Abstract:
:The differential scanning calorimetry trace of F1ATPase, prepared from beef heart submitochondrial particles, has a single sharp endothermic transition at 80.5 +/- 1.0 degrees C and a half-height peak width of 2.0 +/- 0.2 degrees. The transition enthalpy is 19 +/- 2 cal/g of protein. Submitochondrial particles (SMP) have a similar peak at 75.1 +/- 0.5 degrees C with a half-height peak width of 1.8 +/- 0.1 degrees and an enthalpy of 5 +/- 1 cal/g of SMP protein. The SMP transition is provisionally identified as being due to membrane-bound F1ATPase. Tetracaine and dibucaine cause these transitions to shift to lower temperatures; addition of 0.3 mM dibucaine gives peaks at 71.7 and 64.9 degrees C for F1ATPase and SMP, respectively, and 1.0 mM tetracaine gives peaks at 70.0 and 60.5 degrees C for F1ATPase and SMP, respectively. These anesthetic concentrations also give appreciable inhibition of enzyme activity at 25 degrees C. We conclude that the local anesthetics induce conformational alterations in the F1ATPase-protein complex which result both in enzyme inhibition and in the lowering of the thermal denaturation transition temperature.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Kresheck GC,Adade AB,Vanderkooi Gdoi
10.1021/bi00328a022subject
Has Abstractpub_date
1985-03-26 00:00:00pages
1715-9issue
7eissn
0006-2960issn
1520-4995journal_volume
24pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Characterization of the complete gene sequence encoding the alpha-galactosidase from Phanerochaete chrysosporium confirms that this enzyme is a member of glycosyl hydrolase family 27 [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. This family, together with the family 36 alpha-galactosidases, forms g...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0008074
更新日期:2000-08-15 00:00:00
abstract::Recently, we described the formation of a complex between liposomes and the unfolded protein rhodanese (thiosulfate:cyanide sulfurtransferase, EC 2.8.1.1), which could be liberated and efficiently reactivated after treatment of the complex with detergents [Zardeneta, G., & Horowitz, P. M. (1992) Eur. J. Biochem. 210, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00213a025
更新日期:1993-12-21 00:00:00
abstract::The reverse transcriptase (RT) from the human immunodeficiency virus (HIV) is initially expressed as a 66-kDa protein and is subsequently proteolytically processed in vivo to form a 66-kDa/51-kDa heterodimer. Comparison of circular dichroism spectra of the 66-kDa, 51-kDa, and heterodimeric forms of RT indicates that t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00150a015
更新日期:1992-09-08 00:00:00
abstract::The understanding of interactions between small molecules and DNA is crucial to design new anticancer drugs targeted to DNA. Methylene blue (MB) is a phenothiazinium dye that has shown promising results in photodynamic therapy treatment. The noncovalent binding of methylene blue to DNA was experimentally and theoretic...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi500068z
更新日期:2014-04-15 00:00:00
abstract::Ubiquitin was modified with the anionic, arginine-specific reagent 4-(oxoacetyl)phenoxyacetic acid in order to study the relationship between structure and function of the molecule. Four different derivatives (A, B, C, and D) were purified from the reaction mixture by anion-exchange high-performance liquid chromatogra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00396a019
更新日期:1987-11-03 00:00:00
abstract::Indolotryptoline natural products represent a small family of structurally unique chromopyrrolic acid-derived antiproliferative agents. Like many prospective anticancer agents before them, the exploration of their potential clinical utility has been hindered by the limited information known about their mechanism of ac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi501078j
更新日期:2014-11-18 00:00:00
abstract::Intrinsic factor (IF(50)) is a cobalamin (Cbl)-transporting protein of 50 kDa, which can be cleaved into two fragments: the 30 kDa N-terminal peptide IF(30) and the 20 kDa C-terminal glycopeptide IF(20). Experiments on binding of Cbl to IF(30), IF(20), and IF(50) revealed comparable association rate constants (k(+)(Cb...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047936v
更新日期:2005-03-08 00:00:00
abstract::The occupancy of the central cavity of the membrane-embedded c ring of the ATP synthase of Escherichia coli was investigated with a photo-cross-linking approach. Single cysteine mutants were created at c subunit positions 4, 8, and 11, which are oriented to the inside of the ring. These cysteines were alkylated with r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi052304+
更新日期:2006-02-14 00:00:00
abstract::The role of the protein shell in the formation of the hydrous ferric oxide core of ferritin is poorly understood. A VO2+ spin probe study was undertaken to characterize the initial complex of Fe2+ with horse spleen apoferritin (96% L-subunits). A competitive binding study of VO2+ and Fe2+ showed that the two metals co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00102a012
更新日期:1991-09-24 00:00:00
abstract::The hydration of CO2 catalyzed by carbonic anhydrase requires proton transfer from the zinc-bound water at the active site to solution for each cycle of catalysis. In the most efficient of the mammalian carbonic anhydrases, isozyme II, this transfer is facilitated by a proton shuttle residue, His 64. Murine carbonic a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9729209
更新日期:1998-05-19 00:00:00
abstract::Proteogenomic identification of translated small open reading frames in humans has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many reg...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.0c00672
更新日期:2020-10-27 00:00:00
abstract::A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9914964
更新日期:2000-01-11 00:00:00
abstract::Cations often deform the structure of regulatory proteins to affect a functional response, but for other protein functions a more passive effect is desired. For instance, it is shown here that in the conductance of Na+ by the gramicidin channel there appears to be no significant structural deformation of either the si...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961170k
更新日期:1996-09-17 00:00:00
abstract::Using solution-state NMR spectroscopy, new insights into the early events governing amelogenin supramolecular self-assembly have been identified using sodium chloride and calcium chloride to trigger the association. Two-dimensional 1H-15N HSQC spectra were recorded for 15N- and 13C-labeled murine amelogenin as a funct...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi8018288
更新日期:2008-12-16 00:00:00
abstract::Human S100B(beta beta) is a small intracellular EF-hand calcium-binding protein that consists of two noncovalently associated 91-residue beta monomers. The three-dimensional structures of S100B reveal the dimer interface consists of four alpha-helices (I, I' and IV, IV') packed in an X-type bundle. In this study, guan...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0118052
更新日期:2002-03-19 00:00:00
abstract::Catalysis by glycogen phosphorylase involves a mechanism in which binding of one substrate tightens the binding of the other substrate to produce a productive ternary enzyme-substrate complex. In this work the molecular basis for this synergism is probed in crystallographic studies on ternary complexes in which the gl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi960072w
更新日期:1996-06-11 00:00:00
abstract::Cytochrome P450 158A2 (CYP158A2) has been shown to catalyze an unusual oxidative C-C coupling reaction to polymerize flaviolin and form highly conjugated pigments (three isomers of biflaviolin and one triflaviolin) in Streptomyces coelicolor A3(2) which protect the soil bacterium from deleterious effects of UV irradia...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7006959
更新日期:2007-07-31 00:00:00
abstract::Derivatives of imidazole-2-thiones block reactions catalyzed by thyroid peroxidase (TPX) and the closely related lactoperoxidase (LPX), and this property is used therapeutically to treat hyperthyroidism. The reactions of a series of benzimidazoline-2-thiones with chemical and enzymatic oxidants were investigated to pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00052a009
更新日期:1993-01-12 00:00:00
abstract::The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF-50 binding. The crystal structure of the B...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801115g
更新日期:2008-11-04 00:00:00
abstract::The fluorescence polarization intensities from fluorescent probes and the electron paramagnetic resonance spectra from spin probes, specifically modifying elements of a biological assembly such as myosin sulfhydryl 1 (SH1) in muscle fibers, are interpreted in terms of probe order parameters using a model-independent m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00116a029
更新日期:1992-01-14 00:00:00
abstract::p-Hydroxyphenylpyruvate dioxygenase (HPD) plays a key role in the normal catabolism of tyrosine. An Fe2+/oxygen-dependent enzyme, it converts p-hydroxyphenylpyruvate into homogentisate and is part of the superfamily of alpha-ketoglutarate-dependent enzymes that couples oxidative decarboxylation of an alpha-ketoacid co...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035762w
更新日期:2004-01-27 00:00:00
abstract::Osteopontin (OPN) is an acidic hydrophilic glycophosphoprotein that was first identified as a major sialoprotein in bones. It functions as a cell attachment protein displaying a RGD cell adhesion sequence and as a cytokine that signals through integrin and CD44 cell adhesion molecules. OPN is also implicated in human ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi200291e
更新日期:2011-07-12 00:00:00
abstract::Doublet signal and singlet-like signal induced in Ca(2+)-depleted PS II were investigated by pulsed EPR in one-dimensionally oriented photosystem (PS) II membranes. The doublet signal showed marked angular dependent change in its spectrum in term of the applied magnetic field, indicating that the magnetic dipole-dipol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9929406
更新日期:2000-09-12 00:00:00
abstract::A single histidine residue has been placed at either the N-terminus or the C-terminus of each of the two alpha-helices of barnase. The pKa of that histidine residue in each of the four mutants has been determined by 1H NMR. The pKas of the two residues at the C-terminus are, on average, 0.5 unit higher, and those of t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00123a006
更新日期:1992-03-03 00:00:00
abstract::The relationship between triacylglycerol and monoacylglycerol hydrolyzing activities of purified rat heart lipoprotein lipase was studied using emulsified trioleoylglycerol and micellar or albumin-bound monooleoylglycerol as substrates. The maximal reaction rates obtained with the two substrates were similar (650 and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00654a017
更新日期:1976-05-04 00:00:00
abstract::Plasma kallikrein (Fletcher factor) is a hepatic serine proteinase that participated in the early phase of blood coagulation. From two genomic libraries, we succeeded to isolate four overlapping clones representing the entire rat plasma kallikrein gene. Using selective DNA sequencing, polymerase chain reactions, and r...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00220a027
更新日期:1991-02-12 00:00:00
abstract::NAD(P) aldehyde dehydrogenases (EC 1.2.1.3) are a family of enzymes that oxidize a wide variety of aldehydes into acid or activated acid compounds. Using site-directed mutagenesis, the essential nucleophilic Cys 149 in the NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli has...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00164a033
更新日期:1992-12-15 00:00:00
abstract::Decarboxylation reactions from comparable thiamin diphosphate- and thiamin-derived adducts of p-(halomethyl)benzoylformic acids in enzymic and non-enzymic reactions, respectively, reveal critical distinctions in otherwise similar Breslow intermediates. The ratio of protonation to chloride elimination from the Breslow ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00298
更新日期:2019-08-27 00:00:00
abstract::A metastable intermediate was found in the course of the denaturation of purified cytochrome P450 2B1 by increasing concentrations of guanidine hydrochloride (GuHCl). The metastable intermediate has no or low absorbance at 450 nm in the reduced carbon monoxide difference spectrum and has no absorbance at 420 nm. The i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00016a023
更新日期:1995-04-25 00:00:00
abstract::The cytoplasmic disassembly of Ran.GTP.importin and Ran.GTP.exportin. cargo complexes is an essential step in the corresponding nuclear import and export cycles. It has previously been shown that such disassembly can be mediated by RanBP1 in the presence of RanGAP. The nuclear pore complex protein RanBP2 (Nup358) cont...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001010f
更新日期:2000-09-26 00:00:00