Abstract:
:A synthetic gene coding for the inhibitor protein of bovine heart mitochondrial F1 adenosine triphosphatase was designed and cloned in Escherichia coli. Recombinant F1-ATPase inhibitor protein was overproduced in E. coli and secreted to the periplasmic space. Biologically active recombinant F1-ATPase inhibitor protein was recovered from the bacterial cells by osmotic shock and was purified to near homogeneity in a single cation-exchange chromatography step. The recombinant inhibitor protein was shown to inhibit bovine mitochondrial F1-ATPase in a pH-dependent manner, as well as Saccharomyces cerevisiae mitochondrial F1-ATPase. Thorough analysis of the amino acid sequence revealed a potential coiled-coil structure for the C-terminal portion of the protein. Experimental evidence obtained by circular dichroism analyses supports this prediction and suggests F1I to be a highly stable, mainly alpha-helical protein which displays C-terminal alpha-helical coiled-coil intermolecular interaction.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Van Heeke G,Deforce L,Schnizer RA,Shaw R,Couton JM,Shaw G,Song PS,Schuster SMdoi
10.1021/bi00089a033subject
Has Abstractpub_date
1993-09-28 00:00:00pages
10140-9issue
38eissn
0006-2960issn
1520-4995journal_volume
32pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Helicobacter pylori produces one monofunctional catalase, encoded by katA (hp0875). The crystal structure of H. pylori catalase (HPC) has been determined and refined at 1.6 A with crystallographic agreement factors R and R(free) of 17.4 and 21.9%, respectively. The crystal exhibits P2(1)2(1)2 space group symmetry and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035663i
更新日期:2004-03-23 00:00:00
abstract::Expansions of (CTG)·(CAG) repeated DNAs are the mutagenic cause of 14 neurological diseases, likely arising through the formation and processing of slipped-strand DNAs. These transient intermediates of repeat length mutations are formed by out-of-register mispairing of repeat units on complementary strands. The three-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301369b
更新日期:2013-02-05 00:00:00
abstract::A cyclic nucleotide phosphodiesterase was extensively purified from the 100000g supernatant fraction of human platelets. The purification was 2500-3000-fold with 30% recovery of activity. The enzyme was isolated by DEAE-cellulose chromatography followed by adsorption to blue dextran-Sepharose and elution with cAMP. Th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00303a034
更新日期:1984-04-10 00:00:00
abstract::Histone deacetylase 8 (HDAC8) is a well-characterized member of the class I acetyl-lysine deacetylase (HDAC) family. Previous work has shown that the efficiency of HDAC8-catalyzed deacetylation of a methylcoumarin peptide varies depending on the identity of the divalent metal ion in the HDAC8 active site. Here we demo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00851
更新日期:2017-10-24 00:00:00
abstract::We report nuclear magnetic resonance (NMR) data indicating that the Rieske protein from the cytochrome bc complex of Thermus thermophilus (TtRp) undergoes modest redox-state-dependent and ligand-dependent conformational changes. To test models concerning the mechanism by which TtRp transfers between different sites on...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi400296c
更新日期:2013-04-30 00:00:00
abstract::Purple membranes (PM) from Halobacterium halobium were incorporated into 7.5% polyacrylamide gels to prevent aggregation which occurs in suspensions at low pH. At pH 7.0, the circular dichroism (CD) spectra and visible absorption spectra of light- and dark-adapted bacteriorhodopsin (bR558, respectively) and the flash ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00586a007
更新日期:1979-09-18 00:00:00
abstract::The structure of an analogue of the yeast tRNAPhe T Psi C stem-loop has been determined by NMR spectroscopy and restrained molecular dynamics. The molecule contained the highly conserved modification ribothymidine at its naturally occurring position. The ribothymidine-modified T Psi C stem-loop is the product of the m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990118w
更新日期:1999-07-06 00:00:00
abstract::31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orienta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00507a013
更新日期:1981-02-17 00:00:00
abstract::The chicken and goose egg white lysozymes (ChEWL and GoEWL) are homologues, but differ in substrate specificity. ChEWL catalyzes the hydrolysis of the glycosidic bonds of bacterial peptidoglycans and chitin-derived substrates, while GoEWL is specific for bacterial peptidoglycans. The active-site aspartate 52 residue o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951671q
更新日期:1996-02-13 00:00:00
abstract::The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00394a030
更新日期:1987-10-06 00:00:00
abstract::A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00626a042
更新日期:1977-04-05 00:00:00
abstract::The interactions of a series of spin-labeled fatty acids, in which the nitroxide ring is incorporated in different ways as an integral part of the hydrocarbon chain, with the (Na+,K+)-ATPase in membranes from Squalus acanthias, have been studied by electron spin resonance spectroscopy. The fatty acids are 2,4-, 2,5-, ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00411a004
更新日期:1988-05-31 00:00:00
abstract::The dissociation of the trypsin-sensitive basic tails of the core histones in core chromatin has been followed as a function of [NaCl] using proton NMR spectroscopy. The tails dissociate in a highly cooperative all or none manner over the salt concentration range 0.2-0.6 M, that is, below the salt concentration requir...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00318a037
更新日期:1984-11-06 00:00:00
abstract::Kynureninase is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the hydrolytic cleavage of l-kynurenine to give l-alanine and anthranilic acid. beta-Benzoyl-l-alanine, the analogue of l-kynurenine lacking the aromatic amino group, was shown to a good substrate for kynureninase from Pseudomonas fluorescens, an...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100955b
更新日期:2010-09-14 00:00:00
abstract::Comparisons of catalytic mechanisms have not previously been performed for homologous enzymes from hyperthermophilic and mesophilic sources. Here, the beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to have biophyscial and biochemical pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9814944
更新日期:1998-12-08 00:00:00
abstract::As discovered by Grazi & Trombetta [Grazi, E., & Trombetta, G. (1978) Biochem. J. 175, 361], fructose-1,6-bisphosphate aldolase of rabbit muscle causes the slow formation of inorganic phosphate (Pi) and methylglyoxal when incubated with dihydroxyacetone phosphate (DHAP). In addition, these authors found an acid-labile...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00508a026
更新日期:1981-03-03 00:00:00
abstract::Four new in vivo metabolites of vitamin D3 were isolated from the blood plasma of chicks given large doses of vitamin D3. The metabolites were isolated by methanol-chloroform extraction and a series of chromatographic procedures. By use of mass spectrometry, ultraviolet absorption spectrophotometry, and specific chemi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00529a010
更新日期:1981-12-22 00:00:00
abstract::Purine riboswitches are RNA regulatory elements that control purine metabolism in response to intracellular concentrations of the purine ligands. Conformational changes of the guanine riboswitch aptamer domain induced by guanine binding lead to transcriptional regulation of genes involved in guanine biosynthesis. The ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9019912
更新日期:2010-03-02 00:00:00
abstract::Heparin lyases are valuable tools for generating oligosaccharide fragments and in sequence determination of heparan sulfate (HS). Heparin lyase III is known to cleave the linkages between N-acetylglucosamine (GlcNAc) or N-sulfated glucosamine (GlcNS) and glucuronic acid (GlcA) as the primary sites and the linkages bet...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi036250k
更新日期:2004-07-06 00:00:00
abstract::The protective effect of cyclic stable nitroxide free radicals, having SOD-like activity, against oxidative damage was studied by using Escherichia coli xthA DNA repair-deficient mutant hypersensitive to H2O2. Oxidative damage induced by H2O2 was assayed by monitoring cell survival. The metal chelator 1,10-phenanthrol...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00216a033
更新日期:1991-01-15 00:00:00
abstract::The unfolding and dissociation of the tetrameric enzyme fructose-1,6-bisphosphatase from pig kidney by guanidine hydrochloride have been investigated at equilibrium by monitoring enzyme activity, ANS binding, intrinsic (tyrosine) protein fluorescence, exposure of thiol groups, fluorescence of extrinsic probes (AEDANS,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034203m
更新日期:2003-06-17 00:00:00
abstract::A newly recognized type of protein-ligand interaction phenomenon has resulted in the preparation of simple, nonionic, and highly specific gel derivatives for selective adsorption chromatography. The essential structure of the immobilized ligand can be represented as agarose-CH2CH2SO2CH2CH2SCH2CH2OH, which was prepared...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00396a049
更新日期:1987-11-03 00:00:00
abstract::VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0477034
更新日期:2005-04-26 00:00:00
abstract::Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of no...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060969l
更新日期:2007-03-20 00:00:00
abstract::Homoisocitrate dehydrogenase (HIcDH, 3-carboxy-2-hydroxyadipate dehydrogenase) catalyzes the fourth reaction of the alpha-aminoadipate pathway for lysine biosynthesis, the conversion of homoisocitrate to alpha-ketoadipate using NAD as an oxidizing agent. A chemical mechanism for HIcDH is proposed on the basis of the p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi702361j
更新日期:2008-04-01 00:00:00
abstract::The unique occurrence of G-quadruplexes in the AT-rich genome of human malaria parasite Plasmodium falciparum provides hints about their critical roles in parasite survival, pathogenesis, and host immune evasion. An intriguing question is whether these noncanonical structures can serve as molecular targets for small m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00964
更新日期:2017-12-26 00:00:00
abstract::First identified on the surface of lymphoids as a type II transmembrane protein, CD38 has now been established to have dual functions not only as a receptor but also as a multifunctional enzyme,catalyzing the synthesis of and hydrolysis of a general calcium messenger molecule, cyclic ADP-ribose(cADPR). The receptorial...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:2008-12-30 00:00:00
abstract::The helical regions of RNA are generally very stable, but the single-stranded and loop regions often exist as an ensemble of conformations in solution. The theophylline-binding RNA aptamer forms a very stable structure when bound to the bronchodilator theophylline, but the theophylline binding site is not stably forme...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027103+
更新日期:2003-03-11 00:00:00
abstract::The role of reversible phosphorylation in histamine-induced Ca2+ oscillations in HeLa cells has been investigated by using various activators and inhibitors of protein kinases and phosphatases. Electroporation was employed to introduce impermeable materials into single cells, which proved to be a useful and convenient...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952471h
更新日期:1996-06-04 00:00:00
abstract::All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi047732e
更新日期:2005-04-12 00:00:00