Codon-induced association of the isolated anticodon loop of tRNAPhe.

abstract：:Two hybridomas producing monoclonal antibodies to poly(adenosine diphosphate ribose) [poly(ADP-Rib)] were established. One antibody, 10H (IgG3, kappa), bound to most of the poly(ADP-Rib) preparation, which consisted of molecules of various sizes of more than 20 ADP-Rib residues. The binding of this antibody was inhibi...

journal_title：Biochemistry

authors： Kawamitsu H,Hoshino H,Okada H,Miwa M,Momoi H,Sugimura T

更新日期：1984-07-31 00:00:00

abstract：:A critical event in Alzheimer's disease is the transition of Abeta peptides from their soluble forms into disease-associated beta-sheet-rich conformers. Structural analysis of a complete D-amino acid replacement set of Abeta(1-42) enabled us to localize in the full-length 42-mer peptide the region responsible for the ...

journal_title：Biochemistry

authors： Janek K,Rothemund S,Gast K,Beyermann M,Zipper J,Fabian H,Bienert M,Krause E

更新日期：2001-05-08 00:00:00

abstract：:Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding s...

journal_title：Biochemistry

authors： van Ingen H,Lasonder E,Jansen JF,Kaan AM,Spronk CA,Stunnenberg HG,Vuister GW

更新日期：2004-01-13 00:00:00

abstract：:Heparin was isolated from an unusually large human hemangioma that contained an elevated level of mast cells. Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its structure and activity. Characterization of this human heparin included the following: one-d...

journal_title：Biochemistry

authors： Linhardt RJ,Ampofo SA,Fareed J,Hoppensteadt D,Mulliken JB,Folkman J

更新日期：1992-12-15 00:00:00

abstract：:Thioacetamide, a hepatocarcinogen and an inhibitor of heme synthesis, blocks the phenobarbitone-mediated increase in the transcription of cytochrome P-450b+e messenger RNA in rat liver. This property is also shared by CoCl2 and 3-amino-1,2,4-triazole, two other inhibitors of heme synthesis. Thus, it appears feasible t...

journal_title：Biochemistry

authors： Satyabhama S,Seelan RS,Padmanaban G

更新日期：1986-08-12 00:00:00

abstract：:RuvC is a well-characterized Holliday junction resolvase from E. coli. The presence of symmetry in its preferred recognition sequence leads to ambiguity in the position of the crossover point in the junction, because a symmetric junction can undergo branch migration. Symmetric immobile junctions are junctions that con...

journal_title：Biochemistry

authors： Sha R,Liu F,Iwasaki H,Seeman NC

更新日期：2002-09-10 00:00:00

abstract：:Photochemical and subsequent thermal reactions of rhodopsin containing 9-cis-retinal [Rh(9)] or one of four analogues with 9-cis geometries formed from ring-modified retinals, alpha-retinal [alpha Rh(9)], acyclic retinal [AcRh(9)], acyclic alpha-retinal [Ac alpha Rh(9)], and 5-isopropyl-alpha-retinal [P alpha Rh(9)] w...

journal_title：Biochemistry

authors： Okada T,Kandori H,Shichida Y,Yoshizawa T,Denny M,Zhang BW,Asato AE,Liu RS

更新日期：1991-05-14 00:00:00

abstract：:Apolipoproteins A-I and A-II form the major protein constituents of high-density lipid particles (HDL), the concentration of which is inversely correlated with the frequency of heart disease in humans. Although the physiological role of apolipoprotein A-II is unclear, evidence for its involvement in free fatty acid me...

journal_title：Biochemistry

authors： Kumar MS,Carson M,Hussain MM,Murthy HM

更新日期：2002-10-01 00:00:00

abstract：:Complex type N-glycosides of commercial bovine fetuin preparations from pooled fetal calf serum have been shown to contain comparable amounts of Gal4,4,4TRI (see structure A below) and Gal4,4,3TRI (structure B) as major asialo-structures. To investigate whether there is a clear genetic specificity for synthesis of the...

journal_title：Biochemistry

authors： Hayase T,Rice KG,Dziegielewska KM,Kuhlenschmidt M,Reilly T,Lee YC

更新日期：1992-05-26 00:00:00

abstract：:Prokaryotic and eukaryotic cytochrome c oxidases and several bacterial ubiquinol oxidases compose a superfamily of heme-copper oxidases. These enzymes are terminal components of aerobic respiratory chains, the principal energy-generating systems of aerobic organisms. Two such heme-copper oxidases are the aa3-type cyto...

journal_title：Biochemistry

authors： Calhoun MW,Thomas JW,Hill JJ,Hosler JP,Shapleigh JP,Tecklenburg MM,Ferguson-Miller S,Babcock GT,Alben JO,Gennis RB

更新日期：1993-10-12 00:00:00

abstract：:The X-ray crystal structures of the complexes of the recombinant kringle 1 domain of human plasminogen (Klpg) with the ligands epsilon-aminocaproic acid (EACA) and trans-4-(aminomethyl)cyclohexane-1-carboxylic acid (AMCHA), which are representative of a class of in vivo antifibrinolytic agents, have been determined at...

journal_title：Biochemistry

authors： Mathews II,Vanderhoff-Hanaver P,Castellino FJ,Tulinsky A

更新日期：1996-02-27 00:00:00

abstract：:Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...

journal_title：Biochemistry

authors： Kingsbury NL,Renegar RH,Chalovich JM

更新日期：2013-10-29 00:00:00

abstract：:MeCP2 has been identified as a chromatin-associated protein that recognizes MAR elements as well as methyl-CpGs. To characterize target sequences of MeCP2 in human cells, we employed two complementary methods. First, by use of a preparative chromatin immunoprecipitation protocol, we created from MCF7 cells a library e...

journal_title：Biochemistry

authors： Koch C,Strätling WH

更新日期：2004-05-04 00:00:00

abstract：:The solution structure of the actinorhodin acyl carrier protein (act apo-ACP) from the polyketide synthase (PKS) of Streptomyces coelicolor A3(2) has been determined using 1H NMR spectroscopy, representing the first polyketide synthase component for which detailed structural information has been obtained. Twenty-four ...

journal_title：Biochemistry

authors： Crump MP,Crosby J,Dempsey CE,Parkinson JA,Murray M,Hopwood DA,Simpson TJ

更新日期：1997-05-20 00:00:00

abstract：:Neutrophil elastase (NE), a mediator of inflammation, binds with high affinity numerous anionic molecules including suramin, a polysulfated naphthylurea, which inhibits it with a Ki of 0.2 microM and a 4:1 suramin:NE stoichiometry and thus constitutes a potential therapeutic agent. In an attempt to locate the suramin ...

journal_title：Biochemistry

authors： Mély Y,Cadène M,Sylte I,Bieth JG

更新日期：1997-12-16 00:00:00

abstract：:In spite of a nuclear targeting sequence, a substantial amount of dual-specificity tyrosine phosphorylation-regulated kinase (Dyrk1A) is located within the cytoplasm of neurons. Analysis of fractionated rat brains revealed that the majority of Dyrk1A was in the postnuclear precipitate. The kinase in this fraction was ...

journal_title：Biochemistry

authors： Murakami N,Bolton D,Hwang YW

更新日期：2009-10-06 00:00:00

abstract：:Mutation of polycystin-1 (PC1) is the major cause of autosomal dominant polycystic kidney disease. PC1 has a predicted molecular mass of ~460 kDa comprising a long multidomain extracellular N-terminal region, 11 transmembrane regions, and a short C-terminal region. Because of its size, PC1 has proven difficult to hand...

journal_title：Biochemistry

authors： Oatley P,Stewart AP,Sandford R,Edwardson JM

更新日期：2012-04-03 00:00:00

abstract：:We have carried out a comparative study of the effect of cholesterol on the thermotropic phase behavior of the distearoyl and dielaidoyl molecular species of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine using high-sensitivity differential scanning calorimetry. For both molecular species of pho...

journal_title：Biochemistry

authors： McMullen TP,McElhaney RN

更新日期：1997-04-22 00:00:00

abstract：:Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF ...

journal_title：Biochemistry

authors： Tao L,English AM

更新日期：2004-04-06 00:00:00

abstract：:Acid beta-glucosidase is a lysosomal membrane protein that cleaves the O-beta-D-glucosidic linkage of glucosylceramide and aryl-beta-glucosides. Full activity reconstitution of the pure enzyme requires phospholipids and saposin C, an 80 aa activator protein. The deficiency of the enzyme or activator leads to Gaucher d...

journal_title：Biochemistry

authors： Qi X,Grabowski GA

更新日期：1998-08-18 00:00:00

abstract：:We have used flash-detection optical and electron paramagnetic resonance spectroscopy to measure the kinetics and yield per flash of the photooxidation of cytochrome b559 and the yield per flash of the photooxidation of the tyrosine residue YD in Mn-depleted photosystem II (PSII) membranes at room temperature. The ini...

journal_title：Biochemistry

authors： Buser CA,Thompson LK,Diner BA,Brudvig GW

更新日期：1990-09-25 00:00:00

abstract：:L-Aspartate oxidase (Laspo) catalyzes the conversion of L-Asp to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). This bacterial pathway represents a potential drug target since it is absent in mammals. The Laspo R386L mutant was crystallized in the FAD-bound catalytically competent form and its t...

journal_title：Biochemistry

authors： Bossi RT,Negri A,Tedeschi G,Mattevi A

更新日期：2002-03-05 00:00:00

abstract：:The di-heme enzyme MauG catalyzes the oxidative biosynthesis of a tryptophan tryptophylquinone cofactor on a precursor of the enzyme methylamine dehydrogenase (preMADH). Reaction of H2O2 with the diferric form of MauG, or reaction of O2 with diferrous MauG, forms the catalytic intermediate known as bis-Fe(IV), which a...

journal_title：Biochemistry

authors： Yukl ET,Williamson HR,Higgins L,Davidson VL,Wilmot CM

更新日期：2013-12-31 00:00:00

abstract：:The intestinal fatty acid binding protein is composed of two beta-sheets surrounding a large interior cavity. There is a small helical domain associated with the portal for entry of the ligand into the cavity. Denaturation of the protein has been monitored in a residue-specific manner by collecting a series of two-dim...

journal_title：Biochemistry

authors： Hodsdon ME,Frieden C

更新日期：2001-01-23 00:00:00

abstract：:Diethyl pyrocarbonate (DEP) is an electrophilic reagent that is used to modify reversibly the histidine residues of proteins. Unfortunately, the lability of the acylated histidine adduct usually does not permit the isolation and identification of the modified histidine. By use of 500-MHz proton NMR spectroscopy, it ha...

journal_title：Biochemistry

authors： Altman J,Lipka JJ,Kuntz I,Waskell L

更新日期：1989-09-19 00:00:00

abstract：:The ubiquitous cyclophilins belong to peptidyl-prolyl cis/trans isomerases (PPIases; EC 5.2.1.8). They are able to catalyze the cis/trans isomerization about peptidyl-prolyl amide bonds. The mode of action of human cytosolic cyclophilin (Cyp18cy) has been studied on substrate analog tetrapeptide-4-nitroanilides contai...

journal_title：Biochemistry

authors： Schutkowski M,Wöllner S,Fischer G

更新日期：1995-10-10 00:00:00

abstract：:The cytochrome bc(1) complex from Paracoccus denitrificans and soluble fragments of its cytochrome c(1) and Rieske ISP subunits are characterized by a combined approach of protein electrochemistry and FTIR difference spectroscopy. The FTIR difference spectra provide information about alterations in the protein upon re...

journal_title：Biochemistry

authors： Ritter M,Anderka O,Ludwig B,Mäntele W,Hellwig P

更新日期：2003-10-28 00:00:00

abstract：:Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing ...

journal_title：Biochemistry

authors： Serrano P,Hammond JA,Geralt M,Wüthrich K

更新日期：2018-03-13 00:00:00

abstract：:Respiration, photosynthesis, and metabolism require the transfer of electrons through and between proteins over relatively long distances. It is critical that this electron transfer (ET) occur with specificity to avoid cellular damage, and at a rate that is sufficient to support the biological activity. A multistep ho...

journal_title：Biochemistry

authors： Choi M,Shin S,Davidson VL

更新日期：2012-09-04 00:00:00

abstract：:Structures of glycopeptides obtained by exhaustive Pronase digestion of high molecular weight (1.7 X 10(5)) salmon egg polysialoglycoprotein have been elucidated. Six principal glycopeptides isolated by gel chromatography and DEAE-Sephadex A-25 chromatography in the absence or presence of borate ion were analyzed for ...

journal_title：Biochemistry

authors： Shimamura M,Inoue Y,Inoue S

更新日期：1985-09-24 00:00:00