当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Replication, repair, and translesion polymerase bypass of N⁶-oxopropenyl-2'-deoxyadenosine.

Replication, repair, and translesion polymerase bypass of N⁶-oxopropenyl-2'-deoxyadenosine.

Abstract：

:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been done on OPdA. To improve our understanding of the effect of OPdA on polymerase activity and mutagenicity, we evaluated the ability of the translesion DNA polymerases hPols η, κ, and ι to bypass OPdA in vitro. hPols η and κ inserted dNTPs opposite the lesion and extended the OPdA-modified primer to the terminus. hPol ι inserted dNTPs opposite OPdA but failed to fully extend the primer. Steady-state kinetic analysis indicated that these polymerases preferentially insert dTTP opposite OPdA, although less efficiently than opposite dA. Minimal incorrect base insertion was observed for all polymerases, and dCTP was the primary mis-insertion event. Examining replicative and repair polymerases revealed little effect of OPdA on the Sulfolobus solfataricus polymerase Dpo1 or the Klenow fragment of Escherichia coli DNA polymerase I. Bacteriophage T7 DNA polymerase displayed a reduced level of OPdA bypass compared to unmodified DNA, and OPdA nearly completely blocked the activity of base excision repair polymerase hPol β. This work demonstrates that bypass of OPdA is generally error-free, modestly decreases the catalytic activity of most polymerases, and blocks hPol β polymerase activity. Although mis-insertion opposite OPdA is relatively weak, the efficiency of bypass may introduce A → G transitions observed in vivo.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Maddukuri L,Shuck SC,Eoff RL,Zhao L,Rizzo CJ,Guengerich FP,Marnett LJ

doi

10.1021/bi401103k

subject

Has Abstract

pub_date

2013-12-03 00:00:00

pages

8766-76

issue

48

eissn

0006-2960

issn

1520-4995

journal_volume

52

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Ion concentration and temperature dependence of DNA binding: comparison of PurR and LacI repressor proteins.

    abstract：:Purine repressor (PurR) binding to specific DNA is enhanced by complexing with purines, whereas lactose repressor (LacI) binding is diminished by interaction with inducer sugars despite 30% identity in their protein sequences and highly homologous tertiary structures. Nonetheless, in switching from low- to high-affini...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0028643

    authors： Moraitis MI,Xu H,Matthews KS

    更新日期：2001-07-10 00:00:00

  • The selectivity for K+ versus Na+ in DNA quadruplexes is dominated by relative free energies of hydration: a thermodynamic analysis by 1H NMR.

    abstract：:We have studied the competition between Na+ and K+ for coordination by G quartets using the oligonucleotide d(G3T4G3) as a model system. d(G3T4G3) forms a dimeric foldback structure containing three G quartets in the presence of either NaCl or KCl. Proton chemical shifts, which are particular to the species of coordin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9620565

    authors： Hud NV,Smith FW,Anet FA,Feigon J

    更新日期：1996-12-03 00:00:00

  • Kinetic mechanism of deoxyadenosine kinase from Mycoplasma determined by surface plasmon resonance technology.

    abstract：:Surface plasmon resonance (SPR) detection technology was employed to investigate the kinetic mechanism of deoxyadenosine kinase from Mycoplasma mycoides ssp. mycoides SC. In our experimental approach, the enzyme was attached to the sensor surface, the reactants were injected in the mobile phase, and the product-enzyme...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0515523

    authors： Pol E,Wang L

    更新日期：2006-01-17 00:00:00

  • Biochemical studies on Mycobacterium tuberculosis UreG and comparative modeling reveal structural and functional conservation among the bacterial UreG family.

    abstract：:Nickel is a fundamental micronutrient for cellular life, but it is toxic in soluble form at nonphysiological concentrations. Such potentially contradictory features required living organisms to develop efficient systems for nickel utilization and homeostasis. This is the case for incorporation of nickel into the activ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi6024676

    authors： Zambelli B,Musiani F,Savini M,Tucker P,Ciurli S

    更新日期：2007-03-20 00:00:00

  • Influence of cofactor pyridoxal 5'-phosphate on reversible high-pressure denaturation of isolated beta 2 dimer of tryptophan synthase bienzyme complex from Escherichia coli.

    abstract：:High hydrostatic pressure has been shown to cause reversible dissociation of the isolated apo beta 2 dimer of tryptophan synthase from Escherichia coli into enzymatically inactive monomers [Seifert, T., Bartholmes, P., & Jaenicke, R. (1982) Biophys. Chem. 15, 1-8]. Addition of the coenzyme pyridoxal 5'-phosphate affec...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00323a016

    authors： Seifert T,Bartholmes P,Jaenicke R

    更新日期：1985-01-15 00:00:00

  • Evidence that the FX domain in photosystem I interacts with the subunit PsaC: site-directed changes in PsaB destabilize the subunit interaction in Chlamydomonas reinhardtii.

    abstract：:The highly conserved amino acid sequence PCDGPGRGGTC in both photosystem I reaction center core proteins PsaA and PsaB has been predicted to contribute the four cysteine ligands for coordination of the 4Fe-4S iron-sulfur cluster FX, and we have proposed a working model for the binding of PsaC to this domain of the rea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00019a010

    authors： Rodday SM,Webber AN,Bingham SE,Biggins J

    更新日期：1995-05-16 00:00:00

  • Characterization of the interaction between androgen receptor and a new transcriptional inhibitor, SHP.

    abstract：:SHP (short heterodimer partner) is an orphan nuclear receptor, first described for its interaction with nuclear receptors. This study explores a new way of inhibiting the androgen-signaling pathway. We demonstrated that SHP inhibited up to 97% of AR-induced activity. Characterization of AR/SHP interaction provided evi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi011384o

    authors： Gobinet J,Auzou G,Nicolas JC,Sultan C,Jalaguier S

    更新日期：2001-12-18 00:00:00

  • Chromatographic separation on phosphocellulose of activated and nonactivated forms of steroid-receptor complex. Purification of the activated complex.

    abstract：:Steroid-receptor complexes formed at low temperature and ionic strength are unable to bind to target cell nuclei. After a temporary exposure to high ionic strength and/or temperature they become activated (i.e., able to bind to nuclei). However, there exists an equilibrium between activated and nonactivated complexes;...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00664a025

    authors： Atger M,Milgrom E

    更新日期：1976-09-21 00:00:00

  • Subunit dissociation as a possible mechanism of glucocorticoid receptor activation.

    abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00277a038

    authors： Vedeckis WV

    更新日期：1983-04-12 00:00:00

  • Evaluation of copper2+ affinities for the prion protein.

    abstract：:The prion protein (PrP) is a cell-surface Cu2+ binding glycoprotein which can bind six copper ions. The role of Cu2+ in PrP function, misfolding, and prion disease has generated much interest; however, the field has been hampered by a lack of consensus with regard to the affinity of Cu2+ for PrPC. Here we build on our...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9011397

    authors： Nadal RC,Davies P,Brown DR,Viles JH

    更新日期：2009-09-29 00:00:00

  • Glycolipid Biosurfactants Activate, Dimerize, and Stabilize Thermomyces lanuginosus Lipase in a pH-Dependent Fashion.

    abstract：:We present a study of the interactions between the lipase from Thermomyces lanuginosus (TlL) and the two microbially produced biosurfactants (BSs), rhamnolipid (RL) and sophorolipid (SL). Both RL and SL are glycolipids; however, RL is anionic, while SL is a mixture of anionic and non-ionic species. We investigate the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00420

    authors： Madsen JK,Kaspersen JD,Andersen CB,Nedergaard Pedersen J,Andersen KK,Pedersen JS,Otzen DE

    更新日期：2017-08-15 00:00:00

  • Ligand-induced heme ruffling and bent no geometry in ultra-high-resolution structures of nitrophorin 4.

    abstract：:The nitrophorins are a family of proteins that use ferric heme to transport nitric oxide (NO) from the salivary glands of blood-sucking insects to their victims, resulting in vasodilation and reduced blood coagulation. We have refined atomic resolution structures of nitrophorin 4 (NP4) from Rhodnius prolixus complexed...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0109257

    authors： Roberts SA,Weichsel A,Qiu Y,Shelnutt JA,Walker FA,Montfort WR

    更新日期：2001-09-25 00:00:00

  • Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods.

    abstract：:The complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) (EC 2.6.1.2) is presented. Two primary sets of overlapping fragments were obtained by cleavage of the pyridylethylated protein at methionyl and lysyl bonds with cyanogen bromide and Achromobacter protease I, respectively. Isolated...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00107a013

    authors： Ishiguro M,Takio K,Suzuki M,Oyama R,Matsuzawa T,Titani K

    更新日期：1991-10-29 00:00:00

  • DNA cross-linking by intermediates in the mitomycin activation cascade.

    abstract：:We have assayed the cross-linking of oligonucleotides containing repeated mitomycin-reactive CpG sites in order to assess the factors that enhance activation of the carbamoyl function at C10, yielding efficient mitomycin cross-linking. Drugs studied include mitomycin C (MC), N-methylmitomycin A (NMA), and the aziridin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00439a049

    authors： Cera C,Egbertson M,Teng SP,Crothers DM,Danishefsky SJ

    更新日期：1989-06-27 00:00:00

  • Biosynthetic incorporation of fluorescent carbazolylundecanoic acid into membrane phospholipids of LM cells and determination of quenching constants and partition coefficients of hydrophobic quenchers.

    abstract：:A fluorescence quenching method was developed for determining partition coefficients and diffusional rates of small molecules in cell membranes. This method involves quenching the fluorescence of carbazole-labeled membranes by hydrophobic molecules that partition into membranes. Cell membrane phospholipids of mouse LM...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00316a021

    authors： Omann GM,Glaser M

    更新日期：1984-10-09 00:00:00

  • 2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract.

    abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi062117r

    authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

    更新日期：2007-06-05 00:00:00

  • Elucidation of the chemical nature of the steady-state intermediates in the mechanism of carboxypeptidase A.

    abstract：:Cryospectrokinetic studies of zinc and cobalt carboxypeptidase A disclosed two intermediates in the hydrolysis of both peptides and depsipeptides and furnished all the rate and equilibrium constants for the reaction scheme E + S in equilibrium ES1 in equilibrium ES2---E + P [Auld, D. S., Galdes, A., Geoghegan, K. F., ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00351a020

    authors： Galdes A,Auld DS,Vallee BL

    更新日期：1986-02-11 00:00:00

  • Dynamic and equilibrium studies on the interaction of Ran with its effector, RanBP1.

    abstract：:Ran, a small nuclear GTP-binding protein, is one of the most abundant Ras-related proteins in eucaryotic cells. Ran is essential for nucleo-cytoplasmatic transport and is primarily localized in the nucleus and at the nuclear pore complex. Here, we characterize the kinetics and equilibrium of the interaction between Ra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970524k

    authors： Kuhlmann J,Macara I,Wittinghofer A

    更新日期：1997-10-07 00:00:00

  • Site-specific variations in RNA folding thermodynamics visualized by 2-aminopurine fluorescence.

    abstract：:The fluorescent base analogue 2-aminopurine (2-AP) is commonly used to study specific conformational and protein binding events involving nucleic acids. Here, combinations of steady-state and time-resolved fluorescence spectroscopy of 2-AP were employed to monitor conformational transitions within a model hairpin RNA ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7011977

    authors： Ballin JD,Bharill S,Fialcowitz-White EJ,Gryczynski I,Gryczynski Z,Wilson GM

    更新日期：2007-12-11 00:00:00

  • Identification from a phage display library of peptides that bind to toxic shock syndrome toxin-1 and that inhibit its binding to major histocompatibility complex (MHC) class II molecules.

    abstract：:Phage display technique is a powerful tool with which to identify novel binding sequences for antibody and receptor targets. Few studies, however, have used this technology to select affinity peptides for ligand molecules. Here, we screened a peptide phage library for binding to toxic shock syndrome toxin 1 (TSST-1) t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960132y

    authors： Sato A,Ida N,Fukuyama M,Miwa K,Kazami J,Nakamura H

    更新日期：1996-08-13 00:00:00

  • Oxygen yield and thermoluminescence characteristics of a cyanobacterium lacking the manganese-stabilizing protein of photosystem II.

    abstract：:Previous experiments have shown that a Synechocystis sp. PCC 6803 mutant (delta psbO) lacking the extrinsic manganese-stabilizing protein (MSP) exhibits impaired, but significant levels of H2O-splitting activity [Burnap, R., & Sherman, L.A. (1991) Biochemistry 30, 440-446]. [14C]DCMU-binding experiments now show that ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00147a027

    authors： Burnap RL,Shen JR,Jursinic PA,Inoue Y,Sherman LA

    更新日期：1992-08-18 00:00:00

  • Targeted generation of DNA strand breaks using pyrene-conjugated triplex-forming oligonucleotides.

    abstract：:Gene targeting by triplex-forming oligonucleotides (TFOs) has proven useful for gene modulation in vivo. Photoreactive molecules have been conjugated to TFOs to direct sequence-specific damage in double-stranded DNA. However, the photoproducts are often repaired efficiently in cells. This limitation has led to the sea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7024029

    authors： Benfield AP,Macleod MC,Liu Y,Wu Q,Wensel TG,Vasquez KM

    更新日期：2008-06-10 00:00:00

  • A protein arginine N-methyltransferase 1 (PRMT1) and 2 heteromeric interaction increases PRMT1 enzymatic activity.

    abstract：:Protein arginine N-methyltransferases (PRMTs) act in signaling pathways and gene expression by methylating arginine residues within target proteins. PRMT1 is responsible for most cellular arginine methylation activity and can work independently or in collaboration with other PRMTs. In this study, we demonstrate a dire...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200644c

    authors： Pak ML,Lakowski TM,Thomas D,Vhuiyan MI,Hüsecken K,Frankel A

    更新日期：2011-09-27 00:00:00

  • CO-induced structural rearrangement of the C cluster in Carboxydothermus hydrogenoformans CO dehydrogenase-evidence from Ni K-edge X-ray absorption spectroscopy.

    abstract：:We have examined the C cluster in type II CO dehydrogenase (CODH) from Carboxydothermus hydrogenformans using Ni K-edge X-ray absorption near edge spectroscopy and extended X-ray absorption fine structure (EXAFS) spectroscopy. The enzyme was studied under three conditions: "as-isolated" and after treatment with CO or ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi036104e

    authors： Gu W,Seravalli J,Ragsdale SW,Cramer SP

    更新日期：2004-07-20 00:00:00

  • Investigating Pharmacological Targeting of G-Quadruplexes in the Human Malaria Parasite.

    abstract：:The unique occurrence of G-quadruplexes in the AT-rich genome of human malaria parasite Plasmodium falciparum provides hints about their critical roles in parasite survival, pathogenesis, and host immune evasion. An intriguing question is whether these noncanonical structures can serve as molecular targets for small m...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00964

    authors： Anas M,Sharma R,Dhamodharan V,Pradeepkumar PI,Manhas A,Srivastava K,Ahmed S,Kumar N

    更新日期：2017-12-26 00:00:00

  • Replacement of the proximal ligand of sperm whale myoglobin with free imidazole in the mutant His-93-->Gly.

    abstract：:The proximal bond between the iron atom of the heme group and the N epsilon of histidine F8 in myoglobin (Mb) and hemoglobin (Hb) is presumed to be an important determinant of heme binding, protein structure, and oxygen binding. Here a system is described in which the proximal ligand is provided intermolecularly by th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00187a023

    authors： Barrick D

    更新日期：1994-05-31 00:00:00

  • Disulfide structure of the leucine-rich repeat C-terminal cap and C-terminal stalk region of Nogo-66 receptor.

    abstract：:Nogo-66 receptor (NgR1) is a leucine-rich repeat (LRR) protein that forms part of a signaling complex modulating axon regeneration. Previous studies have shown that the entire LRR region of NgR1, including the C-terminal cap of the LRR, LRRCT, is needed for ligand binding, and that the adjacent C-terminal region (CT s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0517483

    authors： Wen D,Wildes CP,Silvian L,Walus L,Mi S,Lee DH,Meier W,Pepinsky RB

    更新日期：2005-12-20 00:00:00

  • Hydrolysis of tri- and monoacylglycerol by lipoprotein lipase: evidence for a common active site.

    abstract：:The relationship between triacylglycerol and monoacylglycerol hydrolyzing activities of purified rat heart lipoprotein lipase was studied using emulsified trioleoylglycerol and micellar or albumin-bound monooleoylglycerol as substrates. The maximal reaction rates obtained with the two substrates were similar (650 and ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00654a017

    authors： Twu JS,Nilsson-Ehle P,Schotz MC

    更新日期：1976-05-04 00:00:00

  • A new variant of the anion transport protein in human erythrocytes.

    abstract：:The major plasma membrane protein of human erythrocytes is the anion transport protein, termed protein 3. We previously reported a variant form of protein 3 that is elongated on the amino-terminal end of the molecule, which is exposed on the cytoplasmic side of the membrane, but otherwise its features are identical wi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00334a003

    authors： Hsu L,Morrison M

    更新日期：1985-06-18 00:00:00

  • Seipin is a discrete homooligomer.

    abstract：:Seipin is a transmembrane protein that resides in the endoplasmic reticulum and concentrates at junctions between the ER and cytosolic lipid droplets. Mutations in the human seipin gene, including the missense mutation A212P, lead to congenital generalized lipodystrophy (CGL), characterized by the lack of normal adipo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi1013003

    authors： Binns D,Lee S,Hilton CL,Jiang QX,Goodman JM

    更新日期：2010-12-21 00:00:00