Abstract：
:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing factor were found to individually bind RNAs with micromolar affinities. It was thus of interest to further investigate the structural basis of the well-documented high-affinity RNA recognition by RMB10. Here, we investigated RNA binding by combinations of two or three of these domains and discovered that a polypeptide containing RRM1, ZnF1, and RRM2 connected by their natural linkers recognizes a specific sequence of the Fas exon 6 mRNA with an affinity of 20 nM. Nuclear magnetic resonance structures of the RBM10 domains RRM1 and ZnF1 and the natural V354del isoform of RRM2 further confirmed that the interactions with RNA are driven by canonical RNA recognition elements. The well-known high-fidelity RNA splice site recognition by RBM10, and probably by RBM5 and RBM6, can thus be largely rationalized by a cooperative binding action of RRM and ZnF domains.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Serrano P,Hammond JA,Geralt M,Wüthrich Kdoi
10.1021/acs.biochem.7b01242subject
Has Abstractpub_date
2018-03-13 00:00:00pages
1563-1567issue
10eissn
0006-2960issn
1520-4995journal_volume
57pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The protein product of the Methanococcus jannaschii MJ0768 gene has been expressed in Escherichia coli, purified to homogeneity, and shown to catalyze the GTP-dependent addition of two l-glutamates to the l-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F(420)-0) to form F(420)-0-glutamyl-glutama...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034779b
更新日期：2003-08-19 00:00:00
abstract：:A 19-amino acid residue peptide, Gly-Trp-Leu-Lys-Ile-Lys-Ala-Ala-Met-Arg-Trp-Gly-Phe-Phe-Val-Arg-Lys-Lys- Ala, corresponding to the basic amphiphilic alpha-helix (BAA) motif at the C-terminus of a recombinant tobacco calmodulin-binding protein, TCB60, was synthesized. The interaction of the synthetic binding domain wi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9621674
更新日期：1997-02-25 00:00:00
abstract：:Vangl1 and Vangl2 are membrane proteins that play an important role in neurogenesis, and Vangl1/Vangl2 mutations cause neural tube defects in mice and humans. At the cellular level, Vangl proteins regulate the establishment of planar cell polarity (PCP), a process requiring membrane assembly of asymmetrically distribu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101767a
更新日期：2011-03-29 00:00:00
abstract：:To develop molecules capable of directly probing the catechol binding region of the beta(2)-adrenergic receptor (beta(2)AR), novel benzophenone- and fluorenone-based beta(2)AR antagonists were prepared as potential photoaffinity probes. While the benzophenone-containing ligands bound with relatively modest affinity, o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi001342k
更新日期：2000-10-24 00:00:00
abstract：:The precipitation mechanism of alpha-globulin in the presence of myo-inositol hexaphosphate (sodium phytate) was studied in detail. The maximum interaction was found at pH 2.3 where the protein was in a dissociated state having an 8.3S aggregate and a 1.5S monomer. This interaction was predominantly dependent upon the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00064a035
更新日期：1993-04-06 00:00:00
abstract：:Acyl carrier protein (ACP) is the central player in fatty acid (FA) biosynthesis. It covalently binds all FA intermediates and presents them to the enzymes needed for elongation. Bacterial ACP must interact with a large number of proteins, which raises the question of how different acyl-ACPs are recognized and disting...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901713r
更新日期：2010-04-06 00:00:00
abstract：:The hammerhead ribozyme undergoes a well-defined two-stage conformational folding process, induced by the binding of magnesium ions. In this study, we have used isothermal titration calorimetry to analyze the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme. Binding to the natural ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi002231o
更新日期：2001-02-06 00:00:00
abstract：:Z-disc-associated, alternatively spliced, PDZ motif-containing protein (ZASP) is a principal component of the sarcomere. The three prevalent isoforms of ZASP in skeletal muscle are generated by alternative splicing of exons 9 and 10. The long isoforms, either having (ZASP-L) or lacking exon 10 (ZASP-LΔex10), include a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00067
更新日期：2017-04-11 00:00:00
abstract：:Although the short-chain dehydrogenase/reductase (SDR) superfamily contains a very large number of members defined in annotated databases and by biochemical and structural studies, very few SDR enzymes have been identified that have a homologous partner catalyzing the same reaction but with an opposite stereospecifici...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0118005
更新日期：2002-02-26 00:00:00
abstract：:Raman difference spectroscopy is used to probe the properties of a 36-nt RNA molecule, "D5", which lies at the heart of the catalytic apparatus in group II introns. For D5 that has all of its adenine residues labeled with (13)C and (15)N and utilizing Raman difference spectroscopy, we identify the conformationally sen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi902117w
更新日期：2010-04-27 00:00:00
abstract：:We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00297a005
更新日期：1984-01-17 00:00:00
abstract：:Membrane-bound guanylate cyclases harbor a region called the dimerization or linker domain, which aids the enzymes in adopting an optimal monomer-monomer arrangement for catalysis. One subgroup of these guanylate cyclases is expressed in rod and cone cells of vertebrate retina, and mutations in the dimerization domain...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi400288p
更新日期：2013-07-30 00:00:00
abstract：:Plasmids containing a site-specifically incorporated O6-methyl- (m6G), O6-ethyl- (e6G), or O6-benzylguanine (b6G) within the ATG initiation codon of the lacZ' gene were used to transform Escherichia coli that were repair proficient or deficient in one or both of the E. coli O6-alkylguanine-DNA alkyltransferases, the u...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00197a020
更新日期：1994-08-09 00:00:00
abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00026a009
更新日期：1995-07-04 00:00:00
abstract：:The peptide portion of the lipopeptide isolated from bovine myelin basic protein contained glycine, lysine, and serine in a 2:1:1 molar ratio as determined by amino acid analysis. The N-terminus of the peptide was determined to be glycine. The tetrapeptide Gly53-Ser-Gly-Lys56 was the only segment of myelin basic prote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00357a059
更新日期：1986-05-06 00:00:00
abstract：:Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00458a021
更新日期：1990-02-13 00:00:00
abstract：:Synthetic photocontrolled proteins could be powerful tools for probing cellular chemistry. Several previous attempts to produce such systems by incorporating photoisomerizable chromophores into biomolecules have led to photocontrol but with incomplete reversibility, where the chromophore becomes trapped in one photois...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi060142r
更新日期：2006-05-16 00:00:00
abstract：:X-ray studies of the proofreading 3',5'-exonuclease site of the large (Klenow) fragment of DNA polymerase I have detected a binuclear metal complex consisting of a pentacoordinate metal (site A) which shares a ligand, Asp-355, with an octahedral metal (site B) [Freemont, P. S., Friedman, J. M., Beese, L. S., Sanderson...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00110a012
更新日期：1991-11-19 00:00:00
abstract：:The vitamin K epoxide reductase (VKORC1) enzyme is of primary importance in many physiological processes, i.e., blood coagulation, energy metabolism, and arterial calcification prevention, due to its role in the vitamin K cycle. Indeed, VKORC1 catalyzes reduction of vitamin K epoxide to quinone and then to hydroquinon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b01084
更新日期：2020-04-07 00:00:00
abstract：:Early studies involving purine salvage in Salmonella typhimurium resulted in the isolation and identification of a mutant strain possessing a genetically modified hypoxanthine phosphoribosyl-transferase (HPRT) with enhanced substrate specificity for guanine [Benson, C. E., and Gots, J. S. (1975) J. Bacteriol. 121, 77-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9720179
更新日期：1998-03-10 00:00:00
abstract：:The human antioxidant protein, HAH1, is an important participant in a Cu(I) transport chain, delivering one Cu(I) ion to the Wilson's (WND) or Menkes disease protein (MNK). Full geometry optimizations and second-derivative calculations were performed on several binding site models using the B3LYP functional to derive ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7007195
更新日期：2007-07-31 00:00:00
abstract：:A novel and efficient enzyme kinetics assay using electrospray ionization mass spectrometry was developed and applied to the bacterial carbohydrate sulfotransferase (NodST). NodST catalyzes the sulfuryl group transfer from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to chitobiose, generating 3'-phosphoadenosine 5'-ph...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi020457g
更新日期：2002-11-05 00:00:00
abstract：:The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972163e
更新日期：1998-01-06 00:00:00
abstract：:An aminoglycoside 3-acetyltransferase [AAC(3)], possibly a new isoenzymic species of the 3-N-acetyltransferase group, was purified to apparent homogeneity from a crude extract of Pseudomonas aeruginosa, a gentamicin-resistant clinical isolate. The method of purification was consecutive column chromatography--(i) gel f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00534a009
更新日期：1982-03-02 00:00:00
abstract：:Cytochrome c has been chemically modified by methylene blue mediated photooxidation. It is established that the methionine residues of the protein have been specifically converted to methionine sulfoxide residues. No oxidation of any other amino acid residues or the cysteine thioether bridges of the molecule occurs du...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00650a029
更新日期：1976-03-09 00:00:00
abstract：:Thyroxine and analogues inhibit rat liver aminoacyl-tRNA synthetase activity for phenylalanine and tyrosine. A high yield purification of the major cytoplasmic form of phenylalanyl-tRNA synthetase (C1) and its characterization is reported. Polyribosome-bound and other sedimentable forms are found to be indistinguishab...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00647a017
更新日期：1976-01-27 00:00:00
abstract：:Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00691a008
更新日期：1975-10-07 00:00:00
abstract：:Succinyl-CoA:3-oxoacid coenzyme A transferase (EC 2.8.3.5), which catalyzes the reversible conversion of succinyl-CoA and acetoacetate into acetoacetyl-CoA and succinate through a covalent enzyme thiol ester intermediate, E-CoA, utilizes binding energy from noncovalent interactions with CoA to bring about an increase ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00037a005
更新日期：1995-09-19 00:00:00
abstract：:Interconversion of protein conformations is imperative to function, as evidenced by conformational changes associated with enzyme catalytic cycles, ligand binding and post-translational modifications. In this study, we used 15N NMR relaxation experiments to probe the fast (i.e., ps-ns) and slow (i.e., micros-ms) confo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701425n
更新日期：2007-12-25 00:00:00
abstract：:The ribosome-dependent stringent factor reaction was found to be nonspecific with regard to the number of phosphate groups linked to the 5' position of guanosine nucleotides. Both GMP and guanosine 5'-tetraphosphate could accept a pyrophosphoryl group from ATP although at a much lower rate than GDP or GTP. Guanosine 5...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00676a015
更新日期：1975-03-11 00:00:00
