Interaction of ribosomal protein S1 and initiation factor IF3 with the 3' major domain and the decoding site of the 30S subunit of Escherichia coli.

abstract：:The preferential in vitro methylation of histone H3 by coactivator-associated arginine methyltransferase 1 (CARM1) has been proposed as a basis for its ability to enhance gene transcription [Chen, D., et al. (1999) Science 284, 2174-2177]. To further evaluate the significance of H3 methylation, we studied the kinetics...

journal_title：Biochemistry

authors： Schurter BT,Koh SS,Chen D,Bunick GJ,Harp JM,Hanson BL,Henschen-Edman A,Mackay DR,Stallcup MR,Aswad DW

更新日期：2001-05-15 00:00:00

abstract：:The limited proteolysis approach was used to analyze the conformational features of human growth hormone (hGH) under acidic solvent conditions (A-state). Pepsin was used as the proteolytic probe because of its poor substrate specificity and its activity at low pH. Limited proteolysis of hGH in its A-state results in a...

journal_title：Biochemistry

authors： Spolaore B,Polverino de Laureto P,Zambonin M,Fontana A

更新日期：2004-06-01 00:00:00

abstract：:Apolipoprotein E (apoE) isoforms are key determinants of susceptibility to late-onset Alzheimer's disease (AD). The epsilon 4 and epsilon 2 alleles have been associated with increased and decreased risk for AD, respectively. We have generated and characterized transgenic mice in which the human apoE2 gene is expressed...

journal_title：Biochemistry

authors： Georgopoulos S,McKee A,Kan HY,Zannis VI

更新日期：2002-07-30 00:00:00

abstract：:Escherichia coli non-heme-binding ferritin A (EcFtnA) is a spherical cagelike protein that is composed of 24 identical subunits. EcFtnA dissociates into 2-mers under acidic conditions and can reassemble into the native structure when the pH is increased. To understand how electrostatic interactions influence the assem...

journal_title：Biochemistry

authors： Sato D,Takebe S,Kurobe A,Ohtomo H,Fujiwara K,Ikeguchi M

更新日期：2016-01-26 00:00:00

abstract：:The binding properties of substrates and competitive inhibitors of Escherichia coli cytidine deaminase are compared with those of the fragments obtained by cutting these ligands at several positions including the glycosidic bond. In contrast with the normal substrate cytidine (kcat/Km = 2.6 x 10(6) M-1 s-1), cytosine ...

journal_title：Biochemistry

authors： Carlow D,Wolfenden R

更新日期：1998-08-25 00:00:00

abstract：:The protein tyrosine kinase p72syk readily phosphorylates hematopoietic linkage cell-specific protein p50/HS1 with high stoichiometry (up to 4 mol of Pi/mol of protein) and favorable kinetic constants (Km 77 nM, kcat 0.37 s-1), at sites that display the motif that is specifically recognized by the HS2 domains of Src t...

journal_title：Biochemistry

authors： Ruzzene M,Brunati AM,Marin O,Donella-Deana A,Pinna LA

更新日期：1996-04-23 00:00:00

abstract：:Protein kinases are key regulators of diverse signaling networks critical for growth and development. Protein kinase A (PKA) is an important kinase prototype that phosphorylates protein targets at Ser and Thr residues by converting ATP to ADP. Mg(2+) ions play a crucial role in regulating phosphoryl transfer and can l...

journal_title：Biochemistry

authors： Khavrutskii IV,Grant B,Taylor SS,McCammon JA

更新日期：2009-12-08 00:00:00

abstract：:The translocation (T) domain of diphtheria toxin plays a critical role in moving the catalytic domain across the endosomal membrane. Translocation/insertion is triggered by a decrease in pH in the endosome where conformational changes of T domain occur through several kinetic intermediates to yield a final trans-membr...

journal_title：Biochemistry

authors： Li J,Rodnin MV,Ladokhin AS,Gross ML

更新日期：2014-11-04 00:00:00

abstract：:Chloramphenicol acetyltransferase (CAT) reversibly transfers an acetyl group between CoA and the 3-hydroxyl of either chloramphenicol (Cm) or 1-acetylchloramphenicol (1AcCm). The products of the forward reactions, 3-acetylchloramphenicol (3-AcCm) and 1,3-diacetylchloramphenicol (1,3Ac2-Cm), are the substrates for the ...

journal_title：Biochemistry

authors： Murray IA,Derrick JP,White AJ,Drabble K,Wharton CW,Shaw WV

更新日期：1994-08-23 00:00:00

abstract：:Four mechanisms for the allosteric regulation of the calcium and magnesium ion activated adenosinetriphosphatase (Ca,Mg-ATPase) of sarcoplasmic reticulum were examined. Negative cooperativity in substrate binding was not supported by 3H-labeled 5'-adenylyl methylenediphosphate (AMPPCP) binding, which was best fit by a...

journal_title：Biochemistry

authors： Cable MB,Feher JJ,Briggs FN

更新日期：1985-09-24 00:00:00

abstract：:Neuronal specification is regulated by the activity of transcription factors containing the basic helix-loop-helix motif (bHLH); these regulating proteins include, among others, the neurogenin (Ngn) family, related to the atonal family of genes. Neurogenin 1 (NGN1) is a 237-residue protein that contains a bHLH domain ...

journal_title：Biochemistry

authors： Aguado-Llera D,Goormaghtigh E,de Geest N,Quan XJ,Prieto A,Hassan BA,Gómez J,Neira JL

更新日期：2010-03-02 00:00:00

abstract：:The arrangement of the mRNA on the Escherichia coli ribosome with respect to ribosomal RNA sites has been investigated by photochemical cross-linking experiments. mRNA analogues 51-54 nucleotides in length contained a Shine-Dalgarno sequence, a single codon for tRNA(Gly), and 4-thiouridine (s4U) in the 5' third of the...

journal_title：Biochemistry

authors： Bhangu R,Juzumiene D,Wollenzien P

更新日期：1994-03-15 00:00:00

abstract：:Type VI collagen, prepared from guanidine extracts of human amnion, contains very little monomeric material, the major forms being dimers and tetramers. In order to study the orientation of the molecules in these aggregates, they were digested with pepsin followed by bacterial collagenase. Two fragments were isolated,...

journal_title：Biochemistry

authors： Kuo HJ,Keene DR,Glanville RW

更新日期：1989-05-02 00:00:00

abstract：:Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...

journal_title：Biochemistry

authors： Da Silva GF,Harrison JS,Lai JR

更新日期：2010-07-06 00:00:00

abstract：:1H NMR and ligand-binding data were used to assess the role of residue Arg(E10) in ligand stabilization of several site-directed mutants, all carrying the His(E7) to Val substitution, obtained using a synthetic sperm whale myoglobin gene. Arg(E10) was previously found to form a hydrogen bond with the ligand in fluoro-...

journal_title：Biochemistry

authors： Allocatelli CT,Cutruzzolà F,Brancaccio A,Brunori M,Qin J,La Mar GN

更新日期：1993-06-15 00:00:00

abstract：:To identify interactions between Ste2p, a G protein-coupled receptor of the yeast Saccharomyces cerevisiae, and its tridecapeptide ligand, alpha-factor (WHWLQLKPGQPMY), a variety of alpha-factor analogues were used in conjunction with site-directed mutagenesis of a targeted portion of Ste2p transmembrane domain six. A...

journal_title：Biochemistry

authors： Lee BK,Lee YH,Hauser M,Son CD,Khare S,Naider F,Becker JM

更新日期：2002-11-19 00:00:00

abstract：:The beta-glucosidase from Agrobacterium faecalis (previously Alcaligenes faecalis) has been subjected to a detailed kinetic investigation with a range of substrates to probe its specificity and mechanism. It has a relatively broad specificity for the substrate sugar moiety and exhibits a classical pH dependence for it...

journal_title：Biochemistry

authors： Kempton JB,Withers SG

更新日期：1992-10-20 00:00:00

abstract：:Well-ordered multilamellar arrays of liquid-crystalline phosphatidylcholine and equimolar phosphatidylcholine-cholesterol bilayers have been formed in the nonaqueous solvents formamide and 1,3-propanediol. The organization of these bilayers and the interactions between apposing bilayer surfaces have been investigated ...

journal_title：Biochemistry

authors： McIntosh TJ,Magid AD,Simon SA

更新日期：1989-09-19 00:00:00

abstract：:The binding of epidermal growth factor (EGF) to epidermal growth factor receptor (EGF receptor) induces dimerization of the receptor and activation of its protein tyrosine kinase. Each of these three steps was followed as a function of the concentrations of EGF and of EGF receptor. Binding of EGF was followed by sedim...

journal_title：Biochemistry

authors： Sherrill JM,Kyte J

更新日期：1996-05-07 00:00:00

abstract：:The C-terminal domain from the muscle protein troponin C (TnC) comprises two helix-loop-helix calcium-binding sites (residues 90-162). The assembly of these two sites is governed by calcium binding enabling a synthetic C-terminal domain to be preferentially and stoichiometrically assembled from two synthetic peptides ...

journal_title：Biochemistry

authors： Shaw GS,Sykes BD

更新日期：1996-06-11 00:00:00

abstract：:Structural and genetic studies indicate that the antibacterial compound triclosan, an additive in many personal care products, is an inhibitor of EnvM, the enoyl reductase from Escherichia coli. Here we show that triclosan specifically inhibits InhA, the enoyl reductase from Mycobacterium tuberculosis and a target for...

journal_title：Biochemistry

authors： Parikh SL,Xiao G,Tonge PJ

更新日期：2000-07-04 00:00:00

abstract：:The thermotropic behavior of aqueous dispersions of stearoylsphingomyelin-cholesterol mixtures was examined by high-sensitivity differential scanning calorimetry. When less than 20 mol % cholesterol was mixed with the sphingomyelin and the samples were held at room temperature for 7-9 days before the initiation of cal...

journal_title：Biochemistry

authors： Estep TN,Freire E,Anthony F,Barenholz Y,Biltonen RL,Thompson TE

更新日期：1981-12-08 00:00:00

abstract：:Streptomyces avermitilis, an industrial organism responsible for the production of the anthelminthic avermectins, harbors a 13.4 kb gene cluster containing 13 unidirectionally transcribed open reading frames corresponding to the apparent biosynthetic operon for the sesquiterpene antibiotic pentalenolactone. The advanc...

journal_title：Biochemistry

authors： Tetzlaff CN,You Z,Cane DE,Takamatsu S,Omura S,Ikeda H

更新日期：2006-05-16 00:00:00

abstract：:The reactivity of the mitochondrial (pI = 5) isoenzyme of horse liver aldehyde dehydrogenase was determined by studying the effects of pH on steady-state velocity, burst magnitude, and molecular weight of the enzyme in the absence and presence of Mg2+ ions. The Mg2+ ion activation of the steady-state velocity at pH 7....

journal_title：Biochemistry

authors： Takahashi K,Weiner H,Filmer DL

更新日期：1981-10-13 00:00:00

abstract：:Cibacron Blue F3GA, the polycyclic blue chromophore of Blue Dextran 2000, inhibits the catalytic subunit of bovine brain protein kinase. The rate of inactivation exhibits a hyperbolic dependence on the dye concentration. This suggests that an enzyme-dye complex forms prior to inactivation. Protein and peptide substrat...

journal_title：Biochemistry

authors： Witt JJ,Roskoski R Jr

更新日期：1980-01-08 00:00:00

abstract：:The use of cefoxitin, a poor substrate of the RTEM beta-lactamase, has allowed the kinetic and spectroscopic characterization of a covalent acyl-enzyme intermediate in the enzyme-catalyzed reaction. The rate of reappearance of catalytic activity in an enzyme sample diluted from an incubation with cefoxitin is nearly i...

journal_title：Biochemistry

authors： Fisher J,Belasco JG,Khosla S,Knowles JR

更新日期：1980-06-24 00:00:00

abstract：:Static FTIR light-induced difference spectra have been recorded for reaction centers from Rhodopseudomonas viridis in the following charge-separated states: P+QA(-)-PQA, P+QB(-)-PQB, I(-)-I, I-QA(-)-IQA, and I-QA(2-)-IQA. A comparison of the I(-)-I difference spectra with the I-QA(-)-IQA difference spectra reveals new...

journal_title：Biochemistry

authors： Buchanan S,Michel H,Gerwert K

更新日期：1992-02-11 00:00:00

abstract：:VSTx1 is a voltage sensor toxin from the spider Grammostola spatulata that inhibits KvAP, an archeabacterial voltage-activated K(+) channel whose X-ray structure has been reported. Although the receptor for VSTx1 and the mechanism of inhibition are unknown, the sequence of the toxin is related to hanatoxin (HaTx) and ...

journal_title：Biochemistry

authors： Jung HJ,Lee JY,Kim SH,Eu YJ,Shin SY,Milescu M,Swartz KJ,Kim JI

更新日期：2005-04-26 00:00:00

abstract：:Hydrogen-bonding and ionic interactions play fundamental roles in macromolecular recognition and function. In contrast to lysines and arginines, how histidines mediate these interactions is less well-understood due to the unique properties of its side chain imidazole that include an aromatic ring with two titratable n...

journal_title：Biochemistry

authors： Sepuru KM,Rajarathnam K

更新日期：2018-10-16 00:00:00

abstract：:Crowding, which characterizes the interior of all living cells, has been shown to dramatically affect biochemical processes, leading to stabilization of compact morphologies, enhanced macromolecular associations, and altered reaction rates. Due to the crowding-mediated shift in binding equilibria toward association, c...

journal_title：Biochemistry

authors： Goobes R,Kahana N,Cohen O,Minsky A

更新日期：2003-03-04 00:00:00