Role of divalent cations in the 3',5'-exonuclease reaction of DNA polymerase I.

abstract：:We show that eight different serine proteinases--bovine chymotrypsins A and B, porcine pancreatic elastase I, proteinase K, Streptomyces griseus proteinases A and B, and subtilisins BPN' and Carlsberg--interact with turkey ovomucoid third domain at the same Leu18-Glu19 peptide bond, the reactive site of the inhibitor....

journal_title：Biochemistry

authors： Ardelt W,Laskowski M Jr

更新日期：1985-09-24 00:00:00

abstract：:Intramolecular excimerization of 1,3-di-1-pyrenylpropane [Py(3)Py] was used to assess the fluidity of sarcoplasmic reticulum membranes (SR); on the basis of the spectral data, the probe incorporates completely inside the membrane probably somewhere close to the polar head groups of phospholipid molecules, however not ...

journal_title：Biochemistry

authors： Almeida LM,Vaz WL,Zachariasse KA,Madeira VM

更新日期：1984-09-25 00:00:00

abstract：:Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemi...

journal_title：Biochemistry

authors： Wichmann JK,Schnoes HK,DeLuca HF

更新日期：1981-12-22 00:00:00

abstract：:Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...

journal_title：Biochemistry

authors： Willis LJ,Loehr TM

更新日期：1985-05-21 00:00:00

abstract：:The pH dependence of the transient aerobic kinetics of cytochromes c and a has been investigated with cytochrome oxidase reconstituted in phospholipid vesicles in the absence and presence of an uncoupler and an ionophore. The cytochrome a reduction level immediately after the burst phase was 60-80% and was not signifi...

journal_title：Biochemistry

authors： Maison-Peteri B,Malmström BG

更新日期：1989-04-18 00:00:00

abstract：:Cathepsin K is a cysteine protease present in human osteoclasts that plays an important role in bone resorption. Cathepsin K is synthesized as an inactive proenzyme and activated under conditions of low pH. Autoproteolytic processing of the N-terminal 99 amino acid propeptide produces the active, mature form of cathep...

journal_title：Biochemistry

authors： LaLonde JM,Zhao B,Janson CA,D'Alessio KJ,McQueney MS,Orsini MJ,Debouck CM,Smith WW

更新日期：1999-01-19 00:00:00

abstract：:The membrane-bound closed channel structure of helices 4 and 5 (Lys-406-Asp-446) of the colicin E1 channel domain was investigated by using a site-directed fluorescence labeling technique. A bimane probe was covalently attached to a cysteine residue in a series of single-cysteine mutant proteins to scan each helix in ...

journal_title：Biochemistry

authors： Ho D,Merrill AR

更新日期：2009-02-17 00:00:00

abstract：:The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...

journal_title：Biochemistry

authors： Komatsu H,Chong PL

更新日期：1998-01-06 00:00:00

abstract：:We have undertaken ultrafast infrared (IR) spectroscopic studies in order to elucidate the geometry of bound CO in the alpha and beta subunits of hemoglobin (Hb) M Boston 13CO. Hb M Boston is a mutant human Hb in which the distal histidine in the alpha subunits is replaced by a tyrosine. The IR absorptions of bound 13...

journal_title：Biochemistry

authors： Lian T,Locke B,Kitagawa T,Nagai M,Hochstrasser RM

更新日期：1993-06-08 00:00:00

abstract：:Nuclear magnetic resonance (NMR) (15)N relaxation methods have been used to characterize the backbone dynamics of the N-terminal core domain of the HIV-1 capsid protein (CA(151)). The domain, which has an unusually flat, triangular shape, tumbles in solution at 28 degrees C with an effective rotational correlation tim...

journal_title：Biochemistry

authors： Campos-Olivas R,Summers MF

更新日期：1999-08-10 00:00:00

abstract：:Hydrogen exchange of the individual amide protons of alanine-90 (F5), glutamine-91 (F6), serine-92 (F7), and histidine-93 (F8) residues in cyanometmyoglobin of sperm whale has been studied by 1H nuclear magnetic resonance spectroscopy at 360 MHz. The amide proton resonance of F5, F6, and F7 have been assigned by use o...

journal_title：Biochemistry

authors： Kumar NV,Kallenbach NR

更新日期：1985-12-17 00:00:00

abstract：:The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has been reversibly unfolded in a protein/detergent/mixed micelle system by varying the molar ratio of n-decyl beta-D-maltoside (DM) and sodium dodecyl sulfate (SDS). Unfolding was monitored by circular dichroism (CD) and ultraviolet (UV) ...

journal_title：Biochemistry

authors： Lau FW,Bowie JU

更新日期：1997-05-13 00:00:00

abstract：:It has been shown by genetic analysis that the external and internal invertases from Saccharomyces cerevisiae share a common structural gene [Taussig, R., & Carlson, M. (1983) Nucleic Acids Res. 11, 1943-1954]. However, the only amino acid composition of these two forms of invertase reported to date has revealed exten...

journal_title：Biochemistry

authors： Chu FK,Takase K,Guarino D,Maley F

更新日期：1985-10-22 00:00:00

abstract：:Oxalate:ferredoxin oxidoreductase (OOR) is an unusual member of the thiamine pyrophosphate (TPP)-dependent 2-oxoacid:ferredoxin oxidoreductase (OFOR) family in that it catalyzes the coenzyme A (CoA)-independent conversion of oxalate into 2 equivalents of carbon dioxide. This reaction is surprising because binding of C...

journal_title：Biochemistry

authors： Pierce E,Mansoorabadi SO,Can M,Reed GH,Ragsdale SW

更新日期：2017-06-06 00:00:00

abstract：:Crk-II is a signaling adaptor protein that is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an Src homology 2 domain (SH2) followed by two Src homology 3 (SH3) domains. The structures and ligand-binding properties of the ...

journal_title：Biochemistry

authors： Muralidharan V,Dutta K,Cho J,Vila-Perello M,Raleigh DP,Cowburn D,Muir TW

更新日期：2006-07-25 00:00:00

abstract：:Polylysine promoted extensive membrane mixing of liposomes only if the buffer pH was below the pKa of the lysyl residues. This observation suggested that fusion could be regulated in a physiological pH range if the homopolymer of L-histidine was substituted as fusogen. Microgram quantities of polyhistidine were added ...

journal_title：Biochemistry

authors： Uster PS,Deamer DW

更新日期：1985-01-01 00:00:00

abstract：:We have investigated the loss of histone H1 from chromatin [D'Anna, J. A., Gurley, L. R., & Tobey, R. A. (1982) Biochemistry 21, 3991-4001] and the structure of chromatin from Chinese hamster (line CHO) cells blocked in early S phase by sequential use of isoleucine deprivation G1 block and 1 mM hydroxyurea (HU) blocka...

journal_title：Biochemistry

authors： D'Anna JA,Prentice DA

更新日期：1983-11-22 00:00:00

abstract：:Homeodomain-only protein (HOP) is an 8-kDa transcriptional corepressor that is essential for the normal development of the mammalian heart. Previous studies have shown that HOP, which consists entirely of a putative homeodomain, acts downstream of Nkx2.5 and associates with the serum response factor (SRF), repressing ...

journal_title：Biochemistry

authors： Kook H,Yung WW,Simpson RJ,Kee HJ,Shin S,Lowry JA,Loughlin FE,Yin Z,Epstein JA,Mackay JP

更新日期：2006-09-05 00:00:00

abstract：:Transition metal-transporting P1B-type CPx ATPases play crucial roles in mediating metal homeostasis and resistance in all cells. The degree to which N-terminal metal binding domains (MBDs) confer metal specificity to the transporter is unclear. We show that the two MBDs of the Zn/Cd/Pb effluxing pump Anabaena AztA ar...

journal_title：Biochemistry

authors： Liu T,Reyes-Caballero H,Li C,Scott RA,Giedroc DP

更新日期：2007-10-02 00:00:00

abstract：:The mechanism of the oxidative decarboxylation reaction catalyzed by the NAD-malic enzyme from Ascaris suum has been examined with several different divalent metal ion activators and dinucleotide substrates. Primary deuterium and tritium isotope effects have been obtained and, in combination with the partitioning rati...

journal_title：Biochemistry

authors： Karsten WE,Gavva SR,Park SH,Cook PF

更新日期：1995-03-14 00:00:00

abstract：:We have investigated the unfolding thermodynamics for incorporating cationic side chains in the Dickerson-Drew dodecamer duplex. Incorporation of two 3-aminopropyl-2'-deoxyuridine residues (one on each self-complementary strand) lowers the stability of the duplex. This reduction is driven by unfavorable heat contribut...

journal_title：Biochemistry

authors： Shikiya R,Li JS,Gold B,Marky LA

更新日期：2005-09-20 00:00:00

abstract：:Fusion of the human immunodeficiency virus (HIV) with target cells is mediated by the gp41 subunit of the envelope protein. Mutation and deletion studies within the transmembrane domain (TMD) of intact gp41 influenced its fusion activity. In addition, current models suggest that the TMD is in proximity with the fusion...

journal_title：Biochemistry

authors： Reuven EM,Dadon Y,Viard M,Manukovsky N,Blumenthal R,Shai Y

更新日期：2012-04-03 00:00:00

abstract：:Nucleolin, also called protein C23, is a RNA-associated protein implicated in the early stages of ribosome assembly. To study the general conformation and map the nucleic acid binding regions, rat nucleolin was subjected to limited proteolysis using trypsin and chymotrypsin in the presence or absence of poly(G). The c...

journal_title：Biochemistry

authors： Olson MO,Kirstein MN,Wallace MO

更新日期：1990-06-19 00:00:00

abstract：:The binding of 8-anilino-1-naphthalenesulfonate (ANS) to ciliary dynein ATPase leads to a marked increase in the dye's fluorescence intensity, accompanied by a blue shift in the observed fluorescence emission maximum. We found that dynein has 37 +/- 3 ANS binding sites and that experimentally applied ANS concentration...

journal_title：Biochemistry

authors： Saucier AC,Mariotti S,Anderson SA,Purich DL

更新日期：1985-12-17 00:00:00

abstract：:The enzyme thymidylate synthase (TS) catalyzes a complex reaction that involves forming and breaking at least six covalent bonds. The physical nature of the hydride transfer step in this complex reaction cascade has been studied by means of isotope effects and their temperature dependence. Competitive kinetic isotope ...

journal_title：Biochemistry

authors： Agrawal N,Hong B,Mihai C,Kohen A

更新日期：2004-02-24 00:00:00

abstract：:The synthetic peptide SNX-111 corresponding to the sequence of the omega-conopeptide MVIIA from the venom of the marine snail Conus magus is a highly potent and selective antagonist of N-type calcium channels. We have synthesized and characterized a large number of analogs of SNX-111 in order to elucidate the structur...

journal_title：Biochemistry

authors： Nadasdi L,Yamashiro D,Chung D,Tarczy-Hornoch K,Adriaenssens P,Ramachandran J

更新日期：1995-06-27 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:The influence of structural alterations to the estradiol-17 beta (E2) molecule on the induction of pS2 and Cathepsin D (Cath D) mRNAs has been examined by Northern analysis of RNA extracted from MCF-7 cells. Exposure of cultures to estratriene did not affect the level of expression of these estrogen-responsive genes. ...

journal_title：Biochemistry

authors： Pilat MJ,Hafner MS,Kral LG,Brooks SC

更新日期：1993-07-13 00:00:00

abstract：:The Met16Phe mutant of the type 1 copper protein pseudoazurin (PACu), in which a phenyl ring is introduced close to the imidazole moiety of the His81 ligand, has been characterized. NMR studies indicate that the introduced phenyl ring is parallel to the imidazole group of His81. The mutation has a subtle effect on the...

journal_title：Biochemistry

authors： Yanagisawa S,Sato K,Kikuchi M,Kohzuma T,Dennison C

更新日期：2003-06-10 00:00:00

abstract：:Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are devoid of catalytic activity, they are still highly specific myotoxins and have recently been sho...

journal_title：Biochemistry

authors： Pedersen JZ,Lomonte B,Massoud R,Gubensek F,Gutiérrez JM,Rufini S

更新日期：1995-04-11 00:00:00