Producing positive, negative, and no cooperativity by mutations at a single residue located at the subunit interface in the aspartate receptor of Salmonella typhimurium.

abstract：:We report here that polyamide nucleic acid (PNA) as well as a polyamide nucleic acid-DNA chimera complementary to the primer binding site of the HIV-1 genome can completely block priming by tRNA3Lys and consequently the in vitro initiation of reverse transcription by HIV-1 RT. Conventional heating and cooling is not r...

journal_title：Biochemistry

authors： Lee R,Kaushik N,Modak MJ,Vinayak R,Pandey VN

更新日期：1998-01-20 00:00:00

abstract：:Peptides corresponding to portions of curaremimetic neurotoxin loop 2 and to a structurally similar segment of rabies virus glycoprotein were synthetically modified in order to gain information on structure-function relationships of neurotoxin loop 2 interactions with the acetylcholine receptor. Binding of synthetic p...

journal_title：Biochemistry

authors： Lentz TL

更新日期：1991-11-12 00:00:00

abstract：:Protein disulfide-isomerase, a protein localized to the lumen of the endoplasmic reticulum of eukaryotic cells, catalyzes the posttranslational formation and rearrangement of protein disulfide bonds. As isolated from bovine liver, the enzyme contains 0.8 free sulfhydryl group per mole of protein monomer and 3.1 disulf...

journal_title：Biochemistry

authors： Gilbert HF

更新日期：1989-09-05 00:00:00

abstract：:An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...

journal_title：Biochemistry

authors： Yano Y,Matsuzaki K

更新日期：2002-10-15 00:00:00

abstract：:DB2277, a heterocyclic diamidine, is a successful design for mixed base pair (bp) DNA sequence recognition. The compound has a central aza-benzimidazole group that forms two H-bonds with a GC bp that has flanking AT bps. The nuclear magnetic resonance structure of the DB2277-DNA complex with an AAGATA recognition site...

journal_title：Biochemistry

authors： Harika NK,Wilson WD

更新日期：2018-08-21 00:00:00

abstract：:In the native purple bacterial reaction center (RC), light-driven charge separation utilizes only the A-side cofactors, with the symmetry related B-side inactive. The process is initiated by electron transfer from the excited primary donor (P*) to the A-side bacteriopheophytin (P* --> P+ H(A)-) in approximately 3 ps. ...

journal_title：Biochemistry

authors： Kee HL,Laible PD,Bautista JA,Hanson DK,Holten D,Kirmaier C

更新日期：2006-06-13 00:00:00

abstract：:The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...

journal_title：Biochemistry

authors： Jin L,Yang J,Carey J

更新日期：1993-07-20 00:00:00

abstract：:The kinetics and thermodynamics of Al(III) exchange between aluminum citrate (AlL) and human serum transferrin were investigated in the 7.2-8.9 pH range. The C-site of human serum apotransferrin in interaction with bicarbonate removes Al(III) from Al citrate with an exchange equilibrium constant K1 = (2.0 +/- 0.6) x 1...

journal_title：Biochemistry

authors： Hémadi M,Miquel G,Kahn PH,El Hage Chahine JM

更新日期：2003-03-18 00:00:00

abstract：:Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor of the serpin superfamily which rapidly inactivates tissue plasminogen activator (tPA), but reacts with thrombin at a much slower rate. Based on the previous mutagenesis studies and the X-ray crystal structure of the thrombin E192Q-bovine pancrea...

journal_title：Biochemistry

authors： Rezaie AR

更新日期：1998-09-22 00:00:00

abstract：:Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...

journal_title：Biochemistry

authors： Laberge M,Osvath S,Fidy J

更新日期：2001-08-07 00:00:00

abstract：:The iron heme and its immediate environment can provide information that is pivotal to our understanding of the structural and mechanistic features that confer unusual properties to the heme peroxidases. X-ray absorption spectroscopy (XAS), which is ideally suited for the investigation of the local environment and ele...

journal_title：Biochemistry

authors： Sinclair R,Copeland B,Yamazaki I,Powers L

更新日期：1995-10-10 00:00:00

abstract：:As a light-driven water-plastoquinone oxidoreductase, Photosystem II produces molecular oxygen as an enzymatic product. Additionally, under a variety of stress conditions, reactive oxygen species are produced at or near the active site for oxygen evolution. In this study, Fourier-transform ion cyclotron resonance mass...

journal_title：Biochemistry

authors： Frankel LK,Sallans L,Limbach PA,Bricker TM

更新日期：2012-08-14 00:00:00

abstract：:Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA have been evolved to generate genetically encoded noncanonical amino acids (ncAAs). Use of tryptophan (Trp) analogues with pyrrole ring modification for their spatial and polarity tuning in enzyme activity and substrate specificity is still l...

journal_title：Biochemistry

authors： Jiang HK,Wang YH,Weng JH,Kurkute P,Li CL,Lee MN,Chen PJ,Tseng HW,Tsai MD,Wang YS

更新日期：2020-06-23 00:00:00

abstract：:Multielectron multiproton reactions play an important role in both biological systems and chemical reactions involved in energy storage and manipulation. A key strategy employed by nature in achieving such complex chemistry is the use of proton-coupled redox steps. Cytochrome c nitrite reductase (ccNiR) catalyzes the ...

journal_title：Biochemistry

authors： Judd ET,Stein N,Pacheco AA,Elliott SJ

更新日期：2014-09-09 00:00:00

abstract：:Structural changes in the complex formation between transducin and metarhodopsin II, the activated form of photolyzed rhodopsin, in visual transduction processes were analyzed by Fourier transform infrared spectroscopy. The spectrum of the complex was obtained by subtracting the contribution of metarhodopsin I and unc...

journal_title：Biochemistry

authors： Nishimura S,Sasaki J,Kandori H,Matsuda T,Fukada Y,Maeda A

更新日期：1996-10-15 00:00:00

abstract：:Scaffold/matrix-attached regions (S/MARs) are cis-acting elements with a function outside transcribed regions and in introns. Although they usually augment transcriptional rates, their action is highly context-dependent. We cloned an 800 bp S/MAR element from the upstream border of the human interferon-beta domain at ...

journal_title：Biochemistry

authors： Schübeler D,Mielke C,Maass K,Bode J

更新日期：1996-08-27 00:00:00

abstract：:Following ribonuclease digestion of methyl-3H-labeled B77 avian sarcoma virus RNA subunits, methylated oligonucleotides were isolated by diethylaminoethylcellulose chromotogrpahy. Partial nucleotide sequences were deduced from the known enzymatic specificities of the ribonucleases. In addition to methylated nucleoside...

journal_title：Biochemistry

authors： Dimock K,Stoltzfus CM

更新日期：1977-02-08 00:00:00

abstract：:Aminopeptidase B (APB, EC 3.4.11.6) preferentially hydrolyzes the N-terminal basic amino acids of synthetic and peptide substrates and requires a physiological concentration of NaCl for optimal activity. In this study, we used site-directed mutagenesis and molecular modeling to search for an amino acid residue that is...

journal_title：Biochemistry

authors： Ohnishi A,Watanabe J,Ogawa Y,Goto Y,Hattori A,Tsujimoto M

更新日期：2015-10-06 00:00:00

abstract：:The key polyamine catabolizing enzyme spermidine-spermine N1-acetyltransferase (SSAT) is among the few genes known to be inducible by the natural polyamines. Certain polyamine analogs markedly exaggerate this response and thus provide useful tools for studying the underlying regulatory mechanisms. As shown here, the a...

journal_title：Biochemistry

authors： Fogel-Petrovic M,Vujcic S,Brown PJ,Haddox MK,Porter CW

更新日期：1996-11-12 00:00:00

abstract：:Transcription of the human nonmuscle myosin II heavy chain-A (NMHC-A) gene is regulated via multiple elements located in intron 1, including element F which contains an E-box. In this study we have identified and characterized the factors that are capable of binding to element F. Yeast one-hybrid screening using eleme...

journal_title：Biochemistry

authors： Chung MC,Kim HK,Kawamoto S

更新日期：2001-07-31 00:00:00

abstract：:Over 70 transthyretin (TTR) mutations facilitate amyloidosis in tissues other than the central nervous system (CNS). In contrast, the D18G TTR mutation in individuals of Hungarian descent leads to CNS amyloidosis. D18G forms inclusion bodies in Escherichia coli, unlike the other disease-associated TTR variants overexp...

journal_title：Biochemistry

authors： Hammarström P,Sekijima Y,White JT,Wiseman RL,Lim A,Costello CE,Altland K,Garzuly F,Budka H,Kelly JW

更新日期：2003-06-10 00:00:00

abstract：:Sarcoplasmic phosphorylase phosphatase extracted from ground skeletal muscle was recovered in a high molecular weight from (Mr = 250000). This enzyme has been purified from extracts by anion-exchange and gel chromatography to yield a preparation with three major protein components of Mr 83000, 72000, and 32000 by sodi...

journal_title：Biochemistry

authors： Brautigan DL,Picton C,Fischer EH

更新日期：1980-12-09 00:00:00

abstract：:Catalytic promiscuity is a widespread, but poorly understood, phenomenon among enzymes with particular relevance to the evolution of new functions, drug metabolism, and in vitro biocatalyst engineering. However, there is at present no way to quantitatively measure or compare this important parameter of enzyme function...

journal_title：Biochemistry

authors： Nath A,Atkins WM

更新日期：2008-01-08 00:00:00

abstract：:Arginase is a binuclear Mn(2+) metalloenzyme that catalyzes the hydrolysis of L-arginine to L-ornithine and urea. X-ray crystal structures of arginase complexed to substrate analogues N(omega)-hydroxy-L-arginine and N(omega)-hydroxy-nor-L-arginine, as well as the products L-ornithine and urea, complete a set of struct...

journal_title：Biochemistry

authors： Cox JD,Cama E,Colleluori DM,Pethe S,Boucher JL,Mansuy D,Ash DE,Christianson DW

更新日期：2001-03-06 00:00:00

abstract：:DNA polymerases slide on DNA during replication, and the interface must be mobile for various conformational changes. The role of lubricant interfacial water is not understood. In this report, we systematically characterize the water dynamics at the interface and in the active site of a tight binding polymerase (pol β...

journal_title：Biochemistry

authors： Yang Y,Qin Y,Ding Q,Bakhtina M,Wang L,Tsai MD,Zhong D

更新日期：2014-08-26 00:00:00

abstract：:A conformational change in the DNA plasmid ColE1 appears to occur upon specific binding of the restriction endonuclease EcoRI. Enzyme association alters the chiral discrimination found in binding metallointercalators to DNA sites. The complexes tris(1,10-phenanthroline)ruthenium(II), Ru(phen)3(2+), tris(4,7-diphenyl-1...

journal_title：Biochemistry

authors： Barton JK,Paranawithana SR

更新日期：1986-04-22 00:00:00

abstract：:Theory for enzyme-catalyzed reactions is developed for the dependence on product concentration of deuterium isotope effects on V and V/K. Generally, a product that decreases the off-rate for a second product to zero causes the isotope effect on V/K to decrease to DKeq and that on V to decrease to a value between 1 and...

journal_title：Biochemistry

authors： Cook PF,Yoon MY,Hara S,McClure GD Jr

更新日期：1993-02-23 00:00:00

abstract：:Polylysine promoted extensive membrane mixing of liposomes only if the buffer pH was below the pKa of the lysyl residues. This observation suggested that fusion could be regulated in a physiological pH range if the homopolymer of L-histidine was substituted as fusogen. Microgram quantities of polyhistidine were added ...

journal_title：Biochemistry

authors： Uster PS,Deamer DW

更新日期：1985-01-01 00:00:00

abstract：:The experiments described here explore the role of local sequence in the folding of cellular retinoic acid binding protein I (CRABP I). This is a 136-residue, 10-stranded, antiparallel beta-barrel protein with seven beta-hairpins and is a member of the intracellular lipid binding protein (iLBP) family. The relative ro...

journal_title：Biochemistry

authors： Rotondi KS,Gierasch LM

更新日期：2003-07-08 00:00:00

abstract：:Eukaryotic proteins with carboxyl-terminal CaaX motifs undergo three post-translational processing reactions-protein prenylation, endoproteolysis, and carboxymethylation. Two genes in yeast encoding CaaX endoproteases, AFC1 and RCE1, have been identified. Rce1p is solely responsible for proteolysis of yeast Ras protei...

journal_title：Biochemistry

authors： Dolence JM,Steward LE,Dolence EK,Wong DH,Poulter CD

更新日期：2000-04-11 00:00:00