Demonstration of the GC-rich common arm in yeast ribosomal 5.8S RNA via 500-MHz proton nuclear magnetic resonance and Overhauser enhancements.

abstract：:The flavoenzyme tryptophan 2-monooxygenase catalyzes the oxidation of tryptophan to indoleacetamide, carbon dioxide, and water. The enzyme is a homologue of l-amino acid oxidase. In the structure of l-amino acid oxidase complexed with aminobenzoate, Tyr372 hydrogen bonds with the carboxylate of the inhibitor in the ac...

journal_title：Biochemistry

authors： Sobrado P,Fitzpatrick PF

更新日期：2003-12-02 00:00:00

abstract：:We have previously suggested that folding of the SV40 attenuator RNA 1 into two hairpin elements leads to a block of transcription elongation. Using site-directed mutagenesis, we created three templates that either strengthened or weakened the proposed hairpin structures. The mutated and wild-type templates were clone...

journal_title：Biochemistry

authors： Goldring NB,Kessler M,Aloni Y

更新日期：1992-09-08 00:00:00

abstract：:Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8) are cell surface nucleotidases with two transmembranous domains, one each at the N- and C-termini. Hydrolysis of substrates occurs in active sites residing in their extracellular domains. Human ecto-ATPase activity is decreased by NP...

journal_title：Biochemistry

authors： Mukasa T,Lee Y,Knowles AF

更新日期：2005-08-23 00:00:00

abstract：:Single-molecule imaging of the entrance of a protein into the hydrophobic environment of a cell membrane was investigated. The pre-stem of LukF, one of the two components of the pore-forming toxin staphylococcal gamma-hemolysin, was specifically labeled with 6-bromoacetyl-2-dimethylaminonaphthalene (Badan), an environ...

journal_title：Biochemistry

authors： Nguyen AH,Nguyen VT,Kamio Y,Higuchi H

更新日期：2006-02-28 00:00:00

abstract：:Two classes of bacteriophages, the single-stranded DNA Microviridae and the single-stranded RNA Alloleviviridae, accomplish lysis by expressing "protein antibiotics", or polypeptides that inhibit cell wall biosynthesis. Previously, we have provided genetic and physiological evidence that E, a 91-amino acid membrane pr...

journal_title：Biochemistry

authors： Zheng Y,Struck DK,Young R

更新日期：2009-06-09 00:00:00

abstract：:Electron paramagnetic resonance (EPR) measurements were performed on photosystem II (PSII) membranes that were treated with 2 M NaCl to release the 17- and 23-kilodalton (kDa) polypeptides. By using 75 microM 3-(3,4-dichlorophenyl)-1,1-dimethylurea to limit the photosystem II samples to one stable charge separation in...

journal_title：Biochemistry

authors： de Paula JC,Li PM,Miller AF,Wu BW,Brudvig GW

更新日期：1986-10-21 00:00:00

abstract：:Slow conformational changes have been proposed to be responsible for the kinetic positive cooperativity of glucokinase (GK) with glucose. Induced-fit and preexisting equilibrium kinetic models have been previously suggested. In the present study, equilibrium and pre-steady-state fluorescence spectroscopy has been used...

journal_title：Biochemistry

authors： Antoine M,Boutin JA,Ferry G

更新日期：2009-06-16 00:00:00

abstract：:In the processes of DNA replication, recombination, and repair, duplex DNA can transiently form branched structures, such as Holliday junctions, as well as base pair mismatches and bulges. These stages have altered ligand and protein binding properties from normal double helical DNA. A variety of ligands have been rep...

journal_title：Biochemistry

authors： Hernández LI,Zhong M,Courtney SH,Marky LA,Kallenbach NR

更新日期：1994-11-08 00:00:00

abstract：:The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolut...

journal_title：Biochemistry

authors： Brisson JR,Carver JP

更新日期：1983-07-19 00:00:00

abstract：:Reactivation of tetrameric porcine skeletal muscle lactic dehydrogenase after dissociation and extensive unfolding of the monomers by 6 M guanidine hydrochloride (Gdn . HCl) is characterized by sigmoidal kinetics, indicating a complex mechanism involving rate-limiting folding and association steps. For analysis of the...

journal_title：Biochemistry

authors： Zettlmeissl G,Rudolph R,Jaenicke R

更新日期：1982-08-17 00:00:00

abstract：:The O-linked protein glycosylation pathway in Neisseria gonorrhoeae is responsible for the synthesis of a complex oligosaccharide on undecaprenyl diphosphate and subsequent en bloc transfer of the glycan to serine residues of select periplasmic proteins. Protein glycosylation (pgl) genes have been annotated on the bas...

journal_title：Biochemistry

authors： Hartley MD,Morrison MJ,Aas FE,Børud B,Koomey M,Imperiali B

更新日期：2011-06-07 00:00:00

abstract：:The metalloenzyme Cu/Zn-superoxide dismutase (SOD1) catalyzes the reduction of superoxide anions into molecular oxygen and hydrogen peroxide. Hydrogen peroxide can oxidize SOD1, resulting in aberrant protein conformational changes, disruption of SOD1 function, and DNA damage. Cells may have evolved mechanisms of regul...

journal_title：Biochemistry

authors： Auclair JR,Brodkin HR,D'Aquino JA,Petsko GA,Ringe D,Agar JN

更新日期：2013-09-10 00:00:00

abstract：:Protein serine/threonine phosphatase 2A (PP2A) is a critical regulator of numerous cellular signaling processes and a potential target for reactive electrophiles that dysregulate phosphorylation-dependent signal transduction cascades. The predominant cellular form of PP2A is a heterotrimeric holoenzyme consisting of a...

journal_title：Biochemistry

authors： Codreanu SG,Adams DG,Dawson ES,Wadzinski BE,Liebler DC

更新日期：2006-08-22 00:00:00

abstract：:Typical of many transcriptional regulatory proteins, the lambdoid bacteriophage repressors bind cooperatively to multiple sites on DNA. This cooperative binding is essential for establishment and maintenance of phage lysogeny. In the phage, two repressor homodimers, one bound at each of the adjacent operator sites, in...

journal_title：Biochemistry

authors： Ciubotaru M,Koudelka GB

更新日期：2003-04-15 00:00:00

abstract：:P-glycoprotein (P-gp) is the most well-known ATP-binding cassette (ABC) transporter involved in unidirectional substrate translocation across the membrane lipid bilayer, thereby causing the typical multidrug resistance (MDR) phenotype expressed in many cancers. We observed that in human CEM acute lymphoblastic leukemi...

journal_title：Biochemistry

authors： Gayet L,Dayan G,Barakat S,Labialle S,Michaud M,Cogne S,Mazane A,Coleman AW,Rigal D,Baggetto LG

更新日期：2005-03-22 00:00:00

abstract：:The conformational change of the model peptide Ac-K-G-R-G-D-G-amide induced by a phospholipidic interface was investigated by proton nuclear magnetic resonance (1H NMR). In aqueous solution, the free peptide is highly flexible and disordered, even in the presence of deuterated dodecyl-phosphocholine (DPC-d38) micelles...

journal_title：Biochemistry

authors： Macquaire F,Baleux F,Giaccobi E,Huynh-Dinh T,Neumann JM,Sanson A

更新日期：1992-03-10 00:00:00

abstract：:The envelope glycoprotein of HIV-1 consists of the surface subunit gp120 and the transmembrane subunit gp41. Binding of gp120 to target cell receptors induces a conformational change in gp41, which then mediates the fusion of viral and cellular membranes. A buried isoleucine (Ile573) in a central trimeric coiled coil ...

journal_title：Biochemistry

authors： Liu J,Shu W,Fagan MB,Nunberg JH,Lu M

更新日期：2001-03-06 00:00:00

abstract：:Deuterium quadrupole echo spectra and spin-lattice relaxation rates measured at 76.8 and 38.4 MHz as a function of relative humidity are reported for calf thymus DNA deuterated at positions A8 and G8. The amplitude of base pair motion is observed to increase slightly with increasing degree of hydration (up to approxim...

journal_title：Biochemistry

authors： Brandes R,Vold RR,Vold RL,Kearns DR

更新日期：1986-11-18 00:00:00

abstract：:We describe a novel, potent peptide substrate mimetic inhibitor of protein kinase B (PKB/Akt). The compound selectively kills prostate cancer cells, in which PKB is highly activated, but not normal cells, or cancer cells in which PKB is not activated. The inhibitor induces apoptosis and inhibits the phosphorylation of...

journal_title：Biochemistry

authors： Litman P,Ohne O,Ben-Yaakov S,Shemesh-Darvish L,Yechezkel T,Salitra Y,Rubnov S,Cohen I,Senderowitz H,Kidron D,Livnah O,Levitzki A,Livnah N

更新日期：2007-04-24 00:00:00

abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

journal_title：Biochemistry

authors： Laughrea M,Tam J

更新日期：1991-12-03 00:00:00

abstract：:The spin equilibria of several derivatives of human methemoglobin were probed by resonance Raman scattering. The intensity of lines in the Raman spectrum gives a measure of the high-spin (S = 5/2) to low-spin (S = 1/2) ratio which agrees well with the spin equilibria determined from direct magnetic susceptibility meas...

journal_title：Biochemistry

authors： Noble RW,DeYoung A,Rousseau DL

更新日期：1989-06-13 00:00:00

abstract：:Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...

journal_title：Biochemistry

authors： Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

更新日期：1999-03-02 00:00:00

abstract：:Fluoroaluminate and fluoroberyllate are potent inhibitors of the ATPase activity of myosin. Inhibition requires the presence of ADP, and much evidence has accumulated to suggest that the tetrahedral fluoroaluminate and fluoroberyllate ions act as phosphate analogues, binding with high affinity at the active site in th...

journal_title：Biochemistry

authors： Henry GD,Maruta S,Ikebe M,Sykes BD

更新日期：1993-10-05 00:00:00

abstract：:The molecular chaperone SecB binds to hydrophobic sections of unfolded secretory proteins and thereby prevents their premature folding prior to secretion by the translocase of Escherichia coli. Here, we have investigated the effect of the single-residue mutation of leucine 42 to arginine (L42R) centrally positioned in...

journal_title：Biochemistry

authors： Bechtluft P,Kedrov A,Slotboom DJ,Nouwen N,Tans SJ,Driessen AJ

更新日期：2010-03-23 00:00:00

abstract：:HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...

journal_title：Biochemistry

authors： Velten M,Villoutreix BO,Ladjimi MM

更新日期：2000-01-18 00:00:00

abstract：:Methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) catalyzes interconversion of 5,10-methylene-tetrahydrofolate and 10-formyl-tetrahydrofolate in the one-carbon metabolic pathway. In some organisms, the essential requirement of 10-formyl-tetrahydrofolate may also be fulfilled by formyltetrahydrofolate synth...

journal_title：Biochemistry

authors： Sah S,Varshney U

更新日期：2015-06-09 00:00:00

abstract：:Nitric oxide generated by bacterial nitric oxide synthase (NOS) increases the susceptibility of Gram-positive pathogens Staphylococcus aureus and Bacillus anthracis to oxidative stress, including antibiotic-induced oxidative stress. Not surprisingly, NOS inhibitors also improve the effectiveness of antimicrobials. Dev...

journal_title：Biochemistry

authors： Holden JK,Dejam D,Lewis MC,Huang H,Kang S,Jing Q,Xue F,Silverman RB,Poulos TL

更新日期：2015-07-07 00:00:00

abstract：:The fluorescence of the base analogue 2-aminopurine (2AP) was used to probe bacteriophage T4 DNA polymerase-induced conformational changes in the template strand produced during the nucleotide incorporation and proofreading reactions. 2AP fluorescence in DNA is quenched by 2AP interactions with neighboring bases, but ...

journal_title：Biochemistry

authors： Hariharan C,Reha-Krantz LJ

更新日期：2005-12-06 00:00:00

abstract：:Helicobacter pylori NikR (HpNikR) is a nickel-responsive transcription factor. In addition to a role in nickel homeostasis, HpNikR is proposed to serve as a master activator-repressor for H. pylori acid adaptation by directly or indirectly regulating the expression of a battery of genes. One potential mechanism of thi...

journal_title：Biochemistry

authors： Li Y,Zamble DB

更新日期：2009-03-24 00:00:00

abstract：:Thrombospondin-1 (TSP1) binding to calreticulin (CRT) on the cell surface signals focal adhesion disassembly, leading to the intermediate adhesive phenotype, cell migration, anoikis resistance, and collagen stimulation. Residues Lys 24 and 32 in TSP1 and amino acids 24-26 and 32-34 in CRT have been shown through bioch...

journal_title：Biochemistry

authors： Yan Q,Murphy-Ullrich JE,Song Y

更新日期：2011-02-01 00:00:00