Functional tyrosyl residues of carboxypeptidase A. The effect of protein structure on the reactivity of tyrosine-198.

abstract：:A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, 268, 4197-4206). The procedure consisted of octylglucoside s...

journal_title：Biochemistry

authors： Urbatsch IL,al-Shawi MK,Senior AE

更新日期：1994-06-14 00:00:00

abstract：:To determine the energetic and structural consequences of placing a charged group within the core of a protein, two "buried charge" mutants, Met 102----Lys (M102K) and Leu 133----Asp (L133D) were constructed in phage T4 lysozyme. Both proteins fold at neutral pH, although they are substantially less stable than wild t...

journal_title：Biochemistry

authors： Dao-pin S,Anderson DE,Baase WA,Dahlquist FW,Matthews BW

更新日期：1991-12-10 00:00:00

abstract：:By use of site-directed mutagenesis, each prolyl residue in the lac permease of Escherichia coli at positions 28 (putative helix I), 31 (helix I), 61 (helix II), 89 (helix III), 97 (helix III), 123 (helix IV), 192 (putative hydrophilic region 7), 220 (helix VII), 280 (helix VIII), and 327 [helix X; Lolkema, J. S., et ...

journal_title：Biochemistry

authors： Consler TG,Tsolas O,Kaback HR

更新日期：1991-02-05 00:00:00

abstract：:The interaction between the bacteriophage Q beta coat protein and its specific binding site on Q beta genomic RNA was characterized by using a nitrocellulose filter binding assay. Q beta coat protein bound to a synthetic 29-nucleotide RNA hairpin with an association constant of 400 microM-1 at 4 degrees C, 0.2 M ionic...

journal_title：Biochemistry

authors： Witherell GW,Uhlenbeck OC

更新日期：1989-01-10 00:00:00

abstract：:The catalytically competent active-site structure of a true acylenzyme reaction intermediate of TEM-1 beta-lactamase formed with the kinetically specific spin-labeled substrate 6-N-(2,2,5,5-tetramethyl-1-oxypyrrolinyl-3-carboxyl)-penicillanic acid isolated under cryoenzymologic conditions has been determined by angle-...

journal_title：Biochemistry

authors： Mustafi D,Sosa-Peinado A,Makinen MW

更新日期：2001-02-27 00:00:00

abstract：:Site-directed mutagenesis and high-resolution two-dimensional (2D) proton nuclear magnetic resonance (NMR) were used to probe the structural and functional roles of a highly conserved residue, Asp-49, in the interfacial catalysis by bovine pancreatic phospholipase A2 (PLA2, overexpressed in Escherichia coli). Accordin...

journal_title：Biochemistry

authors： Li Y,Yu BZ,Zhu H,Jain MK,Tsai MD

更新日期：1994-12-13 00:00:00

abstract：:To investigate the effect of a reduced level of selenocysteine (Sec) tRNA[Ser]Sec in selenoprotein biosynthesis, two mouse embryonic stem (ES) cell lines heterozygous for the corresponding gene were generated by homologous recombination of the host genome with targeting vectors encoding a deleted or a disrupted tRNA[S...

journal_title：Biochemistry

authors： Chittum HS,Baek HJ,Diamond AM,Fernandez-Salguero P,Gonzalez F,Ohama T,Hatfield DL,Kuehn M,Lee BJ

更新日期：1997-07-15 00:00:00

abstract：:Protein amyloid fibrils are originally identified as pathological entities in a variety of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. Recent studies have revealed that amyloid fibrils also serve as functional protein assemblies to fulfill a wide range of biological functions. Decip...

journal_title：Biochemistry

authors： Li D,Liu C

更新日期：2020-02-11 00:00:00

abstract：:Electronic absorption, EPR, and resonance Raman spectroscopies revealed that CooA, the CO-sensing transcriptional regulator from Rhodospirillum rubrum, reacts with NO to form a five-coordinate NO-heme. NO must therefore displace both of the heme ligands from six-coordinate, low-spin Fe(II)CooA in forming five-coordina...

journal_title：Biochemistry

authors： Reynolds MF,Parks RB,Burstyn JN,Shelver D,Thorsteinsson MV,Kerby RL,Roberts GP,Vogel KM,Spiro TG

更新日期：2000-01-18 00:00:00

abstract：:Heme oxygenases convert heme to free iron, CO, and biliverdin. Saccharomyces cerevisiae and Candida albicans express putative heme oxygenases that are required for the acquisition of iron from heme, a critical process for fungal survival and virulence. The putative heme oxygenases Hmx1 and CaHmx1 from S. cerevisiae an...

journal_title：Biochemistry

authors： Kim D,Yukl ET,Moënne-Loccoz P,Montellano PR

更新日期：2006-12-12 00:00:00

abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...

journal_title：Biochemistry

authors： Han H,Yang Y,Olesen SH,Becker A,Betzi S,Schönbrunn E

更新日期：2010-05-18 00:00:00

abstract：:The use of derivatives of alpha-thrombin obtained by limited proteolysis, that have only a single peptide bond cleaved, allowed the unequivocal correlation between the change in covalent structure and alteration of the enzymatic properties. beta T-Thrombin contains a single cleavage in the surface loop corresponding t...

journal_title：Biochemistry

authors： Hofsteenge J,Braun PJ,Stone SR

更新日期：1988-03-22 00:00:00

abstract：:Membrane proteins are embedded in a lipid bilayer and maintain strong interactions with lipid molecules. Tightly bound lipids are responsible for vertical positioning and integration of proteins in the membrane and for assembly of multisubunit complexes and occasionally act as substrates. In this work we present the l...

journal_title：Biochemistry

authors： Wöhri AB,Wahlgren WY,Malmerberg E,Johansson LC,Neutze R,Katona G

更新日期：2009-10-20 00:00:00

abstract：:We have investigated the unfolding thermodynamics for incorporating cationic side chains in the Dickerson-Drew dodecamer duplex. Incorporation of two 3-aminopropyl-2'-deoxyuridine residues (one on each self-complementary strand) lowers the stability of the duplex. This reduction is driven by unfavorable heat contribut...

journal_title：Biochemistry

authors： Shikiya R,Li JS,Gold B,Marky LA

更新日期：2005-09-20 00:00:00

abstract：:A bifunctional molecule was genetically engineered which contained an amino-terminal effector domain that bound immunoglobulin Fc (fragment B of staphylococcal protein A) and a carboxyl-terminal domain that bound digoxin [a single-chain Fv (sFv)]. Effector and sFv binding properties were virtually identical with those...

journal_title：Biochemistry

authors： Tai MS,Mudgett-Hunter M,Levinson D,Wu GM,Haber E,Oppermann H,Huston JS

更新日期：1990-09-04 00:00:00

abstract：:Amphotericin B (AmB) is a polyene macrolide antibiotic isolated from Streptomyces nodosus. The antifungal activity of AmB can be attributed to the formation of an ion-channel assembly in the presence of ergosterol (Erg), in which there are two different AmB-Erg orientations, parallel and antiparallel, as reported prev...

journal_title：Biochemistry

authors： Nakagawa Y,Umegawa Y,Matsushita N,Yamamoto T,Tsuchikawa H,Hanashima S,Oishi T,Matsumori N,Murata M

更新日期：2016-06-21 00:00:00

abstract：:Numerous studies have been published stressing the importance of finding ligands that can bind specifically to DNA secondary structures. Several have identified ligands that are presented as having specific binding to the G-quadruplex; however, these were not originally tested on the complementary i-motif structure. T...

journal_title：Biochemistry

authors： Abdelhamid MAS,Gates AJ,Waller ZAE

更新日期：2019-01-29 00:00:00

abstract：:Helix-helix interactions within membranes are dominated by van der Waals packing motifs and side chain-side chain hydrogen bond formation, which act in tandem to determine the residues that comprise the interface between two given helices. To explore in a systematic manner the tertiary contacts between transmembrane h...

journal_title：Biochemistry

authors： Johnson RM,Heslop CL,Deber CM

更新日期：2004-11-16 00:00:00

abstract：:The existence of bacterial K(+)/H(+) antiporters that prevent the overaccumulation of potassium in the cytoplasm was predicted by Peter Mitchell almost 50 years ago. The importance of K(+)/H(+) antiport for bacterial physiology is widely recognized, but its molecular mechanisms remain underinvestigated. Here, we demon...

journal_title：Biochemistry

authors： Resch CT,Winogrodzki JL,Patterson CT,Lind EJ,Quinn MJ,Dibrov P,Häse CC

更新日期：2010-03-23 00:00:00

abstract：:Oligodeoxynucleotides modified site-specifically with dG-(+)-trans- and dG-(+)-cis-anti-BPDE (7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene) or dG-(-)-trans- and dG-(-)-cis-anti-BPDE were used as templates in primer extension reactions catalyzed by the Klenow fragment of Escherichia coli DNA polymerase I....

journal_title：Biochemistry

authors： Shibutani S,Margulis LA,Geacintov NE,Grollman AP

更新日期：1993-07-27 00:00:00

abstract：:HTC cells have been labeled by short exposures to [3H]thymidine in order to identify newly synthesized DNA. By either isolating nuclei directly or isolating them after an extensive fixation with formaldehyde, we have been able to identify two phases in the maturation process of newly replicated chromatin. The first ph...

journal_title：Biochemistry

authors： Smith PA,Jackson V,Chalkley R

更新日期：1984-03-27 00:00:00

abstract：:Dynamic light scattering measurements were carried out on secretin in aqueous solution (2 mM; pH 5.0). The results indicated that the molecule exists as a fairly compact hexamer under these solution conditions. Secondary structural properties of the secretin hexameric complex were evaluated using CD and NMR spectrosco...

journal_title：Biochemistry

authors： Carpenter KA,Schiller PW

更新日期：1998-12-01 00:00:00

abstract：:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 k...

journal_title：Biochemistry

authors： Chryssomallis GS,Torgerson PM,Drickamer HG,Weber G

更新日期：1981-07-07 00:00:00

abstract：:2,3-Butanedione monoxime (BDM) reversibly inhibits force production in muscle. At least part of its action appears to be directly on the contractile apparatus. To understand better its mechanism of action, we studied the effect of BDM on the steps of myosin subfragment 1 Mg(2+)-ATPase in 0.1 M potassium acetate, pH 7....

journal_title：Biochemistry

authors： Herrmann C,Wray J,Travers F,Barman T

更新日期：1992-12-08 00:00:00

abstract：:The DNA interstrand cross-link (ICL) resulting from the C4'-oxidized abasic site (C4-AP) is a unique clustered lesion comprised of a cross-link adjacent to a nick. The ICL is a substrate for the UvrABC nucleotide excision repair system. The strand containing the nick is preferentially incised, but the nick influences ...

journal_title：Biochemistry

authors： Sczepanski JT,Jacobs AC,Van Houten B,Greenberg MM

更新日期：2009-08-18 00:00:00

abstract：:The sucrose (CscB) permease belongs to the oligosaccharide:H(+) symporter family of the Major Facilitator Superfamily and is homologous to the lactose permease from Escherichia coli. Sucrose transport in cells expressing sucrose permease is completely inhibited by N-ethylmaleimide (NEM), suggesting that one or more of...

journal_title：Biochemistry

authors： Sahin-Tóth M,Frillingos S,Lawrence MC,Kaback HR

更新日期：2000-05-23 00:00:00

abstract：:In aspartate aminotransferase, pyridoxal 5'-phosphate (PLP) forms a Schiff base with the epsilon-amino group of Lys258 (internal aldimine). The internal aldimine has a pKa value of 6.8. Binding of a substrate amino acid to the enzyme yields the Michaelis complex, in which PLP still forms the internal aldimine with Lys...

journal_title：Biochemistry

authors： Hayashi H,Mizuguchi H,Kagamiyama H

更新日期：1998-10-27 00:00:00

abstract：:Prokaryotic and eukaryotic cytochrome c oxidases and several bacterial ubiquinol oxidases compose a superfamily of heme-copper oxidases. These enzymes are terminal components of aerobic respiratory chains, the principal energy-generating systems of aerobic organisms. Two such heme-copper oxidases are the aa3-type cyto...

journal_title：Biochemistry

authors： Calhoun MW,Thomas JW,Hill JJ,Hosler JP,Shapleigh JP,Tecklenburg MM,Ferguson-Miller S,Babcock GT,Alben JO,Gennis RB

更新日期：1993-10-12 00:00:00

abstract：:The pivotal role of the Arg-Gly-Asp (RGD) peptide motif in integrin-mediated cell adhesive interactions with extracellular matrix and plasma proteins stimulated the present design of nonpeptidic mimetics of this sequence. To probe the structural requirements for RGD recognition by integrins, we designed various struct...

journal_title：Biochemistry

authors： Greenspoon N,Hershkoviz R,Alon R,Varon D,Shenkman B,Marx G,Federman S,Kapustina G,Lider O

更新日期：1993-02-02 00:00:00

abstract：:Hexose-1-phosphate uridylyltransferase catalyzes the interconversion of UDP-galactose and glucose-1-P with UDP-glucose and galactose-1-P by a double-displacement mechanism through a covalent intermediate (E-UMP), in which UMP is bonded to one of two histidine residues at the active site, H164 or H166. To identify whic...

journal_title：Biochemistry

authors： Kim J,Ruzicka F,Frey PA

更新日期：1990-11-27 00:00:00