Abstract:
:We examine the effect of cargo-motor linkage stiffness on the mechanobiological properties of the molecular motor myosin VI. We use the programmability of DNA nanostructures to modulate cargo-motor linkage stiffness and combine it with high-precision optical trapping measurements to measure the effect of linkage stiffness on the motile properties of myosin VI. Our results reveal that a stiff cargo-motor linkage leads to shorter step sizes and load-induced anchoring of myosin VI, while a flexible linkage results in longer steps with frequent detachments from the actin filament under load. Our findings suggest a novel regulatory mechanism for tuning the dual cellular roles of the anchor and transporter ascribed to myosin VI.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Shrivastava R,Rai A,Salapaka M,Sivaramakrishnan Sdoi
10.1021/acs.biochem.9b00422subject
Has Abstractpub_date
2019-11-26 00:00:00pages
4721-4725issue
47eissn
0006-2960issn
1520-4995journal_volume
58pub_type
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