Role of divalent cations in the 3',5'-exonuclease reaction of DNA polymerase I.

abstract：:Cysteinyl S-nitrosylation has emerged as an important post-translational modification affecting protein function in health and disease. Great emphasis has been placed on global, unbiased quantification of S-nitrosylated proteins because of physiologic and oxidative stimuli. However, current strategies have been hamper...

journal_title：Biochemistry

authors： Wiktorowicz JE,Stafford S,Rea H,Urvil P,Soman K,Kurosky A,Perez-Polo JR,Savidge TC

更新日期：2011-06-28 00:00:00

abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...

journal_title：Biochemistry

authors： Jung JH,Pendergast AM,Zipfel PA,Traugh JA

更新日期：2008-01-22 00:00:00

abstract：:Cyclobutane pyrimidine dimers (Pyr < > Pyr) are the major DNA photoproducts induced by the UV component of solar radiation. Photoreactivating enzyme (DNA photolyase) repairs DNA by utilizing the energy of visible light to break the cyclobutane ring of the dimer. Photolyases are monomeric proteins of 50-60 kDa with sto...

journal_title：Biochemistry

authors： Sancar A

更新日期：1994-01-11 00:00:00

abstract：:The (beta alpha)8 (or "TIM")-barrel protein phosphoribosylanthranilate isomerase from Saccharomyces cerevisiae was cleaved between the sixth and seventh beta alpha module to test the capacity of the resulting fragments to adopt native format autonomously. The fragments, which were expressed from separate coding sequen...

journal_title：Biochemistry

authors： Eder J,Kirschner K

更新日期：1992-04-14 00:00:00

abstract：:The enzymes in the catechol meta-fission pathway have been studied for more than 50 years in several species of bacteria capable of degrading a number of aromatic compounds. In a related pathway, naphthalene, a toxic polycyclic aromatic hydrocarbon, is fully degraded to intermediates of the tricarboxylic acid cycle by...

journal_title：Biochemistry

authors： Guimarães SL,Coitinho JB,Costa DM,Araújo SS,Whitman CP,Nagem RA

更新日期：2016-05-10 00:00:00

abstract：:One- and two-dimensional NMR experiments were carried out on a decamer, d-(CGCTTTTCGC).d(GCGAAAAGCG), and on the same sequence with the addition of an unpaired thymidine, d(CGCTTTTCGC).d(GCGAATAAGCG), which will be referred to as the T-bulge decamer. Evidence from one-dimensional NOE experiments on the exchangeable pr...

journal_title：Biochemistry

authors： Morden KM,Gunn BM,Maskos K

更新日期：1990-09-18 00:00:00

abstract：:Aromatic residues in c-type cytochromes might have an important function in the folding and/or electron transferring properties of the molecule. In the tetraheme cytochrome c3 (Mr 13 000) from Desulfovibrio vulgaris Hildenborough, Phe20, is located between heme 1 and heme 3 with its aromatic ring close and almost para...

journal_title：Biochemistry

authors： Dolla A,Arnoux P,Protasevich I,Lobachov V,Brugna M,Giudici-Orticoni MT,Haser R,Czjzek M,Makarov A,Bruschi M

更新日期：1999-01-05 00:00:00

abstract：:The hammerhead ribozyme undergoes a well-defined two-stage conformational folding process, induced by the binding of magnesium ions. In this study, we have used isothermal titration calorimetry to analyze the thermodynamics of magnesium binding and magnesium ion-induced folding of the ribozyme. Binding to the natural ...

journal_title：Biochemistry

authors： Hammann C,Cooper A,Lilley DM

更新日期：2001-02-06 00:00:00

abstract：:The toll that Streptococcus pneumoniae exacts on the welfare of humanity is enormous. This organism claims the lives of approximately 3700 people daily, the majority of whom are children below the age of 5, and the situation could worsen due to the increasing incidence of pernicious, multiple-antibiotic-resistant stra...

journal_title：Biochemistry

authors： Andreassi JL 2nd,Dabovic K,Leyh TS

更新日期：2004-12-28 00:00:00

abstract：:Cytochrome c has been chemically modified by methylene blue mediated photooxidation. It is established that the methionine residues of the protein have been specifically converted to methionine sulfoxide residues. No oxidation of any other amino acid residues or the cysteine thioether bridges of the molecule occurs du...

journal_title：Biochemistry

authors： Ivanetich KM,Bradshaw JJ,Kaminsky LS

更新日期：1976-03-09 00:00:00

abstract：:Although the mechanism by which the steroidogenic acute regulatory protein (StAR) promotes steroidogenesis has been studied extensively, it remains incompletely characterized. Because structural analysis has revealed a hydrophobic sterol-binding pocket (SBP) within StAR, this study sought to examine the regulatory rol...

journal_title：Biochemistry

authors： Rajapaksha M,Kaur J,Bose M,Whittal RM,Bose HS

更新日期：2013-10-15 00:00:00

abstract：:The anomeric proton (H-1) chemical shifts of D-mannopyranosides in aqueous solution are affected both by the aglycon and by substitution of the ring [Lee, Y. C., & Ballou, C. E. (1965) Biochemistry 4, 257]. We have examined the 1H NMR spectra for a variety of linear and branched mannooligosaccharides and have assigned...

journal_title：Biochemistry

authors： Cohen RE,Ballou CE

更新日期：1980-09-02 00:00:00

abstract：:The crystal structure of the particulate methane monooxygenase (pMMO) from Methylococcus capsulatus (Bath) has been reported recently [Lieberman, R. L., and Rosenzweig, A. C. (2005) Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane, Nature 434, 177-182]. Subsequent ...

journal_title：Biochemistry

authors： Yu SS,Ji CZ,Wu YP,Lee TL,Lai CH,Lin SC,Yang ZL,Wang VC,Chen KH,Chan SI

更新日期：2007-12-04 00:00:00

abstract：:The oxygen enzymatically inserted as a hydroxyl function by chick kidney homogenate into the 24 position of 25-hydroxyvitamin D3 to give 24,25-dihydroxyvitamin D3 is derived exclusively from 18O2. Therefore, like the 25-hydroxyvitamin D3-lalpha-hydroxylase system, the 25-hydroxyvitamin D3-24-hydroxylase system is also...

journal_title：Biochemistry

authors： Madhok TC,Schnoes HK,DeLuca HF

更新日期：1977-05-17 00:00:00

abstract：:Solutions of porcine lactate dehydrogenase of micromolar concentration kept at 4 degrees C for several days lose the greater part of their enzymic activity but recover it when returned to room temperature. The rate of spoiling decreases and the rate of recovery increases with the concentration of the solutions. The de...

journal_title：Biochemistry

authors： King L,Weber G

更新日期：1986-06-17 00:00:00

abstract：:A study of the voltage induction of transient pores in phospholipid bilayer vesicles is reported. Unilamellar vesicles (dipalmitoylphosphatidylcholine), with a size distribution of 100 +/- 30 nm, were prepared by the method of Enoch & Strittmatter [Enoch, H., & Strittmatter, P. (1979) Proc. Natl. Acad. Sci. U.S.A. 76,...

journal_title：Biochemistry

authors： Teissie J,Tsong TY

更新日期：1981-03-17 00:00:00

abstract：:The chromatin-associated high-mobility group (HMG) proteins of the plant HMGB family are characterized by a central HMG-box domain that is flanked by a basic N-terminal and an acidic C-terminal domain. By functional interaction with certain transcription factors, HMGB proteins contribute to transcriptional regulation....

journal_title：Biochemistry

authors： Grasser M,Christensen JM,Peterhänsel C,Grasser KD

更新日期：2007-05-29 00:00:00

abstract：:Three crystal structures have been determined of active site specific substituted Cd(II) horse liver alcohol dehydrogenase and its complexes. Intensities were collected for the free, orthorhombic enzyme to 2.4-A resolution and for a triclinic binary complex with NADH to 2.7-A resolution. A ternary complex was crystall...

journal_title：Biochemistry

authors： Schneider G,Cedergren-Zeppezauer E,Knight S,Eklund H,Zeppezauer M

更新日期：1985-12-03 00:00:00

abstract：:Double-stranded complementary deoxyribonucleic acid (cDNA) transcribed in vitro from a partially pure myosin light-chain messenger ribonucleic acid (mRNA) of the chick embryonic heart was cloned in Escherichia coli strain chi 1776 by using the HindIII cleavage site in the plasmid pBR322. The insertion of essentially f...

journal_title：Biochemistry

authors： Arnold HH,Siddiqui MA

更新日期：1979-12-11 00:00:00

abstract：:Synthetic ligands containing up to six residues of nonreducing terminal galactose were prepared. The synthesis involved coupling of carboxyl groups of N-benzyloxy-carbonylaspartic acid or of N-benzyloxycarbonyltyrosyl-gamma-glutamylglutamic acid to the omega-amino group of the aglycon of a glycoside that contained up ...

journal_title：Biochemistry

authors： Lee RT,Lin P,Lee YC

更新日期：1984-08-28 00:00:00

abstract：:Bacterial resistance to the aminoglycoside antibiotics is manifested primarily through the production of enzymes which covalently modify these drugs. The Enterococci and Staphylococci produce an ATP-dependent kinase, APH(3')-IIIa, which phosphorylates such antibiotics as kanamycin, amikacin, and neomycin, and this enz...

journal_title：Biochemistry

authors： McKay GA,Wright GD

更新日期：1996-07-02 00:00:00

abstract：:Hemopexin is a plasma glycoprotein that has two structural domains (I and II) and binds and transports heme particularly to liver cells. Differential scanning calorimetry (DSC) studies show that hemopexin is largely stabilized by heme, which binds exclusively to domain I. The melting temperature (Tm) of heme-hemopexin...

journal_title：Biochemistry

authors： Wu ML,Morgan WT

更新日期：1993-07-20 00:00:00

abstract：:Three sites of N(G),N(G)-arginine methylation have been located at residues 205, 217, and 224 in the glycine-rich, COOH-terminal one-third of the HeLa A1 heterogeneous ribonucleoprotein. Together with the previously determined dimethylated arginine at position 193 [Williams et al., (1985) Proc. Natl. Acad. Sci. U.S.A....

journal_title：Biochemistry

authors： Kim S,Merrill BM,Rajpurohit R,Kumar A,Stone KL,Papov VV,Schneiders JM,Szer W,Wilson SH,Paik WK,Williams KR

更新日期：1997-04-29 00:00:00

abstract：:The enzyme form of the calcium adenosinetriphosphatase of sarcoplasmic reticulum (CaATPase) that is stable in the presence of calcium, cE.Ca2, has a binding site for the catalytic Mg2+ ion with a dissociation constant of 0.94 +/- 0.15 mM at 25 degrees C, pH 7.0, and 100 mM KCl. This is approximately 10 times smaller t...

journal_title：Biochemistry

authors： Reinstein J,Jencks WP

更新日期：1993-07-06 00:00:00

abstract：:Sedimentation equilibrium and sedimentation velocity measurements demonstrate that turkey gizzard caldesmon is an elongated molecule of molecular mass 75 +/- 2 kDa. The frictional ratio (2.14) is consistent with a prolate ellipsoid of axial ratio 24, corresponding to an apparent length and width of 516 and 21.5 A, res...

journal_title：Biochemistry

authors： Malencik DA,Ausio J,Byles CE,Modrell B,Anderson SR

更新日期：1989-10-03 00:00:00

abstract：:In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EII(mtl), are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol...

journal_title：Biochemistry

authors： Vos EP,Bokhove M,Hesp BH,Broos J

更新日期：2009-06-16 00:00:00

abstract：:The light harvesting 1 antenna (LH1) complex from Rhodobacter sphaeroides funnels excitation energy to the photosynthetic reaction center. Our ultimate goal is to build up the structure of LH1 from structures of its individual subunits, much as the antenna can self-assemble from its components in membrane-mimicking de...

journal_title：Biochemistry

authors： Sorgen PL,Cahill SM,Krueger-Koplin RD,Krueger-Koplin ST,Schenck CC,Girvin ME

更新日期：2002-01-08 00:00:00

abstract：:The importance of DNA polymerase-DNA minor groove interactions on translesion synthesis (TLS) was examined in vitro using variants of exonuclease-deficient Klenow polymerase and site-specifically modified DNA oligonucleotides. Polymerase variant R668A lacks primer strand interactions, while variant Q849A lacks templat...

journal_title：Biochemistry

authors： Gestl EE,Eckert KA

更新日期：2005-05-10 00:00:00

abstract：:The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...

journal_title：Biochemistry

authors： Rheinnecker M,Baker G,Eder J,Fersht AR

更新日期：1993-02-09 00:00:00

abstract：:The filamentous coliphage M13 possesses multiple copies of a 50-residue coat protein which is inserted into the inner membrane of Escherichia coli during infection. 13C nuclear magnetic resonance (NMR) spectroscopy has been used to probe the structure and dynamics of M13 coat protein solubilized in detergent micelles....

journal_title：Biochemistry

authors： Henry GD,Weiner JH,Sykes BD

更新日期：1986-02-11 00:00:00