Protein kinase C acylation by palmitoyl coenzyme A facilitates its translocation to membranes.

Abstract:

:Protein kinase C (PKC) translocation to specific subcellular membrane loci after cellular stimulation is mediated, in part, through its interaction with diacylglycerol, phosphatidylserine, and calcium. Herein, we present multiple lines of evidence which demonstrate that purified rabbit brain PKC undergoes specific acylation with palmitoyl CoA that facilitates its interaction with membrane bilayers. First, incubation of purified rabbit brain PKC with [14C]palmitoyl CoA (5 microM) resulted in the radiolabeling of an 80 kDa band demonstrated by SDS-PAGE and autoradiography, while incubation of PKC with other acyl CoA molecular species (e.g., [3H]myristoyl CoA or [14C]arachidonoyl CoA), fatty acids (e.g., [14C]palmitic and [14C]arachidonic acid), or [14C]diacylglycerol did not result in the incorporation of radiolabel. Second, multiple extractions of [14C]palmitoyl CoA-treated PKC with butanol did not remove the radiolabeled moiety from the 80 kDa PKC band. Third, incubation of the [14C]palmitoyl CoA-radiolabeled PKC moiety with neutral hydroxylamine, hydrochloric acid, or sodium hydroxide released incorporated radiolabel which identified the association between PKC and palmitic acid as a covalent thioester linkage. Fourth, formation of the [14C]palmitoyl CoA-radiolabeled PKC adduct could be prevented by pretreatment of PKC with either dithiobis(nitrobenzoic acid) or N-ethylmaleimide. Fifth, limited trypsinolysis of palmitoylated PKC demonstrated that palmitic acid was exclusively present in the regulatory fragment of PKC without detectable amounts of palmitic acid associated with the catalytic fragment. Sixth, palmitoylated PKC was resolved from its nonpalmitoylated counterpart by Mono Q chromatography, and palmitoylated PKC preferentially associated with cellular membranes while nonpalmitoylated PKC did not. Both palmitoylated and nonpalmitoylated PKC were activated by phosphatidylserine, diacylglycerol, and calcium ion. Collectively, these results demonstrate the acylation of PKC by palmitoyl CoA and identify a novel mechanism which may facilitate the interaction of PKC with biologic membranes.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Ford DA,Horner CC,Gross RW

doi

10.1021/bi980565w

subject

Has Abstract

pub_date

1998-08-25 00:00:00

pages

11953-61

issue

34

eissn

0006-2960

issn

1520-4995

pii

bi980565w

journal_volume

37

pub_type

杂志文章
  • Chemical-enzymatic insertion of an amino acid residue in the reactive site of soybean trypsin inhibitor (Kunitz).

    abstract::Modified (Arg63-Ile64 reactive-site peptide bond hydrolyzed) soybean trypsin inhibitor (Kunitz) with all reactive amino groups, except that of Ile64, protected was described in the preceding paper (Kowalski, D., and Laskowski, M., Jr. (1976), Biochemistry, preceding paper in this issue). Treatment of this inhibitor wi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00651a022

    authors: Kowalski D,Laskowski M Jr

    更新日期:1976-03-23 00:00:00

  • Binding of cytochrome P450 monooxygenase and lipoxygenase pathway products by heart fatty acid-binding protein.

    abstract::Arachidonic acid metabolism by lipoxygenases and cytochrome P450 monooxygenases produces regioisomeric hydroperoxyeicosatetraenoic acids (HPETEs), hydroxyeicosatetraenoic acids (HETEs), epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acids (DHETs), which serve as components of cell signaling cascades. In...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi001602y

    authors: Widstrom RL,Norris AW,Spector AA

    更新日期:2001-01-30 00:00:00

  • Near-infrared resonance Raman spectra of Chloroflexus aurantiacus photosynthetic reaction centers.

    abstract::Resonance Raman spectra of the photosynthetic reaction center isolated from the green bacterium Chloroflexus aurantiacus have been obtained with excitation in the near-infrared absorption bands of the special pair (P) and the accessory bacteriochlorophyll (B) using shifted-excitation Raman difference spectroscopy (SER...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00015a044

    authors: Cherepy NJ,Holzwarth AR,Mathies RA

    更新日期:1995-04-18 00:00:00

  • Ligand binding properties of bacterial hemoglobins and flavohemoglobins.

    abstract::Bacterial hemoglobins and flavohemoglobins share a common globin fold but differ otherwise in structural and functional aspects. The bases of these differences were investigated through kinetic studies on oxygen, carbon monoxide, and nitric oxide binding. The novel bacterial hemoglobins from Clostridium perfringens an...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi047389d

    authors: Farrés J,Rechsteiner MP,Herold S,Frey AD,Kallio PT

    更新日期:2005-03-15 00:00:00

  • Neurotoxins active on insects: amino acid sequences, chemical modifications, and secondary structure estimation by circular dichroism of toxins from the scorpion Androctonus australis Hector.

    abstract::Two scorpion neurotoxins active only on insects, the insect toxins AaH IT1 and AaH IT2, were purified from the venom of scorpions Androctonus australis Hector collected in Tozeur (Tunisia) and characterized. AaH IT2 was sequenced and found to differ in four amino acid positions from AaH IT, the single previously seque...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00458a021

    authors: Loret EP,Mansuelle P,Rochat H,Granier C

    更新日期:1990-02-13 00:00:00

  • Kinetic properties of a MNB/DYRK1A mutant suitable for the elucidation of biochemical pathways.

    abstract::Minibrain kinase/dual-specificity tyrosine phosphorylation regulated kinase 1A (MNB/DYRK1A) is a proline/arginine-directed serine/threonine kinase implicated in the learning deficits of Down syndrome. Epigallocatechin-3-gallate (EGCG), the major tea polyphenolic compound, is a potent MNB/DYRK1A inhibitor. In this stud...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi060632j

    authors: Adayev T,Chen-Hwang MC,Murakami N,Wegiel J,Hwang YW

    更新日期:2006-10-03 00:00:00

  • Deep-UV resonance Raman analysis of the Rhodobacter capsulatus cytochrome bc₁complex reveals a potential marker for the transmembrane peptide backbone.

    abstract::Classical strategies for structure analysis of proteins interacting with a lipid phase typically correlate ensemble secondary structure content measurements with changes in the spectroscopic responses of localized aromatic residues or reporter molecules to map regional solvent environments. Deep-UV resonance Raman (DU...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi200596w

    authors: Halsey CM,Oshokoya OO,Jiji RD,Cooley JW

    更新日期:2011-08-02 00:00:00

  • Proposed mechanism for the condensation reaction of citrate synthase: 1.9-A structure of the ternary complex with oxaloacetate and carboxymethyl coenzyme A.

    abstract::The crystal structure of the ternary complex citrate synthase-oxaloacetate-carboxymethyl coenzyme A has been solved to a resolution of 1.9 A and refined to a conventional crystallographic R factor of 0.185. The structure resembles a proposed transition state of the condensation reaction and suggests that the condensat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:

    authors: Karpusas M,Branchaud B,Remington SJ

    更新日期:1990-03-06 00:00:00

  • Direct determination of the association constant between elongation factor Tu X GTP and aminoacyl-tRNA using fluorescence.

    abstract::We have investigated the formation of the aa-tRNA X EF-Tu X GTP ternary complex spectroscopically by monitoring a fluorescence change that accompanies the association of EF-Tu X GTP with Phe-tRNAPhe-F8, a functionally active analogue of Phe-tRNAPhe with a fluorescein moiety covalently attached to the s4U-8 base. With ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00324a023

    authors: Abrahamson JK,Laue TM,Miller DL,Johnson AE

    更新日期:1985-01-29 00:00:00

  • Cooperative binding of polyamines induces the Escherichia coli single-strand binding protein-DNA binding mode transitions.

    abstract::The Escherichia coli single-strand binding (SSB) protein is an essential protein involved in DNA replication, recombination, and repair processes. The tetrameric protein binds to ss nucleic acids in a number of different binding modes in vitro. These modes differ in the number of nucleotides occluded per SSB tetramer ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00141a029

    authors: Wei TF,Bujalowski W,Lohman TM

    更新日期:1992-07-07 00:00:00

  • Interaction of alamethicin with lecithin bilayers: a 31P and 2H NMR study.

    abstract::The interaction of alamethicin with artificial lecithin multilamellar dispersions was investigated by nuclear magnetic resonance (NMR) and Raman spectroscopies. 31P NMR studies revealed perturbation of the lipid head groups in the presence of the icosapeptide. Simulation of the 31P NMR spectra indicated that the obser...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00347a019

    authors: Banerjee U,Zidovetzki R,Birge RR,Chan SI

    更新日期:1985-12-17 00:00:00

  • Engineering of fibroblast growth factor: alteration of receptor binding specificity.

    abstract::A five-residue loop structure in basic fibroblast growth factor (FGF-2) which extends from amino acid residue 118 to residue 122 was replaced, by cassette mutagenesis, with the corresponding seven-residue loop structure from the structural homologue acidic fibroblast growth factor (FGF-1) or the corresponding five-res...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00003a004

    authors: Seddon AP,Aviezer D,Li LY,Böhlen P,Yayon A

    更新日期:1995-01-24 00:00:00

  • Reconstitution and further characterization of the cholesterol transport activity of the small-intestinal brush border membrane.

    abstract::The sterol (free and esterified cholesterol) transport activity of the small-intestinal brush border membrane was solubilized with the short-chain detergent diheptanoylphosphatidylcholine and reconstituted to an artificial membrane system (proteoliposomes). The resulting proteoliposomes were identified as unilamellar ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970625i

    authors: Boffelli D,Weber FE,Compassi S,Werder M,Schulthess G,Hauser H

    更新日期:1997-09-02 00:00:00

  • Insulin signaling in the yeast Saccharomyces cerevisiae. 1. Stimulation of glucose metabolism and Snf1 kinase by human insulin.

    abstract::Effects of human insulin on glucose metabolism in the yeast Saccharomyces cerevisiae were studied in this report. Under two conditions of growth limitation (glucose-grown cells during transition to stationary phase or spheroplasts during incubation in synthetic glucose medium), human insulin (10 and 1 microM, respecti...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi972071p

    authors: Müller G,Rouveyre N,Crecelius A,Bandlow W

    更新日期:1998-06-16 00:00:00

  • Sulfated glycoproteins, glycolipids, and glycosaminoglycans from synaptic plasma and myelin membranes: isolation and characterization of sulfated glycopeptides.

    abstract::In this report we provide biochemical evidence that a highly purified synaptic plasma membrane fraction derived from rat brain, after intraventricular injection of 35S-labeled sodium sulfate, is enriched in a number of large sulfated glycoproteins compared with a purified myelin fraction studied concurrently. A fracti...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00670a005

    authors: Simpson DL,Thorne DR,Loch HH

    更新日期:1976-12-14 00:00:00

  • Leaving group dependence and proton inventory studies of the phosphorylation of a cytoplasmic phosphotyrosyl protein phosphatase from bovine heart.

    abstract::The kcat and Km values for the bovine heart low molecular weight phosphotyrosyl protein phosphatase catalyzed hydrolysis of 16 aryl phosphate monoesters and of five alkyl phosphate monoesters having the structure Ar(CH2)nOPO3H2 (n = 1-5) were measured at pH 5.0 and 37 degrees C. With the exception of alpha-naphthyl ph...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00101a006

    authors: Zhang ZY,Van Etten RL

    更新日期:1991-09-17 00:00:00

  • Effect of sequence on the conformation of DNA holliday junctions.

    abstract::Structures of the DNA sequences d(CCGGCGCCGG) and d(CCAGTACbr(5)UGG) are presented here as four-way Holliday junctions in their compact stacked-X forms, with antiparallel alignment of the DNA strands. Thus, the ACC-trinucleotide motif, previously identified as important for stabilizing the junction, is now extended to...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0346603

    authors: Hays FA,Vargason JM,Ho PS

    更新日期:2003-08-19 00:00:00

  • Induced alpha-helix structure in the aryl hydrocarbon receptor transactivation domain modulates protein-protein interactions.

    abstract::The aryl hydrocarbon receptor (AhR) is an intracellular receptor protein that regulates gene transcription in response to both man-made and natural ligands. A modular transactivaton domain (TAD) has been mapped to the 304 C-terminal amino acids and consists of acidic, Q-rich, and P/S/T-rich subdomains. We have used st...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0487701

    authors: Watt K,Jess TJ,Kelly SM,Price NC,McEwan IJ

    更新日期:2005-01-18 00:00:00

  • Isotope-edited Fourier transform infrared spectroscopy studies of calmodulin's interaction with its target peptides.

    abstract::The ubiquitous calcium-binding protein calmodulin (CaM) regulates a wide variety of cellular events by binding to and activating many distinct target enzymes. The CaM-binding domains of most of these enzymes are contained in a contiguous stretch of amino acids with a length of approximately 20 residues. In this work, ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00202a006

    authors: Zhang M,Fabian H,Mantsch HH,Vogel HJ

    更新日期:1994-09-13 00:00:00

  • Effect of reversed heme orientation on circular dichroism and cooperative oxygen binding of human adult hemoglobin.

    abstract::We found that recombinant human adult hemoglobin (rHb A) expressed in Escherichia coli showed heterogeneity of components with the intensity of a positive CD band at 260 nm and that it could be resolved into three components (SP-1, SP-2, and SP-3) by SP-Sepharose column chromatography. 1H NMR revealed that SP-1 is ide...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi7015519

    authors: Nagai M,Nagai Y,Aki Y,Imai K,Wada Y,Nagatomo S,Yamamoto Y

    更新日期:2008-01-15 00:00:00

  • Molecular functions of conserved aspects of the GHMP kinase family.

    abstract::The sequences and three-dimensional structures of the galactokinase, homoserine kinase, mevalonate kinase, and phosphomevalonate kinase (GHMP) family were compared to identify highly conserved surface residues. The functions of these solvent-accessible residues were assessed by determining the effects of their substit...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048963o

    authors: Andreassi JL 2nd,Leyh TS

    更新日期:2004-11-23 00:00:00

  • Excitation-Wavelength-Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila.

    abstract::Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b01114

    authors: Mix LT,Carroll EC,Morozov D,Pan J,Gordon WR,Philip A,Fuzell J,Kumauchi M,van Stokkum I,Groenhof G,Hoff WD,Larsen DS

    更新日期:2018-03-20 00:00:00

  • Clustering and dynamics of phototransducer signaling domains revealed by site-directed spin labeling electron paramagnetic resonance on SRII/HtrII in membranes and nanodiscs.

    abstract::In halophilic archaea the photophobic response is mediated by the membrane-embedded 2:2 photoreceptor/-transducer complex SRII/HtrII, the latter being homologous to the bacterial chemoreceptors. Both systems bias the rotation direction of the flagellar motor via a two-component system coupled to an extended cytoplasmi...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi501160q

    authors: Orban-Glaß I,Voskoboynikova N,Busch KB,Klose D,Rickert C,Mosslehy W,Roder F,Wilkens V,Piehler J,Engelhard M,Steinhoff HJ,Klare JP

    更新日期:2015-01-20 00:00:00

  • Mutational analysis of the roles in catalysis and substrate recognition of arginines 54 and 305, aspartic acid 309, and tryptophan 317 located at subsites 1 and 2 in glucoamylase from Aspergillus niger.

    abstract::The mutants Arg54-->Leu, Arg54-->Lys, Arg305-->Lys, Asp309-->Glu, and Trp317-->Phe, located at subsites 1 and 2 in glucoamylase from Aspergillus niger, provide insight into the importance of specific hydrogen bonds and hydrophobic interactions in substrate recognition, catalytic mechanism, and stability. As suggested ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00032a009

    authors: Frandsen TP,Christensen T,Stoffer B,Lehmbeck J,Dupont C,Honzatko RB,Svensson B

    更新日期:1995-08-15 00:00:00

  • Peptide cross-linking to calmodulin: attachment of [Tyr8]substance P.

    abstract::Ultraviolet irradiation of calmodulin in the presence of calcium results in either the intramolecular cross-linking of Tyr99 and Tyr138 [Malencik, D.A., & Anderson, S.R. (1986) Biochemistry 25, 709] or, when [Tyr8]substance P is bound, the generation of peptide-calmodulin adducts. The latter consist of two chromatogra...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00403a016

    authors: Malencik DA,Anderson SR

    更新日期:1988-02-09 00:00:00

  • Steady-state and transient kinetic analyses of taurine/alpha-ketoglutarate dioxygenase: effects of oxygen concentration, alternative sulfonates, and active-site variants on the FeIV-oxo intermediate.

    abstract::Taurine/alpha-ketoglutarate (alphaKG) dioxygenase (TauD), an archetype alphaKG-dependent hydroxylase, is a non-heme mononuclear Fe(II) enzyme that couples the oxidative decarboxylation of alphaKG with the conversion of taurine to aminoacetaldehyde and sulfite. The crystal structure of taurine-alphaKG-Fe(II)TauD is kno...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi048746n

    authors: Grzyska PK,Ryle MJ,Monterosso GR,Liu J,Ballou DP,Hausinger RP

    更新日期:2005-03-15 00:00:00

  • Conformational change coupling the dimerization and activation of KSHV protease.

    abstract::The mechanism of herpesviral protease activation upon dimerization was studied using two independent spectroscopic assays augmented by directed mutagenesis. Spectroscopic changes, attributable to dimer interface conformational plasticity, were observed upon dimerization of Kaposi's sarcoma-associated herpesvirus prote...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi011753g

    authors: Pray TR,Reiling KK,Demirjian BG,Craik CS

    更新日期:2002-02-05 00:00:00

  • Inhibition mechanism of rat α₃β₄ nicotinic acetylcholine receptor by the Alzheimer therapeutic tacrine.

    abstract::Nicotinic acetylcholine receptors (nAChRs) were studied in detail in the past regarding their interaction with therapeutic and drug addiction related compounds. Using fast kinetic whole-cell recording, we have now studied effects of tacrine, an agent used clinically to treat Alzheimer's disease, on currents elicited b...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi101789y

    authors: Cheffer A,Ulrich H

    更新日期:2011-03-22 00:00:00

  • An experimental approach to evaluating the role of backbone interactions in proteins using unnatural amino acid mutagenesis.

    abstract::The contribution of backbone hydrogen bonds in alpha-helices to the overall stability of a protein has been examined experimentally by replacing several backbone amide linkages in alpha-helix 39-50 of T4 lysozyme with ester linkages. T4 lysozyme variants wherein the backbone amide bonds between residues Ser38 and Leu3...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9707685

    authors: Koh JT,Cornish VW,Schultz PG

    更新日期:1997-09-23 00:00:00

  • The 2.15 A crystal structure of Mycobacterium tuberculosis chorismate mutase reveals an unexpected gene duplication and suggests a role in host-pathogen interactions.

    abstract::Chorismate mutase catalyzes the first committed step toward the biosynthesis of the aromatic amino acids, phenylalanine and tyrosine. While this biosynthetic pathway exists exclusively in the cell cytoplasm, the Mycobacterium tuberculosis enzyme has been shown to be secreted into the extracellular medium. The secretor...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0606445

    authors: Qamra R,Prakash P,Aruna B,Hasnain SE,Mande SC

    更新日期:2006-06-13 00:00:00