Role of metals in the reaction catalyzed by protein farnesyltransferase.

abstract：:P-glycoprotein (P-gp) is the most well-known ATP-binding cassette (ABC) transporter involved in unidirectional substrate translocation across the membrane lipid bilayer, thereby causing the typical multidrug resistance (MDR) phenotype expressed in many cancers. We observed that in human CEM acute lymphoblastic leukemi...

journal_title：Biochemistry

authors： Gayet L,Dayan G,Barakat S,Labialle S,Michaud M,Cogne S,Mazane A,Coleman AW,Rigal D,Baggetto LG

更新日期：2005-03-22 00:00:00

abstract：:Although the importance of interactions involving both the cytosolic and transmembrane regions of SecY and SecE has been documented, no information has been available for the physical contact sites of these translocase subunits in their cytosolic domains. We now carried out site-specific cross-linking experiments to i...

journal_title：Biochemistry

authors： Satoh Y,Mori H,Ito K

更新日期：2003-06-24 00:00:00

abstract：:There are at least nine neurodegenerative diseases associated with proteins that contain an unusually expanded polyglutamine domain, the best known of which is Huntington's disease. In all of these diseases, the mutant protein aggregates into neuronal inclusions; it is generally, although not universally, believed tha...

journal_title：Biochemistry

authors： Lee CC,Walters RH,Murphy RM

更新日期：2007-11-06 00:00:00

abstract：:The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...

journal_title：Biochemistry

authors： Schmalhofer WA,Bao J,McManus OB,Green B,Matyskiela M,Wunderler D,Bugianesi RM,Felix JP,Hanner M,Linde-Arias AR,Ponte CG,Velasco L,Koo G,Staruch MJ,Miao S,Parsons WH,Rupprecht K,Slaughter RS,Kaczorowski GJ,Garcia ML

更新日期：2002-06-18 00:00:00

abstract：:Malic enzyme from pigeon liver is alkylated by the substrate analogue bromopyruvate, resulting in the concomitant loss of its oxidative decarboxylase and oxalacetate decarboxylase activities, but not its ability to reduce alpha-keto acids. The inactivation of oxidative decarboxylase activity follows saturation kinetic...

journal_title：Biochemistry

authors： Chang GG,Hsu RY

更新日期：1977-01-25 00:00:00

abstract：:A recently discovered bifunctional antibiotic-resistance enzyme named AAC(3)-Ib/AAC(6')-Ib', from Pseudomonas aeruginosa, catalyzes acetylation of aminoglycoside antibiotics. Since both domains are acetyltransferases, each was cloned and purified for mechanistic studies. The AAC(3)-Ib domain appears to be highly speci...

journal_title：Biochemistry

authors： Kim C,Villegas-Estrada A,Hesek D,Mobashery S

更新日期：2007-05-01 00:00:00

abstract：:The binding of the R17 coat protein to synthetic RNAs containing one or two coat protein binding sites was characterized by using nitrocellulose filter and gel-retention assays. RNAs with two available sites bound coat protein in a cooperative manner, resulting in a higher affinity and reduced sensitivity to pH, ionic...

journal_title：Biochemistry

authors： Witherell GW,Wu HN,Uhlenbeck OC

更新日期：1990-12-18 00:00:00

abstract：:Osteopontin (OPN) is an acidic hydrophilic glycophosphoprotein that was first identified as a major sialoprotein in bones. It functions as a cell attachment protein displaying a RGD cell adhesion sequence and as a cytokine that signals through integrin and CD44 cell adhesion molecules. OPN is also implicated in human ...

journal_title：Biochemistry

authors： Platzer G,Schedlbauer A,Chemelli A,Ozdowy P,Coudevylle N,Auer R,Kontaxis G,Hartl M,Miles AJ,Wallace BA,Glatter O,Bister K,Konrat R

更新日期：2011-07-12 00:00:00

abstract：:The primary electron acceptor pheophytin (Pheo(D1)) plays a crucial role in regulation of forward and backward electron transfer in photosystem II (PSII). It is known that some cyanobacteria control the Pheo(D1) potential in high-light acclimation by exchanging the D1 proteins from different copies of the psbA genes. ...

journal_title：Biochemistry

authors： Shibuya Y,Takahashi R,Okubo T,Suzuki H,Sugiura M,Noguchi T

更新日期：2010-01-26 00:00:00

abstract：:Stopped-flow total intensity and anisotropy experiments on single tryptophan containing mutants of yeast phosphoglycerate kinase (PGK) located in either the carboxy-terminal domain (W308 and W333), amino-terminal domain (W48 and W122), or "hinge" region (W194 and W399) were performed. The results obtained for single t...

journal_title：Biochemistry

authors： Beechem JM,Sherman MA,Mas MT

更新日期：1995-10-24 00:00:00

abstract：:A covalently linked actin dimer is identified in solutions of actin prepared from an acetone powder from skeletal muscle. This actin dimer acts as an actin nucleating factor (ANF), decreasing the half-time for spontaneous actin polymerization. ANF reacts with antibodies to both the N- and C-terminal portions of actin ...

journal_title：Biochemistry

authors： Selden LA,Kinosian HJ,Estes JE,Gershman LC

更新日期：2000-01-11 00:00:00

abstract：:The rate of 1-palmitoyl-2-[12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino] dodecanoyl] phosphatidylcholine (P-C12-NBD-PC) transfer between dioleoylphosphatidylcholine vesicles was measured by a technique based on resonance energy transfer between P-C12-NBD-PC and N-(lissamine rhodamine B sulfonyl)dioleoylphosphatidyletha...

journal_title：Biochemistry

authors： Nichols JW

更新日期：1986-08-12 00:00:00

abstract：:The DNA binding domain (DBD) of p53 folds by a complex mechanism that involves parallel pathways and multiple intermediates, both on- and off-pathway. This heterogeneity renders DBD particularly susceptible to misfolding and aggregation. The origins of parallel folding mechanisms are not well understood. DBD folding h...

journal_title：Biochemistry

authors： Butler JS,Loh SN

更新日期：2007-03-13 00:00:00

abstract：:N-(1-Pyrenyl)iodoacetamide has been used to introduce fluorescent probes into Escherichia coli RNA polymerase. After an incubation time of 15 min, approximately 2 pyrene equiv was introduced per enzyme molecule. There was no further increase in modification after more extended periods of incubation. Neither calf thymu...

journal_title：Biochemistry

authors： Johnson RS,Bowers M,Eaton Q

更新日期：1991-01-08 00:00:00

abstract：:The dietary flavonoid quercetin has a broad range of biological activities, including potent antitumor activity against several types of tumors. Recently, it has been shown that quercetin inhibits cancer cells proliferation by depleting cellular microtubules and perturbing cellular microtubule functions. However, the ...

journal_title：Biochemistry

authors： Gupta K,Panda D

更新日期：2002-10-29 00:00:00

abstract：:The proteins which have been left tightly bound to the tissue culture substrate after ethylenebis (oxyethyl-enenitrilo) tetraacetic acid (EGTA)-mediated removal of normal, virus-transformed, and revertant mouse cells and which have been implicated in the substrate adhesion process have been analyzed by slab sodium dod...

journal_title：Biochemistry

authors： Culp LA

更新日期：1976-09-07 00:00:00

abstract：:The binding of the codon UUC to the isolated anticodon loop of tRNAPhe (yeast) has been studied as a model of codon recognition by a simple adaptor. Fluorescence titrations demonstrate that UUC binds to the isolated anticodon loop with an equilibrium constant of 1.4 X 10(3) M-1 (at 7.2 degrees C). Equilibrium sediment...

journal_title：Biochemistry

authors： Bujalowski W,Jung M,McLaughlin LW,Porschke D

更新日期：1986-10-21 00:00:00

abstract：:Through the application of a unique method for isolating plasma membranes, it was possible to specifically iodinate cytoplasm-exposed plasma membrane proteins in vegetative cells of the cellular slime mold Dictyostelium discoideum. The original procedure [Chaney, L. K., & Jacobson, B. S. (1983) J. Biol. Chem. 258, 100...

journal_title：Biochemistry

authors： Shiozawa JA,Jelenska MM,Jacobson BS

更新日期：1987-07-28 00:00:00

abstract：:The protective effect of cyclic stable nitroxide free radicals, having SOD-like activity, against oxidative damage was studied by using Escherichia coli xthA DNA repair-deficient mutant hypersensitive to H2O2. Oxidative damage induced by H2O2 was assayed by monitoring cell survival. The metal chelator 1,10-phenanthrol...

journal_title：Biochemistry

authors： Samuni A,Godinger D,Aronovitch J,Russo A,Mitchell JB

更新日期：1991-01-15 00:00:00

abstract：:The mechanism by which terminal guanidino nitrogen substituted analogues of amiloride inhibit Na-Ca exchange in purified cardiac sarcolemmal membrane vesicles has been investigated. These inhibitors block both Nai-dependent Ca2+ uptake and Nao-dependent Ca2+ efflux. Inhibition of Na-Ca exchange monitored in K+ is nonc...

journal_title：Biochemistry

authors： Slaughter RS,Garcia ML,Cragoe EJ Jr,Reeves JP,Kaczorowski GJ

更新日期：1988-04-05 00:00:00

abstract：:3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...

journal_title：Biochemistry

authors： Wu G,Raines DE,Miller KW

更新日期：1994-12-27 00:00:00

abstract：:A critical point of interaction between F(1) and F(0) in the bacterial F(1)F(0)-ATP synthase is formed by the alpha and delta subunits. Previous work has shown that the N-terminal domain (residues 3-105) of the delta subunit forms a 6 alpha-helix bundle [Wilkens, S., Dunn, S. D., Chandler, J., Dahlquist, F. W., and Ca...

journal_title：Biochemistry

authors： Wilkens S,Borchardt D,Weber J,Senior AE

更新日期：2005-09-06 00:00:00

abstract：:This paper presents the crystal structure of porcine pancreatic carboxypeptidase B (pp-CpB) in complex with a variety of thiol-based inhibitors that were developed as antagonists of activated thrombin-activatable fibrinolysis inhibitor (TAFIa). Recent studies have indicated that a selective inhibitor of TAFIa could en...

journal_title：Biochemistry

authors： Adler M,Bryant J,Buckman B,Islam I,Larsen B,Finster S,Kent L,May K,Mohan R,Yuan S,Whitlow M

更新日期：2005-07-05 00:00:00

abstract：:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...

journal_title：Biochemistry

authors： Serrano P,Hammond JA,Geralt M,Wüthrich K

更新日期：2018-03-13 00:00:00

abstract：:Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...

journal_title：Biochemistry

authors： Rajapaksa R,Abu-Soud H,Raushel FM,Harris BG,Cook PF

更新日期：1993-03-02 00:00:00

abstract：:Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin(1A) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the ...

journal_title：Biochemistry

authors： Pucadyil TJ,Kalipatnapu S,Harikumar KG,Rangaraj N,Karnik SS,Chattopadhyay A

更新日期：2004-12-21 00:00:00

abstract：:RS1, also known as retinoschisin, is an extracellular discoidin domain-containing protein that has been implicated in maintaining the cellular organization and synaptic structure of the vertebrate retina. Mutations in the gene encoding RS1 are responsible for X-linked retinoschisis, a retinal degenerative disease char...

journal_title：Biochemistry

authors： Dyka FM,Wu WW,Pfeifer TA,Molday LL,Grigliatti TA,Molday RS

更新日期：2008-09-02 00:00:00

abstract：:Neutral oligosaccharides, which accounted for 74% of the total N-linked sugar chains released by hydrazinolysis of rat small intestinal aminopeptidase N, were investigated on a structural basis. They are mainly composed of complex-type sugar chains with tri- and tetraantennary structures, and small amounts of high man...

journal_title：Biochemistry

authors： Takasaki S,Erickson RH,Kim YS,Kochibe N,Kobata A

更新日期：1991-09-17 00:00:00

abstract：:A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense. We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small p...

journal_title：Biochemistry

authors： Zhao W,Liang Y,Yan X,Yang L,Wang Q

更新日期：2020-09-29 00:00:00

abstract：:Rhodopsin kinase phosphorylates serine- and threonine-containing peptides from bovine rhodopsin's carboxyl-terminal sequence. Km's for the peptides decrease as the length of the peptide is increased over the range 12-31 amino acids, reaching 1.7 mM for peptide 318-348 from the rhodopsin sequence. The Km for phosphoryl...

journal_title：Biochemistry

authors： Palczewski K,Arendt A,McDowell JH,Hargrave PA

更新日期：1989-10-31 00:00:00