P-Bodies: Composition, Properties, and Functions.

abstract：:A fluorescence quenching method was developed for determining partition coefficients and diffusional rates of small molecules in cell membranes. This method involves quenching the fluorescence of carbazole-labeled membranes by hydrophobic molecules that partition into membranes. Cell membrane phospholipids of mouse LM...

journal_title：Biochemistry

authors： Omann GM,Glaser M

更新日期：1984-10-09 00:00:00

abstract：:The solution conformation of the type I collagen alpha-1 chain N-telopeptide has been studied by CD and 1H NMR spectroscopy at 600 MHz in CD3OH/H2O (60/40 v/v) and H2O solutions. The 19 amino acids form the N-terminal end of the alpha-1 polypeptide chain. By the combined application of several two-dimensional, phase-s...

journal_title：Biochemistry

authors： Otter A,Kotovych G,Scott PG

更新日期：1989-10-03 00:00:00

abstract：:Two mutant versions of Escherichia coli aspartate transcarbamylase were created by site-specific mutagenesis. Arg-234 of the 240s loop was replaced by serine in order to help deduce the function of the interactions that normally occur between Arg-234 and both Glu-50 and Gln-231 in the R state of the enzyme. The other ...

journal_title：Biochemistry

authors： Middleton SA,Kantrowitz ER

更新日期：1988-11-15 00:00:00

abstract：:Translocator protein 18 kDa (TSPO) was previously known as the peripheral benzodiazepine receptor (PBR) in eukaryotes, where it is mainly localized to the mitochondrial outer membrane. Considerable evidence indicates that it plays regulatory roles in steroidogenesis and apoptosis and is involved in various human disea...

journal_title：Biochemistry

authors： Li F,Liu J,Liu N,Kuhn LA,Garavito RM,Ferguson-Miller S

更新日期：2016-05-24 00:00:00

abstract：:A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein...

journal_title：Biochemistry

authors： Van Heeke G,Deforce L,Schnizer RA,Shaw R,Couton JM,Shaw G,Song PS,Schuster SM

更新日期：1993-09-28 00:00:00

abstract：:S-Protein/vitronectin is a serum glycoprotein that inhibits the lytic activity of the membrane attack complex of complement, i.e., of the complex including the proteins C5b, C6, C7, C8, and C9n. We show that intact S-protein/vitronectin or its cyanogen bromide generated fragments also inhibit the hemolysis mediated by...

journal_title：Biochemistry

authors： Tschopp J,Masson D,Schäfer S,Peitsch M,Preissner KT

更新日期：1988-05-31 00:00:00

abstract：:The technique of quasi-elastic light scattering was used to measure the translational diffusion coefficient, D, of purified human prothrombin in buffered aqueous solutions and to monitor for the first time the fragmentation of this protein as it is converted to thrombin. The values of D20,w, measured at two different ...

journal_title：Biochemistry

authors： Agarwal GP,Gallagher JG,Aune KC,Armeniades CD

更新日期：1977-05-03 00:00:00

abstract：:Sulfhydryl-reactive derivatives of glucosamine were synthesized as potentially transportable affinity labels of the human erythrocyte hexose carrier. N-Maleoylglycyl derivatives of either 6- or 2-amino-2-deoxy-D-glucopyranose were the most potent inhibitors of 3-O-methylglucose uptake, with concentrations of half-maxi...

journal_title：Biochemistry

authors： May JM

更新日期：1989-02-21 00:00:00

abstract：:The bond cleavage step of pepsinogen activation has been investigated in a kinetic study in which the denatured products of short-term acidifications were separated on SDS-polyacrylamide gels and the peptide products were quantitated by densitometry. Although several peptide products were observed, under the condition...

journal_title：Biochemistry

authors： Glick DM,Shalitin Y,Hilt CR

更新日期：1989-03-21 00:00:00

abstract：:The hydrogen exchange behavior of a four-helix bundle protein in low concentrations of denaturant reveals some partially unfolded forms that are significantly more stable than the fully unfolded state. Kinetic folding of the protein, however, is apparently two-state in the absence of the accumulation of early folding ...

journal_title：Biochemistry

authors： Chu R,Pei W,Takei J,Bai Y

更新日期：2002-06-25 00:00:00

abstract：:Crystallographic analysis revealed that the nicotinamide ring of NAD can bind with multiconformations to aldehyde dehydrogenase (ALDH) (Ni, L., Zhou, J., Hurley, T. D., and Weiner, H. (1999) Protein Sci. 8, 2784-2790). Electron densities can be defined for two conformations, neither of which appears to be compatible w...

journal_title：Biochemistry

authors： Hammen PK,Allali-Hassani A,Hallenga K,Hurley TD,Weiner H

更新日期：2002-06-04 00:00:00

abstract：:We have developed a system for targeting foreign DNA to hepatocytes in vitro using a soluble DNA carrier that takes advantage of receptor-mediated endocytosis to achieve internalization. The idea is based on the fact that hepatocytes possess a unique receptor that binds and internalizes galactose-terminal (asialo)glyc...

journal_title：Biochemistry

authors： Wu GY,Wu CH

更新日期：1988-02-09 00:00:00

abstract：:The bacterial alpha-lytic protease (alphaLP) is synthesized as a precursor containing a large N-terminal pro region (Pro) transiently required for correct folding of the protease [Silen, J. L., and Agard, D. A. (1989) Nature 341, 462-464]. Upon folding, the precursor is autocatalyticly cleaved to yield a tight-binding...

journal_title：Biochemistry

authors： Anderson DE,Peters RJ,Wilk B,Agard DA

更新日期：1999-04-13 00:00:00

abstract：:Reaction of 1-oxiranylpyrene (1-OP) with DNA and the structures of the covalent and noncovalent complexes formed were studied in aqueous media (5 mM phosphate buffer with 0.1 M NaCl, pH 7) by utilizing the techniques of absorption, fluorescence and linear dichroism spectroscopy in order to gain an understanding of pos...

journal_title：Biochemistry

authors： Kim MH,Geacintov NE,Pope M,Harvey RG

更新日期：1984-11-06 00:00:00

abstract：:Comparative primary structural analyses have begun to elucidate polymorphic residues and segments of the class I antigens of the major histocompatibility complex, at least some of which presumably contribute to determinants important in immune recognition events. HLA-A2 structural variants have been described which ar...

journal_title：Biochemistry

authors： Krangel MS,Taketani S,Biddison WE,Strong DM,Strominger JL

更新日期：1982-11-23 00:00:00

abstract：:Complex I, a key component of the mitochondrial respiratory chain, exhibits diminished activity as a result of cardiac ischemia/reperfusion. Cardiac ischemia/reperfusion is associated with increases in the levels of mitochondrial Ca(2+) and pro-oxidants. In the current in vitro study, we sought evidence for a mechanis...

journal_title：Biochemistry

authors： Sadek HA,Szweda PA,Szweda LI

更新日期：2004-07-06 00:00:00

abstract：:Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...

journal_title：Biochemistry

authors： Nguyen AH,Nguyen VT,Kamio Y,Higuchi H

更新日期：2006-02-28 00:00:00

abstract：:Escherichia coli ribonucleotide reductase is an α2β2 complex that catalyzes the conversion of nucleoside 5'-diphosphates (NDPs) to deoxynucleotides (dNDPs). The active site for NDP reduction resides in α2, and the essential diferric-tyrosyl radical (Y(122)(•)) cofactor that initiates transfer of the radical to the act...

journal_title：Biochemistry

authors： Seyedsayamdost MR,Yee CS,Stubbe J

更新日期：2011-03-01 00:00:00

abstract：:The circular dichroism (CD) of Pfl filamentous virus has been examined over the temperature range 0-40 degrees C, in the absence and presence of Hg(II), Ag(I), and Cu(II). Thermal difference CD spectra were obtained by subtraction of spectra recorded above and below a thermally induced structure transition near 12 deg...

journal_title：Biochemistry

authors： Casadevall A,Day LA

更新日期：1988-05-17 00:00:00

abstract：:We determined the magnitude of the bend induced in DNA by an adenine-thymine tract by measuring the rate of cyclization of DNA oligonucleotides containing phased A tracts. A series of linear multimers with 2-bp single-stranded ends, in which the (A.T)6 tracts are separated by CG2-3C sequences and are positioned 10 and...

journal_title：Biochemistry

authors： Koo HS,Drak J,Rice JA,Crothers DM

更新日期：1990-05-01 00:00:00

abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...

journal_title：Biochemistry

authors： Haapalainen AM,Meriläinen G,Pirilä PL,Kondo N,Fukao T,Wierenga RK

更新日期：2007-04-10 00:00:00

abstract：:The polysaccharide-rich cell walls (CWs) of plants perform essential functions such as maintaining tensile strength and allowing plant growth. Using two- and three-dimensional magic-angle-spinning (MAS) solid-state NMR and uniformly (13)C-labeled Arabidopsis thaliana, we have assigned the resonances of the major polys...

journal_title：Biochemistry

authors： Dick-Pérez M,Zhang Y,Hayes J,Salazar A,Zabotina OA,Hong M

更新日期：2011-02-15 00:00:00

abstract：:The human ferritin L-chain cDNA was cloned into a vector for overproduction in Escherichia coli, under the regulation of a lambda promoter. The plasmid obtained contains the full L-chain coding region modified at the first two codons. It is able to direct the synthesis of the L-chain which can constitute up to 15% of ...

journal_title：Biochemistry

authors： Levi S,Salfeld J,Franceschinelli F,Cozzi A,Dorner MH,Arosio P

更新日期：1989-06-13 00:00:00

abstract：:We have analyzed by band-shift assays HeLa cell protein-DNA interactions on a stable cruciform DNA molecule. The stable cruciform was formed by heteroduplexing the HindIII-SphI fragment of SV40 virus DNA that contains the origin of replication with a derivative mutant containing a heterologous substitution at the cent...

journal_title：Biochemistry

authors： Pearson CE,Ruiz MT,Price GB,Zannis-Hadjopoulos M

更新日期：1994-11-29 00:00:00

abstract：:The binding properties of substrates and competitive inhibitors of Escherichia coli cytidine deaminase are compared with those of the fragments obtained by cutting these ligands at several positions including the glycosidic bond. In contrast with the normal substrate cytidine (kcat/Km = 2.6 x 10(6) M-1 s-1), cytosine ...

journal_title：Biochemistry

authors： Carlow D,Wolfenden R

更新日期：1998-08-25 00:00:00

abstract：:A covalent complex between tRNA (m5U54)methyltransferase, 5-fluorouridine tRNA(Phe), and S-adenosyl-L-[methyl-3H]methionine was formed in vitro and purified. Previously, it was shown that in this complex the 6-position of fluorouridine-54 is covalently linked to a catalytic nucleophile and the 5-position is bound to t...

journal_title：Biochemistry

authors： Kealey JT,Santi DV

更新日期：1991-10-08 00:00:00

abstract：:Cholesterol and its hemisuccinate and sulfate derivatives are widely used in studies of purified membrane proteins but are difficult to solubilize in aqueous solution, even in the presence of detergent micelles. Other cholesterol derivatives do not form conventional micelles and lead to viscous solutions. To address t...

journal_title：Biochemistry

authors： Howell SC,Mittal R,Huang L,Travis B,Breyer RM,Sanders CR

更新日期：2010-11-09 00:00:00

abstract：:The luminescence properties (fluorescence and phosphorescence) of luliberin have been investigated at liquid-nitrogen temperature (77 K) in 50% ethylene glycolaqueous buffer at various pH's. Calculation of the energy-transfer efficiency between Trp and Tyr residues leads to an evaluation of the distance separating the...

journal_title：Biochemistry

authors： Marche P,Montenay-Garestier T,Fromageot P,Hélène C

更新日期：1976-12-28 00:00:00

abstract：:Creatininase catalyzes the conversion of creatinine (a biosensor for kidney function) to creatine via a two-step mechanism: water addition followed by ring opening. Water addition is common to other known cyclic amidohydrolases, but the precise mechanism for ring opening is still under debate. The proton donor in this...

journal_title：Biochemistry

authors： Jitonnom J,Mujika JI,van der Kamp MW,Mulholland AJ

更新日期：2017-12-05 00:00:00

abstract：:The dependence of the activity of recombinant activated human protein C (r-APC) on each of its nine gamma-carboxyglutamic (Gla) residues (sequence positions 6, 7, 14, 16, 19, 20, 25, 26, and 29) has been assessed in purified systems and in plasma using r-mutants in which each Gla residue of r-APC was individually alte...

journal_title：Biochemistry

authors： Jhingan A,Zhang L,Christiansen WT,Castellino FJ

更新日期：1994-02-22 00:00:00