Identification of the catalytic nucleophile of tRNA (m5U54)methyltransferase.

abstract：:The helical regions of RNA are generally very stable, but the single-stranded and loop regions often exist as an ensemble of conformations in solution. The theophylline-binding RNA aptamer forms a very stable structure when bound to the bronchodilator theophylline, but the theophylline binding site is not stably forme...

journal_title：Biochemistry

authors： Jucker FM,Phillips RM,McCallum SA,Pardi A

更新日期：2003-03-11 00:00:00

abstract：:The vast majority of characterized oxygenases use bound cofactors to activate molecular oxygen to carry out oxidation chemistry. Here, we show that an enzyme of unknown activity, RhCC from Rhodococcus jostii RHA1, functions as an oxygenase, using 4-hydroxyphenylenolpyruvate as a substrate. This unique and complex reac...

journal_title：Biochemistry

authors： Baas BJ,Poddar H,Geertsema EM,Rozeboom HJ,de Vries MP,Permentier HP,Thunnissen AM,Poelarends GJ

更新日期：2015-02-10 00:00:00

abstract：:Classical strategies for structure analysis of proteins interacting with a lipid phase typically correlate ensemble secondary structure content measurements with changes in the spectroscopic responses of localized aromatic residues or reporter molecules to map regional solvent environments. Deep-UV resonance Raman (DU...

journal_title：Biochemistry

authors： Halsey CM,Oshokoya OO,Jiji RD,Cooley JW

更新日期：2011-08-02 00:00:00

abstract：:Structural properties of heparan sulfate preparations from hog mucosa and beef lung sources were obtained by application of Smith degradation and nitrous acid reactions. Products formed by these reactions indicated that most of the iduronic acid present in these mucopolysaccharides is ester sulfated, whereas N-sulfate...

journal_title：Biochemistry

authors： Cifonelli JA,King JA

更新日期：1977-05-17 00:00:00

abstract：:Aflatoxin B1 (AFB1) exo-8,9-epoxide, the reactive product of the hepatocarcinogen AFB1, is stable in aprotic solvents but hydrolyzes rapidly in H2O at 25 degrees C and pH 7 (t1/2 = 1 s). However, it is also known that some glutathione (GSH) transferases can conjugate the epoxide with GSH to give the adduct in high yie...

journal_title：Biochemistry

authors： Johnson WW,Ueng YF,Widersten M,Mannervik B,Hayes JD,Sherratt PJ,Ketterer B,Guengerich FP

更新日期：1997-03-18 00:00:00

abstract：:Vitronectin is a multifunctional plasma glycoprotein which may regulate the systems related to protease cascades such as the coagulation, fibrinolysis, and complement systems as well as cell adhesion. Solid-phase assays and affinity chromatography on immobilized glycolipids indicated that vitronectin purified under de...

journal_title：Biochemistry

authors： Yoneda A,Ogawa H,Kojima K,Matsumoto I

更新日期：1998-05-05 00:00:00

abstract：:A crude in vitro system which initiates chloroplast DNA synthesis near the D-loop site mapped by electron microscopy [Wu, M., Lou, J. K., Chang, D. Y., Chang, C. H., & Nie, Z. Q. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 6761-6765] consists of soluble proteins and proteins extracted from purified thylakoid membrane. In...

journal_title：Biochemistry

authors： Wang ZF,Yang J,Nie ZQ,Wu M

更新日期：1991-01-29 00:00:00

abstract：:Recent work has resulted in the development of potent inhibitors of oligosaccharyl transferase (OT), the enzyme that catalyzes the cotranslational glycosylation of asparagine [Hendrickson, T. L., Spencer, J. R., Kato, M., and Imperiali, B. (1996) J. Am. Chem. Soc. 118, 7636-7637; Kellenberger, C., Hendrickson, T. L., ...

journal_title：Biochemistry

authors： Eason PD,Imperiali B

更新日期：1999-04-27 00:00:00

abstract：:A total of fifty single site surface phenylalanine substitution mutants have been made in the model protein staphylococcal nuclease. The fifty residues that were replaced with phenylalanine were chosen to give a broad sampling of solvent accessibility, secondary structure, and backbone conformations. The change in the...

journal_title：Biochemistry

authors： Schwehm JM,Kristyanne ES,Biggers CC,Stites WE

更新日期：1998-05-12 00:00:00

abstract：:The importance of two buried salt bridges in barnase in the stability of its folded state, the major transition rate for unfolding, and a folding intermediate has been analyzed by protein engineering, kinetic, and thermodynamic studies. The aspartate residues in the bridges Arg69-Asp93 and Arg83-Asp75 were replaced by...

journal_title：Biochemistry

authors： Tissot AC,Vuilleumier S,Fersht AR

更新日期：1996-05-28 00:00:00

abstract：:In a large number of plant species hydroxynitrile lyases catalyze the decomposition of cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen cyanide serves as a deterrent against herbivores and fungi. In vitro hydroxynitrile lyases are proficient biocatalysts for the stereospecific synthesis ...

journal_title：Biochemistry

authors： Dreveny I,Andryushkova AS,Glieder A,Gruber K,Kratky C

更新日期：2009-04-21 00:00:00

abstract：:The self-association reaction in which four melittin molecules associate to form an aqueously soluble tetramer was studied by fluorescent spectroscopy. At 23 degrees C, pH 7.15, gamma/2 0.50, the dissociation constant, Kd, is 3.20 x 10(-16) M3. At 23 degrees C, gamma/2 0.60, melittin has an amino acyl group with a pro...

journal_title：Biochemistry

authors： Quay SC,Condie CC

更新日期：1983-02-01 00:00:00

abstract：:Thromboxane A(2) receptor (TP receptor), a prostanoid receptor, belongs to the G protein-coupled receptor family, composed of three intracellular loops and three extracellular loops connecting seven transmembrane helices. The highly conserved extracellular domains of the prostanoid receptors were found in the second e...

journal_title：Biochemistry

authors： Ruan KH,So SP,Wu J,Li D,Huang A,Kung J

更新日期：2001-01-09 00:00:00

abstract：:All retroviruses package two copies of their genomes during virus assembly, both of which are required for strand transfer-mediated recombination during reverse transcription. Genome packaging is mediated by interactions between the nucleocapsid (NC) domains of assembling Gag polyproteins and an RNA packaging signal, ...

journal_title：Biochemistry

authors： Dey A,York D,Smalls-Mantey A,Summers MF

更新日期：2005-03-15 00:00:00

abstract：:The human antioxidant protein, HAH1, is an important participant in a Cu(I) transport chain, delivering one Cu(I) ion to the Wilson's (WND) or Menkes disease protein (MNK). Full geometry optimizations and second-derivative calculations were performed on several binding site models using the B3LYP functional to derive ...

journal_title：Biochemistry

authors： Op't Holt BT,Merz KM Jr

更新日期：2007-07-31 00:00:00

abstract：:Pleckstrin homology (PH) domains play a central role in a wide array of signaling pathways by binding second messenger lipids of the phosphatidylinositol phosphate (PIP) lipid family. A given type of PIP lipid is formed in a specific cellular membrane where it is generally a minor component of the bulk lipid mixture. ...

journal_title：Biochemistry

authors： Corbin JA,Dirkx RA,Falke JJ

更新日期：2004-12-28 00:00:00

abstract：:The side-chain dynamics of methyl groups in two structurally related proteins from the fibronectin type III (fnIII) superfamily, the third fnIII domain from human tenascin (TNfn3) and the tenth fnIII domain from human fibronectin (FNfn10), have been studied by NMR spectroscopy. Side-chain order parameters reveal that ...

journal_title：Biochemistry

authors： Best RB,Rutherford TJ,Freund SM,Clarke J

更新日期：2004-02-10 00:00:00

abstract：:Electron paramagnetic resonance studies have indicated that nitrosodisulfonate binds to pig heart citrate synthase. Titration of the enzyme with nitrosodisulfonate revealed several binding sites for the probe per subunit with one site (KD approximately 0.1 mM) having a greater affinity than the others. The substrate, ...

journal_title：Biochemistry

authors： Weidman SW,Drysdale GR

更新日期：1979-08-21 00:00:00

abstract：:Increasing efforts are being invested in the construction of nanostructures with desired shapes and physical and chemical properties. Our strategy involves nanostructure design using naturally occurring protein building blocks. Inspection of the protein structural database (PDB) reveals the richness of the conformatio...

journal_title：Biochemistry

authors： Zheng J,Zanuy D,Haspel N,Tsai CJ,Alemán C,Nussinov R

更新日期：2007-02-06 00:00:00

abstract：:Absorbance difference spectra were recorded at 20 degrees C from 30 ns to milliseconds after photolysis of lauryl maltoside suspensions of artificial visual pigments derived from 9-cis isomers of 5-ethylretinal, 8,16-methanoretinal (a 6-s-trans-bicyclic analogue), or 5-demethyl-8-methylretinal. In all three pigments, ...

journal_title：Biochemistry

authors： Szundi I,de Lera AR,Pazos Y,Alvarez R,Oliana M,Sheves M,Lewis JW,Kliger DS

更新日期：2002-02-12 00:00:00

abstract：:The marked difference between the intra- and extracellular 31P NMR chemical shifts of various phosphoryl compounds when added to a red cell suspension may be largely understood in terms of the effects of hemoglobin on the 31P NMR chemical shifts. The presence of [oxy- or (carbonmonoxy)-] hemoglobin inside the red cell...

journal_title：Biochemistry

authors： Kirk K,Kuchel PW

更新日期：1988-11-29 00:00:00

abstract：:To describe the hydrophobic adsorption of charged molecules to bilayer membranes, one must recognize that the adsorption produces a change in the electrostatic potential at the surface of the membrane. The surface potential produced by the adsorption of the charged molecules can be described most simply by the Gouy eq...

journal_title：Biochemistry

authors： McLaughlin S,Harary H

更新日期：1976-05-04 00:00:00

abstract：:The heme binding sites of rabbit histidine-rich glycoprotein (HRG), 94 kDa, were studied with rose bengal (RB), a fluorescein derivative that associates with histidine residues. Difference absorbance spectra indicate that HRG binds RB at two thermodynamically preferred sites (Kd approximately 2 microM) that are spectr...

journal_title：Biochemistry

authors： Burch MK,Morgan WT

更新日期：1985-10-08 00:00:00

abstract：:A chromatin acceptor protein for the avian oviduct progesterone receptor (PR), termed receptor binding factor 1 (RBF-1), has recently been shown to (1) be a component of the nuclear binding sites (acceptor sites) for PR and (2) generate high-affinity binding sites (termed the RBF-1 class of sites) on avian genomic DNA...

journal_title：Biochemistry

authors： Schuchard M,Subramaniam M,Ruesink T,Spelsberg TC

更新日期：1991-10-01 00:00:00

abstract：:Members of Mononegavirales, the order that includes nonsegmented negative sense RNA viruses (NNSVs), encode a small number of multifunctional proteins. In members of the Filoviridae family, virus protein 35 (VP35) facilitates immune evasion and functions as an obligatory cofactor for viral RNA synthesis. VP35 function...

journal_title：Biochemistry

authors： Chanthamontri CK,Jordan DS,Wang W,Wu C,Lin Y,Brett TJ,Gross ML,Leung DW

更新日期：2019-02-12 00:00:00

abstract：:It has been inferred from structural and computational studies that the mechanism of DNA polymerases involves subtle but important discrete steps that occur between binding and recognition of the correct dNTP and chemical catalysis. These steps potentially include local conformational changes involving active site res...

journal_title：Biochemistry

authors： Balbo PB,Wang EC,Tsai MD

更新日期：2011-11-15 00:00:00

abstract：:The effect of hydrostatic pressure upon solutions of chymotrypsinogen and lysozyme at room temperature has been followed by employing a new technique [Chryssomallis, G. S., Drickamer, H. G., & Weber, G. (1978) J. Appl. Phys. 49, 3084] that permits the measurement of fluorescence polarization at pressures of up to 10 k...

journal_title：Biochemistry

authors： Chryssomallis GS,Torgerson PM,Drickamer HG,Weber G

更新日期：1981-07-07 00:00:00

abstract：:Two forms of cytochrome P-450, designated P-450NMa and P-450NMb, were purified to electrophoretic homogeneity from rabbit nasal microsomes. The purified cytochromes, which contained 14-16 nmol of P-450/mg of protein, exhibited apparent monomeric molecular weights of 49,500 and 51,000, respectively. As indicated by sev...

journal_title：Biochemistry

authors： Ding XX,Coon MJ

更新日期：1988-11-01 00:00:00

abstract：:The extent of rapid (picosecond) backbone motions within the glucocorticoid receptor DNA-binding domain (GR DBD) has been investigated using proton-detected heteronuclear NMR spectroscopy on uniformly 15N-labeled protein fragments containing the GR DBD. Sequence-specific 15N resonance assignments, based on two- and th...

journal_title：Biochemistry

authors： Berglund H,Kovács H,Dahlman-Wright K,Gustafsson JA,Härd T

更新日期：1992-12-08 00:00:00

abstract：:The field of chemical site-selective modification of proteins has progressed extensively in recent decades to enable protein functionalization for imaging, drug delivery, and functional studies. In this Perspective, we provide detailed insight into an alternative use of site-selective protein chemistry to probe the ro...

journal_title：Biochemistry

authors： Bertoldo JB,Terenzi H,Hüttelmaier S,Bernardes GJL

更新日期：2018-10-30 00:00:00