当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Stabilization of triple-helical nucleic acids by basic oligopeptides.

Stabilization of triple-helical nucleic acids by basic oligopeptides.

Abstract：

:Intermolecular triplex DNA is stabilized by metal cations and polyamines which reduce repulsion between the negatively charged phosphates of the three nucleic acid strands. We use a quantitative chemical-probing assay involving protection of duplex guanines in a homopyrimidine.homopurine (Py.Pu) sequence from dimethyl sulfate modification to study effects of basic oligopeptides on the stability of triplex DNA. An intermolecular protonated pyrimidine.purine.pyrimidine (Py.Pu*Py) triplex formed readily between a duplex DNA region and a 14-mer pyrimidine triplex-forming oligonucleotide (TFO) at pH 5. The triplex was stabilized at pH by the addition of magnesium ions. In the presence of spermine and lysine-rich peptides, the intermolecular triplex was stabilized up to pH 6.5-7.0. The effective peptide concentration required for stabilization was 10(-5)-10(-2) M. Of the basic peptides studied, pentalysine (Lys-Lys-Lys-Lys-Lys) was the most effective triplex stabilizer. It was effective at concentrations which are lower than those required for Lys-Gly-Lys-Gly-Lys and Lys-Ala-Lys-Ala-Lys and are similar to active concentrations of spermine. Basic peptides were more effective at stabilizing a Py.Pu*Py triplex than a pyrimidine.purine.purine (Py.Pu*Pu) triplex. At 1 mM, Lys-Lys-Lys-Lys-Lys stabilized the Py.Pu*Pu triplex at a level comparable to stabilization by Mn2+ and spermine, whereas Lys-Gly-Lys-Gly-Lys and Lys-Ala-Lys-Ala-Lys resulted in weaker TFO binding. The concentration of TFOs required to form triplex DNA were significantly reduced in the presence of peptides.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Biochemistry

journal_title

Biochemistry

authors

Potaman VN,Sinden RR

doi

10.1021/bi00045a033

subject

Has Abstract

pub_date

1995-11-14 00:00:00

pages

14885-92

issue

45

eissn

0006-2960

issn

1520-4995

journal_volume

34

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Nested allosteric interaction in tarantula hemocyanin revealed through the binding of oxygen and carbon monoxide.

    abstract：:We have examined the competitive binding of oxygen and carbon monoxide to the multisubunit hemocyanin of the tarantula Eurypelma californicum. Employment of high-precision thin-layer methods has enabled detailed characterization of the pure oxygen and pure carbon monoxide binding curves, as well as binding curves perf...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00418a036

    authors： Decker H,Connelly PR,Robert CH,Gill SJ

    更新日期：1988-09-06 00:00:00

  • Nuclear magnetic resonance solution structure of an undecanucleotide duplex with a complementary thymidine base opposite a 10R adduct derived from trans addition of a deoxyadenosine N6-amino group to (-)-(7R,8S,9R,10S)-7,8-dihydroxy- 9,10-epoxy-7,8,9,10-t

    abstract：:The solution structure of a modified undecamer duplex containing (-)-(7R,8S,9R,10S)-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene covalently bonded through trans ring opening at C10 of the epoxide by the N6-amino group of deoxyadenosine (dA) was studied. This diol epoxide 1 diastereomer has the benzylic ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00028a009

    authors： Schurter EJ,Sayer JM,Oh-hara T,Yeh HJ,Yagi H,Luxon BA,Jerina DM,Gorenstein DG

    更新日期：1995-07-18 00:00:00

  • Maintenance of alpha-helical structures by phenyl rings in the active-site tyrosine triad contributes to catalysis and stability of ketosteroid isomerase from Pseudomonas putida biotype B.

    abstract：:Ketosteroid isomerase (KSI) from Pseudomonas putida biotype B is a homodimeric enzyme catalyzing an allylic rearrangement of Delta5-3-ketosteroids at rates comparable with the diffusion-controlled limit. The tyrosine triad (Tyr14.Tyr55.Tyr30) forming a hydrogen-bond network in the apolar active site of KSI has been ch...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015547k

    authors： Nam GH,Jang DS,Cha SS,Lee TH,Kim DH,Hong BH,Yun YS,Oh BH,Choi KY

    更新日期：2001-11-13 00:00:00

  • Acetylation by Eis and Deacetylation by Rv1151c of Mycobacterium tuberculosis HupB: Biochemical and Structural Insight.

    abstract：:Bacterial nucleoid-associated proteins (NAPs) are critical to genome integrity and chromosome maintenance. Post-translational modifications of bacterial NAPs appear to function similarly to their better studied mammalian counterparts. The histone-like NAP HupB from Mycobacterium tuberculosis (Mtb) was previously obser...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b01089

    authors： Green KD,Biswas T,Pang AH,Willby MJ,Reed MS,Stuchlik O,Pohl J,Posey JE,Tsodikov OV,Garneau-Tsodikova S

    更新日期：2018-02-06 00:00:00

  • Dehydroquinate synthase from Escherichia coli: purification, cloning, and construction of overproducers of the enzyme.

    abstract：:Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library. Construction of an appropriate host bearing pLC29-47 results in a strain that produces 20 times more enzyme than ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00314a036

    authors： Frost JW,Bender JL,Kadonaga JT,Knowles JR

    更新日期：1984-09-11 00:00:00

  • Activity of recombinant alpha and beta subunits of casein kinase II from Xenopus laevis.

    abstract：:Casein kinase II (CKII) is a ubiquitous protein kinase, found predominantly in cell nuclei, which has two subunits in a tetrameric alpha 2 beta 2 or alpha alpha' beta 2 conformation. The catalytic center is present in the alpha subunit which is active by itself while beta is a regulatory subunit that can greatly enhan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00079a030

    authors： Hinrichs MV,Jedlicki A,Tellez R,Pongor S,Gatica M,Allende CC,Allende JE

    更新日期：1993-07-20 00:00:00

  • Orientation of the oxygen-evolving manganese complex in a photosystem II membrane preparation: an X-ray absorption spectroscopy study.

    abstract：:X-ray absorption spectroscopy has been performed on oriented photosystem II membrane particles isolated from spinach. Structural features of the tetranuclear Mn cluster and the orientation of the cluster with respect to the lipid bilayer were determined in both the S1 and S2 states of the Kok cycle. Variation of the s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00198a042

    authors： Mukerji I,Andrews JC,DeRose VJ,Latimer MJ,Yachandra VK,Sauer K,Klein MP

    更新日期：1994-08-16 00:00:00

  • Functional tyrosyl residues of carboxypeptidase A. The effect of protein structure on the reactivity of tyrosine-198.

    abstract：:Coupling of bovine carboxypeptidase A with diazotized 5-amino-1H-tetrazole increases esterase activity, decreases peptidase activity slightly, and modifies one tyrosyl residue. Subsequent nitration of the azoenzyme has no further effect on esterase activity, decreases peptidase activity markedly, and modifies a second...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00603a005

    authors： Cueni L,Riordan JF

    更新日期：1978-05-16 00:00:00

  • Photoreversal-dependent release of thymidine and thymidine monophosphate from pyrimidine dimer-containing DNA excision fragments isolated from ultraviolet-damaged human fibroblasts.

    abstract：:To elucidate the enzymatic excision-repair process operative on cyclobutane-type pyrimidine photodimers in human dermal fibroblasts, we have examined excised dimer-containing material recovered in the trichloroacetic acid soluble fraction from far-ultraviolet-irradiated (254 nm, 40 J m-2) and incubated (24 h) cell cul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00357a055

    authors： Weinfeld M,Gentner NE,Johnson LD,Paterson MC

    更新日期：1986-05-06 00:00:00

  • Thermal behavior of synthetic sphingomyelin-cholesterol dispersions.

    abstract：:The thermotropic behavior of aqueous dispersions of palmitoylsphingomyelin-cholesterol and lignoceryl-sphingomyelin-cholesterol mixtures has been examined by high-sensitivity differential scanning calorimetry. When less than 25 mol % cholesterol is mixed with either sphingomyelin, the calorimetric endotherm is compose...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00577a042

    authors： Estep TN,Mountcastle DB,Barenholz Y,Biltonen RL,Thompson TE

    更新日期：1979-05-15 00:00:00

  • Dissecting the catalytic mechanism of betaine-homocysteine S-methyltransferase by use of intrinsic tryptophan fluorescence and site-directed mutagenesis.

    abstract：:Betaine-homocysteine S-methyltransferase (BHMT) is a zinc-dependent enzyme that catalyzes the transfer of a methyl group from glycine betaine (Bet) to homocysteine (Hcy) to form dimethylglycine (DMG) and methionine (Met). Previous studies in other laboratories have indicated that catalysis proceeds through the formati...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi049821x

    authors： Castro C,Gratson AA,Evans JC,Jiracek J,Collinsová M,Ludwig ML,Garrow TA

    更新日期：2004-05-11 00:00:00

  • Picosecond time-resolved fluorescence studies on excitation energy transfer in a histidine 117 mutant of the D2 protein of photosystem II in Synechocystis 6803.

    abstract：:The role of the peripheral reaction center chlorophyll a molecule associated with His117 of the D2 polypeptide in photosystem II was investigated in Synechocystis sp. PCC 6803 using a combination of steady state, pump-probe, and picosecond time-resolved fluorescence spectroscopy. Data were obtained from intact cells a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000476v

    authors： Vasil'ev S,Bruce D

    更新日期：2000-11-21 00:00:00

  • Enzymatic properties of proteolytic derivatives of human alpha-thrombin.

    abstract：:The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00406a049

    authors： Hofsteenge J,Braun PJ,Stone SR

    更新日期：1988-03-22 00:00:00

  • Structure of the human pancreatic cholesterol esterase gene.

    abstract：:The gene for human pancreatic cholesterol esterase consists of 11 exons and 10 introns and is 9.2 kb in length. The last and longest exon (841 nucleotides) is unique to the human gene. Functional amino acids are encoded on separate exons. The leader sequence is encoded by a single exon which carries two additional N-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00141a017

    authors： Kumar BV,Aleman-Gomez JA,Colwell N,Lopez-Candales A,Bosner MS,Spilburg CA,Lowe M,Lange LG

    更新日期：1992-07-07 00:00:00

  • Structure of an RNA hairpin loop with a 5'-CGUUUCG-3' loop motif by heteronuclear NMR spectroscopy and distance geometry.

    abstract：:Structural features of a 19-nucleotide RNA hairpin loop (5'-GGCGUACGUUUCGUACGCC-3'), a loop motif which occurs in eukaryotic 18S rRNA, have been derived using multidimensional heteronuclear NMR spectroscopy in combination with local conformational analysis and torsion angle distance geometry followed by restrained ene...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971207g

    authors： Sich C,Ohlenschläger O,Ramachandran R,Görlach M,Brown LR

    更新日期：1997-11-18 00:00:00

  • N-terminal disordered domain of Saccharomyces cerevisiae Hop1 protein is dispensable for DNA binding, bridging, and synapsis of double-stranded DNA molecules but is necessary for spore formation.

    abstract：:The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi4005528

    authors： Khan K,Madhavan TP,Kshirsagar R,Boosi KN,Sadhale P,Muniyappa K

    更新日期：2013-08-06 00:00:00

  • Role of enzyme-peptide substrate backbone hydrogen bonding in determining protein kinase substrate specificities.

    abstract：:As part of a search for peptides that have specificity for selected protein kinases, the possibility that adenosine cyclic 3',5'-phosphate dependent protein kinase (A-kinase) recognizes the hydrogen-bonding potential of its peptide substrates was investigated. A-Kinase catalyzes the phosphorylation of five N alpha-met...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00388a041

    authors： Thomas NE,Bramson HN,Miller WT,Kaiser ET

    更新日期：1987-07-14 00:00:00

  • Kinetic and equilibrium analysis of the interactions of actomyosin subfragment-1.ADP with beryllium fluoride.

    abstract：:The hypothesis that the stable ternary complex formed between myosin subfragment-1, MgADP and beryllium fluoride (BeF3-), denoted S-1 not equal to .ADP.BeF3-, is an analog of the intermediate state S-1**.ADP.P(i) has been tested in this work by examining the interactions of S-1 not equal to .ADP.BeF3- with actin. Equi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00081a016

    authors： Phan BC,Faller LD,Reisler E

    更新日期：1993-08-03 00:00:00

  • Hemoglobin of the Antarctic fishes Trematomus bernacchii and Trematomus newnesi: structural basis for the increased stability of the liganded tetramer relative to human hemoglobin.

    abstract：:Hemoglobins extracted from fishes that live in temperate waters show little or no dissociation even in the liganded form, unlike human hemoglobin (HbA). To establish whether cold adaptation influences the tendency to dissociate, the dimer-tetramer association constants (L(2,4)) of the carbonmonoxy derivatives of repre...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002297j

    authors： Giangiacomo L,D'Avino R,di Prisco G,Chiancone E

    更新日期：2001-03-13 00:00:00

  • P-Bodies: Composition, Properties, and Functions.

    abstract：:Processing bodies (P-bodies) are cytoplasmic ribonucleoprotein (RNP) granules primarily composed of translationally repressed mRNAs and proteins related to mRNA decay, suggesting roles in post-transcriptional regulation. P-bodies are conserved in eukaryotic cells and exhibit properties of liquid droplets. However, the...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/acs.biochem.7b01162

    authors： Luo Y,Na Z,Slavoff SA

    更新日期：2018-05-01 00:00:00

  • Resonance Raman studies of the flavin and iron-sulfur centers of milk xanthine oxidase.

    abstract：:Resonance Raman spectroscopy has been used to study milk xanthine oxidase, an enzyme containing molybdenum, binuclear iron-sulfur clusters, and FAD as cofactors. The contribution of FAD dominates the resonance Raman spectrum at frequencies above 500 cm-1. As expected, no bands assignable to FAD are observed in deflavo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00332a026

    authors： Willis LJ,Loehr TM

    更新日期：1985-05-21 00:00:00

  • The steric gate amino acid tyrosine 112 is required for efficient mismatched-primer extension by human DNA polymerase kappa.

    abstract：:Human DNA is continuously damaged by exogenous and endogenous genotoxic insults. To counteract DNA damage and ensure the completion of DNA replication, cells possess specialized DNA polymerases (Pols) that bypass a variety of DNA lesions. Human DNA polymerase kappa (hPolkappa) is a member of the Y-family of DNA Pols a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi900153t

    authors： Niimi N,Sassa A,Katafuchi A,Grúz P,Fujimoto H,Bonala RR,Johnson F,Ohta T,Nohmi T

    更新日期：2009-05-26 00:00:00

  • Characterization of the residues involved in the human alpha-thrombin-haemadin complex: an exosite II-binding inhibitor.

    abstract：:Haemadin is a 57-amino acid thrombin inhibitor from the land-living leech Haemadipsa sylvestris, whose structure has recently been determined in complex with human alpha-thrombin. Here we communicate the effect of ionic strength on the kinetics of the inhibition of human alpha-thrombin by haemadin, by using thrombin m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011605q

    authors： Richardson JL,Fuentes-Prior P,Sadler JE,Huber R,Bode W

    更新日期：2002-02-26 00:00:00

  • Stiffness of Cargo-Motor Linkage Tunes Myosin VI Motility and Response to Load.

    abstract：:We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiff...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00422

    authors： Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan S

    更新日期：2019-11-26 00:00:00

  • Probing the mechanism of hamster arylamine N-acetyltransferase 2 acetylation by active site modification, site-directed mutagenesis, and pre-steady state and steady state kinetic studies.

    abstract：:Arylamine N-acetyltransferases (NATs) catalyze an acetyl group transfer from acetyl coenzyme A (AcCoA) to arylamines, hydrazines, and their N-hydroxylated arylamine metabolites. The recently determined three-dimensional structures of prokaryotic NATs have revealed a cysteine protease-like Cys-His-Asp catalytic triad, ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0497244

    authors： Wang H,Vath GM,Gleason KJ,Hanna PE,Wagner CR

    更新日期：2004-06-29 00:00:00

  • Thioltransferase in human red blood cells: purification and properties.

    abstract：:Thioltransferase activity was identified and the enzyme purified to apparent homogeneity from human red blood cells. Activity was measured as glutathione-dependent reduction of the prototype substrate hydroxyethyl disulfide; formation of oxidized glutathione (GSSG) was coupled to NADPH oxidation by GSSG reductase (1 u...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00239a002

    authors： Mieyal JJ,Starke DW,Gravina SA,Dothey C,Chung JS

    更新日期：1991-06-25 00:00:00

  • Perturbation of a tertiary hydrogen bond in barstar by mutagenesis of the sole His residue to Gln leads to accumulation of at least one equilibrium folding intermediate.

    abstract：:A specific tertiary hydrogen bond that is present between the side-chain hydroxyl group of Tyr30 and the side-chain N delta 1 atom of His17 in the small, monomeric, single-domain protein, barstar, has been perturbed by site-directed mutagenesis of the sole histidine residue (His17) to a glutamine residue. The effect o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00005a027

    authors： Nath U,Udgaonkar JB

    更新日期：1995-02-07 00:00:00

  • Conversion of aminodeoxychorismate synthase into anthranilate synthase with Janus mutations: mechanism of pyruvate elimination catalyzed by chorismate enzymes.

    abstract：:The central importance of chorismate enzymes in bacteria, fungi, parasites, and plants combined with their absence in mammals makes them attractive targets for antimicrobials and herbicides. Two of these enzymes, anthranilate synthase (AS) and aminodeoxychorismate synthase (ADCS), are structurally and mechanistically ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b00013

    authors： Culbertson JE,Chung Dh,Ziebart KT,Espiritu E,Toney MD

    更新日期：2015-04-14 00:00:00

  • Heterotropic effectors promote a global conformational change in aspartate transcarbamoylase.

    abstract：:The sigmoidal dependence of activity on substrate concentration exhibited by the regulatory enzyme aspartate transcarbamoylase (ATCase) of Escherichia coli is generally attributed to a ligand-promoted change in the quaternary structure of the enzyme. Although a global conformational change in ATCase upon the binding o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00467a019

    authors： Eisenstein E,Markby DW,Schachman HK

    更新日期：1990-04-17 00:00:00

  • Importance of two buried salt bridges in the stability and folding pathway of barnase.

    abstract：:The importance of two buried salt bridges in barnase in the stability of its folded state, the major transition rate for unfolding, and a folding intermediate has been analyzed by protein engineering, kinetic, and thermodynamic studies. The aspartate residues in the bridges Arg69-Asp93 and Arg83-Asp75 were replaced by...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi952930e

    authors： Tissot AC,Vuilleumier S,Fersht AR

    更新日期：1996-05-28 00:00:00