Abstract：
:A fluorescent derivative of lysine-258 isolated from the active site of aspartate aminotransferase modified by treatment of the apoenzyme with pyridoxal 5'-sulfate has been characterized as a substituted 2H-pyrrolo[3,4-c]pyridine. Similar pyrrolopyridines are produced in up to 20% yield by reaction of pyridoxal sulfate with simple alkylamines or with amino acids including lysine. The latter forms two products, one of which is identical with that isolated from the enzyme. The pyrrolopyridine derived from ethylamine has been characterized by proton and 13C NMR, ultraviolet-visible, and mass spectroscopy and by its chemical reactions.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Scott RD,Ueno H,Metzler DEdoi
10.1021/bi00264a016subject
Has Abstractpub_date
1982-10-12 00:00:00pages
5213-9issue
21eissn
0006-2960issn
1520-4995journal_volume
21pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:P-Glycoprotein (Pgp) expression in cell lines derived from tumors arising from cells which normally express Pgp can be increased by sodium butyrate and other differentiating agents. Although the Pgp level increased 25-fold after sodium butyrate treatment in SW620 human colon carcinoma cells, the intracellular accumula...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a002
更新日期：1992-07-21 00:00:00
abstract：:Two antisera used in the radioimmunoassay for human fibrinopeptide A (FPA) which appear to have different immunochemical specificities have been tested for cross-reactivity with fibrinogen and with three fragments of fibrinogen which contain the FPA sequence. These fragments were the three-chain, NH2-terminal disulfid...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00651a004
更新日期：1976-03-23 00:00:00
abstract：:tRNase Z, which exists in almost all cells, is believed to be working primarily for tRNA 3' maturation. In Escherichia coli, however, the tRNase Z gene appears to be dispensable under normal growth conditions, and its physiological role is not clear. Here, to investigate a possibility that E. coli tRNase Z cleaves RNA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051972s
更新日期：2006-05-02 00:00:00
abstract：:For the elucidation of the mechanism of steroid hormone receptor activation, the hydrodynamic properties of the unactivated and activated forms of the nonproteolyzed glucocorticoid receptor from the mouse AtT-20 pituitary tumor cell line were determined. The unactivated, molybdate-stabilized receptor has the following...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00277a038
更新日期：1983-04-12 00:00:00
abstract：:Metal homeostasis and resistance in bacteria is maintained by a panel of metal-sensing transcriptional regulators that collectively control transition metal availability and mediate resistance to heavy metal xenobiotics, including As(III), Cd(II), Pb(II), and Hg(II). The ArsR family constitutes a superfamily of metal ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100490u
更新日期：2010-08-10 00:00:00
abstract：:The PLL(PTE-like lactonase)-group of enzymes within the amidohydrolase superfamily hydrolyze N-acyl-homoserine lactones (AHLs) that are involved in bacterial quorum-sensing pathways. These enzymes possess the (beta/alpha)(8)-barrel fold and serve as attractive templates for in vitro evolution and engineering of quorum...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9004045
更新日期：2009-05-26 00:00:00
abstract：:Although plasma membrane domains, such as caveolae, provide an organizing principle for signaling pathways and cholesterol homeostasis in the cell, relatively little is known regarding specific mechanisms, whereby intracellular lipid-binding proteins are targeted to caveolae. Therefore, the interaction between caveoli...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7002636
更新日期：2007-07-17 00:00:00
abstract：:We have purified the junctional acetylcholine receptor from normal rat skeletal muscle and compared its structure with that of the extrajunctional receptor from denervated muscle. The two receptors from leg muscle were distinguished by isoelectric focusing and by reaction with sera from patients with myasthenia gravis...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00582a028
更新日期：1979-07-24 00:00:00
abstract：:A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025692d
更新日期：2002-06-25 00:00:00
abstract：:Phosphofructokinase from Escherichia coli (EcPFK) is a homotetramer with four active sites and four allosteric sites. Understanding the allosteric activation of EcPFK by MgADP has been complicated by the complex web of possible interactions, including active site homotropic interactions, allosteric site homotropic int...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi048569q
更新日期：2004-11-09 00:00:00
abstract：:Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH diminished calci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9616209
更新日期：1996-10-15 00:00:00
abstract：:The mechanisms by which multisubunit histone acetyltransferase (HAT) complexes recognize and perform efficient acetylation on nucleosome substrates are largely unknown. Here, we use a variety of biochemical approaches and compare histone-based substrates of increasing complexity to determine the critical components of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602366n
更新日期：2007-02-27 00:00:00
abstract：:At neutral pH, compound I of Arthromyces ramosus peroxidase (ARP) was stable and was reduced to ferric ARP without apparent formation of compound II upon titration with ascorbate or hydroquinone. In the titration experiments, compound II was seen as an intermediate only at alkaline pH. However, measuring a difference ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00184a038
更新日期：1994-05-10 00:00:00
abstract：:Inactivation of rabbit lung angiotensin converting enzyme (ACE) by 1-fluoro-2,4-dinitrobenzene (Dnp-F) has been shown to be due primarily to the modification of a tyrosine residue [Bünning, P., Kleeman, S.G., & Riordan, J.F. (1990) Biochemistry (preceding paper in this issue)]. Rabbit testicular ACE is also inactivate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00498a011
更新日期：1990-11-20 00:00:00
abstract：:We have obtained a quantitative description of villin-nucleated actin polymerization in physiological salt by determining the concentrations of free villin (V), villin-actin monomer (VA), villin-actin dimer (VA2), and villin-actin oligomer (VAn). Over a range of actin-villin ratios from 0.1 to 20 we determined the con...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00383a019
更新日期：1987-05-05 00:00:00
abstract：:The cro repressor protein from bacteriophage lambda has been studied in solution by two-dimensional nuclear magnetic resonance spectroscopy (2D NMR). Following the approach of Wüthrich and co-workers [Wüthrich, K., Wider, G., Wagner, G., & Braun, W. (1982) J. Mol. Biol. 155, 311-319], individual spin systems were iden...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00338a011
更新日期：1985-08-13 00:00:00
abstract：:Effects of paramagnetic shift reagents on the 13C NMR spectra obtained from single-walled vesicle dispersions of egg phosphatidylcholine enriched with 13C in the N-methyl carbons are investigated. Spectra obtained at 25.1 MHz show that, at Yb3+ to phospholipid molar ratios as low as 0.06, complete resolution of the N-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00653a007
更新日期：1976-04-20 00:00:00
abstract：:The active site of histidine decarboxylase (HDC) from Lactobacillus 30a contains a pyruvoyl cofactor sitting at the interface of two molecules in a trimer. Although exhibiting hyperbolic kinetics at pH 4.8, near its optimum, HDC is cooperative at pH 7.6, indicating that the units of the trimer communicate. A Hill plot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00069a032
更新日期：1993-05-11 00:00:00
abstract：:Thermal unfolding of discoidal complexes of apolipoprotein (apo) C-1 with dimyristoyl phosphatidylcholine (DMPC) reveals a novel mechanism of lipoprotein stabilization that is based on kinetics rather than thermodynamics. Far-UV CD melting curves recorded at several heating/cooling rates from 0.047 to 1.34 K/min show ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025588w
更新日期：2002-06-11 00:00:00
abstract：:Transfection of cos-1 cells with either prostaglandin endoperoxide H synthase-1 (PGHS-1) or -2 (PGHS-2) results in a mixed population of cells containing a diverse range of expressed enzyme. The use of fluorescent substrates and antibodies, in conjunction with fluorescence microscopy, provides the means to quantitate ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00021a034
更新日期：1995-05-30 00:00:00
abstract：:Escherichia coli mannitol specific EII in membrane vesicles can be inhibited by the action of the oxidizable substrate-reduced phenazine methosulfate (PMS) in a manner similar to E. coli enzyme IIGlc [Robillard, G. T., & Konings, W. (1981) Biochemistry 20, 5025-5032]. The fact that reduced PMS and various oxidizing ag...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00297a006
更新日期：1984-01-17 00:00:00
abstract：:A high-molecular-weight glycoprotein has been purified from the cervical mucus of the bonnet monkey (Macaca radiata). The glycoprotein was shown to be homogeneous by electrophoresis, sedimentation equilibrium, and N-terminal group determination, and to contain 19% protein, 19% D-galactose, 18% N-acetyl-D-galactosamine...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00626a042
更新日期：1977-04-05 00:00:00
abstract：:The conformation of the GM3 ganglioside, Neu5Ac alpha 2-3Gal beta 1-4Glc beta 1-1 Cer, and its analogs containing the Neu5Gc or Neu4Ac5Gc residues (Gc = glycolyl, CH2OHCO) was investigated in Me2SO-d6 solution with the aid of a distance-mapping procedure based on rotating-frame NOE contacts, with hydroxyl protons bein...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00145a014
更新日期：1992-08-04 00:00:00
abstract：:While two-component systems (TCSs), composed of a sensor histidine kinase (HK) and a response regulator, are the main signaling pathways in bacteria, global TCS activity remains poorly described. Here, we report the kinetic parameters of the HK autophosphorylation reaction using previously uncharacterized γ-phosphate-...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00485
更新日期：2018-07-24 00:00:00
abstract：:Phosphotriesterase homology protein (PHP) is a member of a recently discovered family of proteins related to phosphotriesterase, a hydrolytic, bacterial enzyme with an unusual substrate specificity for synthetic organophosphate triesters and phosphorofluoridates, which are common constituents of chemical warfare agent...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi971707+
更新日期：1998-04-14 00:00:00
abstract：:Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051679s
更新日期：2006-01-24 00:00:00
abstract：:High-pressure 31P NMR was used for the first time to investigate the effects of pressure on the structure and dynamics of the phosphocholine headgroup in pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) multilamellar aqueous dispersions and in DPPC bilayers containing the positively charged form of the local an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00143a004
更新日期：1992-07-21 00:00:00
abstract：:Protein farnesyltransferase catalyzes the posttranslational farnesylation of several proteins involved in signal transduction, including Ras, and is a target enzyme for antitumor therapies. Efficient product formation catalyzed by protein farnesyltransferase requires an enzyme-bound zinc cation and high concentrations...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0011781
更新日期：2000-10-10 00:00:00
abstract：:Protein kinase C-related kinases (PRKs) are serine/threonine kinases that are members of the protein kinase C superfamily and can be activated by binding to members of the Rho family of small G proteins via a Rho binding motif known as an HR1 domain. The PRKs contain three tandem HR1 domains at their N-termini. The st...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi200039u
更新日期：2011-04-12 00:00:00
abstract：:ncd is a kinesin-related motor protein from Drosophila that moves in the opposite direction along microtubules to kinesin. To learn more about the ncd mechanism, ncd motor domain (R335-K700) was expressed in Escherichia coli and its enzymatic characteristics were studied. The ncd motor domain was purified from the cel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00040a042
更新日期：1995-10-10 00:00:00
