当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Phosphorylase phosphatase complex from skeletal muscle. Activation of one of two catalytic subunits by manganese ions.

Phosphorylase phosphatase complex from skeletal muscle. Activation of one of two catalytic subunits by manganese ions.

Abstract：

:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodium dodecyl sulfate gel electrophoresis. The phosphorylase phosphatase activity of the complex form was activated more than 10-fold by Mn2+, with a K0.5 of 10(-5) M, but not by Mg2+ or Ca2+. Manganese activation occurred over a period of several minutes and resulted primarily in an increase in Vmax of a phosphatase that was sensitive to trypsin. Activation persisted after gel filtration, and the active form of the enzyme did not contain bound manganese measured by using 54Mn2+. A contaminating p-nitrophenylphosphatase was activated by either Mn2+ (K0.5 of 10(-4) M) or Mg2+ (K0.5 of 10(-3) M). Unlike the protein phosphatase this enzyme was inactive following removal of the metal ions by gel filtration. The phosphatase complex could be dissociated into its component subunits by precipitation with 50% acetone at 20 degrees C in the presence of an inert divalent cation, reducing agent, and bovine serum albumin. Two catalytic subunits were quantitatively recovered; one of Mr 83000 was a trypsin-sensitive manganese-activated phosphatase and the second of Mr 32000 was trypsin-stable and metal ion dependent. Both enzymes were effective in catalyzing the dephosphorylation of either phosphorylase a or the regulatory subunit of adenosine cyclic 3',5'-phosphate (cAMP) dependent protein kinase, but neither subunit possessed p-nitrophenylphosphatase activity.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Brautigan DL,Picton C,Fischer EH

doi

10.1021/bi00566a019

subject

Has Abstract

pub_date

1980-12-09 00:00:00

pages

5787-94

issue

25

eissn

0006-2960

issn

1520-4995

journal_volume

19

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Mobilities of the inner three core residues and the Man(alpha 1--6) branch of the glycan at Asn78 of the alpha-subunit of human chorionic gonadotropin are restricted by the protein.

    abstract：:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone involved in the maintenance of the corpus luteum in early pregnancy. The free alpha-subunit of hCG has a biological activity of its own, namely, stimulation of prolactin secretion from term pregnancy decidual cells [Blithe, D. L., et al. (1991)...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9718548

    authors： van Zuylen CW,de Beer T,Leeflang BR,Boelens R,Kaptein R,Kamerling JP,Vliegenthart JF

    更新日期：1998-02-17 00:00:00

  • Structure and mechanistic implications of a uroporphyrinogen III synthase-product complex.

    abstract：:Uroporphyrinogen III synthase (U3S) catalyzes the asymmetrical cyclization of a linear tetrapyrrole to form the physiologically relevant uroporphyrinogen III (uro'gen III) isomer during heme biosynthesis. Here, we report four apoenzyme and one product complex crystal structures of the Thermus thermophilus (HB27) U3S p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi800635y

    authors： Schubert HL,Phillips JD,Heroux A,Hill CP

    更新日期：2008-08-19 00:00:00

  • Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds as DNA-protein cross-linking agents: potential suicide DNA lesions.

    abstract：:Homodinuclear (Pt,Pt) and heterodinuclear (Ru,Pt) metal compounds having the generalized formula M(a)NH2(CH)4NH2M(b) are shown to form specific DNA lesions which can efficiently cross-link proteins to DNA. In this study, the homodinuclear case is represented by M(a) = M(b) = [cis-Pt(Cl2)-(NH3)] and the heterodinuclear...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00095a007

    authors： Van Houten B,Illenye S,Qu Y,Farrell N

    更新日期：1993-11-09 00:00:00

  • NMR structure of navel orangeworm moth pheromone-binding protein (AtraPBP1): implications for pH-sensitive pheromone detection.

    abstract：:The navel orangeworm, Amyelois transitella (Walker), is an agricultural insect pest that can be controlled by disrupting male-female communication with sex pheromones, a technique known as mating disruption. Insect pheromone-binding proteins (PBPs) provide fast transport of hydrophobic pheromones through the aqueous s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9020132

    authors： Xu X,Xu W,Rayo J,Ishida Y,Leal WS,Ames JB

    更新日期：2010-02-23 00:00:00

  • Molecular characterization of the AP180 coated vesicle assembly protein.

    abstract：:Recently, a new clathrin assembly protein (AP180) has been purified from coated vesicles of bovine brain (Ahle & Ungewickell, 1986). This protein has been shown to promote polymerization of clathrin into a homogeneous population of baskets under conditions where pure clathrin does not polymerize by itself. We have pur...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00416a040

    authors： Prasad K,Lippoldt RE

    更新日期：1988-08-09 00:00:00

  • Identification of the six ligands to manganese(II) in transition-state-analogue complexes of creatine kinase: oxygen-17 superhyperfine coupling from selectively labeled ligands.

    abstract：:The complete coordination scheme for Mn(II) in transition-state-analogue complexes with creatine kinase has been determined by electron paramagnetic resonance (EPR) spectroscopy. Perturbations in the EPR spectra for Mn(II) due to superhyperfine coupling to 17O of selectively labeled ligands have been used to identify ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00565a002

    authors： Reed GH,Leyh TS

    更新日期：1980-11-25 00:00:00

  • Phospholipid-binding properties of bovine factor V and factor Va.

    abstract：:Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00587a023

    authors： Bloom JW,Nesheim ME,Mann KG

    更新日期：1979-10-02 00:00:00

  • Selective photoinduced uncoupling of the response of adenylate cyclase to gonadotropins by 5-iodonaphthyl 1-azide.

    abstract：:5-Iodonaphthyl 1-azide (INA) has been previously shown to selectively label, on photolysis, only those proteins in contact with the membrane lipids. Low concentrations (less than 10 microM) of INA added to rat ovarian plasma membranes induced, on photoactivation, a selective and complete loss of the response of the ad...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00298a016

    authors： Raviv Y,Bercovici T,Gitler C,Salomon Y

    更新日期：1984-01-31 00:00:00

  • Sequence-selective targeting of long stretches of the DNA minor groove by a novel dimeric bis-benzimidazole.

    abstract：:A dimeric bis-benzimidazole molecule has been designed by computer modeling to bind to a DNA sequence via the DNA minor groove that covers a complete turn of B-DNA. A series of bis-benzimidazole dimers incorporating a -O-(CH(2))(n)()-X-CH(2))(n)()-O- linker, with n = 2 or 3 and X = O or N(+)H(Me), were screened for th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi026926w

    authors： Joubert A,Sun XW,Johansson E,Bailly C,Mann J,Neidle S

    更新日期：2003-05-27 00:00:00

  • Proteolytic activity of alpha 2-macroglobulin-enzyme complexes toward human factor VIII/von Willebrand factor.

    abstract：:The low level of enzymatic activity of certain alpha 2-macroglobulin-proteinase complexes could be important to the function of factor VIII/von Willebrand glycoprotein since it is especially sensitive to proteolytic cleavage. To test this possibility, complexes of alpha 2-macroglobulin with plasmin, trypsin, and throm...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00275a018

    authors： Switzer ME,Gordon HJ,McKee PA

    更新日期：1983-03-15 00:00:00

  • Role of Ca(2+) in the Stability and Function of TMEM16F and 16K.

    abstract：:There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+),...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.6b00176

    authors： Ishihara K,Suzuki J,Nagata S

    更新日期：2016-06-14 00:00:00

  • tRNA and the guanosinetriphosphatase activity of elongation factor Tu.

    abstract：:Different sites of the tRNA molecule influence the activity of the elongation factor Tu (EF-Tu) center for GTP hydrolysis [Parlato, G., Pizzano, R., Picone, D., Guesnet, J., Fasano, O., & Parmeggiani, A. (1983) J. Biol. Chem. 258, 995-1000]. Continuing these studies, we have investigated some aspects of (a) the effect...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00427a044

    authors： Swart GW,Parmeggiani A

    更新日期：1989-01-10 00:00:00

  • Comparative Analysis of CPI-Motif Regulation of Biochemical Functions of Actin Capping Protein.

    abstract：:The heterodimeric actin capping protein (CP) is regulated by a set of proteins that contain CP-interacting (CPI) motifs. Outside of the CPI motif, the sequences of these proteins are unrelated and distinct. The CPI motif and surrounding sequences are conserved within a given protein family, when compared to those of o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00092

    authors： McConnell P,Mekel M,Kozlov AG,Mooren OL,Lohman TM,Cooper JA

    更新日期：2020-03-24 00:00:00

  • The amino acid sequence of a carboxypeptidase inhibitor from potatoes.

    abstract：:The carboxypeptidase inhibitor from Russet Burbank potatoes (C. A. Ryan et al. (1974b), J. Biol. Chem 249, 5495) is a mixture of approximately equal amounts of two polypeptide chains containing 38 and 39 amino acid residues, respectively. The chains differ in their amino terminal sequence only, one beginning with smal...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00677a036

    authors： Hass GM,Nau H,Biemann K,Grahn DT,Ericsson LH,Neurath H

    更新日期：1975-03-25 00:00:00

  • Dimorphism in bile salt/lecithin mixed micelles.

    abstract：:We investigated the dependence of certain properties of bile salt/lecithin mixed micelles on the molar ratio of the two components. UV absorption suggested that the micellar system is fundamentally altered on passing through a bile salt:lecithin molar ratio of 1.8:1. Differential scanning calorimetry confirmed this tr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00505a029

    authors： Claffey WJ,Holzbach RT

    更新日期：1981-01-20 00:00:00

  • Recruitment of a double bond isomerase to serve as a reductive dehalogenase during biodegradation of pentachlorophenol.

    abstract：:Tetrachlorohydroquinone dehalogenase catalyzes the replacement of chlorine atoms on tetrachlorohydroquinone and trichlorohydroquinone with hydrogen atoms during the biodegradation of pentachlorophenol by Sphingomonas chlorophenolica. The sequence of the active site region of tetrachlorohydroquinone dehalogenase is ver...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9923813

    authors： Anandarajah K,Kiefer PM Jr,Donohoe BS,Copley SD

    更新日期：2000-05-09 00:00:00

  • Morin inhibits the early stages of amyloid β-peptide aggregation by altering tertiary and quaternary interactions to produce "off-pathway" structures.

    abstract：:Alzheimer's disease is a debilitating neurodegenerative disorder whose pathology has been linked to the aggregation and deposition of the amyloid β-peptide (Aβ) in neural tissue. A truly effective therapeutic agent remains elusive, and attention has recently turned to the use of natural products as effective antiaggre...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi300113x

    authors： Lemkul JA,Bevan DR

    更新日期：2012-07-31 00:00:00

  • Characterization of the metal ion binding helix-hairpin-helix motifs in human DNA polymerase beta by X-ray structural analysis.

    abstract：:X-ray crystallographic studies have shown that DNA binding by human polymerase beta (pol beta) occurs primarily through two structurally and sequentially homologous helix-hairpin-helix (HhH) motifs, one in the fingers subdomain and the other in the 8-kDa domain [Pelletier, H., Sawaya, M. R., Wolfle, W., Wilson, S. H.,...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960790i

    authors： Pelletier H,Sawaya MR

    更新日期：1996-10-01 00:00:00

  • Two promoters in the bovine adrenodoxin gene and the role of associated, unique cAMP-responsive sequences.

    abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00123a027

    authors： Chen JY,Waterman MR

    更新日期：1992-03-03 00:00:00

  • solution structure, backbone dynamics, and interaction with Cdc42 of Salmonella guanine nucleotide exchange factor SopE2.

    abstract：:SopE and SopE2 are delivered by the Salmonella type III secretion system into eukaryotic cells to promote cell invasion. SopE and SopE2 are potent guanine nucleotide exchange factors (GEFs) for Rho GTPases Cdc42 and Rac1 and constitute a novel class of Rho GEFs. Although the sequence of SopE-like GEFs is not at all ho...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0490744

    authors： Williams C,Galyov EE,Bagby S

    更新日期：2004-09-28 00:00:00

  • A network of conserved interactions regulates the allosteric signal in a glutamine amidotransferase.

    abstract：:We have combined equilibrium and steered molecular dynamics (SMD) simulations with principal component and correlation analyses to probe the mechanism of allosteric regulation in imidazole glycerol phosphate (IGP) synthase. An evolutionary analysis of IGP synthase revealed a conserved network of interactions leading f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi061708e

    authors： Amaro RE,Sethi A,Myers RS,Davisson VJ,Luthey-Schulten ZA

    更新日期：2007-02-27 00:00:00

  • Cavitation as a mechanism of substrate discrimination by adenylosuccinate synthetases.

    abstract：:Adenylosuccinate synthetase catalyzes the first committed step in the de novo biosynthesis of AMP, coupling L-aspartate and IMP to form adenylosuccinate. Km values of IMP and 2'-deoxy-IMP are nearly identical with each substrate supporting comparable maximal velocities. Nonetheless, the Km value for L-aspartate and th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0607498

    authors： Iancu CV,Zhou Y,Borza T,Fromm HJ,Honzatko RB

    更新日期：2006-09-26 00:00:00

  • Topographical localization of peroxisomal acyl-CoA ligases: differential localization of palmitoyl-CoA and lignoceroyl-CoA ligases.

    abstract：:We found that peroxisomal lignoceroyl-CoA ligase, like palmitoyl-CoA ligase, is present in the peroxisomal membrane whereas the peroxisomal beta-oxidation enzyme system is localized in the matrix. To further define the role of peroxisomal acyl-CoA ligases (membrane component) in providing acyl-CoA for peroxisomal beta...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00468a027

    authors： Lazo O,Contreras M,Singh I

    更新日期：1990-04-24 00:00:00

  • The NBDY Microprotein Regulates Cellular RNA Decapping.

    abstract：:Proteogenomic identification of translated small open reading frames in humans has revealed thousands of microproteins, or polypeptides of fewer than 100 amino acids, that were previously invisible to geneticists. Hundreds of microproteins have been shown to be essential for cell growth and proliferation, and many reg...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00672

    authors： Na Z,Luo Y,Schofield JA,Smelyansky S,Khitun A,Muthukumar S,Valkov E,Simon MD,Slavoff SA

    更新日期：2020-10-27 00:00:00

  • Interactions between heme d and heme b595 in quinol oxidase bd from Escherichia coli: a photoselection study using femtosecond spectroscopy.

    abstract：:Femtosecond spectroscopy was performed on CO-liganded (fully reduced and mixed-valence states) and O(2)-liganded quinol oxidase bd from Escherichia coli. Substantial polarization effects, unprecedented for optical studies of heme proteins, were observed in the CO photodissociation spectra, implying interactions betwee...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0158019

    authors： Borisov VB,Liebl U,Rappaport F,Martin JL,Zhang J,Gennis RB,Konstantinov AA,Vos MH

    更新日期：2002-02-05 00:00:00

  • Solution structure of BmKalphaIT01, an alpha-insect toxin from the venom of the Chinese scorpion Buthus martensii Karsch.

    abstract：:The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmKalphaIT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmKalphaIT01 has been suggested to be a natural mutant of alpha-insec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7006788

    authors： Tong X,Zhu J,Ma Y,Chen X,Wu G,He F,Cao C,Wu H

    更新日期：2007-10-09 00:00:00

  • Dissecting the catalytic mechanism of betaine-homocysteine S-methyltransferase by use of intrinsic tryptophan fluorescence and site-directed mutagenesis.

    abstract：:Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049821x

    authors： Castro C,Gratson AA,Evans JC,Jiracek J,Collinsová M,Ludwig ML,Garrow TA

    更新日期：2004-05-11 00:00:00

  • Transcriptional regulation of the Xenopus laevis B beta fibrinogen subunit gene by glucocorticoids and hepatocyte nuclear factor 1: analysis by transfection into primary liver cells.

    abstract：:The blood-clotting protein fibrinogen is composed of three subunits, designated A alpha, B beta, and gamma, which are encoded by a family of related genes. As part of the acute-phase response, expression of the fibrinogen genes is coordinately regulated in the liver by glucocorticoids. To understand the factors underl...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00094a020

    authors： Roberts LR,Nichols LA,Holland LJ

    更新日期：1993-11-02 00:00:00

  • (Na, K)ATPase activity in membrane preparations of ouabain-resistant HeLa cells.

    abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00642a600

    authors： Robbins AR,Baker RM

    更新日期：1977-11-15 00:00:00

  • Mycoplasma gallisepticum produces a histone-like protein that recognizes base mismatches in DNA.

    abstract：:Mycoplasmas are the smallest known microorganisms, with drastically reduced genome sizes. One of the essential biochemical pathways lost in mycoplasmas is methylation-mediated DNA repair (MMR), which is responsible for correction of base substitutions, insertions, and deletions in both bacteria and higher organisms. W...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi2009097

    authors： Kamashev D,Oberto J,Serebryakova M,Gorbachev A,Zhukova Y,Levitskii S,Mazur AK,Govorun V

    更新日期：2011-10-11 00:00:00