Tight hydrophobic contacts with the SecB chaperone prevent folding of substrate proteins.

abstract：:Structural and dynamic properties of the self-complementary decadeoxyribonucleotide d(CGCAATTGCG)2 and the interaction between a prototype lexitropsin, or information-reading oligopeptide, and the decadeoxyribonucleotide are deduced by using high-resolution 1H NMR techniques. The nonexchangeable and imino proton reson...

journal_title：Biochemistry

authors： Lee M,Chang DK,Hartley JA,Pon RT,Krowicki K,Lown JW

更新日期：1988-01-12 00:00:00

abstract：:The EphA2 receptor plays key roles in many physiological and pathological events, including cancer. The process of receptor endocytosis and the consequent degradation have attracted attention as possible means of overcoming the negative outcomes of EphA2 in cancer cells and decreasing tumor malignancy. A recent study ...

journal_title：Biochemistry

authors： Mercurio FA,Marasco D,Pirone L,Pedone EM,Pellecchia M,Leone M

更新日期：2012-03-13 00:00:00

abstract：:The synthesis of sphingomyelin was studied in baby hamster kidney cells and in subcellular fractions derived from rat liver. During pulse-chase experiments with [3H]choline in tissue culture cells, the specific radioactivity of sphingomyelin continued to increase after the specific activities of phosphocholine and cyt...

journal_title：Biochemistry

authors： Voelker DR,Kennedy EP

更新日期：1982-05-25 00:00:00

abstract：:The fosfomycin (1) resistance proteins FosA and FosX in pathogenic microorganisms are related to a catalytically promiscuous progenitor encoded in a phn operon in Mesorhizobium loti. The mlr3345 gene product (FosX(Ml)) from M. loti has a very low epoxide hydrolase activity and even lower glutathione transferase activi...

journal_title：Biochemistry

authors： Brown DW,Schaab MR,Birmingham WR,Armstrong RN

更新日期：2009-03-10 00:00:00

abstract：:Resonance Raman (RR) spectra of soluble guanylate cyclase (sGC) reported by five independent research groups have been classified as two types: sGC(1) and sGC(2). Here we demonstrate that the RR spectra of sGC isolated from bovine lung contain only sGC(2) while both species are observed in the spectra of the CO-bound ...

journal_title：Biochemistry

authors： Li Z,Pal B,Takenaka S,Tsuyama S,Kitagawa T

更新日期：2005-01-25 00:00:00

abstract：:Incubation of 3-(2-phenylethyl)-4-methylsydnone (PMS) with liver microsomes from phenobarbital-pretreated rats or with reconstituted cytochrome P-450b results in loss of the enzyme chromophore. Chromophore loss is NADPH-dependent even though the sydnone decomposes by an oxygen- but not enzyme-dependent process to give...

journal_title：Biochemistry

authors： Grab LA,Swanson BA,Ortiz de Montellano PR

更新日期：1988-06-28 00:00:00

abstract：:The relaxin-like factor (RLF) also named insulin-like 3 (INSL3) consists of two polypeptide chains linked by two interchain and one intrachain disulfide bond. RLF binds to its receptor (LGR8 also named RXFP2) through the B chain and initiates transmembrane communication by activating the adenylate cyclase through the ...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：2012-05-22 00:00:00

abstract：:The mechanism of codon recognition by tRNA is investigated in the system tRNAPhe + UUC by temperature-jump measurements using the Wye base fluorescence as a label. In 0.4 M Na+ and 5 mM Mg2+ a two-step reaction is observed and described quantitatively; UUC is shown to bind preferentially to one of two conformations on...

journal_title：Biochemistry

authors： Labuda D,Pörschke D

更新日期：1980-08-05 00:00:00

abstract：:Light-driven outward sodium-pumping rhodopsin (NaR) was recently found in marine bacteria. Krokinobacter eikastus rhodopsin 2 (KR2) actively transports sodium and lithium ions in NaCl and LiCl, respectively, while it pumps protons in KCl. NaR has a conserved NDQ (N112, D116, and Q123 in KR2) motif, and previous studie...

journal_title：Biochemistry

authors： Abe-Yoshizumi R,Inoue K,Kato HE,Nureki O,Kandori H

更新日期：2016-10-18 00:00:00

abstract：:ROS-GC represents a membrane guanylate cyclase subfamily whose distinctive feature is that it transduces diverse intracellularly generated Ca(2+) signals into the production of the second messenger cyclic GMP. An intriguing feature of the first subfamily member, ROS-GC1, is that it is both stimulated and inhibited by ...

journal_title：Biochemistry

authors： Kumar VD,Vijay-Kumar S,Krishnan A,Duda T,Sharma RK

更新日期：1999-09-28 00:00:00

abstract：:Intensive study has been devoted to understanding the kinetic and structural bases underlying the exceptionally high fidelity (low error frequencies) of the typical DNA polymerase. Commonly proposed explanations have included (i) the concept of fidelity check points, in which the correctness of a nascent base pair mat...

journal_title：Biochemistry

authors： Showalter AK,Tsai MD

更新日期：2002-08-27 00:00:00

abstract：:The PLL(PTE-like lactonase)-group of enzymes within the amidohydrolase superfamily hydrolyze N-acyl-homoserine lactones (AHLs) that are involved in bacterial quorum-sensing pathways. These enzymes possess the (beta/alpha)(8)-barrel fold and serve as attractive templates for in vitro evolution and engineering of quorum...

journal_title：Biochemistry

authors： Chow JY,Wu L,Yew WS

更新日期：2009-05-26 00:00:00

abstract：:Complement components C5b-6 and C7 assemble to form C5b-7, which then interacts with membranes and commits the membrane attack complex to a target site. This protein-membrane association event was investigated to determine possible structural features that could contribute to a selective membrane attack. This system m...

journal_title：Biochemistry

authors： Silversmith RE,Nelsestuen GL

更新日期：1986-11-18 00:00:00

abstract：:Enzyme divergence within three species of the genus Photobacterium (P. fischeri, P. leiognathi, and P. phosphoreum) was studied by comparing the catalytic characteristics and quaternary interactions of bacterial luciferases isolated from each species. Each luciferase was composed of two subunits of different molecular...

journal_title：Biochemistry

authors： Ruby EG,Hastings JW

更新日期：1980-10-28 00:00:00

abstract：:alpha5beta1 integrin can occupy several distinct conformational states which support different strengths of binding to fibronectin [García, A. J., et al. (1998) J. Biol. Chem. 273, 34710-34715]. Using a model system in which specific activating monoclonal antibodies were used to achieve uniform activated states, the b...

journal_title：Biochemistry

authors： García AJ,Schwarzbauer JE,Boettiger D

更新日期：2002-07-23 00:00:00

abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

journal_title：Biochemistry

authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

更新日期：2007-06-05 00:00:00

abstract：:Inefficient maturation and trafficking to the cell surface of the cystic fibrosis transmembrane conductance regulator (CFTR) is the primary cause of cystic fibrosis. CFTR protein that fails to mature accumulates as an immature core-glycosylated protein and is rapidly degraded. To determine how the structures of mature...

journal_title：Biochemistry

authors： Chen EY,Bartlett MC,Clarke DM

更新日期：2000-04-04 00:00:00

abstract：:The structure and folding of a novel human insulin mutant, [Thr(B27) --> Pro, Pro(B28) --> Thr]insulin (PT insulin), in aqueous solution and in mixtures of water and 2,2,2-trifluoroethanol (TFE) have been studied by NMR spectroscopy. It was found that PT insulin has a highly flexible structure in pure water and is pre...

journal_title：Biochemistry

authors： Keller D,Clausen R,Josefsen K,Led JJ

更新日期：2001-09-04 00:00:00

abstract：:We investigated the molecular mechanisms and the binding site location for the fluorophor crystal violet (CrV), a noncompetitive antagonist of the nicotinic acetylcholine receptor (AChR). To this end, radiolabeled competition binding, fluorescence spectroscopy, Schild-type analysis, patch-clamp recordings, and molecul...

journal_title：Biochemistry

authors： Arias HR,Bhumireddy P,Spitzmaul G,Trudell JR,Bouzat C

更新日期：2006-02-21 00:00:00

abstract：:Guanylin, an intestinal peptide hormone and endogenous ligand of guanylyl cyclase C, is produced as the corresponding prohormone proguanylin. The mature hormone consists of 15 amino acid residues, representing the COOH-terminal part of the prohormone comprised of 94 amino acid residues. Here we report the recombinant ...

journal_title：Biochemistry

authors： Lauber T,Nourse A,Schulz A,Marx UC

更新日期：2002-12-10 00:00:00

abstract：:Fibrinogen (approximately 10(-5) M) labilizes heterologous interactions within the thrombin-modified factor XIII zymogen (i.e., XIII' = a2'b2) so that, in the time frame (ca. 10 min) of normal clotting in plasma (37 degrees C, mu = 0.15, pH 7.5), 1.5 mM Ca2+ is sufficient to cause the release of the noncatalytic b sub...

journal_title：Biochemistry

authors： Credo RB,Curtis CG,Lorand L

更新日期：1981-06-23 00:00:00

abstract：:The effects of an inhibitory monoclonal antibody (mAb) raised against the beta subunit of the Escherichia coli RNA polymerase were determined on the kinetics and structural interactions during formation of the open promoter complex (RPo). Analysis of the kinetics of abortive initiation on linear and supercoiled templa...

journal_title：Biochemistry

authors： Rockwell P,Krakow JS

更新日期：1988-05-03 00:00:00

abstract：:It had been reported previously that the beta2 subunit of Escherichia coli tryptophan synthetase [L-serinehydrolyase (adding indole) EC 4.2.1.20] can be cleaved by trypsin into a nearly functional dimeric protein, the monomer of which consists of two large, nonoverlapping, polypeptide fragments. In the present paper, ...

journal_title：Biochemistry

authors： Högberg-Raibaud A,Goldberg ME

更新日期：1977-09-06 00:00:00

abstract：:Heme uptake and utilization by pathogenic bacteria are critical for virulence and disease, since heme and heme proteins are a major source of iron within the host. Although the role of outer membrane heme receptors in this process has been extensively characterized at the genetic and biochemical level, the role of the...

journal_title：Biochemistry

authors： Kaur AP,Wilks A

更新日期：2007-03-20 00:00:00

abstract：:The stimulatory GTP-binding protein of adenylate cyclase, Gs, and beta-adrenergic receptors were reconstituted into unilamellar phospholipid vesicles. The kinetics of the quasiirreversible binding of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) to Gs, equivalent to Gs activation by nucleotide, was studied with re...

journal_title：Biochemistry

authors： Asano T,Ross EM

更新日期：1984-11-06 00:00:00

abstract：:Isolation and characterization of a novel radiation-induced product, i.e., the 8-hydroxyguanine residue, produced in deoxyribonucleic acid (DNA), 2'-deoxyguanosine, and 2'-deoxyguanosine 5'-monophosphate by gamma-irradiation in aqueous solution, are described. For this purpose, gamma-irradiated DNA was first hydrolyze...

journal_title：Biochemistry

authors： Dizdaroglu M

更新日期：1985-07-30 00:00:00

abstract：:To test the prediction that extensively fluorinated (fluorous) proteins should be more stable and exhibit novel self-segregating behavior, the properties of the de novo designed model 4-alpha-helix bundle protein, alpha 4F 6, in which the hydrophobic core is packed entirely with the extensively fluorinated amino acid ...

journal_title：Biochemistry

authors： Gottler LM,de la Salud-Bea R,Marsh EN

更新日期：2008-04-15 00:00:00

abstract：:The clathrin assembly protein complex AP-2 is a multimeric subunit complex consisting of two 100-115-kDa subunits known as alpha and beta and 50- and 16-kDa subunits. The subunits have been dissociated and separated by ion-exchange chromatography in 7.5 M urea. Fractions highly enriched in either the alpha or beta sub...

journal_title：Biochemistry

authors： Prasad K,Keen JH

更新日期：1991-06-04 00:00:00

abstract：:Mdm2 negatively regulates p53 by inhibiting its transcriptional activity and promoting its degradation by functioning as an E3 ubiquitin ligase. The primary p53 binding site on mdm2 is located in its N-terminal domain. Through binding to p53 at its N-terminal transactivation domain, mdm2 directly blocks the transcript...

journal_title：Biochemistry

authors： Ma J,Martin JD,Zhang H,Auger KR,Ho TF,Kirkpatrick RB,Grooms MH,Johanson KO,Tummino PJ,Copeland RA,Lai Z

更新日期：2006-08-01 00:00:00

abstract：:The biosynthesis of epothilones, a family of hybrid polyketide (PK)/nonribosomal peptide (NRP) antitumor agents, provides an ideal system to study a hybrid PK/NRP natural product with significant biomedical value. Here the third enzyme involved in epothilone production, the five domain 195 kDa polyketide synthase (PKS...

journal_title：Biochemistry

authors： O'Connor SE,Chen H,Walsh CT

更新日期：2002-04-30 00:00:00