains had been cleaved with trypsin into 24K, 50K, and 68K fragments, were examined by using the zero-length chemical cross-linker 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC)...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00430a070
更新日期:1989-02-21 00:00:00
abstract::The gamma-aminobutyric acid transporter of rat brain synaptic vesicles was reconstituted in proteoliposomes, and its activity was studied in response to artificially created membrane potentials or proton gradients. Changes of the membrane potential were monitored using the dyes oxonol VI and 3,3'-diisopropylthiodicarb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00115a009
更新日期:1991-12-24 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) is a pro-inflammatory and tumor-promoting cytokine that occurs in two redox-dependent immunologically distinct conformational isoforms. The disease-related structural isoform of MIF (oxMIF) can be specifically and predominantly detected in the circulation of patients with i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b01156
更新日期:2018-03-06 00:00:00
abstract::The interaction of the recombinant hemoglobin from Vitreoscilla sp. (VHb) with the bacterial membrane of Escherichia coli cells has been investigated by measuring the propensity of VHb to interact with monolayers formed by natural bacterial phosholipids. The measurements showed that the protein is capable of penetrati...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052277n
更新日期:2006-04-04 00:00:00
abstract::An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00152a009
更新日期:1992-09-22 00:00:00
abstract::A GTP:RNA guanylyltransferase or capping enzyme has been purified approximately 2000-fold from wheat germ. The enzyme catalyzes the transfer of the GMP residue from GTP to the 5' end of RNA or synthetic polyribonucleotides. Diphosphate-ended polymers were capped more efficiently than molecules with triphosphate ends, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00531a020
更新日期:1982-01-19 00:00:00
abstract::MreB is a bacterial orthologue of actin that affects cell shape, polarity, and chromosome segregation. Although a significant body of work has explored its cellular functions, we know very little about the biochemical behavior of MreB. We have cloned, overexpressed in Escherichia coli, and purified untagged MreB1 from...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701538e
更新日期:2008-01-15 00:00:00
abstract::Quorum sensing, the ability of bacteria to sense their own population density through the synthesis and detection of small molecule signals, has received a great deal of attention in recent years. Acyl homoserine lactones (AHLs) are a major class of quorum sensing signaling molecules. In nature, some bacteria that do ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701945j
更新日期:2007-12-18 00:00:00
abstract::Two hybrid ribulose-1,5 bisphosphate carboxylase/oxygenase (RubisCO) enzymes were constructed using RubisCO small subunit genes (rbcS) from two eucaryotic marine organisms, Cylindrotheca sp. N1 and Olisthodiscus luteus, cloned downstream of the RubisCO large subunit gene (rbcL) of the cyanobacterium Synechococcus PCC ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00139a018
更新日期:1992-06-23 00:00:00
abstract::HPr is a protein of the bacterial phosphoenolpyruvate:sugar phosphotransferase transport system (PTS). In Gram-positive bacteria, HPr can be phosphorylated on Ser(46) by HPr(Ser) kinase/phosphorylase (HPrK/P) and on His(15) by enzyme I (EI) of the PTS. In vitro studies have shown that phosphorylation on one residue gr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060278p
更新日期:2006-05-30 00:00:00
abstract::Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoimidazole-4-carboxamide ribonucleotide (FAICAR) to IMP in the final step of de novo purine biosynthesis. Two major types of this enzyme have been discovered to date: PurH in Bacteria and Eukarya and PurO in Archaea. The structure of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061637j
更新日期:2007-05-01 00:00:00
abstract::DNA polymerase delta from calf thymus has been purified to apparent homogeneity by a new procedure which utilizes hydrophobic interaction chromatography with phenyl-Sepharose at an early step to separate most of the calcium-dependent protease activity from DNA polymerase delta and alpha. The purified enzyme migrates a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00304a003
更新日期:1984-04-24 00:00:00
abstract::RSC (remodel the structure of chromatin) is an essential chromatin remodeler of Saccharomyces cerevisiae that has been shown to have DNA translocase properties. We studied the DNA binding properties of a "trimeric minimal RSC" (RSCt) of the RSC chromatin remodeling complex and the effect of nucleotides on this interac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200837b
更新日期:2011-09-20 00:00:00
abstract::The immunophilin FKBP52 interacts with nuclear steroid hormone receptors. Studying the crystal structure of human estrogen receptor α (hERα) and using nuclear magnetic resonance, we show here that the short V(364)PGF(367) sequence, which is located within its ligand-binding domain and adopts a type II β-turn conformat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00506
更新日期:2016-09-27 00:00:00
abstract::Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b00988
更新日期:2015-12-22 00:00:00
abstract::The interaction of bovine spleen profilin with ATP- and ADP-G-actin and poly(L-proline) has been studied by spectrofluorimetry, analytical ultracentrifugation, and rapid kinetics in low ionic strength buffer. Profilin binding to G-actin is accompanied by a large quenching of tryptophan fluorescence, allowing the measu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00194a011
更新日期:1994-07-19 00:00:00
abstract::N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. The...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00222a012
更新日期:1991-02-26 00:00:00