Inhibition of protein phosphatase 2A activity by selective electrophile alkylation damage.

abstract：:The mechanism of binding of reduced 1-(2-carboxyphenylamino)-1-deoxyribulose 5-phosphate (rCdRP) to two different binding sites on the bifunctional enzyme is determined by kinetic studies, using temperature-jump and stopped-flow equipment with fluorescence detection. Two rapid binding processes and a comparatively slo...

journal_title：Biochemistry

authors： Cohn W,Kirschner K,Paul C

更新日期：1979-12-25 00:00:00

abstract：:Understanding the set of rules which dictate how the primary amino acid sequence determines tertiary structure is an unsolved problem in biophysics. If it were possible to simultaneously measure all of the intramolecular distances in a protein (in real time) during a folding reaction, the "second" genetic code problem...

journal_title：Biochemistry

authors： Lillo MP,Szpikowska BK,Mas MT,Sutin JD,Beechem JM

更新日期：1997-09-16 00:00:00

abstract：:The diffusion of protons at the immediate vicinity of (less than 10 A from) a phospholipid membrane is studied by the application of the laser-induced proton pulse. A light-sensitive proton emitter (8-hydroxypyrene-1,3,6-trisulfonate) was trapped exclusively in the hydration layers of multilamellar vesicles made of eg...

journal_title：Biochemistry

authors： Gutman M,Nachliel E,Moshiach S

更新日期：1989-04-04 00:00:00

abstract：:A substrate analogue, 6-(difluoromethyl)tryptophan, was developed and characterized for mechanistic investigation of tryptophanase. The utility of this derivative was based on its ability to partition between fluoride elimination and carbon-carbon bond scission during tryptophan metabolism. The non-enzymatic hydrolysi...

journal_title：Biochemistry

authors： Woolridge EM,Rokita SE

更新日期：1991-02-19 00:00:00

abstract：:The level of albumin mRNA in the normal Buffalo rat liver and Morris hepatoma 7777 was compared by molecular hybridization with albumin complementary DNA (cDNA) and translational assays. Albumin mRNA was found to be 10% of the total rat liver poly(A)-containing RNA population but reduced approximately fourfold in the ...

journal_title：Biochemistry

authors： Tse TP,Morris HP,Taylor JM

更新日期：1978-07-25 00:00:00

abstract：:Our previous studies revealed that interaction of fibrin with the very low density lipoprotein (VLDL) receptor plays a prominent role in transendothelial migration of leukocytes and thereby inflammation. The major goal of our subsequent studies is to establish the structural basis for this interaction. As the first st...

journal_title：Biochemistry

authors： Banerjee K,Yakovlev S,Gruschus JM,Medved L,Tjandra N

更新日期：2018-07-31 00:00:00

abstract：:Membrane preparations from two independent ouabain-resistant HeLa cell clones, HI-B1 and HI-C1, each appear to contain two species of (Na,K)ATPase. Two-thirds of the total (Na,K)ATPase in each mutant is indistinguishable from the enzyme in preparations of wild type cells with respect to ouabain binding, ouabain inhibi...

journal_title：Biochemistry

authors： Robbins AR,Baker RM

更新日期：1977-11-15 00:00:00

abstract：:The baker's yeast saccharopine dehydrogenase (EC 1.5.1.7) was inactivated by 2,3-butanedione following pseudo-first-order reaction kinetics. The pseudo-first-order rate constant for inactivation was linearly related to the butanedione concentration, and a value of 7.5 M-1 min-1 was obtained for the second-order rate c...

journal_title：Biochemistry

authors： Fujioka M,Takata Y

更新日期：1981-02-03 00:00:00

abstract：:Hydrolysis of monodisperse short chain phosphatidylcholines, far below their critical micelle concentration, by phospholipase A2 (PLA2) and other interfacial enzymes is characterized. Results show that virtually all the observed hydrolysis by pancreatic and human inflammatory PLA2 occurs on surfaces of the reaction ve...

journal_title：Biochemistry

authors： Yu BZ,Berg OG,Jain MK

更新日期：1999-08-10 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:The rate of depolymerization of microtubules upon lowering the temperature was found to depend on the amount of time elapsed since the beginning of the assembly process. In the first minutes following self-assembly at 37 degrees C, microtubules are more cold sensitive and depolymerize faster than later at the steady s...

journal_title：Biochemistry

authors： Bonne D,Pantaloni D

更新日期：1982-03-02 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of d-glyceraldehyde 3-phosphate (GAP) into d-glycerate 1,3-bisphosphate (1,3-diPG) in the presence of NAD(+) and inorganic phosphate (P(i)). Within the active site, two anion-binding sites were ascribed to the binding o...

journal_title：Biochemistry

authors： Castilho MS,Pavão F,Oliva G,Ladame S,Willson M,Périé J

更新日期：2003-06-17 00:00:00

abstract：:The interaction of serine protease (esterases) with 6-chloro-2-pyrones was investigated. Time-dependent inactivation of chymotrypsin, alpha-lytic protease, pig liver elastase, and cholinesterase was found with 3- and 5-benzyl-6-chloro-2-pyrone, as well as 3- and 5-methyl-6-chloro-2-pyrone. No inactivation was observed...

journal_title：Biochemistry

authors： Westkaemper RB,Abeles RH

更新日期：1983-06-21 00:00:00

abstract：:The cardiac and skeletal muscle sarcoplasmic reticulum ryanodine receptor Ca(2+) release channels contain thiols that are potential targets of endogenously produced reactive oxygen and nitrogen intermediates. Previously, we showed that the skeletal muscle ryanodine receptor (RyR1) has O(2)-sensitive thiols; only when ...

journal_title：Biochemistry

authors： Sun J,Yamaguchi N,Xu L,Eu JP,Stamler JS,Meissner G

更新日期：2008-12-30 00:00:00

abstract：:Protein misfolding due to missense mutations is a common pathogenic mechanism in cystathionine β-synthase (CBS) deficiency. In our previous studies, we successfully expressed, purified, and characterized nine CBS mutant enzymes containing the following patient mutations: P49L, P78R, A114V, R125Q, E176K, R266K, P422L, ...

journal_title：Biochemistry

authors： Hnízda A,Majtan T,Liu L,Pey AL,Carpenter JF,Kodíček M,Kožich V,Kraus JP

更新日期：2012-06-12 00:00:00

abstract：:Optical detection of triplet-state magnetic resonance (ODMR) is employed to study the complexes formed between gene 32 protein (GP32), a single-stranded DNA-binding protein from bacteriophage T4, and the heavy-atom-derivatized polynucleotides poly(5-HgU) and poly(5-BrU). The triplet-state properties of some of the try...

journal_title：Biochemistry

authors： Khamis MI,Maki AH

更新日期：1986-10-07 00:00:00

abstract：:Aldehyde dehydrogenase 7A1 (ALDH7A1) is part of lysine catabolism and catalyzes the NAD(+)-dependent oxidation of α-aminoadipate semialdehyde to α-aminoadipate. Herein, we describe a structural study of human ALDH7A1 focused on substrate recognition. Five crystal structures and small-angle X-ray scattering data are re...

journal_title：Biochemistry

authors： Luo M,Tanner JJ

更新日期：2015-09-08 00:00:00

abstract：:We have examined in detail the kinetics of binding of the serpin alpha 2-antiplasmin to the serine proteases alpha-chymotrypsin and plasmin. These represent model systems for serpin binding. We find, in contrast to earlier published results with alpha 2-antiplasmin and plasmin, that binding is reversible, and slow bin...

journal_title：Biochemistry

authors： Longstaff C,Gaffney PJ

更新日期：1991-01-29 00:00:00

abstract：:Merozoite surface protein 2 (MSP2), an abundant glycosylphosphatidylinositol-anchored protein on the surface of Plasmodium falciparum merozoites, is a promising malaria vaccine candidate. MSP2 is intrinsically disordered and forms amyloid-like fibrils in solution under physiological conditions. The 25 N-terminal resid...

journal_title：Biochemistry

authors： Zhang X,Adda CG,Low A,Zhang J,Zhang W,Sun H,Tu X,Anders RF,Norton RS

更新日期：2012-02-21 00:00:00

abstract：:Ribonuclease P2 from the thermophilic archaebacterium Sulfolobus solfataricus is a small protein (7 kDa) with a known three-dimensional structure. Inspection of the structure and molecular dynamics simulation reveal that three aromatic residues (Phe5, Phe31, and Tyr33) from the hydrophobic core have a strong van der W...

journal_title：Biochemistry

authors： Mombelli E,Afshar M,Fusi P,Mariani M,Tortora P,Connelly JP,Lange R

更新日期：1997-07-22 00:00:00

abstract：:Phosphoenolthiopyruvate, the analogue of phosphoenolpyruvate in which the bridging oxygen of the phosphate ester is replaced by sulfur, has been synthesized from methyl acrylate and dimethyl (chlorothio)phosphonate. The compound is a substrate for alkaline phosphatase, pyruvate kinase, enolase, and phosphoenolpyruvate...

journal_title：Biochemistry

authors： Sikkema KD,O'Leary MH

更新日期：1988-02-23 00:00:00

abstract：:A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...

journal_title：Biochemistry

authors： Griep MA,Adkins BJ,Hromas D,Johnson S,Miller J

更新日期：1997-01-21 00:00:00

abstract：:From the larger set of 191 variants at all the variable contact positions in the turkey ovomucoid third domain, we selected a subset that consists of Asp, Glu, His, and Lys residues at eight of the nine contiguous P6-P3' positions (residues 13-21), the exception being P3-Cys16 which is involved in a conserved disulfid...

journal_title：Biochemistry

authors： Song J,Laskowski M Jr,Qasim MA,Markley JL

更新日期：2003-03-18 00:00:00

abstract：:Kinetics of flash-induced electron transfer from the soluble cytochrome c2 to the primary donor (P) of the reaction center purified from the purple bacterium Rhodobacter sphaeroides R-26 were investigated by time-resolved absorption spectroscopy. Re-reduction of P+ induced by a laser pulse was measured at 1283 nm both...

journal_title：Biochemistry

authors： Venturoli G,Mallardi A,Mathis P

更新日期：1993-12-07 00:00:00

abstract：:In circulation the phospholipid transfer protein (PLTP) facilitates the transfer of phospholipid-rich surface components from postlipolytic chylomicrons and very low density lipoproteins (VLDL) to HDL and thereby regulates plasma HDL levels. To study the molecular mechanisms involved in PLTP-mediated lipid transfer, w...

journal_title：Biochemistry

authors： Setälä NL,Holopainen JM,Metso J,Wiedmer SK,Yohannes G,Kinnunen PK,Ehnholm C,Jauhiainen M

更新日期：2007-02-06 00:00:00

abstract：:Human ferrochelatase (EC 4.99.1.1) catalyzes the insertion ferrous iron into protoporphyrin IX as the last step in heme biosynthesis, an essential process to most organisms given the vast intracellular functions of heme. Even with multiple ferrochelatase structures available, the exact mechanism for iron insertion int...

journal_title：Biochemistry

authors： Asuru AP,An M,Busenlehner LS

更新日期：2012-09-11 00:00:00

abstract：:In the present report we studied the interaction between the skeletal muscle ryanodine receptor and the ubiquitous S100A1 Ca2+ binding protein. S100A1 did not affect equilibrium [3H]ryanodine binding to purified rabbit skeletal muscle terminal cisternae at 100 microM free [Ca2+]. At nanomolar free [Ca2+], however, S10...

journal_title：Biochemistry

authors： Treves S,Scutari E,Robert M,Groh S,Ottolia M,Prestipino G,Ronjat M,Zorzato F

更新日期：1997-09-23 00:00:00

abstract：:trans-3-Chloroacrylic acid dehalogenase (CaaD) converts trans-3-chloroacrylic acid to malonate semialdehyde by the addition of H(2)O to the C-2, C-3 double bond, followed by the loss of HCl from the C-3 position. Sequence similarity between CaaD, an (alphabeta)(3) heterohexamer (molecular weight 47,547), and 4-oxalocr...

journal_title：Biochemistry

authors： Azurmendi HF,Wang SC,Massiah MA,Poelarends GJ,Whitman CP,Mildvan AS

更新日期：2004-04-13 00:00:00

abstract：:As a prerequisite to mammalian fertilization, the sperm acrosomal vesicle fuses with the plasma membrane and the acrosome contents are exocytosed. Induction occurs through engagement of the sperm receptors by multiple sugar residues. Multivalent polymers displaying mannose, fucose, or GlcNAc are effective synthetic in...

journal_title：Biochemistry

authors： Huang H,Rodolis MT,Bhatia SR,Sampson NS

更新日期：2017-06-06 00:00:00

abstract：:Biological membranes exhibit an asymmetric distribution of phospholipids. Phosphatidylserine (PS) is an acidic phospholipid that is found almost entirely on the interior of the cell where it is important for interaction with many cellular components. A less well understood phenomenon is the asymmetry of the neutral ph...

journal_title：Biochemistry

authors： Bazzi MD,Youakim MA,Nelsestuen GL

更新日期：1992-02-04 00:00:00