Abstract：
:Crk-II is a signaling adaptor protein that is involved in many cellular processes including apoptosis, proliferation, and differentiation. It has a modular domain architecture consisting of an Src homology 2 domain (SH2) followed by two Src homology 3 (SH3) domains. The structures and ligand-binding properties of the SH2 and the middle SH3 domains are well-characterized. Several studies suggest that the C-terminal SH3 domain plays an important regulatory role in the protein; however, no structural information is available on this domain, and relatively little is known about its binding partners. In the current work, we have solved the solution NMR structure of the C-terminal SH3 domain. The domain adopts the standard SH3 fold comprising a five-stranded beta barrel. In agreement with alignment and modeling studies, the structure indicates that the canonical-binding surface of the SH3 domain is unusually polar and suggests that this domain may not bind typical PXXP ligands or that it may bind them with reduced affinity. Thermodynamic and kinetic studies show that the domain folds in a reversible two-state manner and that the stability of the fold is similar to that observed for other SH3 domains. These studies offer some insight into the likely structural and thermodynamic consequences of point mutations in the cSH3 domain that are known to deregulate Crk-II function. Our results set the stage for a better understanding the role of the cSH3 domain in the context of the full-length protein.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Muralidharan V,Dutta K,Cho J,Vila-Perello M,Raleigh DP,Cowburn D,Muir TWdoi
10.1021/bi060590zsubject
Has Abstractpub_date
2006-07-25 00:00:00pages
8874-84issue
29eissn
0006-2960issn
1520-4995journal_volume
45pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00200a036
更新日期：1994-08-30 00:00:00
abstract：:The dynamics of the backbone and (some of) the side chains of lac headpiece (1-56; lac HP56) have been studied for the free protein and for its complex with lac half-operator DNA by 15N T1 and T1p relaxation measurements combined with [1H-15N] NOE experiments. For the structurally well-defined part of the free lac HP5...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961670d
更新日期：1997-01-07 00:00:00
abstract：:Protein kinase C-related kinases (PRKs) are serine/threonine kinases that are members of the protein kinase C superfamily and can be activated by binding to members of the Rho family of small G proteins via a Rho binding motif known as an HR1 domain. The PRKs contain three tandem HR1 domains at their N-termini. The st...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200039u
更新日期：2011-04-12 00:00:00
abstract：:UreE is a homodimeric metallo-chaperone that assists the insertion of Ni(2+) ions in the active site of urease. The crystal structures of UreE from Bacillus pasteurii and Klebsiella aerogenes have been determined, but the details of the nickel-binding site were not elucidated due to solid-state effects that caused dis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0601003
更新日期：2006-05-23 00:00:00
abstract：:The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi4005528
更新日期：2013-08-06 00:00:00
abstract：:Several common links between the structural chemistry of the chitolipooligosaccharides of Rhizobium and the general rhizobial membrane lipid and lipopolysaccharide chemistry of these bacteria have been uncovered. Aspects of common chemistry include sulfation, methylation, and the position and extent of fatty acyl chai...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00013a040
更新日期：1995-04-04 00:00:00
abstract：:The size and surface curvature dependence of the properties and stability of single lamellar vesicles have been investigated by using a variety of physicochemical techniques. Dipalmitoylphosphatidylcholine single lamellar vesicles of sizes ranging between 200 and 900 A in diameter have been prepared by the French pres...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00515a024
更新日期：1981-06-09 00:00:00
abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00371a069
更新日期：1986-11-18 00:00:00
abstract：:Conjugative transfer of F-like plasmids in Escherichia coli is repressed by a plasmid-encoded protein, FinO. FinO blocks the translation of TraJ, a positive activator of transcription of genes required for conjugation. FinO binds a traJ antisense RNA, FinP, thereby protecting it from degradation, and catalyzes FinP-tr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9911482
更新日期：1999-10-19 00:00:00
abstract：:The protease activity of hepatitis C virus nonstructural protein 3 (NS3) is essential for viral replication. ITMN-191, a macrocyclic inhibitor of the NS3 protease active site, promotes rapid, multilog viral load reductions in chronic HCV patients. Here, ITMN-191 is shown to be a potent inhibitor of NS3 with a two-step...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900038p
更新日期：2009-03-24 00:00:00
abstract：:In the absence of ligands, dihydrofolate reductase from Escherichia coli exists in at least two interconvertible conformations, only one of which binds NADPH with high affinity. This equilibrium is pH dependent, involving an ionizable group of the enzyme (pK approximately 5.5), and the proportion of the NADPH-binding ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00489a010
更新日期：1990-09-18 00:00:00
abstract：:Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acy...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980565w
更新日期：1998-08-25 00:00:00
abstract：:The fluorescence polarization intensities from fluorescent probes and the electron paramagnetic resonance spectra from spin probes, specifically modifying elements of a biological assembly such as myosin sulfhydryl 1 (SH1) in muscle fibers, are interpreted in terms of probe order parameters using a model-independent m...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00116a029
更新日期：1992-01-14 00:00:00
abstract：:The ligand-binding domain (LBD) of the human androgen receptor (hAR LBD), encompassing amino acids (AAs) 647-919, was expressed in Escherichia coli with an N-terminal polyhistidine tag (His(10)-hAR LBD) from a pET-16b vector. The overexpressed protein was initially insoluble in inclusion bodies, and was subsequently s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010525j
更新日期：2001-09-11 00:00:00
abstract：:Fibronectin (FN) is an extracellular matrix (ECM) protein found soluble in corporal fluids or as an insoluble fibrillar component incorporated in the ECM. This phenomenon implicates structural changes that expose FN binding sites and activate the protein to promote intermolecular interactions with other FN. We have in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0347104
更新日期：2004-02-17 00:00:00
abstract：:Solvent proton nuclear magnetic dispersion studies at 25, 100, and 300 MHz have been performed on protocatechuate 3,4-dioxygenase (PCD) and its complexes with 3-chloro-4-hydroxybenzoate and 3-fluoro-4-hydroxybenzoate. Longitudinal and transverse relaxation rates were measured for these compounds and for the apoenzyme....
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00312a024
更新日期：1984-08-14 00:00:00
abstract：:The reduction of CBrCl3 by the heme-heme oxygenase complex forms dissociable and covalently bound heme products. No such products are formed with mesoheme in which the heme vinyl substituents are replaced by ethyl groups. The dissociable heme products are chromatographically similar but not identical to those obtained...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972720x
更新日期：1998-03-03 00:00:00
abstract：:The electrons extracted from the CaMn(4) cluster during water oxidation in photosystem II are transferred to P(680)(+) via the redox-active tyrosine D1-Tyr161 (Y(Z)). Upon Y(Z) oxidation a proton moves in a hydrogen bond toward D1-His190 (His(Z)). The deprotonation and reprotonation mechanism of Y(Z)-OH/Y(Z)-O is of k...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101364t
更新日期：2010-11-16 00:00:00
abstract：:Rat liver sinusoidal endothelial cells (LECs) mediate the removal of hyaluronan (HA) from the circulation via a specific Ca(2+)-independent endocytic receptor. To characterize the receptor biochemically, detergent-soluble extracts were prepared from crude LEC membranes. Using a dot blot assay to quantitate 125I-HA bin...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00117a039
更新日期：1992-01-21 00:00:00
abstract：:The proximal bond between the iron atom of the heme group and the N epsilon of histidine F8 in myoglobin (Mb) and hemoglobin (Hb) is presumed to be an important determinant of heme binding, protein structure, and oxygen binding. Here a system is described in which the proximal ligand is provided intermolecularly by th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00187a023
更新日期：1994-05-31 00:00:00
abstract：:The purified ATP-synthesizing complex from chloroplasts has been reconstituted into phospholipid vesicles with bacteriorhodopsin by use of octyl glucoside. Phosphorylation rates up to 90 mmol of ATP (mg of protein)-1 min-1 have been achieved. The dependence of the steady-state kinetic parameters on external and intern...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00527a018
更新日期：1981-11-24 00:00:00
abstract：:Animal galectins (lectins with specificity for beta-galactosides of glycan chains) are potent effectors in diverse aspects of cell sociology. Gene divergence has led to different groups and a marked interspecies variability in the number of members per group. Since the suitability of a model for studying functionality...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900083q
更新日期：2009-05-26 00:00:00
abstract：:The vanadate cofactor in vanadium chloroperoxidase has been studied using UV-VIS absorption spectroscopy. A band is present in the near-UV that is red-shifted as compared to free vanadate and shifts in both position and intensity upon change in pH. Mutation of vanadate binding residues has a clear effect on the spectr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9921790
更新日期：2000-02-08 00:00:00
abstract：:The three-dimensional structure of the complex between calmodulin (CaM) and a peptide corresponding to the N-terminal portion of the CaM-binding domain of the plasma membrane calcium pump, the peptide C20W, has been solved by heteronuclear three-dimensional nuclear magnetic resonance (NMR) spectroscopy. The structure ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9908235
更新日期：1999-09-21 00:00:00
abstract：:We have studied the pathway and kinetics of refolding of recombinant human brain-derived neurotrophic factor (BDNF), which is a very tightly-associated dimer in its native state. When BDNF unfolded in 6 M guanidine hydrochloride is diluted 20-fold into phosphate-buffered saline, a partially folded intermediate is rapi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00091a036
更新日期：1993-10-12 00:00:00
abstract：:Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi901583n
更新日期：2010-01-26 00:00:00
abstract：:Low-temperature electronic absorption and room temperature circular dichroism (CD) and magnetic circular dichroism (MCD) spectra are reported for ascorbate oxidase. Bands attributable to d-d electronic transitions in the type 1 (blue) coppers (2B2 ground state) have been observed at 5800, 10000, and 12000 cm-1 (1725, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00510a044
更新日期：1981-03-31 00:00:00
abstract：:Many lipases are potent catalysts of stereoselective reactions and are therefore of interest for use in chemical synthesis. The crystal structures of lipases show a large variation in the shapes of their active site environments that may explain the large variation in substrate specificity of these enzymes. We have de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00051a035
更新日期：1995-12-26 00:00:00
abstract：:Cooperative binding of the bacteriophage lambda cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the phage. Cooperativity has long been thought to be mediated by self-assembly of repressor dimers to form tetramers which can bind simultaneously to adjacent operat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971368k
更新日期：1997-10-21 00:00:00
abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00566a019
更新日期：1980-12-09 00:00:00
