Mechanistic diversity in a metalloenzyme superfamily.

abstract：:The gene 5 protein (g5p) encoded by filamentous Ff phages is an ssDNA-binding protein, which binds to and sequesters the nascent ssDNA phage genome in the process of phage morphogenesis. The g5p also binds with high affinity to DNA and RNA sequences that form G-quadruplex structures. However, sequences that would form...

journal_title：Biochemistry

authors： Wen JD,Gray DM

更新日期：2004-03-09 00:00:00

abstract：:The structures of oligosaccharides of normal and pathological immunoglobulin G (IgG) are reported. Asparagine-linked neutral oligosaccharides were released by N-oligosaccharide glycopeptidase (almond) digestion. The reducing ends of the oligosaccharide chains thus obtained were aminated with a fluorescent reagent, 2-a...

journal_title：Biochemistry

authors： Takahashi N,Ishii I,Ishihara H,Mori M,Tejima S,Jefferis R,Endo S,Arata Y

更新日期：1987-02-24 00:00:00

abstract：:Deposition of misfolded alpha-synuclein is a critical factor in several neurodegenerative disorders. Filamentous alpha-synuclein is the major component of Lewy bodies and Lewy neurites, the intracellular inclusions in the dopaminergic neurons of the substantia nigra, which are considered the pathological hallmark of P...

journal_title：Biochemistry

authors： Munishkina LA,Henriques J,Uversky VN,Fink AL

更新日期：2004-03-23 00:00:00

abstract：:In this work we modeled the circular dichroism (CD) spectrum of LHCII, the main light harvesting antenna of photosystem II of higher plants. Excitonic calculations are performed for a monomeric subunit, taken from the crystal structure of trimeric LHCII from spinach [Liu, Z. F., Yan, H. C., Wang, K. B., Kuang, T. Y., ...

journal_title：Biochemistry

authors： Georgakopoulou S,van der Zwan G,Bassi R,van Grondelle R,van Amerongen H,Croce R

更新日期：2007-04-24 00:00:00

abstract：:A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...

journal_title：Biochemistry

authors： Selden LA,Kinosian HJ,Estes JE,Gershman LC

更新日期：2000-01-11 00:00:00

abstract：:The ErbB family of tyrosine kinase receptors is a key element in preserving cell growth homeostasis. This family is comprised of four single-transmembrane domain proteins designated ErbB-1-4. Ligand binding initiates dimerization followed by tyrosine phosphorylation and signaling, which when uncontrolled lead to cance...

journal_title：Biochemistry

authors： Bublil EM,Cohen T,Arnusch CJ,Peleg A,Pines G,Lavi S,Yarden Y,Shai Y

更新日期：2016-10-04 00:00:00

abstract：:The sequence of events involved in poly(ethylene glycol)-mediated fusion of small unilamellar vesicles (SUVs) has been studied. Fusion events were monitored using light scattering for vesicle aggregation, the fluorescence lifetime of membrane probe lipids (DPHpPC and NBD-PS) for membrane mixing, the aqueous fluorescen...

journal_title：Biochemistry

authors： Lee J,Lentz BR

更新日期：1997-05-27 00:00:00

abstract：:Oligomerization of numerous G protein-coupled receptors has been documented, including the prototypic family B secretin receptor. The clinical significance of oligomerization of this receptor became clear with the recent observation that a misspliced form present in pancreatic cancer could associate with the wild-type...

journal_title：Biochemistry

authors： Lisenbee CS,Miller LJ

更新日期：2006-07-11 00:00:00

abstract：:Escherichia coli helicase II (UvrD) protein can initiate unwinding of duplex DNA at blunt ends or nicks, although these reactions require excess protein. We have undertaken kinetic studies of these reactions in order to probe the mechanism of initiation of unwinding. DNA unwinding was monitored directly by using agaro...

journal_title：Biochemistry

authors： Runyon GT,Lohman TM

更新日期：1993-04-20 00:00:00

abstract：:Endothelium-derived epoxyeicosatrienoic acids (EETs) relax vascular smooth muscle by activating potassium channels and causing membrane hyperpolarization. Recent evidence suggests that EETs act via a membrane binding site or receptor. To further characterize this binding site or receptor, we synthesized 20-iodo-14,15-...

journal_title：Biochemistry

authors： Chen Y,Falck JR,Manthati VL,Jat JL,Campbell WB

更新日期：2011-05-10 00:00:00

abstract：:The association of membrane and cytoplasmic proteins with the cytoskeleton of resting and activated platelets was studied. Glycoproteins were identified by labeling with 125I-labeled lectins (concanavalin A, wheat germ agglutinin, and Lens culinaris). Polypeptides, which are embedded in the lipid bilayer, have been id...

journal_title：Biochemistry

authors： Rotman A,Heldman J,Linder S

更新日期：1982-04-13 00:00:00

abstract：:The relationship between cholesterol and ubiquinone synthesis in rat intestinal epithelial cell cultures was examined by using 3 beta-[2-(diethylamino)ethoxy]androst-5-en-17-one hydrochloride (U18666A). Addition of U18666A to cells caused a greater than 90% inhibition of incorporation of [3H]acetate into cholesterol a...

journal_title：Biochemistry

authors： Sexton RC,Panini SR,Azran F,Rudney H

更新日期：1983-12-06 00:00:00

abstract：:Bovine milk lipoprotein lipase (LpL) catalyzes the hydrolysis of the water-soluble esters p-nitrophenyl acetate (PNPA) and p-nitrophenyl butyrate (PNPB). The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol h...

journal_title：Biochemistry

authors： Quinn DM,Shirai K,Jackson RL,Harmony JA

更新日期：1982-12-21 00:00:00

abstract：:The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosX(Ml)) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activi...

journal_title：Biochemistry

authors： Brown DW,Schaab MR,Birmingham WR,Armstrong RN

更新日期：2009-03-10 00:00:00

abstract：:Serine palmitoyltransferase (EC 2.3.1.50) catalyzes the condensation of L-serine and palmitoyl-CoA to yield 3-ketosphinganine in the first unique reaction of long-chain (sphingoid) base biosynthesis. The kinetic effects of changing the extracellular concentrations of the precursors for this pathway were studied with L...

journal_title：Biochemistry

authors： Merrill AH Jr,Wang E,Mullins RE

更新日期：1988-01-12 00:00:00

abstract：:In this paper we report the first 1H NMR study of the base-paired secondary structure of yeast 5.8S RNA. On the basis of a combination of homonuclear Overhauser enhancements and temperature dependence of the proton 500-MHz NMR spectrum, we are able to identify and assign eight of the nine base pairs in the most therma...

journal_title：Biochemistry

authors： Lee KM,Marshall AG

更新日期：1986-12-16 00:00:00

abstract：:There are four isoforms of centrin in mammals, with variable sequence, tissue expression, and functional properties. We have recently characterized a number of structural, ion, and target binding properties of human centrin isoform HsCen2. This paper reports a similar characterization of HsCen3, overexpressed in Esche...

journal_title：Biochemistry

authors： Cox JA,Tirone F,Durussel I,Firanescu C,Blouquit Y,Duchambon P,Craescu CT

更新日期：2005-01-25 00:00:00

abstract：:The crystallographic structure of oxygenated trHbN from Mycobacterium tuberculosis showed an extended heme distal site hydrogen-bonding network that includes Y(B10), Q(E11), and the bound O(2) (Milani, M., et al. (2001) EMBO J. 20, 3902-3909). In the present work, we analyze the effects that substitutions at the B10 a...

journal_title：Biochemistry

authors： Ouellet Y,Milani M,Couture M,Bolognesi M,Guertin M

更新日期：2006-07-25 00:00:00

abstract：:Although plasma membrane (PM) cholesterol-rich and -poor domains have been isolated by subcellular fractionation, the real-time arrangement of cholesterol in such domains in living cells is still unclear. Therefore, dehydroergosterol (DHE), a naturally occurring fluorescent sterol, was incorporated into cultured L-cel...

journal_title：Biochemistry

authors： Zhang W,McIntosh AL,Xu H,Wu D,Gruninger T,Atshaves B,Liu JC,Schroeder F

更新日期：2005-03-01 00:00:00

abstract：:The E2 protein of papillomavirus is the key regulator of viral transcription and replication. Dimerization, which takes place via its conserved C-terminal DNA-binding domain (DBD), is critical for these functions. The presence and conservation of two histidines (H290 and H320) at or near the dimer interface suggests t...

journal_title：Biochemistry

authors： Bose K,Yoder NC,Kumar K,Baleja JD

更新日期：2007-02-06 00:00:00

abstract：:ORF56 is a small and thermodynamically extremely stable dimeric protein from the archaeon Sulfolobus islandicus. This DNA binding protein is encoded on plasmid pRN1 and possibly controls the copy number of the plasmid. We report the solution NMR structure as well as the crystal structure of ORF56 comprising a ribbon-h...

journal_title：Biochemistry

authors： Weininger U,Zeeb M,Neumann P,Löw C,Stubbs MT,Lipps G,Balbach J

更新日期：2009-10-27 00:00:00

abstract：:To investigate cytosine protonation and its influence on the sequence-dependent thermal stability of DNA triplexes in detail, we have employed homo- and heteronuclear NMR experiments on specifically (15)N-labeled oligodeoxynucleotides that were designed to fold into intramolecular triple helices of the pyrimidine moti...

journal_title：Biochemistry

authors： Leitner D,Schröder W,Weisz K

更新日期：2000-05-16 00:00:00

abstract：:Terpene synthases are potentially useful biocatalysts for the synthesis of valuable compounds, such as anticancer drugs and antibiotics. The design of altered activities requires better knowledge of their mechanisms, for example, an understanding of the complex conformational changes that are part of their catalytic c...

journal_title：Biochemistry

authors： van der Kamp MW,Sirirak J,Żurek J,Allemann RK,Mulholland AJ

更新日期：2013-11-12 00:00:00

abstract：:This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...

journal_title：Biochemistry

authors： Gross A,Hubbell WL

更新日期：2002-01-29 00:00:00

abstract：:The enthalpies of transfer of hen egg white lysozyme from water to aqueous solutions of 1-propanol were determined by isothermal flow calorimetry at 10, 17, 25, and 40 degrees C in 0.04 M, pH 2 glycine buffer. Alcohol concentrations up to 3.4 M were employed. Four regions in the dependence of the enthalpy of transfer ...

journal_title：Biochemistry

authors： Sturtevant JM,Velicelebi G

更新日期：1981-05-26 00:00:00

abstract：:Exons 1 and 2 and exon 8 of the mu class GSH transferases from rat encode sequence-variable regions 1 and 4 of mu class isoenzymes, respectively. These two of four variable regions are located at the N- and C-termini of this isoenzyme class and impinge on the active site. In order to assess the influence of these vari...

journal_title：Biochemistry

authors： Zhang P,Liu S,Shan SO,Ji X,Gilliland GL,Armstrong RN

更新日期：1992-10-27 00:00:00

abstract：:Reduced intracellular drug accumulation due to the activity of the drug efflux pump ABC (B1) is a major mechanism in the resistance of cancer cells to chemotherapy. ABC (B1) is a poly specific transporter, and the molecular mechanism of its complex translocation process remains to be elucidated. To understand the proc...

journal_title：Biochemistry

authors： Storm J,Modok S,O'Mara ML,Tieleman DP,Kerr ID,Callaghan R

更新日期：2008-03-25 00:00:00

abstract：:Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acy...

journal_title：Biochemistry

authors： Ford DA,Horner CC,Gross RW

更新日期：1998-08-25 00:00:00

abstract：:Virulent strains of Clostridium sordellii cause gangrenous myonecrosis in humans. The released lethal toxin (TcsL) and hemorrhagic toxin (TcsH) are regarded as the major virulence factors. TcsL inactivates low molecular weight GTP-binding proteins of the Rho/Ras subfamilies by monoglucosylation. In cultured cell lines...

journal_title：Biochemistry

authors： Schulz F,Just I,Genth H

更新日期：2009-09-29 00:00:00

abstract：:The requirement of cytochrome c-550 (PSII-V) in photosystem II (PSII) has been assessed in Synechocystis sp. PCC 6803 containing mutations between Gly-351 and Thr-436 of the loop E domain of the chlorophyll a-binding protein CP47. Six photoautotrophic strains were utilized to compare the effect of removal of either th...

journal_title：Biochemistry

authors： Morgan TR,Shand JA,Clarke SM,Eaton-Rye JJ

更新日期：1998-10-13 00:00:00