23,24,25-Trihydroxyvitamin D3, 24,25,26-trihydroxyvitamin D3, 24-keto-25-hydroxyvitamin D3, and 23-dehydro-25-hydroxyvitamin D3: new in vivo metabolites of vitamin D3.

abstract：:The oxidation of deoxy- and oxyhemerythrin to methemerythrin by Fe(CN)6(3-) has been examined. The first step produces (semi-met)O, a half-oxidized form of hemerythrin which has different spectral and kinetic properties from (semi-met)R which is produced by one-electron reduction of methemerythrin. Further oxidation o...

journal_title：Biochemistry

authors： Bradić Z,Harrington PC,Wilkins RG,Yoneda G

更新日期：1980-09-02 00:00:00

abstract：:Phenylalanine hydroxylase (PheH) and tryptophan hydroxylase (TrpH) catalyze the aromatic hydroxylation of phenylalanine and tryptophan, forming tyrosine and 5-hydroxytryptophan, respectively. The reactions of PheH and TrpH have been investigated with [4-(2)H]-, [3,5-(2)H(2)]-, and (2)H(5)-phenylalanine as substrates. ...

journal_title：Biochemistry

authors： Pavon JA,Fitzpatrick PF

更新日期：2006-09-12 00:00:00

abstract：:Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...

journal_title：Biochemistry

authors： Oberdorf J,Carlson EJ,Skach WR

更新日期：2001-11-06 00:00:00

abstract：:Hydrophobic interactions are responsible for stabilizing leucine zippers in peptides containing heptad repeats. The effects of substituting leucine by phenylalanine and alanine by glycine on the self-assembly of coiled-coils were examined in minimalist antimicrobial peptides designed to form amphipathic alpha-helices....

journal_title：Biochemistry

authors： Javadpour MM,Barkley MD

更新日期：1997-08-05 00:00:00

abstract：:In mature human apolipoprotein A-I (apo A-I), the amino acid residues 1-43 are encoded by exon 3, whereas residues 44-243 are encoded by exon 4 of the apo A-I gene. The region encoded by exon 4 of the apo A-I gene contains 10 tandem amphipathic alpha-helixes; their location and the class to which they belong are as fo...

journal_title：Biochemistry

authors： Mishra VK,Palgunachari MN,Datta G,Phillips MC,Lund-Katz S,Adeyeye SO,Segrest JP,Anantharamaiah GM

更新日期：1998-07-14 00:00:00

abstract：:The solution conformation of the type I collagen alpha-1 chain N-telopeptide has been studied by CD and 1H NMR spectroscopy at 600 MHz in CD3OH/H2O (60/40 v/v) and H2O solutions. The 19 amino acids form the N-terminal end of the alpha-1 polypeptide chain. By the combined application of several two-dimensional, phase-s...

journal_title：Biochemistry

authors： Otter A,Kotovych G,Scott PG

更新日期：1989-10-03 00:00:00

abstract：:All eight cysteine residues, C90, C94, C143, C147, C219, C325, C367, and C431, present in transmembrane domains of the Aspergillus nidulans NrtA nitrate transporter protein were altered individually by site-specific mutagenesis. The results indicate that six residues, C90, C147, C219, C325, C367, and C431, are not req...

journal_title：Biochemistry

authors： Unkles SE,Rouch DA,Wang Y,Siddiqi MY,Okamoto M,Stephenson RM,Kinghorn JR,Glass AD

更新日期：2005-04-12 00:00:00

abstract：:It is now appreciated that the relationships of proteins, particularly enzymes, within a protein superfamily can be understood not only in terms of their sequence similarities and three-dimensional structures but also by chemical threads that relate their functional attributes. The mechanistic ties among superfamily m...

journal_title：Biochemistry

authors： Armstrong RN

更新日期：2000-11-14 00:00:00

abstract：:The proton nuclear Overhauser effects of bovine alpha-lactalbumin were studied at 200 MHz by irradiation of an upfield ring current shifted methylene at -2.45 ppm (assigned to Ile-95) and two aromatic protons, Tyr-103 (8.36 ppm) and Trp-60 (5.85 ppm). The experimental results were consistent with a putative three-dime...

journal_title：Biochemistry

authors： Koga K,Berliner LJ

更新日期：1985-12-03 00:00:00

abstract：:We have used enzymic and chemical probes to search for altered DNA conformations in the 5' flanking region of the gene for a high mobility group protein (HMG-T) from trout. This search was conducted in order to identify potential genetic elements that might be involved in the transcriptional control of the HMG-T gene....

journal_title：Biochemistry

authors： Wright JM,Dixon GH

更新日期：1988-01-26 00:00:00

abstract：:One of the most promising approaches to anti-hepatitis C virus drug discovery is the development of inhibitors of the virally encoded protease NS3. This chymotrypsin-like serine protease is essential for the maturation of the viral polyprotein, and processing requires complex formation between NS3 and its cofactor NS4...

journal_title：Biochemistry

authors： Bianchi E,Orrù S,Dal Piaz F,Ingenito R,Casbarra A,Biasiol G,Koch U,Pucci P,Pessi A

更新日期：1999-10-19 00:00:00

abstract：:The 270-MHz 1H nuclear magnetic resonance spectra of the apo, copper(I)-zinc(II), and copper(II)-zinc(II) forms of bovine erythrocyte superoxide dismutase (EC 1.15.1.1) are reported, and assignments of resonances to ten amino acid residues are proposed. The data require that at least four and probably six histidine re...

journal_title：Biochemistry

authors： Cass AE,Hill AO,Smith BE,Bannister JV,Bannister WH

更新日期：1977-07-12 00:00:00

abstract：:Ribonucleotide reductase from Escherichia coli consists of two nonidentical subunits, named protein B1 (170 000) and protein B2 (87 000). We purified and characterized five monoclonal antibodies against B1 and three against B2 from hybridomas obtained by fusion of spleen cells from immunized mice and the myeloma cell ...

journal_title：Biochemistry

authors： Anderson A,Barlow T,Pontis E,Reichard P

更新日期：1986-02-25 00:00:00

abstract：:The dependence of the activity of recombinant activated human protein C (r-APC) on each of its nine gamma-carboxyglutamic (Gla) residues (sequence positions 6, 7, 14, 16, 19, 20, 25, 26, and 29) has been assessed in purified systems and in plasma using r-mutants in which each Gla residue of r-APC was individually alte...

journal_title：Biochemistry

authors： Jhingan A,Zhang L,Christiansen WT,Castellino FJ

更新日期：1994-02-22 00:00:00

abstract：:Studies of the mechanism of protein secretion in a Gram-positive bacterium, Bacillus subtilis, yielded results very similar to those previously obtained with a Gram-negative organism: nascent chains protruding from protoplasts could be labeled extracellularly; the labeled chains could be recovered on polysomes isolate...

journal_title：Biochemistry

authors： Smith WP,Tai PC,Davis BD

更新日期：1979-01-09 00:00:00

abstract：:Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...

journal_title：Biochemistry

authors： Cook PF,Yoon MY,Hara S,McClure GD Jr

更新日期：1993-02-23 00:00:00

abstract：:The P22 tailspike endorhamnosidase confers the high specificity of bacteriophage P22 for some serogroups of Salmonella differing only slightly in their O-antigen polysaccharide. We used several biophysical methods to study the binding and hydrolysis of O-antigen fragments of different lengths by P22 tailspike protein....

journal_title：Biochemistry

authors： Baxa U,Weintraub A,Seckler R

更新日期：2020-12-29 00:00:00

abstract：:Penicillin-binding protein 5 (PBP 5) from Escherichia coli is a well-characterized d-alanine carboxypeptidase that serves as a prototypical enzyme to elucidate the structure, function, and catalytic mechanism of PBPs. A comprehensive understanding of the catalytic mechanism underlying d-alanine carboxypeptidation and ...

journal_title：Biochemistry

authors： Nicola G,Peddi S,Stefanova M,Nicholas RA,Gutheil WG,Davies C

更新日期：2005-06-14 00:00:00

abstract：:Base excision repair (BER) is the major corrective pathway for most spontaneous, oxidative, and alkylation DNA base and sugar damage. X-ray cross-complementing 1 (XRCC1) has been suggested to function at nearly every step of this repair process, primarily through direct protein-protein interactions. Using whole cell e...

journal_title：Biochemistry

authors： Wong HK,Kim D,Hogue BA,McNeill DR,Wilson DM 3rd

更新日期：2005-11-01 00:00:00

abstract：:CYP2C19 is selective for the 4'-hydroxylation of S-mephenytoin while the highly similar CYP2C9 has little activity toward this substrate. To identify critical amino acids determining the specificity of human CYP2C19 for S-mephenytoin 4'-hydroxylation, we constructed chimeras by replacing portions of CYP2C9 containing ...

journal_title：Biochemistry

authors： Tsao CC,Wester MR,Ghanayem B,Coulter SJ,Chanas B,Johnson EF,Goldstein JA

更新日期：2001-02-20 00:00:00

abstract：:By suspending bovine rhodopsin in trehalose-water glass films, it is possible to trap photostates in the light-activation process. Because of the unusually high vitrification temperature of trehalose-water mixtures, this trapping can be accomplished at room temperature. This allows for a facile investigation of the sp...

journal_title：Biochemistry

authors： Sikora S,Little AS,Dewey TG

更新日期：1994-04-19 00:00:00

abstract：:To identify the residues essential for interfacial binding and substrate binding of human pancreatic phospholipase A2 (hpPLA2), several ionic residues in the putative interfacial binding surface (R6E, K7E, K10E, and K116E) and substrate binding site (D53K and K56E) were mutated. Interfacial affinity of these mutants w...

journal_title：Biochemistry

authors： Snitko Y,Han SK,Lee BI,Cho W

更新日期：1999-06-15 00:00:00

abstract：:Electronic absorption and resonance Raman spectra, together with rates of arsenolysis, are used to show that the acylated subunits of the rabbit furylacryloylglyceraldehyde-3-phosphate dehydrogenase (FA-rabbit enzyme), but not the FA-sturgeon enzyme, exist as a mixed population of at least two forms. One form of the F...

journal_title：Biochemistry

authors： Storer AC,Phelps DJ,Carey PR

更新日期：1981-06-09 00:00:00

abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...

journal_title：Biochemistry

authors： Maddukuri L,Shuck SC,Eoff RL,Zhao L,Rizzo CJ,Guengerich FP,Marnett LJ

更新日期：2013-12-03 00:00:00

abstract：:The bacterial alpha-lytic protease (alphaLP) is synthesized as a precursor containing a large N-terminal pro region (Pro) transiently required for correct folding of the protease [Silen, J. L., and Agard, D. A. (1989) Nature 341, 462-464]. Upon folding, the precursor is autocatalyticly cleaved to yield a tight-binding...

journal_title：Biochemistry

authors： Anderson DE,Peters RJ,Wilk B,Agard DA

更新日期：1999-04-13 00:00:00

abstract：:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzy...

journal_title：Biochemistry

authors： Halling SM,Sanchez-Anzaldo FJ,Fukuda R,Doi RH,Meares CF

更新日期：1977-06-28 00:00:00

abstract：:Aquaporin-4 (AQP4) is the predominant water channel in the brain. Although AQP4 plays an important role in brain water homeostasis, the molecular mechanisms of AQP4 regulation are not fully understood. In this report, we show how Zn(2+) rapidly and reversibly decreases the water permeability of AQP4 when it is reconst...

journal_title：Biochemistry

authors： Yukutake Y,Hirano Y,Suematsu M,Yasui M

更新日期：2009-12-29 00:00:00

abstract：:The active form of the animal fatty acid synthase (FAS) is a dimer of identical multifunctional polypeptides, each containing seven discrete functional domains, that cooperate to form two centers for palmitate synthesis. To assess the importance of domain cooperation across the subunit interface in the reaction mechan...

journal_title：Biochemistry

authors： Joshi AK,Witkowski A,Smith S

更新日期：1998-02-24 00:00:00

abstract：:The three-dimensional structure of the bifunctional alpha-amylase/trypsin inhibitor (RBI) from seeds of ragi (Eleusine coracana Gaertneri) has been determined in solution using multidimensional 1H and 15N NMR spectroscopy. The inhibitor consists of 122 amino acids, with 5 disulfide bridges, and belongs to the plant al...

journal_title：Biochemistry

authors： Strobl S,Mühlhahn P,Bernstein R,Wiltscheck R,Maskos K,Wunderlich M,Huber R,Glockshuber R,Holak TA

更新日期：1995-07-04 00:00:00

abstract：:The biosynthesis of epothilones, a family of hybrid polyketide (PK)/nonribosomal peptide (NRP) antitumor agents, provides an ideal system to study a hybrid PK/NRP natural product with significant biomedical value. Here the third enzyme involved in epothilone production, the five domain 195 kDa polyketide synthase (PKS...

journal_title：Biochemistry

authors： O'Connor SE,Chen H,Walsh CT

更新日期：2002-04-30 00:00:00