Protein kinase C acylation by palmitoyl coenzyme A facilitates its translocation to membranes.

Abstract:

:Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acylation with palmitoyl CoA that facilitates its interaction with membrane bilayers. First, incubation of purified rabbit brain PKC with [14C]palmitoyl CoA (5 microM) resulted in the radiolabeling of an 80 kDa band demonstrated by SDS-PAGE and autoradiography, while incubation of PKC with other acyl CoA molecular species (e.g., [3H]myristoyl CoA or [14C]arachidonoyl CoA), fatty acids (e.g., [14C]palmitic and [14C]arachidonic acid), or [14C]diacylglycerol did not result in the incorporation of radiolabel. Second, multiple extractions of [14C]palmitoyl CoA-treated PKC with butanol did not remove the radiolabeled moiety from the 80 kDa PKC band. Third, incubation of the [14C]palmitoyl CoA-radiolabeled PKC moiety with neutral hydroxylamine, hydrochloric acid, or sodium hydroxide released incorporated radiolabel which identified the association between PKC and palmitic acid as a covalent thioester linkage. Fourth, formation of the [14C]palmitoyl CoA-radiolabeled PKC adduct could be prevented by pretreatment of PKC with either dithiobis(nitrobenzoic acid) or N-ethylmaleimide. Fifth, limited trypsinolysis of palmitoylated PKC demonstrated that palmitic acid was exclusively present in the regulatory fragment of PKC without detectable amounts of palmitic acid associated with the catalytic fragment. Sixth, palmitoylated PKC was resolved from its nonpalmitoylated counterpart by Mono Q chromatography, and palmitoylated PKC preferentially associated with cellular membranes while nonpalmitoylated PKC did not. Both palmitoylated and nonpalmitoylated PKC were activated by phosphatidylserine, diacylglycerol, and calcium ion. Collectively, these results demonstrate the acylation of PKC by palmitoyl CoA and identify a novel mechanism which may facilitate the interaction of PKC with biologic membranes.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ford DA,Horner CC,Gross RW

doi

10.1021/bi980565w

subject

Has Abstract

pub_date

1998-08-25 00:00:00

pages

11953-61

issue

34

eissn

0006-2960

issn

1520-4995

pii

bi980565w

journal_volume

37

pub_type

杂志文章
  • Simulation-based prediction of phosphatidylinositol 4,5-bisphosphate binding to an ion channel.

    abstract::Protein-lipid interactions regulate many membrane protein functions. Using a multiscale approach that combines coarse-grained and atomistic molecular dynamics simulations, we have predicted the binding site for the anionic phospholipid phosphatidylinositol 4,5-bisphosphate (PIP(2)) on the Kir2.2 inwardly rectifying (K...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi301350s

    authors: Schmidt MR,Stansfeld PJ,Tucker SJ,Sansom MS

    更新日期:2013-01-15 00:00:00

  • Kinetics of cytochrome b oxidation in antimycin-treated submitochondrial particles.

    abstract::It has been shown that in bovine heart submitochondrial particles, antimycin and 2-heptyl-4-hydroxyquinoline N-oxide (HQNO) inhibit the oxidation of NADH, succinate, and reduced ubiquinone incompletely, the uninhibited rate being about 20-40 nmol of substrate oxidized min-1 (mg of protein)-1. By contrast, rotenone, cy...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00268a045

    authors: Hatefi Y,Yagi T

    更新日期:1982-12-07 00:00:00

  • Probing the Vibrio harveyi luciferase beta subunit functionality and the intersubunit domain by site-directed mutagenesis.

    abstract::While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00206a023

    authors: Xin X,Xi L,Tu SC

    更新日期:1994-10-11 00:00:00

  • Cysteine-rich secretory protein 3 is a ligand of alpha1B-glycoprotein in human plasma.

    abstract::Human cysteine-rich secretory protein 3 (CRISP-3; also known as SGP28) belongs to a family of closely related proteins found in mammals and reptiles. Some mammalian CRISPs are known to be involved in the process of reproduction, whereas some of the CRISPs from reptiles are neurotoxin-like substances found in lizard sa...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048823e

    authors: Udby L,Sørensen OE,Pass J,Johnsen AH,Behrendt N,Borregaard N,Kjeldsen L

    更新日期:2004-10-12 00:00:00

  • Two homologues encoding human UDP-glucose:glycoprotein glucosyltransferase differ in mRNA expression and enzymatic activity.

    abstract::UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that operates as a gatekeeper for quality control by preventing transport of improperly folded glycoproteins out of the ER. We report the isolation of two cDNAs encoding human UDP-glucose:glycoprotein glucosyltran...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9916473

    authors: Arnold SM,Fessler LI,Fessler JH,Kaufman RJ

    更新日期:2000-03-07 00:00:00

  • Structural basis for herbicidal inhibitor selectivity revealed by comparison of crystal structures of plant and mammalian 4-hydroxyphenylpyruvate dioxygenases.

    abstract::A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi049323o

    authors: Yang C,Pflugrath JW,Camper DL,Foster ML,Pernich DJ,Walsh TA

    更新日期:2004-08-17 00:00:00

  • Catalytic center of cyclodextrin glycosyltransferase derived from X-ray structure analysis combined with site-directed mutagenesis.

    abstract::An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 2.4.1.19) that had been shortly exposed to beta-cyclodextrin showed density corresponding to a maltose bound at the catalytic center. The crystal structure was refined to an R-factor of 18.7...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00152a009

    authors: Klein C,Hollender J,Bender H,Schulz GE

    更新日期:1992-09-22 00:00:00

  • Electron paramagnetic resonance imaging of rat heart with nitroxide and polynitroxyl-albumin.

    abstract::Electron paramagnetic resonance (EPR) imaging utilizing stable nitroxyl radicals is a promising technique for measuring free radical distribution, metabolism, and tissue oxygenation in organs and tissues [Kuppusamy, P., Chzhan, M., Vij, K., Shteynbuk, M., Lefer, D. J., Giannella, E., & Zweier, J. L. (1994) Proc. Natl....

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi952857s

    authors: Kuppusamy P,Wang P,Zweier JL,Krishna MC,Mitchell JB,Ma L,Trimble CE,Hsia CJ

    更新日期:1996-06-04 00:00:00

  • Shortening the HIV-1 TAR RNA Bulge by a Single Nucleotide Preserves Motional Modes over a Broad Range of Time Scales.

    abstract::Helix-junction-helix (HJH) motifs are flexible building blocks of RNA architecture that help define the orientation and dynamics of helical domains. They are also frequently involved in adaptive recognition of proteins and small molecules and in the formation of tertiary contacts. Here, we use a battery of nuclear mag...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.6b00285

    authors: Merriman DK,Xue Y,Yang S,Kimsey IJ,Shakya A,Clay M,Al-Hashimi HM

    更新日期:2016-08-16 00:00:00

  • Effects of cholesterol on surface activity and surface topography of spread surfactant films.

    abstract::Pulmonary surfactant forms a monolayer of lipids and proteins at the alveolar air/liquid interface. Although cholesterol is a natural component of surfactant, its function in surface dynamics is unclear. To further elucidate the role of cholesterol in surfactant, we used a captive bubble surfactometer (CBS) to measure...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0256532

    authors: Diemel RV,Snel MM,Van Golde LM,Putz G,Haagsman HP,Batenburg JJ

    更新日期:2002-12-17 00:00:00

  • Enthalpic and entropic effects of salt and polyol osmolytes on site-specific protein-DNA association: the integrase Tn916-DNA complex.

    abstract::The effect of low molecular-weight compounds on the equilibrium constant K(A) can be used to explore the energetics and molecular mechanism of protein-DNA interactions. Here we use the complex composed of the integrase Tn916 DNA-binding domain and its target DNA duplex to investigate the effects of salt and the nonion...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048907n

    authors: Milev S,Bosshard HR,Jelesarov I

    更新日期:2005-01-11 00:00:00

  • RNA phosphorylation: a polynucleotide kinase function in mouse L cell nuclei.

    abstract::Analysis of [gamma-32P]ATP in vitro labeled nuclear RNA shows transfer of gamma-32P from ATP to form the 5'-terminal monophosphate for large RNA molecules. This finding is an actively transcribing nuclear system capable of guanylation and methylation reactions indicates that polynucleotide kinase activity in the eukar...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00638a016

    authors: Winicov I

    更新日期:1977-09-20 00:00:00

  • The hydrogen-bonding structure in parallel-stranded duplex DNA is reverse Watson-Crick.

    abstract::Raman spectra of the parallel-stranded duplex formed from the deoxyoligonucleotides 5'-d-[(A)10TAATTTTAAATATTT]-3' (D1) and 5'-d[(T)10ATTAAAATTTATAAA]-3' (D2) in H2O and D2O have been acquired. The spectra of the parallel-stranded DNA are then compared to the spectra of the antiparallel double helix formed from the de...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00226a012

    authors: Otto C,Thomas GA,Rippe K,Jovin TM,Peticolas WL

    更新日期:1991-03-26 00:00:00

  • Probing conformational changes within LC2 domains of cardiac myosin.

    abstract::Nine monoclonal antibodies were used to test calcium and EDTA effects on the molecular conformation of ventricular VLC2 within myosin. Antibody epitopes were located in six domains of VLC2 using recombinant proteins. The apparent association constants of these antibodies were measured in solution in the presence of ca...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00208a005

    authors: Eldin P,Cathiard AM,Anoal M,Léger J,Mornet D,Léger JJ,Cornillon B

    更新日期:1994-10-25 00:00:00

  • Internal dynamics of the tryptophan repressor (TrpR) and two functionally distinct TrpR variants, L75F-TrpR and A77V-TrpR, in their l-Trp-bound forms.

    abstract::Backbone amide dynamics of the Escherichia coli tryptophan repressor protein (WT-TrpR) and two functionally distinct variants, L75F-TrpR and A77V-TrpR, in their holo (l-tryptophan corepressor-bound) form have been characterized using (15)N nuclear magnetic resonance (NMR) relaxation. The three proteins possess very si...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi200389k

    authors: Tripet BP,Goel A,Copie V

    更新日期:2011-06-14 00:00:00

  • Chemically Induced Changes to Membrane Permeability in Living Cells Probed with Nonlinear Light Scattering.

    abstract::Second-harmonic light scattering (SHS) permits characterization of membrane-specific molecular transport in living cells. Herein, we demonstrate the use of time-resolved SHS for quantifying chemically induced enhancements in membrane permeability. As proof of concept, we examine the enhanced permeability of the cytopl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.5b00600

    authors: Wilhelm MJ,Sharifian Gh M,Dai HL

    更新日期:2015-07-28 00:00:00

  • Sterol and pH interdependence in the binding, oligomerization, and pore formation of Listeriolysin O.

    abstract::Listeriolysin O (LLO) is the most important virulence factor of the intracellular pathogen Listeria monocytogenes. Its main task is to enable escape of bacteria from the phagosomal vacuole into the cytoplasm. LLO belongs to the cholesterol-dependent cytolysin (CDC) family but differs from other members, as it exhibits...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi602497g

    authors: Bavdek A,Gekara NO,Priselac D,Gutiérrez Aguirre I,Darji A,Chakraborty T,Macek P,Lakey JH,Weiss S,Anderluh G

    更新日期:2007-04-10 00:00:00

  • The flavoprotein component of the Escherichia coli sulfite reductase: expression, purification, and spectral and catalytic properties of a monomeric form containing both the flavin adenine dinucleotide and the flavin mononucleotide cofactors.

    abstract::The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E. coli and purified as a monomer. The N-terminal part of the native protein contains thus al...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9728699

    authors: Zeghouf M,Fontecave M,Macherel D,Covès J

    更新日期:1998-04-28 00:00:00

  • Delineation of the Cdc42/Rac-binding domain of p21-activated kinase.

    abstract::p21-activated kinases (PAKs) serve as effector proteins for the GTP-binding proteins Cdc42 and Rac. They are serine/threonine kinases containing the Cdc42/Rac interactive binding (CRIB) motif. The main aim of this study was to define the minimal domain of alphaPAK required for Cdc42/Rac binding. Eight stable PAK fragm...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi980140+

    authors: Thompson G,Owen D,Chalk PA,Lowe PN

    更新日期:1998-05-26 00:00:00

  • GrpE accelerates nucleotide exchange of the molecular chaperone DnaK with an associative displacement mechanism.

    abstract::The ATP hydrolysis and protein-binding and release cycle of the molecular chaperone DnaK is regulated by the accessory proteins GrpE and DnaJ. Here we describe a study of the formation of complexes between the molecular chaperone DnaK, its nucleotide exchange factor GrpE, and the fluorescent ADP analog N8-[4-[(N'-meth...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi962835l

    authors: Packschies L,Theyssen H,Buchberger A,Bukau B,Goody RS,Reinstein J

    更新日期:1997-03-25 00:00:00

  • Interaction of fusion peptides from HIV gp41 with membranes: a time-resolved membrane binding, lipid mixing, and structural study.

    abstract::The interaction of the so-called fusion peptide of the human immunodeficiency virus gp41 envelope glycoprotein with the target cell membrane is believed to trigger the fusion process which allows the entry of the virus into the cell. Many studies on the interaction of the fusion peptide with biological membranes have ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi050382r

    authors: Buzón V,Padrós E,Cladera J

    更新日期:2005-10-11 00:00:00

  • 1H NMR and NOE studies of the purple acid phosphatases from porcine uterus and bovine spleen.

    abstract::The diiron active sites of the purple acid phosphatases from porcine uterus (also called uteroferrin, Uf) and bovine spleen (BSPAP) and their complexes with tungstate are compared by 1H NMR and NOE techniques. The paramagnetically shifted features of the 1H NMR spectrum of reduced BSPAP are similar to those of reduced...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00138a004

    authors: Wang Z,Ming LJ,Que L Jr,Vincent JB,Crowder MW,Averill BA

    更新日期:1992-06-16 00:00:00

  • Dynamic coupling and allosteric behavior in a nonallosteric protein.

    abstract::Long-range intraprotein interactions give rise to many important protein behaviors. Understanding how energy is transduced through protein structures to either transmit a signal or elicit conformational changes is therefore a current challenge in structural biology. In an effort to understand such linkages, multiple V...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi060652l

    authors: Clarkson MW,Gilmore SA,Edgell MH,Lee AL

    更新日期:2006-06-27 00:00:00

  • Isotopic probes of the argininosuccinate lyase reaction.

    abstract::The mechanism of the argininosuccinate lyase reaction has been probed by the measurement of the effects of isotopic substitution at the reaction centers. A primary deuterium isotope effect of 1.0 on both V and V/K is obtained with (2S,3R)-argininosuccinate-3-d, while a primary 15N isotope effect on V/K of 0.9964 +/- 0...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00365a004

    authors: Kim SC,Raushel FM

    更新日期:1986-08-26 00:00:00

  • Effect of four-alpha-helix bundle cavity size on volatile anesthetic binding energetics.

    abstract::Currently, it is thought that inhalational anesthetics cause anesthesia by binding to ligand-gated ion channels. This is being investigated using four-alpha-helix bundles, small water-soluble analogues of the transmembrane domains of the "natural" receptor proteins. The study presented here specifically investigates h...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi034623b

    authors: Manderson GA,Michalsky SJ,Johansson JS

    更新日期:2003-09-30 00:00:00

  • Thermodynamic characterization of single mismatches found in naturally occurring RNA.

    abstract::Many naturally occurring RNA structures contain single mismatches. However, the algorithms currently used to predict RNA structure from sequence rely on a minimal set of data for single mismatches, most of which occur rather infrequently in nature. As a result, several approximations and assumptions are used to predic...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi701311c

    authors: Davis AR,Znosko BM

    更新日期:2007-11-20 00:00:00

  • Determination of the rate-limiting steps for malic enzyme by the use of isotope effects and other kinetic studies.

    abstract::Isotope effects have been measured with Mg2+ as the activator and L-malate labeled with deuterium or tritium at carbon 2 as the substrate over the pH range 4-10. Comparison of the nearly pH-independent deuterium-isotope effect on V/Kmalate of 1.5 with the tritium effect of 2.0 by the method of Northrop (Northrop, D.B....

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00623a002

    authors: Schimerlik MI,Grimshaw CE,Cleland WW

    更新日期:1977-02-22 00:00:00

  • Solute diffusion in lipid bilayer membranes: an atomic level study by molecular dynamics simulation.

    abstract::To elucidate the mechanism of solute diffusion through lipid bilayer membranes, nearly 4 ns of molecular dynamics simulations of solutes in phospholipid bilayers was conducted. The study, the first atomic level study of solute diffusion in a lipid bilayer, involved four simulations of an all-atom representation of a f...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00210a010

    authors: Bassolino-Klimas D,Alper HE,Stouch TR

    更新日期:1993-11-30 00:00:00

  • Relative thermodynamic stability of DNA, RNA, and DNA:RNA hybrid duplexes: relationship with base composition and structure.

    abstract::Fourteen oligonucleotides 8-21 nucleotides in length and their complements were synthesized as DNA and RNA. For each sequence, four kinds of duplexes, DNA:DNA, RNA:RNA, DNA:RNA, and RNA:DNA, were prepared. Twelve sequences had A.T/U content varying from 25 to 80% and dPy content in the DNA strands varying from 0 to 10...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00034a013

    authors: Lesnik EA,Freier SM

    更新日期:1995-08-29 00:00:00

  • Engineered disulfide linking the hinge regions within lactose repressor dimer increases operator affinity, decreases sequence selectivity, and alters allostery.

    abstract::The hinge domain encompasses amino acids 51-60 of lactose repressor (LacI) and plays an important role in its regulatory interaction with operator DNA. This segment makes both hinge-DNA and hinge-hinge' contacts that are critical to DNA binding. Furthermore, this small region serves as a central element in communicati...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0114067

    authors: Falcon CM,Matthews KS

    更新日期:2001-12-25 00:00:00