A distinctive RNA fold: the solution structure of an analogue of the yeast tRNAPhe T Psi C domain.

abstract：:D-Xylose isomerase (XI) and triosephosphate isomerase (TIM) catalyze the aldose-ketose isomerization reactions of D-xylose and d-glyceraldehyde 3-phosphate (DGAP), respectively. D-Glyceraldehyde (DGA) is the triose fragment common to the substrates for XI and TIM. The XI-catalyzed isomerization of DGA to give dihydrox...

journal_title：Biochemistry

authors： Toteva MM,Silvaggi NR,Allen KN,Richard JP

更新日期：2011-11-22 00:00:00

abstract：:Photolyases (PHRs) repair the UV-induced photoproducts, cyclobutane pyrimidine dimer (CPD) or pyrimidine-pyrimidone (6-4) photoproduct [(6-4) PP], restoring normal bases to maintain genetic integrity. CPD and (6-4) PP are repaired by substrate-specific PHRs, CPD PHR and (6-4) PHR, respectively. Flavin adenine dinucleo...

journal_title：Biochemistry

authors： Yamada D,Yamamoto J,Zhang Y,Iwata T,Hitomi K,Getzoff ED,Iwai S,Kandori H

更新日期：2016-02-02 00:00:00

abstract：:The post-polyketide synthase (PKS) biosynthetic tailoring of macrolide antibiotics usually involves one or more oxidation reactions catalyzed by cytochrome P450 monooxygenases. As the specificities of members from this class of enzymes vary significantly among PKS gene clusters, the identification and study of new mac...

journal_title：Biochemistry

authors： Betlach MC,Kealey JT,Ashley GW,McDaniel R

更新日期：1998-10-20 00:00:00

abstract：:Alanine scanning mutagenesis, double mutant cycles, and X-ray crystallography were used to characterize the interface between the anti-hen egg white lysozyme (HEL) antibody D1.3 and HEL. Twelve out of the 13 nonglycine contact residues on HEL, as determined by the high-resolution crystal structure of the D1.3-HEL comp...

journal_title：Biochemistry

authors： Dall'Acqua W,Goldman ER,Lin W,Teng C,Tsuchiya D,Li H,Ysern X,Braden BC,Li Y,Smith-Gill SJ,Mariuzza RA

更新日期：1998-06-02 00:00:00

abstract：:The actin cytoskeleton carries out cellular functions, including division, migration, adhesion, and intracellular transport, that require a variety of actin binding proteins, including myosins. Our focus here is on class II nonmuscle myosin isoforms, NMIIA, NMIIB, and NMIIC, and their regulation by the actin binding p...

journal_title：Biochemistry

authors： Barua B,Nagy A,Sellers JR,Hitchcock-DeGregori SE

更新日期：2014-06-24 00:00:00

abstract：:Molecular dynamics (MD) simulations of Thermus thermophilus chorismate mutase substrate complex (TtCM x S) have been carried out at 298 K, 333 K, and the temperature of optimum activity: 343 K. The enzyme exists as trimeric subunits with active sites shared between two neighboring subunits. Two features distinguish in...

journal_title：Biochemistry

authors： Zhang X,Bruice TC

更新日期：2006-07-18 00:00:00

abstract：:We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...

journal_title：Biochemistry

authors： Lelong IH,Cardarelli CO,Gottesman MM,Pastan I

更新日期：1994-08-02 00:00:00

abstract：:Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...

journal_title：Biochemistry

authors： Quantin B,Murphy G,Breathnach R

更新日期：1989-06-27 00:00:00

abstract：:Protein phosphatase 1 (PP1) is an essential and ubiquitous serine/threonine protein phosphatase that is regulated by more than 100 known inhibitor and targeting proteins. It is currently unclear how protein inhibitors distinctly and specifically regulate PP1 to enable rapid responses to cellular alterations. We demons...

journal_title：Biochemistry

authors： Dancheck B,Nairn AC,Peti W

更新日期：2008-11-25 00:00:00

abstract：:Genetic studies have previously demonstrated that the Saccharomyces cerevisiae CDC9 gene product, which is functionally homologous to mammalian DNA ligase I, is required for DNA replication and is also involved in DNA repair and genetic recombination. In the present study we have purified the yeast enzyme. When measur...

journal_title：Biochemistry

authors： Tomkinson AE,Tappe NJ,Friedberg EC

更新日期：1992-12-01 00:00:00

abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

journal_title：Biochemistry

authors： Sha R,Liu F,Iwasaki H,Seeman NC

更新日期：2002-09-10 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are a family of enzymes that are up-regulated in many diseases, including osteoarthritis (OA) and rheumatoid arthritis (RA). Here we report on a novel technique that can be used to simultaneously measure activity levels for a panel of enzymes, such as the MMPs. The technique, termed th...

journal_title：Biochemistry

authors： Rasmussen FH,Yeung N,Kiefer L,Murphy G,Lopez-Otin C,Vitek MP,Moss ML

更新日期：2004-03-23 00:00:00

abstract：:Rabbit lung flavin-containing monooxygenase (FMO, EC 1.14.13.8) was denatured, reduced, carboxymethylated, digested with endoproteinase Glu-C or trypsin, and subjected to mass spectrometric analysis. The amino acid sequences of selected peptides were determined by tandem mass spectrometry. Over 90% of rabbit lung FMO ...

journal_title：Biochemistry

authors： Guan SH,Falick AM,Williams DE,Cashman JR

更新日期：1991-10-15 00:00:00

abstract：:The active site structures of human Q31 granzyme A, murine granzymes (A, B, C, D, E, and F), and human granzymes (A, B, and 3) isolated from cytotoxic T lymphocytes (CTL) were studied with peptide thioester substrates, peptide chloromethyl ketone, and isocoumarin inhibitors. Human Q31, murine, and human granzyme A hyd...

journal_title：Biochemistry

authors： Odake S,Kam CM,Narasimhan L,Poe M,Blake JT,Krahenbuhl O,Tschopp J,Powers JC

更新日期：1991-02-26 00:00:00

abstract：:The EcoRV restriction/modification system consists of two enzymes that recognize the DNA sequence GATATC. The EcoRV restriction endonuclease cleaves DNA at this site, but the DNA of Escherichia coli carrying the EcoRV system is protected from this reaction by the EcoRV methyltransferase. However, in vitro, the EcoRV n...

journal_title：Biochemistry

authors： Taylor JD,Goodall AJ,Vermote CL,Halford SE

更新日期：1990-12-04 00:00:00

abstract：:The substrate-bound form of the enzyme heme oxygenase (HO), which catalyzed the stereospecific alpha-meso bridge cleavage of hemin to yield biliverdin IX alpha, has been investigated by 1H NMR in both its primarily high-spin and its cyanide-inhibited low-spin forms. Both derivatives yield 1H NMR spectra indicative of ...

journal_title：Biochemistry

authors： Hernández G,Wilks A,Paolesse R,Smith KM,Ortiz de Montellano PR,La Mar GN

更新日期：1994-05-31 00:00:00

abstract：:Mutagenesis and cross-linking measurements have identified specific contact interactions between the cytosolic and the transmembrane sequences of phospholamban (PLB) and the Ca-ATPase, and in conjunction with the high-resolution structures of PLB and the Ca-ATPase, have been used to construct models of the PLB-ATPase ...

journal_title：Biochemistry

authors： Li J,Xiong Y,Bigelow DJ,Squier TC

更新日期：2004-01-20 00:00:00

abstract：:Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acy...

journal_title：Biochemistry

authors： Ford DA,Horner CC,Gross RW

更新日期：1998-08-25 00:00:00

abstract：:S-Adenosylmethionine (AdoMet) synthetase catalyzes the only known route of biosynthesis of the primary in vivo alkylating agent. Inhibitors of this enzyme could provide useful modifiers of biological methylation and polyamine biosynthetic processes. The AdoMet synthetase catalyzed reaction converts ATP and L-methionin...

journal_title：Biochemistry

authors： Reczkowski RS,Markham GD

更新日期：1999-07-13 00:00:00

abstract：:The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-32P]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with in...

journal_title：Biochemistry

authors： Carlson BJ,Raftery MA

更新日期：1993-07-27 00:00:00

abstract：:CTP synthetase [EC 6.3.4.2, UTP:ammonia ligase (ADP-forming)] from the yeast Saccharomyces cerevisiae catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to the C-4 position of UTP to form CTP. In this work, we demonstrated that CTP synthetase utilized dUTP as a substrate to synthesize dCTP. The ...

journal_title：Biochemistry

authors： Pappas A,Park TS,Carman GM

更新日期：1999-12-14 00:00:00

abstract：:The purpose of this study was to investigate the molecular action of lithocholic acid (LCA), known as a selective inhibitor of DNA polymerase beta (pol beta). The 39-kDa pol beta was separated proteolytically into two fragments of the template-primer binding domain (8 kDa) and the catalytic domain (31 kDa). LCA bound ...

journal_title：Biochemistry

authors： Mizushina Y,Ohkubo T,Sugawara F,Sakaguchi K

更新日期：2000-10-17 00:00:00

abstract：:Biological membranes exhibit an asymmetric distribution of phospholipids. Phosphatidylserine (PS) is an acidic phospholipid that is found almost entirely on the interior of the cell where it is important for interaction with many cellular components. A less well understood phenomenon is the asymmetry of the neutral ph...

journal_title：Biochemistry

authors： Bazzi MD,Youakim MA,Nelsestuen GL

更新日期：1992-02-04 00:00:00

abstract：:The second-order rate constants for the reactions between nitrogen monoxide and oxymyoglobin or oxyhemoglobin, determined by stopped-flow spectroscopy, increase with increasing pH. At pH 7.0 the rates are (43.6 +/- 0.5) x 10(6) M(-1) x s(-1) for oxymyoglobin and (89 +/- 3) x 10(6) M(-1) x s(-1) for oxyhemoglobin (per ...

journal_title：Biochemistry

authors： Herold S,Exner M,Nauser T

更新日期：2001-03-20 00:00:00

abstract：:A recently discovered bifunctional antibiotic-resistance enzyme named AAC(3)-Ib/AAC(6')-Ib', from Pseudomonas aeruginosa, catalyzes acetylation of aminoglycoside antibiotics. Since both domains are acetyltransferases, each was cloned and purified for mechanistic studies. The AAC(3)-Ib domain appears to be highly speci...

journal_title：Biochemistry

authors： Kim C,Villegas-Estrada A,Hesek D,Mobashery S

更新日期：2007-05-01 00:00:00

abstract：:Protein methylation is a common post-translational modification with diverse biological functions. Methyllysine reader proteins are increasingly a focus of epigenetics research and play important roles in regulating many cellular processes. These reader proteins are vital players in development, cell cycle regulation,...

journal_title：Biochemistry

authors： Milosevich N,Hof F

更新日期：2016-03-22 00:00:00

abstract：:RNAs are involved in myriad cellular events. In general, RNA function is affected by cellular conditions. For instance, molecular crowding promotes RNA folding through compaction of the RNA. Metabolites generally destabilize RNA secondary structure, which improves RNA folding cooperativity and increases the fraction o...

journal_title：Biochemistry

authors： Yamagami R,Huang R,Bevilacqua PC

更新日期：2019-09-24 00:00:00

abstract：:The influence of structural alterations to the estradiol-17 beta (E2) molecule on the induction of pS2 and Cathepsin D (Cath D) mRNAs has been examined by Northern analysis of RNA extracted from MCF-7 cells. Exposure of cultures to estratriene did not affect the level of expression of these estrogen-responsive genes. ...

journal_title：Biochemistry

authors： Pilat MJ,Hafner MS,Kral LG,Brooks SC

更新日期：1993-07-13 00:00:00

abstract：:Lipocortin I is a 39-kilodalton membrane-associated protein that in A431 cells is phosphorylated on tyrosine in response to epidermal growth factor (EGF). We have used recombinant human lipocortin I as a substrate for several protein kinases and identified phosphorylated residues by a combination of peptide mapping an...

journal_title：Biochemistry

authors： Varticovski L,Chahwala SB,Whitman M,Cantley L,Schindler D,Chow EP,Sinclair LK,Pepinsky RB

更新日期：1988-05-17 00:00:00

abstract：:The function of Lys-59, Arg-141, and Arg-277 in PAPS binding and catalysis of the flavonol 3-sulfotransferase was investigated. Affinity chromatography of conservative mutants with PAPS analogues allowed us to determine that Lys-59 interacts with the 5' portion of the nucleotide, while Arg-141 interacts with the 3' po...

journal_title：Biochemistry

authors： Marsolais F,Laviolette M,Kakuta Y,Negishi M,Pedersen LC,Auger M,Varin L

更新日期：1999-03-30 00:00:00