Evolution of the antibiotic resistance protein, FosA, is linked to a catalytically promiscuous progenitor.

Abstract:

:The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosX(Ml)) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activity toward 1 and does not confer resistance to the antibiotic. In vitro homologous recombination of the mlr3345 and pa1129 genes (a fosA gene from Pseudomonas aeruginosa that does confer robust resistance to 1) produces recombinant proteins that confer resistance to 1 and indicate that the FosA resistance proteins are functionally and genetically related to mlr3345.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Brown DW,Schaab MR,Birmingham WR,Armstrong RN

doi

10.1021/bi900078q

subject

Has Abstract

pub_date

2009-03-10 00:00:00

pages

1847-9

issue

9

eissn

0006-2960

issn

1520-4995

pii

10.1021/bi900078q

journal_volume

48

pub_type

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