当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Lipoprotein lipase catalyzed hydrolysis of water-soluble p-nitrophenyl esters. Inhibition by apolipoprotein C-II.

Lipoprotein lipase catalyzed hydrolysis of water-soluble p-nitrophenyl esters. Inhibition by apolipoprotein C-II.

Abstract：

:Bovine milk lipoprotein lipase (LpL) catalyzes the hydrolysis of the water-soluble esters p-nitrophenyl acetate (PNPA) and p-nitrophenyl butyrate (PNPB). The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol hydrolysis and PNPB hydrolysis activities coelute from the heparin-Sepharose affinity column used to purify LpL and (b) LpL-catalyzed hydrolyses of trioleoylglycerol and PNPB are inhibited to equal extents by phenylmethanesulfonyl fluoride. The effect of apolipoprotein C-II (apoC-II) on the LpL-catalyzed hydrolysis of PNPA and PNPB has been determined. ApoC-II inhibits hydrolysis of both esters, with a maximum extent of inhibition of 70-90%. Inhibition of the LpL-catalyzed hydrolysis of PNPB is specific for apoC-II, since apolipoproteins A-I, C-I, and C-III-2 have little effect on this reaction, and is partial noncompetitive in form. KI values for apoC-II inhibition of the LpL-catalyzed hydrolysis of PNPA and PNPB are in the range 0.26-0.83 microM. The effect of apoC-II on the temperature dependences of LpL-catalyzed hydrolysis of both esters and on NaCl inhibition of LpL-catalyzed PNPB hydrolysis is consistent with a change in rate-determining step with LpL and apoC-II interact. These results indicate not only that there is an interaction between apoC-II and LpL in aqueous solution in the absence of a lipid interface but also that this interaction conformationally modulates the active site of the enzyme.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Quinn DM,Shirai K,Jackson RL,Harmony JA

doi

10.1021/bi00269a038

subject

Has Abstract

pub_date

1982-12-21 00:00:00

pages

6872-9

issue

26

eissn

0006-2960

issn

1520-4995

journal_volume

21

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Methyl-esterified proteins in a mammalian cell line.

    abstract：:Methyl esterification of carboxylic acid residues in intact mouse S49 lymphoma cells was examined, and at least 24 proteins were found to be modified. Cell fractionation revealed that a distinct set of these proteins could be found in each of the four fractions. Nuclei contained 11 methyl-esterified proteins at 12, 15...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00344a053

    authors： Chelsky D,Ruskin B,Koshland DE Jr

    更新日期：1985-11-05 00:00:00

  • Effect of ATP analogues on the actin-myosin interface.

    abstract：:The interaction between skeletal myosin subfragment 1 (S1) and filamentous actin was examined at various intermediate states of the actomyosin ATPase cycle by chemical cross-linking experiments. Reaction of the actin-S1 complex with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide and N-hydroxysuccinimide generated pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi980139a

    authors： Van Dijk J,Fernandez C,Chaussepied P

    更新日期：1998-06-09 00:00:00

  • Acid- and calcium-induced structural changes in phosphatidylethanolamine membranes stabilized by cholesteryl hemisuccinate.

    abstract：:The membrane stabilization effect of cholesteryl hemisuccinate (CHEMS) and the sensitivity of the CHEMS-phosphatidylethanolamine membranes to protons and calcium ions were studied by differential scanning calorimetry, freeze-fracture electron microscopy, and 31P NMR. (1) At neutral pH, the addition of 8 mol % CHEMS to...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00328a013

    authors： Lai MZ,Vail WJ,Szoka FC

    更新日期：1985-03-26 00:00:00

  • Dynamic nature of the quaternary structure of the vesicular stomatitis virus envelope glycoprotein.

    abstract：:The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00462a002

    authors： Lyles DS,Varela VA,Parce JW

    更新日期：1990-03-13 00:00:00

  • Rational engineering of mannosyl binding in the distal glycone subsites of Cellulomonas fimi endo-beta-1,4-mannanase: mannosyl binding promoted at subsite -2 and demoted at subsite -3.

    abstract：:To date, rational redesign of glycosidase active-site clefts has been mainly limited to the removal of essential functionalities rather than their introduction. The glycoside hydrolase family 26 endo-beta-1,4-mannanase from the soil bacterium Cellulomonas fimi depolymerizes various abundant plant mannans. On the basis...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100097f

    authors： Hekmat O,Lo Leggio L,Rosengren A,Kamarauskaite J,Kolenova K,Stålbrand H

    更新日期：2010-06-15 00:00:00

  • Metal-ligand vibrations of cyanoferric myeloperoxidase and cyanoferric horseradish peroxidase: evidence for a constrained heme pocket in myeloperoxidase.

    abstract：:The low-frequency FeCN vibrations of cyanoferric myeloperoxidase (MPO) and horseradish peroxidase (HRP) have been measured by resonance Raman spectroscopy. The ordering of the frequencies of the predominantly FeC stretching and FeCN bending normal vibrational modes in the two peroxidases differs. These normal mode vib...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00492a012

    authors： López-Garriga JJ,Oertling WA,Kean RT,Hoogland H,Wever R,Babcock GT

    更新日期：1990-10-09 00:00:00

  • Chemistry Is Dead. Long Live Chemistry!

    abstract：:Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the "photon budget" require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, pr...

    journal_title：Biochemistry

    pub_type： 杂志文章,评审

    doi：10.1021/acs.biochem.7b00529

    authors： Lavis LD

    更新日期：2017-10-03 00:00:00

  • Oxygen binding constants for human hemoglobin tetramers.

    abstract：:High-precision studies of oxygen binding in hemoglobin (HbA0) solutions at near-physiological concentrations (2-12 mM heme; pHs 7.0-9.1; various buffers) have led to an unanticipated result: an unmeasurably low contribution from the triply ligated species. We have obtained this result from new differential oxygen-bind...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00387a038

    authors： Gill SJ,Di Cera E,Doyle ML,Bishop GA,Robert CH

    更新日期：1987-06-30 00:00:00

  • Interruption of the internal water chain of cytochrome f impairs photosynthetic function.

    abstract：:The structure of cytochrome f includes an internal chain of five water molecules and six hydrogen-bonding side chains, which are conserved throughout the phylogenetic range of photosynthetic organisms from higher plants, algae, and cyanobacteria. The in vivo electron transfer capability of Chlamydomonas reinhardtii cy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0004596

    authors： Sainz G,Carrell CJ,Ponamarev MV,Soriano GM,Cramer WA,Smith JL

    更新日期：2000-08-08 00:00:00

  • Altering the Enantioselectivity of Tyrosyl-tRNA Synthetase by Insertion of a Stereospecific Editing Domain.

    abstract：:Translation of mRNAs by the ribosome is stereospecific, with only l-amino acids being incorporated into the nascent polypeptide chain. This stereospecificity results from the exclusion of d-amino acids at three steps during protein synthesis: (1) the aminoacylation of tRNA by aminoacyl-tRNA synthetases, (2) binding of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01167

    authors： Richardson CJ,First EA

    更新日期：2016-03-15 00:00:00

  • Distribution of shape-changing compounds across the red cell membrane.

    abstract：:The effects of two oppositely charged pyrene derivatives, 1-pyrenebutyrylcholine (PBC) and 1-pyrenebutyric acid (PBA), on red blood cell shape have been examined. Both compounds convert normal biconcave erythrocytes into echinocytes. However, with extended incubation time at elevated temperature, the morphology of PBC...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00556a002

    authors： Matayoshi ED

    更新日期：1980-07-22 00:00:00

  • Probing receptor binding activity of interleukin-8 dimer using a disulfide trap.

    abstract：:Interleukin-8 (IL-8), a member of the chemokine superfamily, exists as both monomers and dimers, and mediates its function by binding to neutrophil CXCR1 and CXCR2 receptors that belong to the G protein-coupled receptor class. It is now well established that the monomer functions as a high-affinity ligand, but the bin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0605944

    authors： Rajarathnam K,Prado GN,Fernando H,Clark-Lewis I,Navarro J

    更新日期：2006-06-27 00:00:00

  • The possible relationship between a membrane conformational change and photosystem II dependent hydrogen ion accumulation and adenosine 5'-triphosphate synthesis.

    abstract：:Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfo...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00691a008

    authors： Giaquinta RT,Ort DR,Dilley RA

    更新日期：1975-10-07 00:00:00

  • Gene heterogeneity: a basis for alternative 5.8S rRNA processing.

    abstract：:Two bands of 5.8S rRNA were observed when the total RNA isolated from rat or mouse tissue was separated by electrophoresis on high-resolution polyacrylamide gels under denaturing conditions. The minor form, with a lower mobility, represented 15-35% of the total 5.8S rRNA, depending on the source of the tissue. Sequenc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00311a011

    authors： Smith SD,Banerjee N,Sitz TO

    更新日期：1984-07-31 00:00:00

  • Evidence for a tRNA/rRNA interaction site within the peptidyltransferase center of the Escherichia coli ribosome.

    abstract：:A nine-base oligodeoxyribonucleotide complementary to bases 2497-2505 of 23S rRNA was hybridized to both 50S subunits and 70S ribosomes. The binding of the probe to the ribosome or ribosomal subunits was assayed by nitrocellulose filtration and by sucrose gradient centrifugation techniques. The location of the hybridi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00428a073

    authors： Marconi RT,Hill WE

    更新日期：1989-01-24 00:00:00

  • Formation of a bis(histidyl) heme iron complex in manganese peroxidase at high pH and restoration of the native enzyme structure by calcium.

    abstract：:Manganese peroxidase (MnP) from Phanerochaete chrysosporium undergoes a pH-dependent conformational change evidenced by changes in the electronic absorption spectrum. This high- to low-spin alkaline transition occurs at approximately 2 pH units lower in an F190I mutant MnP when compared to the wild-type enzyme. Herein...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000679j

    authors： Youngs HL,Moënne-Loccoz P,Loehr TM,Gold MH

    更新日期：2000-08-15 00:00:00

  • Characterization of a recombinant extracellular domain of the type 1 tumor necrosis factor receptor: evidence for tumor necrosis factor-alpha induced receptor aggregation.

    abstract：:An expression plasmid encoding the extracellular portion of the human tumor necrosis factor (TNF) type 1 receptor (TNF-R1) was constructed and used to generate a stable cell line secreting soluble TNF-R1 (sTNF-R1). The sTNF-R1 was purified, and its biochemical properties and its interactions with human TNF-alpha were ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00119a023

    authors： Pennica D,Kohr WJ,Fendly BM,Shire SJ,Raab HE,Borchardt PE,Lewis M,Goeddel DV

    更新日期：1992-02-04 00:00:00

  • Positive and negative hepatic regulation of the human type II phospholipase A2 gene.

    abstract：:To identify the elements which regulate the liver transcription of the human type II phospholipase A2 gene and its stimulation by interleukin 6, the 5' flanking region from -1614 to +806 and several 3' and 5' deleted fragments have been analyzed in CAT assays. Negative regulatory elements have been located in the regi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00189a017

    authors： Olivier JL,Fan Q,Salvat C,Ziari M,Kong L,Mangeney M,Bereziat G

    更新日期：1994-06-14 00:00:00

  • "Nonclassical" secretion of annexin A2 to the lumenal side of the enterocyte brush border membrane.

    abstract：:Annexin A2 is a member of the annexin family of Ca(2+)-dependent lipid binding proteins and believed to be engaged in membrane transport processes in a number of cell types. In small intestinal enterocytes, we localized annexin A2 to the brush border region, where it was found mainly on the lumenal side of the microvi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0355239

    authors： Danielsen EM,van Deurs B,Hansen GH

    更新日期：2003-12-16 00:00:00

  • Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase.

    abstract：:Acetohydroxyacid synthase (AHAS, EC 4.1.3.18) catalyzes the first step in the biosynthesis of the branched-chain amino acids. In bacteria, the enzyme has a large subunit containing the catalytic machinery and a small subunit with a regulatory role. In eucaryotes, the evidence for a regulatory subunit is largely indire...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi983013m

    authors： Pang SS,Duggleby RG

    更新日期：1999-04-20 00:00:00

  • Effect of amphotericin B on membranes: a spin probe study.

    abstract：:The effect of the polyene antibiotic amphotericin B on the electron paramagnetic resonance spectra of lipid probes intercalated in model membranes was examined. When the antibiotic was added to the aqueous phase, no spectral effects occurred. However, when the antibiotic was incorporated during membrane preparation, c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00523a010

    authors： Aracava Y,Smith IC,Schreier S

    更新日期：1981-09-29 00:00:00

  • The fungal product terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA) .

    abstract：:Terreic acid is a metabolite with antibiotic properties produced by the fungus Aspergillus terreus. We found that terreic acid is a covalent inhibitor of the bacterial cell wall biosynthetic enzyme MurA from Enterobacter cloacae and Escherichia coli in vitro. The crystal structure of the MurA dead-end complex with ter...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100365b

    authors： Han H,Yang Y,Olesen SH,Becker A,Betzi S,Schönbrunn E

    更新日期：2010-05-18 00:00:00

  • Structures of enterococcal glycerol kinase in the absence and presence of glycerol: correlation of conformation to substrate binding and a mechanism of activation by phosphorylation.

    abstract：:The first structure of a glycerol kinase from a Gram-positive organism, Enterococcus casseliflavus, has been determined to 2.8 A resolution in the presence of glycerol and to 2.5 A resolution in the absence of substrate. The substrate-induced closure of 7 degrees is significantly smaller than that reported for hexokin...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi034258o

    authors： Yeh JI,Charrier V,Paulo J,Hou L,Darbon E,Claiborne A,Hol WG,Deutscher J

    更新日期：2004-01-20 00:00:00

  • Extensive segregation of acidic phospholipids in membranes induced by protein kinase C and related proteins.

    abstract：:Protein kinase C and two other proteins with molecular masses of 64 and 32 kDa, purified from bovine brain, constitute a type of protein that binds a large number of calcium ions in a phospholipid-dependent manner. This study suggested that these proteins also induced extensive clustering of acidic phospholipids in th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00246a013

    authors： Bazzi MD,Nelsestuen GL

    更新日期：1991-08-13 00:00:00

  • Dihydroorotase from the hyperthermophile Aquifex aeolicus is activated by stoichiometric association with aspartate transcarbamoylase and forms a one-pot reactor for pyrimidine biosynthesis.

    abstract：:In prokaryotes, the first three enzymes in pyrimidine biosynthesis, carbamoyl phosphate synthetase (CPS), aspartate transcarbamoylase (ATC), and dihydroorotase (DHO), are commonly expressed separately and either function independently (Escherichia coli) or associate into multifunctional complexes (Aquifex aeolicus). I...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801831r

    authors： Zhang P,Martin PD,Purcarea C,Vaishnav A,Brunzelle JS,Fernando R,Guy-Evans HI,Evans DR,Edwards BF

    更新日期：2009-02-03 00:00:00

  • 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine, a novel irreversible inhibitor of S-adenosylhomocysteine hydrolase.

    abstract：:The acetylenic analogue of adenosine 9-(5',6'-dideoxy-beta-D-ribo-hex-5'-ynofuranosyl)adenine has been synthesized, and its behavior as an inhibitor of bovine S-adenosylhomocysteine hydrolase has been examined. Incubation of the enzyme with excess inhibitor caused a time-dependent, irreversible inactivation of the enz...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00105a025

    authors： Parry RJ,Muscate A,Askonas LJ

    更新日期：1991-10-15 00:00:00

  • Conformational consequences of ionization of Lys, Asp, and Glu buried at position 66 in staphylococcal nuclease.

    abstract：:The pK(a) values measured previously for the internal Lys-66, Asp-66, and Glu-66 in variants of a highly stable form of staphylococcal nuclease are shifted by as many as 5 pK(a) units relative to normal pK(a) values in water. These shifts cannot be reproduced with continuum electrostatics calculations with static stru...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902114m

    authors： Karp DA,Stahley MR,García-Moreno B

    更新日期：2010-05-18 00:00:00

  • N-ethylmaleimide inhibits Ncd motor function by modification of a cysteine in the stalk domain.

    abstract：:N-Ethylmaleimide (NEM), which reacts readily with exposed sulfhydryl groups, has been shown to inhibit the activity of the microtubule (MT) motors kinesin, Ncd, and dynein. Currently, the mechanism of inhibition is not known for any of these proteins. To investigate the mechanism by which NEM inhibits Ncd, the recombi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi990706+

    authors： Phelps KK,Walker RA

    更新日期：1999-08-17 00:00:00

  • Multidomain initiation factor 2 from Thermus thermophilus consists of the individual autonomous domains.

    abstract：:The three-dimensional chalice-like crystal structure of initiation factor 2 IF2/eIF5B from Methanobacterium thermoautotrophicum represents a novel fold and domain architecture in which the N-terminal G domain and the C-terminal C domain are separated by an approximately 40 A alpha-helix. Homologous Thermus thermophilu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi702295g

    authors： Zoldák G,Sedlák E,Wolfrum A,Musatov A,Fedunová D,Szkaradkiewicz K,Sprinzl M

    更新日期：2008-04-29 00:00:00

  • Large-scale purification and characterization of a highly active four-subunit cytochrome bc1 complex from Rhodobacter sphaeroides.

    abstract：:A highly active, large-scale preparation of ubiquinol:cytochrome c2 oxidoreductase (EC 1.10.2.2; cytochrome bc1 complex) has been obtained from Rhodobacter sphaeroides. The enzyme was solubilized from chromatophores by using dodecyl maltoside in the presence of glycerol and was purified by anion-exchange and gel filtr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00463a004

    authors： Andrews KM,Crofts AR,Gennis RB

    更新日期：1990-03-20 00:00:00