Abstract:
:The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzyme is tightly bound to the beta subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the beta subunit. The presence of zinc in beta is consistent with several other lines of evidence which indicate that this subunit is dirrectly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the beta subunit as well as the alpha and beta' subunits of the RNA polymerase.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Halling SM,Sanchez-Anzaldo FJ,Fukuda R,Doi RH,Meares CFdoi
10.1021/bi00632a012subject
Has Abstractpub_date
1977-06-28 00:00:00pages
2880-4issue
13eissn
0006-2960issn
1520-4995journal_volume
16pub_type
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