Characterization of the basic amphiphilic alpha-helix calmodulin-binding domain of a 61.5 kDa tobacco calmodulin-binding protein.

abstract：:ZntA from Escherichia coli confers resistance to toxic concentrations of Pb2+, Zn2+, and Cd2+. It is a member of the P1B-ATPase transporter superfamily, which includes the human Cu+-transporting proteins ATP7A and ATP7B. P1B-type ATPases typically have a hydrophilic N-terminal metal-binding domain and eight transmembr...

journal_title：Biochemistry

authors： Roberts CS,Muralidharan S,Ni F,Mitra B

更新日期：2020-12-01 00:00:00

abstract：:Terminase is an enzyme from bacteriophage lambda that is required for insertion of the viral genome into an empty pro-capsid. This enzyme is composed of the viral proteins gpNu1 (20.4 kDa) and gpA (73.3 kDa) in a holoenzyme complex. Current models for terminase assembly onto DNA suggest that gpNu1 binds to three repea...

journal_title：Biochemistry

authors： Yang Q,de Beer T,Woods L,Meyer JD,Manning MC,Overduin M,Catalano CE

更新日期：1999-01-05 00:00:00

abstract：:Five previously unknown hemorrhagic proteins, designated hemorrhagic toxins a,b,c,d, and e, were isolated from the venom of the western diamondback rattlesnake (Crotalus atrox). Molecular weights of hemorrhagic toxins a-e were determined to be 68 000, 24 000, 24 000, 24 000, and 25 700, respectively, by sodium dodecyl...

journal_title：Biochemistry

authors： Bjarnason JB,Tu AT

更新日期：1978-08-08 00:00:00

abstract：:The differential scanning calorimetry trace of F1ATPase, prepared from beef heart submitochondrial particles, has a single sharp endothermic transition at 80.5 +/- 1.0 degrees C and a half-height peak width of 2.0 +/- 0.2 degrees. The transition enthalpy is 19 +/- 2 cal/g of protein. Submitochondrial particles (SMP) h...

journal_title：Biochemistry

authors： Kresheck GC,Adade AB,Vanderkooi G

更新日期：1985-03-26 00:00:00

abstract：:Vibrational Raman spectroscopic experiments have been performed as a function of temperature on aqueous dispersions of synthetic DL-erythro-N-lignoceroylsphingosylphosphocholine [C(24):SPM], a racemic mixture of two highly asymmetric hydrocarbon chain length sphingomyelins. Raman spectral peak-height intensity ratios ...

journal_title：Biochemistry

authors： Levin IW,Thompson TE,Barenholz Y,Huang C

更新日期：1985-10-22 00:00:00

abstract：:Thermal transitions of type I collagen fibrils were investigated by differential scanning calorimetry and spectrophotometry of turbidity within a wide range of external conditions. The advanced microcalorimeter allowed us to carry out the measurements at low concentrations of collagen (0.15-0.3 mg/mL). At these concen...

journal_title：Biochemistry

authors： Tiktopulo EI,Kajava AV

更新日期：1998-06-02 00:00:00

abstract：:RPE65, a protein expressed in cells of the retinal pigment epithelium of the eye, is essential for the synthesis by isomerohydrolase of 11-cis-retinal, the chromophore of rod and cone opsins. Recent work has established that RPE65 is a retinyl ester binding protein, and as all-trans-retinyl esters are the substrate fo...

journal_title：Biochemistry

authors： Lyubarsky AL,Savchenko AB,Morocco SB,Daniele LL,Redmond TM,Pugh EN Jr

更新日期：2005-07-26 00:00:00

abstract：:Vascular adhesion molecules regulate the migration of leukocytes from the blood into tissue during inflammation. Binding of leukocytes to vascular cell adhesion molecule-1 (VCAM-1) activates signals in endothelial cells, including Rac1 and calcium fluxes. These VCAM-1 signals are required for leukocyte transendothelia...

journal_title：Biochemistry

authors： Marchese ME,Berdnikovs S,Cook-Mills JM

更新日期：2012-10-16 00:00:00

abstract：:The solution structure of the complex formed between an oligodeoxynucleotide containing a two-base bulge (5'-CCATCGTCTACCTTTGGTAGGATGG) and SCA-alpha2, a designed spirocyclic helical molecule, has been elucidated. SCA-alpha2, a close mimic of the metabolite, NCSi-gb, of the DNA bulge-specific enediyne antibiotic neoca...

journal_title：Biochemistry

authors： Zhang N,Lin Y,Xiao Z,Jones GB,Goldberg IH

更新日期：2007-04-24 00:00:00

abstract：:To study fibronectin (FN) conformation and assembly, we generated several deletion mutants: FNΔI1-5, FNΔIII1-3, FNΔIII4-8, and FNΔIII11-14. A monomeric form, FNmono, which lacked the C-terminal dimerization region, was also created. FNtnA-D was generated by swapping FNIII domains 1-8 in FNΔIII11-14 with seven FNIII do...

journal_title：Biochemistry

authors： Ohashi T,Lemmon CA,Erickson HP

更新日期：2017-08-29 00:00:00

abstract：:Our previous studies demonstrated that the first 1000 amino acid residues (the βα1 domain) of human apolipoprotein (apo) B-100, termed apoB:1000, are required for the initiation of lipoprotein assembly and the formation of a monodisperse stable phospholipid (PL)-rich particle. The objectives of this study were (a) to ...

journal_title：Biochemistry

authors： Manchekar M,Kapil R,Sun Z,Segrest JP,Dashti N

更新日期：2017-08-08 00:00:00

abstract：:A striking feature of the CLIC (chloride intracellular channel) protein family is the ability of its members to convert between a soluble state and an integral membrane channel form. Direct evidence of the structural transition required for the CLIC protein to autonomously insert into the membrane is lacking, largely ...

journal_title：Biochemistry

authors： Goodchild SC,Howell MW,Littler DR,Mandyam RA,Sale KL,Mazzanti M,Breit SN,Curmi PM,Brown LJ

更新日期：2010-06-29 00:00:00

abstract：:The phosphoryl-binding elements in the GDP-binding domain of elongation factor Tu were studied by heteronuclear proton observe methods. Five proton resonances were found below 10.5 ppm. Two of these were assigned to the amide groups of Lys 24 and Gly 83. These are conserved residues in each of the consensus sequences....

journal_title：Biochemistry

authors： Lowry DF,Cool RH,Redfield AG,Parmeggiani A

更新日期：1991-11-12 00:00:00

abstract：:Escherichia coli DNA photolyase contains 1,5-dihydro-FAD (FADH2) plus 5,10-methenyl-tetrahydrofolate (5,10-CH+-H4folate). Both chromophores are fluorescent, and either can function as a sensitizer in catalysis. At 77 K separate fluorescence emission bands are observed for FADH2 (lambda max = 505 nm, shoulder at 540 nm...

journal_title：Biochemistry

authors： Jordan SP,Jorns MS

更新日期：1988-12-13 00:00:00

abstract：:Blastochloris tepida is a newly described thermophilic purple bacterium containing bacteriochlorophyll b. Using purified light-harvesting 1 reaction center (LH1-RC) core complexes from Blc. tepida, we compared the biochemical, spectroscopic, and thermal denaturation properties of these complexes with those of its meso...

journal_title：Biochemistry

authors： Seto R,Takaichi S,Kurihara T,Kishi R,Honda M,Takenaka S,Tsukatani Y,Madigan MT,Wang-Otomo ZY,Kimura Y

更新日期：2020-06-30 00:00:00

abstract：:The peptidolytic reaction of HIV-1 protease has been investigated by using four oligopeptide substrates, Ac-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Arg-Ala-Ser-Gln-Asn-Tyr-Pro-Val-Val-NH2, Ac-Ser-Gln-Ser-Tyr-Pro-Val-Val-NH2, and Ac-Arg-Lys-Ile-Leu-Phe-Leu-Asp-Gly-NH2, that resemble two cleavage sites found within the natu...

journal_title：Biochemistry

authors： Hyland LJ,Tomaszek TA Jr,Roberts GD,Carr SA,Magaard VW,Bryan HL,Fakhoury SA,Moore ML,Minnich MD,Culp JS

更新日期：1991-08-27 00:00:00

abstract：:Cytochrome P450 (P450) 3A4 is the most abundant human P450 and oxidizes a diversity of substrates, including various drugs, steroids, carcinogens, and macrolide natural products. In some reactions, positive cooperativity has been reported in microsomal studies. Flavonoids, e.g., 7,8-benzoflavone (alpha-naphthoflavone,...

journal_title：Biochemistry

authors： Ueng YF,Kuwabara T,Chun YJ,Guengerich FP

更新日期：1997-01-14 00:00:00

abstract：:The influenza M2 protein is the target of the amantadine family of antiviral drugs, and its transmembrane (TM) domain structure and dynamics have been extensively studied. However, little is known about the structure of the highly conserved N-terminal ectodomain, which contains epitopes targeted by influenza vaccines....

journal_title：Biochemistry

authors： Kwon B,Hong M

更新日期：2016-09-27 00:00:00

abstract：:To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants w...

journal_title：Biochemistry

authors： Snitko Y,Han SK,Lee BI,Cho W

更新日期：1999-06-15 00:00:00

abstract：:The active site topography of rabbit CYP4B1 has been studied relative to CYP2B1 and CYP102 using a variety of aromatic probe substrates. Oxidation of the prochiral substrate cumene by CYP4B1, but not CYP2B1 or CYP102, resulted in the formation of the thermodynamically disfavored omega-hydroxy metabolite, 2-phenyl-1-pr...

journal_title：Biochemistry

authors： Henne KR,Fisher MB,Iyer KR,Lang DH,Trager WF,Rettie AE

更新日期：2001-07-24 00:00:00

abstract：:FKBP12, a small human enzyme, aids protein folding by catalyzing cis-trans isomerization of peptidyl-prolyl bonds, and is involved in cell signaling pathways, calcium regulation, and the immune response. The underlying molecular mechanisms are not fully understood, but it is well-known that aromatic residues in the ac...

journal_title：Biochemistry

authors： Weininger U,Modig K,Geitner AJ,Schmidpeter PA,Koch JR,Akke M

更新日期：2017-01-10 00:00:00

abstract：:A grand canonical formalism is developed to combine discrete simulations for chemically distinct species in equilibrium. Each simulation is based on a perturbed funneled landscape. The formalism is illustrated using the alkaline-induced transitions of cytochrome c as observed by FTIR spectroscopy and with various othe...

journal_title：Biochemistry

authors： Weinkam P,Romesberg FE,Wolynes PG

更新日期：2009-03-24 00:00:00

abstract：:Living cells contain a range of densely phosphorylated surfaces, including phospholipid membranes, ribonucleoproteins, and nucleic acid polymers. Hyperphosphorylated surfaces also accumulate in neurodegenerative diseases as neurofibrillar tangles. We have synthesized and structurally characterized a precisely patterne...

journal_title：Biochemistry

authors： Li S,Sidorov AN,Mehta AK,Bisignano AJ,Das D,Childers WS,Schuler E,Jiang Z,Orlando TM,Berland K,Lynn DG

更新日期：2014-07-08 00:00:00

abstract：:Twenty-five different temperature-sensitive point mutations at 20 sites in the lysozyme gene of bacteriophage T4 have been identified. All of the mutations alter amino acid side chains that have lower than average crystallographic thermal factors and reduced solvent accessibility in the folded protein. This suggests t...

journal_title：Biochemistry

authors： Alber T,Sun DP,Nye JA,Muchmore DC,Matthews BW

更新日期：1987-06-30 00:00:00

abstract：:Animal galectins (lectins with specificity for beta-galactosides of glycan chains) are potent effectors in diverse aspects of cell sociology. Gene divergence has led to different groups and a marked interspecies variability in the number of members per group. Since the suitability of a model for studying functionality...

journal_title：Biochemistry

authors： Kaltner H,Solís D,André S,Lensch M,Manning JC,Mürnseer M,Sáiz JL,Gabius HJ

更新日期：2009-05-26 00:00:00

abstract：:In the primary quinone (Q(A)) binding site of Rb. sphaeroides reaction centers (RCs), isoleucine M265 is in extensive van der Waals contact with the ubiquinone headgroup. Substitution of threonine or serine for this residue (mutants M265IT and M265IS), but not valine (mutant M265IV), lowers the redox midpoint potentia...

journal_title：Biochemistry

authors： Wells TA,Takahashi E,Wraight CA

更新日期：2003-04-15 00:00:00

abstract：:The c-Jun N-terminal kinases (JNKs) play a wide variety of roles in cellular signaling processes, dictating important, and even divergent, cellular fates. These essential kinases possess docking surfaces distal to their active sites that interact with diverse binding partners, including upstream activators, downstream...

journal_title：Biochemistry

authors： Lombard CK,Davis AL,Inukai T,Maly DJ

更新日期：2018-10-09 00:00:00

abstract：:Adduct-induced DNA damage can affect transcription efficiency and DNA replication and repair. We previously investigated the effects of the 3'-next flanking base (G*CT vs G*CA; G*, FABP, N-(2'-deoxyguanosin-8-yl)-4'-fluoro-4-aminobiphenyl; FAF, N-(2'-deoxyguanosin-8-yl)-7-fluoro-2-aminofluorene) on the conformation of...

journal_title：Biochemistry

authors： Jain V,Vaidyanathan VG,Patnaik S,Gopal S,Cho BP

更新日期：2014-06-24 00:00:00

abstract：:Alpha-synuclein plays a key role in the pathogenesis of many neurodegenerative diseases. To date, its cellular role has yet to be determined, although it has been proposed to be connected to calcium and G protein-mediated dopamine signaling. Alpha-synuclein is known to bind strongly to model membrane surfaces where it...

journal_title：Biochemistry

authors： Narayanan V,Guo Y,Scarlata S

更新日期：2005-01-18 00:00:00

abstract：:The structure of the specific phosphate binding loop (P-loop) of bovine protein tyrosine phosphatase (BPTP) is very similar to that present in high M(r) PTPases. Site-directed mutagenesis was used to explore the role of several conserved residues involved in forming the P-loop of BPTP. Thus, Ser-19 and Ser-43 were ind...

journal_title：Biochemistry

authors： Evans B,Tishmack PA,Pokalsky C,Zhang M,Van Etten RL

更新日期：1996-10-22 00:00:00