Abstract:
:The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosX(Ml)) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activity toward 1 and does not confer resistance to the antibiotic. In vitro homologous recombination of the mlr3345 and pa1129 genes (a fosA gene from Pseudomonas aeruginosa that does confer robust resistance to 1) produces recombinant proteins that confer resistance to 1 and indicate that the FosA resistance proteins are functionally and genetically related to mlr3345.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Brown DW,Schaab MR,Birmingham WR,Armstrong RNdoi
10.1021/bi900078qsubject
Has Abstractpub_date
2009-03-10 00:00:00pages
1847-9issue
9eissn
0006-2960issn
1520-4995pii
10.1021/bi900078qjournal_volume
48pub_type
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