Mechanistic diversity in a metalloenzyme superfamily.


:It is now appreciated that the relationships of proteins, particularly enzymes, within a protein superfamily can be understood not only in terms of their sequence similarities and three-dimensional structures but also by chemical threads that relate their functional attributes. The mechanistic ties among superfamily members can often be traced to a common transition state for the rate-limiting step of the reactions being catalyzed. This paper presents an analysis of a metalloenzyme superfamily, the members of which catalyze a very diverse set of reactions with unrelated transition states but a more general common mechanistic imperative. The vicinal oxygen chelate (VOC) superfamily is composed of structurally related proteins with paired beta alpha beta beta beta motifs that provide a metal coordination environment with two or three open or readily accessible coordination sites to promote direct electrophilic participation of the metal ion in catalysis. The known types of reactions that are catalyzed include isomerizations (glyoxalase I), epimerizations (methylmalonyl-CoA epimerase), oxidative cleavage of C-C bonds (extradiol dioxygenase), and nucleophilic substitutions (fosfomycin resistance proteins). The remarkable access to mechanism space that is provided by the VOC superfamily appears to derive from a simple, pseudosymmetric structural fold that maximizes the catalytic versatility of the metal center.






Armstrong RN




Has Abstract


2000-11-14 00:00:00














  • Conversion of arginine to lysine at position 70 of human dihydrofolate reductase: generation of a methotrexate-insensitive mutant enzyme.

    abstract::Arginine-70 of human dihydrofolate reductase (hDHFR) is a highly conserved residue which X-ray crystallographic data have shown to interact with the alpha-carboxylate of the terminal L-glutamate moiety of either folic acid or methotrexate (MTX). The rationale for this study was to introduce a conservative amino acid r...


    pub_type: 杂志文章


    authors: Thompson PD,Freisheim JH

    更新日期:1991-08-20 00:00:00

  • Structure and function of PA4872 from Pseudomonas aeruginosa, a novel class of oxaloacetate decarboxylase from the PEP mutase/isocitrate lyase superfamily.

    abstract::Pseudomonas aeruginosa PA4872 was identified by sequence analysis as a structurally and functionally novel member of the PEP mutase/isocitrate lyase superfamily and therefore targeted for investigation. Substrate screens ruled out overlap with known catalytic functions of superfamily members. The crystal structure of ...


    pub_type: 杂志文章


    authors: Narayanan BC,Niu W,Han Y,Zou J,Mariano PS,Dunaway-Mariano D,Herzberg O

    更新日期:2008-01-08 00:00:00

  • Fluorescence-detected circular dichroism of ethidium in vivo and bound to deoxyribonucleic acid in vitro.

    abstract::Fluorescence-detected circular dichroism (FDCD) spectra are reported for ethidium in Escherichia coli cells and bound to E. coli DNA in vitro. FDCD bands are observed at 325 and 385 nm. These bands change amplitude as the ethidium to DNA ratio changes. Spectra are similar for in vivo and in vitro measurements. However...


    pub_type: 杂志文章


    authors: Lamos ML,Turner DH

    更新日期:1985-06-04 00:00:00

  • Intermediates in excision repair by human cells: use of S1 nuclease and benzoylated naphthoylated cellulose to reveal single-strand breaks.

    abstract::Treatment of human lymphoid cells with methyl methanesulfonate (MMS) results in discontinuities in cellular DNA. Discontinuities can be detected by sedimentation through neutral sucrose gradients after digestion with S1 nuclease on top of the gradient and by benzoylated naphthoylated diethylaminoethylcellulose (BND-ce...


    pub_type: 杂志文章


    authors: Karran P,Higgins NP,Strauss B

    更新日期:1977-10-04 00:00:00

  • Three-dimensional structure of murine anti-p-azophenylarsonate Fab 36-71. 2. Structural basis of hapten binding and idiotypy.

    abstract::Comparison between the structures and solvent-accessible surfaces of the antigen-binding fragments of two murine anti-p-azophenylarsonate monoclonal antibodies, one bearing a major cross-reactive idiotype of A/J strain mice (36-71) and one lacking the idiotype (R19.9; Lascombe et al., 1989), highlight the structural b...


    pub_type: 杂志文章


    authors: Strong RK,Petsko GA,Sharon J,Margolies MN

    更新日期:1991-04-16 00:00:00

  • Molecular organization in striated domains induced by transmembrane alpha-helical peptides in dipalmitoyl phosphatidylcholine bilayers.

    abstract::Transmembrane (TM) alpha-helical peptides with neutral flanking residues such as tryptophan form highly ordered striated domains when incorporated in gel-state 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) bilayers and inspected by atomic force microscopy (AFM) (1). In this study, we analyze the molecular organiz...


    pub_type: 杂志文章


    authors: Sparr E,Ganchev DN,Snel MM,Ridder AN,Kroon-Batenburg LM,Chupin V,Rijkers DT,Killian JA,de Kruijff B

    更新日期:2005-01-11 00:00:00

  • Mapping the microtubule binding regions of calponin.

    abstract::The smooth muscle basic calponin interacts with F-actin and inhibits the actomyosin ATPase in a calmodulin or phosphorylation modulated manner. It also binds in vitro to microtubules and its acidic isoform, present in nonmuscle cells, and co-localizes with microfilaments and microtubules in cultured neurons. To assess...


    pub_type: 杂志文章


    authors: Fattoum A,Roustan C,Smyczynski C,Der Terrossian E,Kassab R

    更新日期:2003-02-11 00:00:00

  • FTIR spectroscopic characterization of the cytochrome aa3 from Acidianus ambivalens: evidence for the involvement of acidic residues in redox coupled proton translocation.

    abstract::The aa(3)-type quinol oxidase from Acidianus ambivalens is a divergent member of the heme-copper oxidases superfamily, namely, concerning the putative channels for intraprotein proton conduction. In this study, we used electrochemically induced FTIR difference spectroscopy to identify residues involved in redox-couple...


    pub_type: 杂志文章


    authors: Hellwig P,Gomes CM,Teixeira M

    更新日期:2003-05-27 00:00:00

  • Protein kinase C acylation by palmitoyl coenzyme A facilitates its translocation to membranes.

    abstract::Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acy...


    pub_type: 杂志文章


    authors: Ford DA,Horner CC,Gross RW

    更新日期:1998-08-25 00:00:00

  • Examining Reaction Specificity in PvcB, a Source of Diversity in Isonitrile-Containing Natural Products.

    abstract::Many bacteria produce isonitrile-containing natural products that are derived from aromatic amino acids. The synthetic clusters that control biosynthesis most commonly encode two enzymes, designated PvcA and PvcB, as well as additional enzymes that direct synthesis of the natural product. The PvcA enzyme installs the ...


    pub_type: 杂志文章


    authors: Zhu J,Lippa GM,Gulick AM,Tipton PA

    更新日期:2015-04-28 00:00:00

  • Extensive segregation of acidic phospholipids in membranes induced by protein kinase C and related proteins.

    abstract::Protein kinase C and two other proteins with molecular masses of 64 and 32 kDa, purified from bovine brain, constitute a type of protein that binds a large number of calcium ions in a phospholipid-dependent manner. This study suggested that these proteins also induced extensive clustering of acidic phospholipids in th...


    pub_type: 杂志文章


    authors: Bazzi MD,Nelsestuen GL

    更新日期:1991-08-13 00:00:00

  • Conformation of immunoglobulin M. 2. Nanosecond fluorescence depolarization analysis of segmental flexibility in anti-epsilon-l-dimethylamino-5-naphthalenesulfonyl-L-lysine anti-immunoglobulin from horse, pig, and shark.

    abstract::The rotational motions of immunoglobulin M (IgM) were investigated by the nanosecond fluorescence depolarization technique. The fluorophore epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine (DNS-lysine) was specifically bound in the combining sites of anti-DNS IgM antibodies from the horse, pig, and nurse shark. ...


    pub_type: 杂志文章


    authors: Holowka DA,Cathou RE

    更新日期:1976-07-27 00:00:00

  • Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site.

    abstract::Cyclopropane fatty acid (CFA) synthase of Escherichia coli catalyzes a modification of the acyl chains of phospholipid bilayers. We report (i) identification of the CFA synthase protein, (ii) overproduction (> 600-fold) and purification to essential homogeneity of the enzyme, and (iii) the amino acid sequence of CFA s...


    pub_type: 杂志文章


    authors: Wang AY,Grogan DW,Cronan JE Jr

    更新日期:1992-11-17 00:00:00

  • Selective inactivation of parvulin-like peptidyl-prolyl cis/trans isomerases by juglone.

    abstract::In contrast to FK506 binding proteins and cyclophilins, the parvulin family of peptidyl-prolyl cis/trans isomerases (PPIases; E.C. cannot be inhibited by either FK506 or cyclosporin A. We have found that juglone, 5-hydroxy-1,4-naphthoquinone, irreversibly inhibits the enzymatic activity of several parvulins, ...


    pub_type: 杂志文章


    authors: Hennig L,Christner C,Kipping M,Schelbert B,Rücknagel KP,Grabley S,Küllertz G,Fischer G

    更新日期:1998-04-28 00:00:00

  • Mapping the G-actin binding surface of cofilin using synchrotron protein footprinting.

    abstract::Cofilin is an actin regulatory protein that binds to both monomeric and filamentous actin, and has filament severing activity. Although crystal structures for the monomeric forms of both G-actin and cofilin have been described, the structure of the binary cofilin-G-actin complex is not available. Synchrotron protein f...


    pub_type: 杂志文章


    authors: Guan JQ,Vorobiev S,Almo SC,Chance MR

    更新日期:2002-05-07 00:00:00

  • Picosecond resolution of tyrosine fluorescence and anisotropy decays by 2-GHz frequency-domain fluorometry.

    abstract::We extended the technique of frequency-domain fluorometry to an upper frequency limit of 2000 MHz. This was accomplished by using the harmonic content of a laser pulse train (3.76 MHz, 5 ps) from a synchronously pumped and cavity-dumped dye laser. We used a microchannel plate photomultiplier as the detector to obtain ...


    pub_type: 杂志文章


    authors: Lakowicz JR,Laczko G,Gryczynski I

    更新日期:1987-01-13 00:00:00

  • Membrane-induced conformational change of alpha1-acid glycoprotein characterized by vacuum-ultraviolet circular dichroism spectroscopy.

    abstract::The tertiary structure of alpha1-acid glycoprotein (AGP) remains unresolved despite its novel function because AGP is a hard target in X-ray and NMR analyses. To elucidate the membrane-induced conformational change of AGP, the vacuum-ultraviolet circular dichroism (VUVCD) spectra of AGP and its constituent sugars were...


    pub_type: 杂志文章


    authors: Matsuo K,Namatame H,Taniguchi M,Gekko K

    更新日期:2009-09-29 00:00:00

  • Calitoxin, a neurotoxic peptide from the sea anemone Calliactis parasitica: amino acid sequence and electrophysiological properties.

    abstract::We have isolated a new toxin, calitoxin (CLX), from the sea anemone Calliactis parasitica whose amino acid sequence differs greatly from that of other sea anemone toxins. The polypeptide chain contains 46 amino acid residues, with a molecular mass of 4886 Da and an isoelectric point at pH 5.4. The amino acid sequence ...


    pub_type: 杂志文章


    authors: Cariello L,de Santis A,Fiore F,Piccoli R,Spagnuolo A,Zanetti L,Parente A

    更新日期:1989-03-21 00:00:00

  • Studies with recombinant Saccharomyces cerevisiae CaaX prenyl protease Rce1p.

    abstract::Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...


    pub_type: 杂志文章


    authors: Dolence JM,Steward LE,Dolence EK,Wong DH,Poulter CD

    更新日期:2000-04-11 00:00:00

  • The H187R mutation of the human prion protein induces conversion of recombinant prion protein to the PrP(Sc)-like form.

    abstract::Prion diseases are associated with a conformational switch in the prion protein (PrP) from its normal cellular form (denoted PrP(C)) to a disease-associated "scrapie" form (PrP(Sc)). A number of PrP(Sc)-like conformations can be generated by incubating recombinant PrP(C) at low pH, indicating that protonation of key r...


    pub_type: 杂志文章


    authors: Hosszu LL,Tattum MH,Jones S,Trevitt CR,Wells MA,Waltho JP,Collinge J,Jackson GS,Clarke AR

    更新日期:2010-10-12 00:00:00

  • Chemical basis for the selectivity of the von Hippel Lindau tumor suppressor pVHL for prolyl-hydroxylated HIF-1alpha.

    abstract::In animals, the post-translational hydroxylation of hypoxia inducible factor (HIF) is a central mechanism for regulating gene expression in an oxygen-dependent manner. The oxygenase-catalyzed trans-4-prolyl hydroxylation of HIF-alpha increases its affinity for the von Hippel Lindau protein elongin B/C (VCB) ubiquitin ...


    pub_type: 杂志文章


    authors: Illingworth CJ,Loenarz C,Schofield CJ,Domene C

    更新日期:2010-08-17 00:00:00

  • Recognition of G-U mismatches by tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III).

    abstract::The coordination complex tris(4,7-diphenyl-1,10-phenanthroline)rhodium(III) [Rh(DIP)3(3+)], which promotes RNA cleavage upon photoactivation, has been shown to target specifically guanine-uracil (G-U) mismatches in double-helical regions of folded RNAs. Photoactivated cleavage by Rh(DIP)3(3+) has been examined on a se...


    pub_type: 杂志文章


    authors: Chow CS,Barton JK

    更新日期:1992-06-23 00:00:00

  • Mechanism of proton transfer in the isomerization of 5-androstene-3, 17-dione by 3-oxo-delta 5-steroid isomerase and its D38E mutant.

    abstract::The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...


    pub_type: 杂志文章


    authors: Zawrotny ME,Hawkinson DC,Blotny G,Pollack RM

    更新日期:1996-05-21 00:00:00

  • Metalation and Maturation of Zinc Ectoenzymes: A Perspective.

    abstract::Numerous zinc ectoenzymes are folded and activated in the compartments of the early secretory pathway, such as the ER and the Golgi apparatus, before reaching their final destination. During this process, zinc must be incorporated into the active site; therefore, metalation of the nascent protein is indispensable for ...


    pub_type: 杂志文章


    authors: Kambe T

    更新日期:2020-01-14 00:00:00

  • Regulatory mechanisms of tumor suppressor P16(INK4A) and their relevance to cancer.

    abstract::P16(INK4A) (also known as P16 and MTS1), a protein consisting exclusively of four ankyrin repeats, is recognized as a tumor suppressor mainly because of the prevalence of genetic inactivation of the p16(INK4A) (or CDKN2A) gene in virtually all types of human cancers. However, it has also been shown that an elevated le...


    pub_type: 杂志文章,评审


    authors: Li J,Poi MJ,Tsai MD

    更新日期:2011-06-28 00:00:00

  • Mechanism of irreversible inhibition of O2 evolution in photosystem II by Tris(hydroxymethyl)aminomethane.

    abstract::The dark reaction of tris(hydroxymethyl)aminomethane (Tris) with the O2-evolving center of photosystem II (PSII) in the S1 state causes irreversible inhibition of O2 evolution. Similar inhibition is observed for several other amines: NH3, CH3NH2, (CH3)2NH, ethanolamine, and 2-amino-2-ethyl-1,3-propanediol. In PSII mem...


    pub_type: 杂志文章


    authors: Rickert KW,Sears J,Beck WF,Brudvig GW

    更新日期:1991-08-13 00:00:00

  • Site-directed mutagenesis of highly conserved residues in helix VIII of subunit I of the cytochrome bo ubiquinol oxidase from Escherichia coli: an amphipathic transmembrane helix that may be important in conveying protons to the binuclear center.

    abstract::Cytochrome bo from Escherichia coli is a ubiquinol oxidase which is a member of the superfamily of heme-copper respiratory oxidases. This superfamily, which includes the eukaryotic cytochrome c oxidases, has in common a bimetallic center consisting of a high-spin heme component and a copper atom (CuB) which is the sit...


    pub_type: 杂志文章


    authors: Thomas JW,Lemieux LJ,Alben JO,Gennis RB

    更新日期:1993-10-19 00:00:00

  • Catecholamine-stimulated guanosine 5'-O-(3-thiotriphosphate) binding to the stimulatory GTP-binding protein of adenylate cyclase: kinetic analysis in reconstituted phospholipid vesicles.

    abstract::The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with re...


    pub_type: 杂志文章


    authors: Asano T,Ross EM

    更新日期:1984-11-06 00:00:00

  • Analysis of the ternary interaction of the red cell membrane skeletal proteins spectrin, actin, and 4.1.

    abstract::Spectrin dimers interact weakly with F-actin under physiological solvent conditions (with an association constant of about 5 X 10(3) M-1 at 20 degrees C). In the presence of the membrane skeletal constituent, protein 4.1, strong binding is observed; an analysis of the profiles for formation of a ternary complex leads ...


    pub_type: 杂志文章


    authors: Ohanian V,Wolfe LC,John KM,Pinder JC,Lux SE,Gratzer WB

    更新日期:1984-09-11 00:00:00

  • Characterization of a transient intermediate formed in the liver alcohol dehydrogenase catalyzed reduction of 3-hydroxy-4-nitrobenzaldehyde.

    abstract::The compounds 3-hydroxy-4-nitrobenzaldehyde and 3-hydroxy-4-nitrobenzyl alcohol are introduced as new chromophoric substrates for probing the catalytic mechanism of horse liver alcohol dehydrogenase (LADH). Ionization of the phenolic hydroxyl group shifts the spectrum of the aldehyde from 360 to 433 nm (pKa = 6.0), wh...


    pub_type: 杂志文章


    authors: MacGibbon AK,Koerber SC,Pease K,Dunn MF

    更新日期:1987-06-02 00:00:00