Abstract：
:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA contains sequences derived from a different exon encoding the mitochondrial leader sequence. To define the sequences necessary for the synthesis of these two types of mRNA and to determine if the synthesis of each is driven by a separate promoter, 5'-regions of the adrenodoxin gene were inserted upstream from two different reporter genes and tested for promoter/enhancer regulatory activity by transient transfection into mouse adrenocortical Y1 tumor cells. The results clearly demonstrated that the bovine adrenodoxin gene contains two functional promoters; one, ADXP1, located in the 5'-flanking region gives rise to the minor form of mRNA, and a second, stronger promoter, ADXP2, which maps within intron 1 gives rise to the major form of mRNA. Unique cAMP-responsive sequences were found upstream from each promoter which share no sequence homology to the consensus CRE (cAMP-responsive element). Upon transient expression, the cAMP-responsive sequence associated with the ADXP2 promoter, termed CRS2, confers the cAMP responsiveness to stimulate the transcription of the linked beta-globin reporter gene regardless of whether the adrenodoxin ADXP2 promoter or the beta-globin promoter was utilized.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Chen JY,Waterman MRdoi
10.1021/bi00123a027subject
Has Abstractpub_date
1992-03-03 00:00:00pages
2400-7issue
8eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:To test whether the Y-shaped conformation of the hammerhead ribozyme is maintained throughout the catalytic pathway, the cleavage properties of circular substrates which bind the ribozyme through helices I and II were determined. Constraining the position of helices I and II in this manner did not significantly alter ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980307z
更新日期：1998-06-30 00:00:00
abstract：:The activation of coagulation factor X by tissue factor (TF) and coagulation factor VIIa (VIIa) on a phospholipid surface is thought to be the key step in the initiation of blood coagulation. In this reaction, the product, fXa, is transiently and reversibly bound to the TF-VIIa enzyme complex. This in effect leads to ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700136a
更新日期：2007-05-22 00:00:00
abstract：:Integral membrane proteins, which include many cellular effector proteins and drug targets, can be difficult to produce, purify, and manipulate. Although the isolated ectodomains of many membrane proteins can be expressed as water soluble proteins, biological activity is frequently lost when these proteins are release...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052600m
更新日期：2006-03-21 00:00:00
abstract：:Apo horse spleen ferritin (apo HoSF) was reconstituted to various core sizes (100-3500 Fe3+/HoSF) by depositing Fe(OH)3 within the hollow HoSF interior by air oxidation of Fe2+. Fe2+ and phosphate (Pi) were then added anaerobically at a 1:4 ratio, and both Fe2+ and Pi were incorporated into the HoSF cores. The resulti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982727u
更新日期：1999-05-18 00:00:00
abstract：:A 150-kDa DNA-dependent ATPase composed of 83/68-kDa subunits was previously reported to cofractionate with a 21S complex of enzymes for DNA synthesis from HeLa cells (Vishwanatha, J. K., & Baril, E. F. (1990) Biochemistry 29, 8753-8759). The DNA-dependent ATPase was purified to electrophoretic homogeneity from a HeLa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00194a021
更新日期：1994-07-19 00:00:00
abstract：:It has been suggested that the mechanism of alkaline phosphatase (AP) is associative, or triester-like, because phosphorothioate monoesters are hydrolyzed by AP approximately 10(2)-fold slower than phosphate monoesters. This "thio effect" is similar to that observed for the nonenzymatic hydrolysis of phosphate trieste...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000899x
更新日期：2000-08-08 00:00:00
abstract：:A soluble Ca2+/calmodulin-dependent protein kinase has been purified from rat brain to near homogeneity by using casein as substrate. The enzyme was purified by using hydroxylapatite adsorption chromatography, phosphocellulose ion-exchange chromatography, Sepharose 6B gel filtration, affinity chromatography using calm...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00318a018
更新日期：1984-11-06 00:00:00
abstract：:The cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA has been studied by electron microscopy. Analysis of the electron microscopic data by means of a simple statistical mechanical model has yielded a value of 3.8-7.6 X 10(10) l./mol for the cooperative binding constant in 0.15 M...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00696a023
更新日期：1975-12-16 00:00:00
abstract：:Multiply branched polylactosaminoglycans are expressed in glycoproteins and glycolipids of many cells. Interest in their biology stems from their abundant expression in early embryonal cells and from their ability to carry multiple lectin-binding determinants, which makes them prominent ligands and antagonists of cell...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9627673
更新日期：1997-06-10 00:00:00
abstract：:By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b00500
更新日期：2017-09-19 00:00:00
abstract：:Intermolecular triplex DNA is stabilized by metal cations and polyamines which reduce repulsion between the negatively charged phosphates of the three nucleic acid strands. We use a quantitative chemical-probing assay involving protection of duplex guanines in a homopyrimidine.homopurine (Py.Pu) sequence from dimethyl...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00045a033
更新日期：1995-11-14 00:00:00
abstract：:Phosphatase of regenerating liver 3 (PRL-3) is suggested as a biomarker and therapeutic target in several cancers. It has a well-established causative role in cancer metastasis. However, little is known about its natural substrates, pathways, and biological functions, and only a few protein substrates have been sugges...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi201095z
更新日期：2011-09-06 00:00:00
abstract：:We investigated the effects of MA-5, a human-specific monoclonal antibody to the insulin receptor alpha-subunit, on transmembrane signaling in cell lines transfected with and expressing both normal human insulin receptors and receptors mutated in their beta-subunit tyrosine kinase domains. In cell lines expressing nor...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00116a025
更新日期：1992-01-14 00:00:00
abstract：:Factor VIII (fVIII) is synthesized as a single chain having a domainal sequence A1-A2-B-A3-C1-C2. Analysis of the proteolyic cleavage of fVIII by thrombin by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) identifies three fragments designated fVIIIA1, fVIIIA2, and fVIIIA3-C1-C2 with fragment(s) d...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00428a038
更新日期：1989-01-24 00:00:00
abstract：:In an effort to better understand protein-protein photoaffinity cross-linking using aryl azides, we have tested a number of factors influencing the cross-linking of the sigma 70 subunit of Escherichia coli RNA polymerase to core RNA polymerase. These factors include the effect of the incubations necessary for the deri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00206a012
更新日期：1994-10-11 00:00:00
abstract：:Binding of the nonhydrolyzable ATP analogue 5'-adenylyl-beta, gamma-imidodiphosphate (ADPNP) to Escherichia coli DNA gyrase can lead to a limited noncatalytic supercoiling of DNA. Here we examine the efficiency of coupling between ADPNP binding and the change in linking number either of positively or negatively superc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952433y
更新日期：1996-02-06 00:00:00
abstract：:The membrane-bound state of the gene 9 minor coat protein of bacteriophage M13 was studied in various membrane-mimicking systems, including organic solvents, detergent micelles, and phospholipid bilayers. For this purpose we determined the conformational and aggregational properties of the chemically synthesized prote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981149e
更新日期：1999-01-19 00:00:00
abstract：:Quinolinate phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) catalyzes the formation of nicotinate mononucleotide, carbon dioxide, and pyrophosphate from 5-phosphoribosyl 1-pyrophosphate (PRPP) and quinolinic acid (QA, pyridine 2,3-dicarboxylic acid). The enzyme is the only type II PRTase whose X-ray structure is kno...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi012148g
更新日期：2002-03-12 00:00:00
abstract：:During T cell signaling, Itk selectively phosphorylates a tyrosine within its own SH3 domain and a tyrosine within PLCgamma1. We find that the remote SH2 domain in each of these substrates is required to achieve efficient tyrosine phosphorylation by Itk and extend this observation to two other Tec family kinases, Btk ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700127c
更新日期：2007-05-08 00:00:00
abstract：:Recently, it has been shown that the activation of the Drosophila EGF receptor (DER) by its natural ligand Spitz is inhibited by Argos [Schweitzer, R., et al. (1995) Nature 376, 699-702]. Argos and Spitz both have an EGF-like domain which in the case of Argos differs from that of Spitz and other EGF receptor agonists ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970227f
更新日期：1997-06-17 00:00:00
abstract：:Diethyl pyrocarbonate (DEP) is an electrophilic reagent that is used to modify reversibly the histidine residues of proteins. Unfortunately, the lability of the acylated histidine adduct usually does not permit the isolation and identification of the modified histidine. By use of 500-MHz proton NMR spectroscopy, it ha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00445a004
更新日期：1989-09-19 00:00:00
abstract：:The stability of three forms of glucoamylase from Aspergillus niger has been investigated by differential scanning and isothermal titration calorimetry: Glucoamylase 1 (GA1), which consists of a catalytic domain and a starch-binding domain (SBD) connected by a heavily O-glycosylated linker region; glucoamylase 2 (GA2)...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990185q
更新日期：1999-05-11 00:00:00
abstract：:Transcripts of Saccharomyces cerevisiae nuclear tRNA genes are normally terminated within a few nucleotides of the tRNA coding region, in contrast to mitochondrially encoded tRNAs, which are contained within polycistronic transcripts and thus require 3'-processing by mitochondrial endonucleases. We show that 3'-proces...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00159a024
更新日期：1992-11-10 00:00:00
abstract：:The head of the P22 bacteriophage is interrupted by a unique dodecameric portal vertex that serves as a conduit for the entrance and exit of the DNA. Here, the in vitro unfolding/refolding processes of the portal protein of P22 were investigated at different temperatures (1, 25, and 37 degrees C) through the use of ur...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700006d
更新日期：2007-06-26 00:00:00
abstract：:The interaction of a nitroxide spin-labeled derivative of ouabain with sheep kidney Na,K-ATPase and the motional behavior of the ouabain spin label-Na,K-ATPase complex have been studied by means of electron paramagnetic resonance (EPR) and saturation-transfer EPR (ST-EPR). Spin-labeled ouabain binds with high affinity...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00122a019
更新日期：1992-02-25 00:00:00
abstract：:We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi963124q
更新日期：1997-06-17 00:00:00
abstract：:Growth factor receptor-bound protein 14 (Grb14) is involved in growth factor receptor tyrosine kinase signaling. Here we report that light causes a major redistribution of Grb14 among the individual subcellular compartments of the retinal rod photoreceptor. Grb14 is localized predominantly to the inner segment, nuclea...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9000062
更新日期：2009-06-23 00:00:00
abstract：:The NuoD segment (homologue of mitochondrial 49 kDa subunit) of the proton-translocating NADH:quinone oxidoreductase (complex I/NDH-1) from Escherichia coli is in the hydrophilic domain and bears many highly conserved amino acid residues. The three-dimensional structural model of NDH-1 suggests that the NuoD segment, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi501403t
更新日期：2015-01-27 00:00:00
abstract：:The NO adducts of leghemoglobin (Lb) are implicated in biological processes, but only the adduct with ferrous Lb (Lb(II)NO) has been characterized previously. We report the first characterization of ferric nitrosylleghemoglobin (Lb(III)NO) and XAS experiments performed on frozen aqueous solutions of Lb(II)NO and Lb(II...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990730n
更新日期：1999-12-14 00:00:00
abstract：:Prothrombin is the precursor of thrombin, a central enzyme in coagulation. Autoantibodies to prothrombin are associated with thromboembolism, but the mechanisms by which the antibodies modulate the coagulation processes are not understood. We screened a panel of 34 monoclonal antibody light chains isolated from patien...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992588w
更新日期：2000-05-30 00:00:00
