Purification and characterization of the messenger ribonucleic acid capping enzyme GTP:RNA guanylyltransferase from wheat germ.

abstract：:Ethanol, in vitro, is known to perturb the molecular order of the phospholipids in biological membranes, while chronic ethanol exposure, in vivo, leads to resistance to disordering. Such changes have usually been measured by electron spin resonance, utilizing fatty acid spin probes. The use of such probes is controver...

journal_title：Biochemistry

authors： Taraschi TF,Wu A,Rubin E

更新日期：1985-12-03 00:00:00

abstract：:NahE and PhdJ are bifunctional hydratase-aldolases in bacterial catabolic pathways for naphthalene and phenanthrene, respectively. Bacterial species with these pathways can use polycyclic aromatic hydrocarbons (PAHs) as sole sources of carbon and energy. Because of the harmful properties of PAHs and their widespread d...

journal_title：Biochemistry

authors： LeVieux JA,Medellin B,Johnson WH Jr,Erwin K,Li W,Johnson IA,Zhang YJ,Whitman CP

更新日期：2018-06-26 00:00:00

abstract：:The ability of avian-specific linker histone H5, and the globular domains of H5 (GH5) and H1(0) (GH1(0), to self-associate either free in solution or when bound to DNA was investigated. All three proteins underwent a salt-dependent increase in turbidity that may be indicative of nonspecific interactions. Dithiobis(suc...

journal_title：Biochemistry

authors： Carter GJ,van Holde K

更新日期：1998-09-08 00:00:00

abstract：:Previous studies have demonstrated that lung-specific proteins are associated with surfactant lipids, particularly the highly surface active subfraction known as tubular myelin. We have isolated a surfactant-associated protein complex with molecular weight components of 36 000, 32 000, and 28 000 and reassembled it wi...

journal_title：Biochemistry

authors： Hawgood S,Benson BJ,Hamilton RL Jr

更新日期：1985-01-01 00:00:00

abstract：:Aminopeptidases catalyze the hydrolysis of amino acid residues from the amino terminus of peptide substrates. They are found in most cells and tissues, and their activity has been implicated in myriad fundamental biochemical and physiological processes. Nevertheless, little is known about the structure of the aminopep...

journal_title：Biochemistry

authors： Taylor A,Peltier CZ,Jahngen EG Jr,Laxman E,Szewczuk Z,Torre FJ

更新日期：1992-04-28 00:00:00

abstract：:The effects of GDP and of aurodox (N-methylkirromycin) on the affinity of elongation factor Tu (EF-Tu) for aminoacyl-tRNA (aa-tRNA) have been quantified spectroscopically by using Phe-tRNA(Phe)-Fl8, a functionally active analogue of Phe-tRNA(Phe) with a fluorescein dye convalently attached to the s4U-8 base. The assoc...

journal_title：Biochemistry

authors： Dell VA,Miller DL,Johnson AE

更新日期：1990-02-20 00:00:00

abstract：:We have studied the transient kinetics of electron transfer from a positively charged viologen analogue (propylene diquat), reduced by pulsed laser excitation of the deazariboflavin/EDTA system, to the net negatively charged ferredoxins from spinach and Clostridium pasteurianum. Spinach ferredoxin showed monophasic ki...

journal_title：Biochemistry

authors： Navarro JA,Cheddar G,Tollin G

更新日期：1989-07-11 00:00:00

abstract：:The DNA binding of three platinum(II) intercalation reagents has been studied and found to depend upon base composition, the nature of the intercalator, and the ionic strength of the solvent medium. In 0.2 M NaCl, binding data for calf thymus DNA show the association constants to be approximately 10(4) M-1. The bindin...

journal_title：Biochemistry

authors： Howe-Grant M,Lippard SJ

更新日期：1979-12-25 00:00:00

abstract：:Tombusviruses use a 19 kDa protein (p19) as a suppressor of the RNA silencing pathway during infection. The p19 protein binds to short-interfering RNA (siRNA) as a dimer and shows a high selectivity for short duplex RNAs over other RNA species. Since p19 can bind to synthetic and RNA silencing generated small RNAs wit...

journal_title：Biochemistry

authors： Cheng J,Sagan SM,Jakubek ZJ,Pezacki JP

更新日期：2008-08-05 00:00:00

abstract：:Peptide-membrane interactions are important for understanding the binding, partitioning, and folding of membrane proteins; the activity of antimicrobial and fusion peptides; and a number of other processes. We describe molecular dynamics simulations (10-25 ns) of two pentapeptides Ace-WLXLL (with X = Arg or Lys side c...

journal_title：Biochemistry

authors： Aliste MP,MacCallum JL,Tieleman DP

更新日期：2003-08-05 00:00:00

abstract：:A gentle and rapid isolation procedure is described yielding fractions containing better than 95% pure OmpF porin of Escherichia coli BE with different amounts of bound lipopolysaccharide (LPS). The procedure employs continuous free-flow electrophoresis (FFE) in the presence of detergent above its critical micelle con...

journal_title：Biochemistry

authors： Holzenburg A,Engel A,Kessler R,Manz HJ,Lustig A,Aebi U

更新日期：1989-05-16 00:00:00

abstract：:The importance of a specific hydrogen bond between thermolysin and a phosphonamidate inhibitor, Z-NHCH2-PO(O-)-Leu-Leu (1) [Bartlett, P. A., & Marlowe, C. K. (1987) Science (Washington D.C.) 235, 569-571], has been reevaluated. We have determined the inhibition constants (binding free energies) for thermolysin of phos...

journal_title：Biochemistry

authors： Grobelny D,Goli UB,Galardy RE

更新日期：1989-06-13 00:00:00

abstract：:Anaerobic induction of light harvesting and reaction center gene expression involves two transacting factors termed RegA and RegB. Sequence and mutational analysis has indicated that RegA and RegB constitute cognate components of a prokaryotic sensory transduction cascade with RegB comprising a membrane-spanning senso...

journal_title：Biochemistry

authors： Inoue K,Kouadio JL,Mosley CS,Bauer CE

更新日期：1995-01-17 00:00:00

abstract：:This paper presents the crystal structure of porcine pancreatic carboxypeptidase B (pp-CpB) in complex with a variety of thiol-based inhibitors that were developed as antagonists of activated thrombin-activatable fibrinolysis inhibitor (TAFIa). Recent studies have indicated that a selective inhibitor of TAFIa could en...

journal_title：Biochemistry

authors： Adler M,Bryant J,Buckman B,Islam I,Larsen B,Finster S,Kent L,May K,Mohan R,Yuan S,Whitlow M

更新日期：2005-07-05 00:00:00

abstract：:The subtilisins have an extended substrate binding cleft comprising at least 8 subsites. Two pockets at the S1 and S4 sites are particularly conspicuous, and the interactions between substrate and these two pockets are very important for the substrate specificity. Phe residues have mutationally been introduced at one ...

journal_title：Biochemistry

authors： Bech LM,Sørensen SB,Breddam K

更新日期：1993-03-23 00:00:00

abstract：:Type VI collagen, prepared from guanidine extracts of human amnion, contains very little monomeric material, the major forms being dimers and tetramers. In order to study the orientation of the molecules in these aggregates, they were digested with pepsin followed by bacterial collagenase. Two fragments were isolated,...

journal_title：Biochemistry

authors： Kuo HJ,Keene DR,Glanville RW

更新日期：1989-05-02 00:00:00

abstract：:In bee venom phospholipase A2, histidine-34 probably functions as a Brønsted base to deprotonate the attacking water. Aspartate-64 and tyrosine-87 form a hydrogen bonding network with histidine-34. We have prepared mutants at these positions and studied their kinetic properties. The mutant in which histidine-34 is cha...

journal_title：Biochemistry

authors： Annand RR,Kontoyianni M,Penzotti JE,Dudler T,Lybrand TP,Gelb MH

更新日期：1996-04-09 00:00:00

abstract：:Multiply branched polylactosaminoglycans are expressed in glycoproteins and glycolipids of many cells. Interest in their biology stems from their abundant expression in early embryonal cells and from their ability to carry multiple lectin-binding determinants, which makes them prominent ligands and antagonists of cell...

journal_title：Biochemistry

authors： Leppanen A,Salminen H,Zhu Y,Maaheimo H,Helin J,Costello CE,Renkonen O

更新日期：1997-06-10 00:00:00

abstract：:The instantaneous electrochromic response of carotenoids associated with the B800-850 light-harvesting complex of Rhodobacter sphaeroides has been used widely as an intrinsic probe of membrane potential. In the present study, the structural basis for this phenomenon was examined by phospholipase A2 digestion of chroma...

journal_title：Biochemistry

authors： Olivera LM,Niederman RA

更新日期：1993-01-26 00:00:00

abstract：:A T203Y substitution in green fluorescent protein causes a red shift in emission to yield a class of mutants known as yellow fluorescent protein (YFP). Many of these YFP mutants bind halides with affinities in the millimolar range, which often results in the chromophore pK values being shifted into the physiological r...

journal_title：Biochemistry

authors： Seward HE,Basran J,Denton R,Pfuhl M,Muskett FW,Bagshaw CR

更新日期：2013-04-09 00:00:00

abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

journal_title：Biochemistry

authors： Brisson JR,Carver JP

更新日期：1983-07-19 00:00:00

abstract：:The NADH absorbance spectrum of nicotinoprotein (NADH-containing) alcohol dehydrogenase from Amycolatopsis methanolica has a maximum at 326 nm. Reduced enzyme-bound pyridine dinucleotide could be reversibly oxidized by acetaldehyde. The fluorescence excitation spectrum for NADH bound to the enzyme has a maximum at 325...

journal_title：Biochemistry

authors： Piersma SR,Visser AJ,de Vries S,Duine JA

更新日期：1998-03-03 00:00:00

abstract：:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...

journal_title：Biochemistry

authors： Serrano P,Hammond JA,Geralt M,Wüthrich K

更新日期：2018-03-13 00:00:00

abstract：:The binding of ethidium bromide, as monitored by fluorescence enhancement, to chromatin prepared by nuclease digestion has been compared with the binding of the dye to sheared chromatin. The nuclease preparation (native chromatin) is characterized by a high affinity region of the Scatchard plot (r = 0-0.025, K1 = 1 X ...

journal_title：Biochemistry

authors： Paoletti J,Magee BB,Magee PT

更新日期：1977-02-08 00:00:00

abstract：:The N-terminal region of alpha-spectrin is responsible for its association with beta-spectrin in a heterodimer, forming functional tetramers. Non-erythroid alpha-spectrin (alphaII-spectrin) has a significantly higher association affinity for beta-spectrin than the homologous erythroid alpha-spectrin (alphaI-spectrin)....

journal_title：Biochemistry

authors： Li Q,Fung LW

更新日期：2009-01-13 00:00:00

abstract：:By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...

journal_title：Biochemistry

authors： Singh AK,Ekka MK,Kaushik A,Pandya V,Singh RP,Banerjee S,Mittal M,Singh V,Kumaran S

更新日期：2017-09-19 00:00:00

abstract：:To understand the relationship between the chemical structure of polar molecules and their membrane location, the behavior of dansyl (dimethylaminonaphthalenesulfonyl) and related polar fluorescent probes was examined. The depth of these probes in lipid bilayers was determined by parallax analysis of fluorescence quen...

journal_title：Biochemistry

authors： Asuncion-Punzalan E,Kachel K,London E

更新日期：1998-03-31 00:00:00

abstract：:Human fibroblast interferon binds to a concanavalin A-agarose (Con A-Sepharose) equilibrated with methyl alpha-D-mannopyranoside, or levan; in contrast, it is only partially retarded on a similar column equilibrated with ethylene glycol. Interferon does not bind, however, to a lectin column equilibrated with both meth...

journal_title：Biochemistry

authors： Davey MW,Sulkowski E,Carter WA

更新日期：1976-02-10 00:00:00

abstract：:The 14-3-3 proteins interact with diverse cellular molecules involved in various signal transduction pathways controlling cell proliferation, transformation, and apoptosis. To aid our investigation of the biological function of 14-3-3 proteins, we have set out to identify high-affinity antagonists. By screening phage ...

journal_title：Biochemistry

authors： Wang B,Yang H,Liu YC,Jelinek T,Zhang L,Ruoslahti E,Fu H

更新日期：1999-09-21 00:00:00

abstract：:We have studied the physical and functional linkages of heterologously expressed human 5-HT1A receptors to G protein alpha-subunits in HeLa and CHO-K1 cells. HeLa cells expressed immunoreactivity to G(i) proteins with an apparent rank order of G(i) alpha 3 (approximately 1 pmol/mg of protein) > G(i) alpha 1 (approxima...

journal_title：Biochemistry

authors： Raymond JR,Olsen CL,Gettys TW

更新日期：1993-10-19 00:00:00