当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > TFEC can function as a transcriptional activator of the nonmuscle myosin II heavy chain-A gene in transfected cells.

TFEC can function as a transcriptional activator of the nonmuscle myosin II heavy chain-A gene in transfected cells.

Abstract：

:Transcription of the human nonmuscle myosin II heavy chain-A (NMHC-A) gene is regulated via multiple elements located in intron 1, including element F which contains an E-box. In this study we have identified and characterized the factors that are capable of binding to element F. Yeast one-hybrid screening using element F allowed isolation of cDNAs encoding transcriptional factors TFEC, TFE3, and USF2, each of which contains basic helix-loop-helix and leucine zipper motifs. Furthermore, cDNA cloning by polymerase chain reaction yielded cDNAs for two TFEC isoforms, designated TFEC-l and TFEC-s, which are generated by alternative pre-mRNA splicing. In addition to these four factors, USF1, which is known to share the same DNA binding elements with USF2, was isolated for comparison. Electrophoretic mobility shift assays and cotransfection studies of the expression constructs with reporter gene constructs revealed that the above five factors have different binding activities for element F with different transactivation potencies. USF1 and USF2 demonstrate the highest binding activity to element F, yet show the lowest element F-dependent transactivation. TFE3 has a high transactivation potency but the lowest binding activity. TFEC-l demonstrates a high binding activity with the highest transactivation potency, whereas TFEC-s has the same binding activity as TFEC-l with intermediate transactivation. We also demonstrate that an N-terminal activation domain exists only in TFEC-l, whereas a C-terminal activation domain is common to both the l and s isoforms. This study provides the first evidence of TFEC being an activator of transcription, with two separate activation domains.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Chung MC,Kim HK,Kawamoto S

doi

10.1021/bi002847d

subject

Has Abstract

pub_date

2001-07-31 00:00:00

pages

8887-97

issue

30

eissn

0006-2960

issn

1520-4995

pii

bi002847d

journal_volume

40

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Binding of adenosine diphosphoribosyltransferase to the termini and internal regions of linear DNAs.

    abstract：:Binding mechanisms of ADPR-transferase to restricted double-stranded DNA fragments of SV40 and pBR322 DNA were determined by nuclease protection techniques. Top and bottom strands of double-stranded DNA were identified by specific labeling with 32P. Protection against specific exonucleases identified binding of ADPR-t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00439a050

    authors： Sastry SS,Buki KG,Kun E

    更新日期：1989-06-27 00:00:00

  • Glutamate 329 located in the fourth transmembrane segment of the alpha-subunit of the rat kidney Na+,K+-ATPase is not an essential residue for active transport of sodium and potassium ions.

    abstract：:An allelic variant of the ouabain-insensitive rat kidney Na+,K(+)-ATPase alpha 1-isoform was identified by chance in a cDNA library. The variant differed from the wild-type rat kidney Na+,K(+)-ATPase by a single G to C base substitution in the cDNA, which on the amino acid level gave rise to a glutamine in place of th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00211a048

    authors： Vilsen B

    更新日期：1993-12-07 00:00:00

  • Assembly of a [2Fe-2S]2+ cluster in a molecular variant of Clostridium pasteurianum rubredoxin.

    abstract：:The rubredoxin from Clostridium pasteurianum contains a single iron atom bound to the polypeptide chain by cysteines 6, 9, 39, and 42. The C42A variant of this protein has been prepared by site-directed mutagenesis and heterologous expression of the gene in Escherichia coli. The mutated protein was found to contain an...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971775w

    authors： Meyer J,Gagnon J,Gaillard J,Lutz M,Achim C,Münck E,Pétillot Y,Colangelo CM,Scott RA

    更新日期：1997-10-28 00:00:00

  • Crystal structure of ribonuclease T1 carboxymethylated at Glu58 in complex with 2'-GMP.

    abstract：:The carboxymethylation of RNase T1 at the gamma-carboxyl group of Glu58 leads to a complete loss of the enzymatic activity while it retains substrate-binding ability. Accompanying the carboxymethylation, RNase T1 undergoes a remarkable thermal stabilization of 9 degrees C in the melting temperature (Tm). In order to c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960493d

    authors： Ishikawa K,Suzuki E,Tanokura M,Takahashi K

    更新日期：1996-06-25 00:00:00

  • Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase.

    abstract：:The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00540

    authors： McGuire AH,Carey LM,de Serrano V,Dali S,Ghiladi RA

    更新日期：2018-07-31 00:00:00

  • Ethanol-perturbed amyloidogenic self-assembly of insulin: looking for origins of amyloid strains.

    abstract：:A model cosolvent, ethanol, has profound and diversified effects on the amyloidogenic self-assembly of insulin, yielding spectroscopically and morphologically distinguishable forms of beta-aggregates. The alcohol reduces hydrodynamic radii of insulin molecules, decreases enthalpic costs associated with aggregation-pro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi050281t

    authors： Dzwolak W,Grudzielanek S,Smirnovas V,Ravindra R,Nicolini C,Jansen R,Loksztejn A,Porowski S,Winter R

    更新日期：2005-06-28 00:00:00

  • Cross-complexing pattern of plant histones.

    abstract：:Pea histones H2a, H2b, H3, and H4 have been isolated and their interactions studied by fluorescence anisotropy, light scatter, and circular dichroism. Histones H3 and H4 are almost identical in plants and animals, but plant histones H2a and H2b differ markedly from their mammalian counterparts. Pea H2b has a molecular...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00628a009

    authors： Spiker S,Isenberg I

    更新日期：1977-05-03 00:00:00

  • Trifluoroethanol promotes helix formation by destabilizing backbone exposure: desolvation rather than native hydrogen bonding defines the kinetic pathway of dimeric coiled coil folding.

    abstract：:We measure the effects of low concentrations of helix-stabilizing cosolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kinetics of folding of the dimeric alpha-helical coiled coil derived from the leucine zipper region of bZIP transcriptional activator GCN4. The change in kinetic behavior upon...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981641y

    authors： Kentsis A,Sosnick TR

    更新日期：1998-10-13 00:00:00

  • Conformational flexibility of luteinizing hormone-releasing hormone in aqueous solution. A carbon-13 spin-lattice relaxation time study.

    abstract：:The carbon-13 nuclear magnetic resonance (13C NMR) spectra of luteinizing hormone-releasing hormone (LH-RH) and lower homologous peptides have been assigned in aqueous solutions at various pH values. 13C spin-lattice relaxation times (T1) have been measured for all proton-bearing carbons at 25.2 and 67.9 MHz. From the...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00690a030

    authors： Deslauriers R,Levy GC,McGregor WH,Sarantakis K,Smith IC

    更新日期：1975-09-23 00:00:00

  • Solution conformation of the (+)-trans-anti-[BPh]dA adduct opposite dT in a DNA duplex: intercalation of the covalently attached benzo[c]phenanthrene to the 5'-side of the adduct site without disruption of the modified base pair.

    abstract：:Benzo[c]phenanthrene diol epoxide can covalently bind to the exocyclic amino group of deoxyadenosine to generate [BPh]dA adducts where the polycyclic aromatic hydrocarbon is attached to the major groove edge of DNA. This paper reports on NMR-energy minimization structural studies of the (+)-trans-anti-[BPh]dA adduct p...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00097a029

    authors： Cosman M,Fiala R,Hingerty BE,Laryea A,Lee H,Harvey RG,Amin S,Geacintov NE,Broyde S,Patel D

    更新日期：1993-11-23 00:00:00

  • Solution structure of the catalytic domain of human stromelysin-1 complexed to a potent, nonpeptidic inhibitor.

    abstract：:The full three-dimensional structure of the catalytic domain of human stromelysin-1 (SCD) complexed to a novel and potent, nonpeptidic inhibitor has been determined by nuclear magnetic resonance spectroscopy (NMR). To accurately mimic assay conditions, the structure was obtained in Tris buffer at pH 6.8 and without th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi981328w

    authors： Li YC,Zhang X,Melton R,Ganu V,Gonnella NC

    更新日期：1998-10-06 00:00:00

  • Rho transcription factor: symmetry and binding of bicyclomycin.

    abstract：:The antibiotic bicyclomycin inhibits rho-dependent termination processes by interfering with RNA translocation by preventing RNA binding at the translocation site or by uncoupling the translocation process from ATP hydrolysis. Previous studies have shown that bicyclomycin binds near the ATP hydrolysis pocket on rho. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000504+

    authors： Vincent F,Openshaw M,Trautwein M,Gaskell SJ,Kohn H,Widger WR

    更新日期：2000-08-08 00:00:00

  • Solution structure of GxTX-1E, a high-affinity tarantula toxin interacting with voltage sensors in Kv2.1 potassium channels .

    abstract：:GxTX-1E is a neurotoxin recently isolated from Plesiophrictus guangxiensis venom that inhibits the Kv2.1 channel in pancreatic beta-cells. The sequence of the toxin is related to those of previously studied tarantula toxins that interact with the voltage sensors in Kv channels, and GxTX-1E interacts with the Kv2.1 cha...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100246u

    authors： Lee S,Milescu M,Jung HH,Lee JY,Bae CH,Lee CW,Kim HH,Swartz KJ,Kim JI

    更新日期：2010-06-29 00:00:00

  • Catalysis of the oxidative folding of ribonuclease A by protein disulfide isomerase: dependence of the rate on the composition of the redox buffer.

    abstract：:The velocity of the oxidative renaturation of reduced ribonuclease A catalyzed by protein disulfide isomerase (PDI) is strongly dependent on the composition of a glutathione/glutathione disulfide redox buffer. As with the uncatalyzed, glutathione-mediated oxidative folding of ribonuclease, the steady-state velocity of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00217a004

    authors： Lyles MM,Gilbert HF

    更新日期：1991-01-22 00:00:00

  • Chemical modification of mitochondrial transhydrogenase: evidence for two classes of sulfhydryl groups.

    abstract：:Chemical-modification studies on submitochondrial particle pyridine dinucleotide transhydrogenase (EC 1.6.1.1) demonstrate the presence of one class of sulfhydryl group in the nicotinamide adenine dinucleotide phosphate (NADP) site and another peripheral to the active site. Reaction of the peripheral sulfhydryl group ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00615a015

    authors： Earle SR,O'Neal SG,Fisher RR

    更新日期：1978-10-31 00:00:00

  • Evolvability of thermophilic proteins from archaea and bacteria.

    abstract：:Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, b...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi400652c

    authors： Takano K,Aoi A,Koga Y,Kanaya S

    更新日期：2013-07-16 00:00:00

  • Librational motion of an "immobilized" spin label: hemoglobin spin labeled by a maleimide derivative.

    abstract：:The spin label Tempo-maleimide, when "immobilized" in hemoglobin, is shown to exhibit motional fluctuation whose amplitude and/or frequency depend on temperature and solution conditions. These motional fluctuations are observable by several electron spin resonance techniques. For desalted hemoglobin the fluctuations a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00600a014

    authors： Johnson ME

    更新日期：1978-04-04 00:00:00

  • Inhibition of phosphomannose isomerase by mercury ions.

    abstract：:Mercury ions can inhibit Candida albicans phosphomannose isomerase (PMI) by two different processes at sub-micromolar concentrations. Kinetic studies show that mercury ions are in rapid equilibrium with the enzyme and cause a clear partial noncompetitive inhibition when mannose 6-phosphate is used as the substrate. Th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00190a018

    authors： Wells TN,Payton MA,Proudfoot AE

    更新日期：1994-06-21 00:00:00

  • A conserved threonine within Escherichia coli leucyl-tRNA synthetase prevents hydrolytic editing of leucyl-tRNALeu.

    abstract：:Aminoacyl-tRNA synthetases ensure the fidelity of protein synthesis by accurately selecting and activating cognate amino acids for aminoacylation of the correct tRNA. Some tRNA synthetases have evolved an editing active site that is separate from the amino acid activation site providing two steps or "sieves" for amino...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi002915w

    authors： Mursinna RS,Lincecum TL Jr,Martinis SA

    更新日期：2001-05-08 00:00:00

  • Identification of the serine residue phosphorylated by protein kinase C in vertebrate nonmuscle myosin heavy chains.

    abstract：:Two-dimensional mapping of the tryptic phosphopeptides generated following in vitro protein kinase C phosphorylation of the myosin heavy chain isolated from human platelets and chicken intestinal epithelial cells shows a single radioactive peptide. These peptides were found to comigrate, suggesting that they were iden...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00218a012

    authors： Conti MA,Sellers JR,Adelstein RS,Elzinga M

    更新日期：1991-01-29 00:00:00

  • Interaction between the antiapoptotic protein Nr-13 and cytochrome c. Antagonistic effect of the BH3 domain of Bax.

    abstract：:Mitochondria act as a focal point for upstream apoptosis signals by releasing cytochrome c into the cytosol, leading to the activation of caspases and subsequent cell death. Members of the Bcl-2 protein family regulate this phenomenon by heterodimerization via the BH3 domain of proapoptotic members opposing their pro-...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0110286

    authors： Moradi-Améli M,Lorca T,Ficheux D,di Pietro A,Gillet G

    更新日期：2002-07-09 00:00:00

  • Physical and functional interactions of Caenorhabditis elegans WRN-1 helicase with RPA-1.

    abstract：:The Caenorhabditis elegans Werner syndrome protein, WRN-1, a member of the RecQ helicase family, has a 3'-5' DNA helicase activity. Worms with defective wrn-1 exhibit premature aging phenotypes and an increased level of genome instability. In response to DNA damage, WRN-1 participates in the initial stages of checkpoi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200791p

    authors： Hyun M,Park S,Kim E,Kim DH,Lee SJ,Koo HS,Seo YS,Ahn B

    更新日期：2012-02-21 00:00:00

  • A new pathway for transmembrane electron transfer in photosynthetic reaction centers of Rhodobacter sphaeroides not involving the excited special pair.

    abstract：:It is generally accepted that electron transfer in bacterial photosynthesis is driven by the first singlet excited state of a special pair of bacteriochlorophylls (P*). We have examined the first steps of electron transfer in a mutant of the Rhodobacter sphaeroides reaction center in which charge separation from P* is...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9703756

    authors： Van Brederode ME,Jones MR,Van Mourik F,Van Stokkum IH,Van Grondelle R

    更新日期：1997-06-10 00:00:00

  • Activation of Bordetella pertussis adenylate cyclase by the carboxyl-terminal tryptic fragment of calmodulin.

    abstract：:Highly purified tryptic fragments of calmodulin were tested for their ability to stimulate adenylate cyclase activity of Bordetella pertussis spheroplast membranes and were compared to their activities on brain Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. The C-terminal fragment, consisting of residu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00372a025

    authors： Wolff J,Newton DL,Klee CB

    更新日期：1986-12-02 00:00:00

  • Effect of membrane potential on band 3 conformation in the human erythrocyte membrane detected by triplet state quenching experiments.

    abstract：:The triplet lifetime and absorption anisotropy decay of eosin-labeled band 3 was measured in resealed erythrocyte ghosts. Membrane potentials were generated by the addition of valinomycin in the presence of a K+ gradient. Neither negative nor positive membrane potentials had any detectable effect on the rotational dif...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00134a016

    authors： Wyatt K,Cherry RJ

    更新日期：1992-05-19 00:00:00

  • Effect and cellular site of action of cysteine protease inhibitors on the cholesterol esterification pathway in macrophages and Chinese hamster ovary cells.

    abstract：:Stimulation of intracellular cholesterol esterification, which is catalyzed by the enzyme acyl-CoA:cholesterol O-acyltransferase (ACAT), by atherogenic lipoproteins in macrophages is a key step in the development of atheroma foam cells. Since other aspects of intracellular cholesterol metabolism involve proteolytic re...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00033a019

    authors： Schissel SL,Beatini N,Zha X,Maxfield FR,Tabas I

    更新日期：1995-08-22 00:00:00

  • Structural basis for substrate binding and the catalytic mechanism of type III pantothenate kinase.

    abstract：:Pantothenate kinase (PanK) catalyzes the first step of the universal five-step coenzyme A (CoA) biosynthetic pathway. The recently characterized type III PanK (PanK-III, encoded by the coaX gene) is distinct in sequence, structure and enzymatic properties from both the long-known bacterial type I PanK (PanK-I, exempli...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7018578

    authors： Yang K,Strauss E,Huerta C,Zhang H

    更新日期：2008-02-05 00:00:00

  • Structure and thermal denaturation of crystalline and noncrystalline cytochrome oxidase as studied by infrared spectroscopy.

    abstract：:Fourier-transform infrared spectroscopy has been applied to the study of lipid vesicle-supported two-dimensional crystals and noncrystalline preparations of beef heart cytochrome oxidase. At room temperature, no conformational differences are seen between the noncrystalline and crystalline proteins, whose conformation...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00204a029

    authors： Arrondo JL,Castresana J,Valpuesta JM,Goñi FM

    更新日期：1994-09-27 00:00:00

  • Modification of the conformational equilibria in the sodium and potassium dependent adenosinetriphosphatase with glutaraldehyde.

    abstract：:Glutaraldehyde treatment of electroplax membrane preparations of Na,K-ATPase leads to irreversible changes in the enzymic behavior of the protein, which are not due to modification of the active site. When the glutaraldehyde treatment is carried out in a medium containing K+ and without Na+, the "K+-modified enzyme" s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00288a016

    authors： Chipman DM,Lev A

    更新日期：1983-09-13 00:00:00

  • Pyrrolopyridine derivatives from pyridoxal 5'-sulfate.

    abstract：:A fluorescent derivative of lysine-258 isolated from the active site of aspartate aminotransferase modified by treatment of the apoenzyme with pyridoxal 5'-sulfate has been characterized as a substituted 2H-pyrrolo[3,4-c]pyridine. Similar pyrrolopyridines are produced in up to 20% yield by reaction of pyridoxal sulfat...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00264a016

    authors： Scott RD,Ueno H,Metzler DE

    更新日期：1982-10-12 00:00:00