Isolation and characterization of human heparin.

abstract：:Dynamic light scattering measurements were carried out on secretin in aqueous solution (2 mM; pH 5.0). The results indicated that the molecule exists as a fairly compact hexamer under these solution conditions. Secondary structural properties of the secretin hexameric complex were evaluated using CD and NMR spectrosco...

journal_title：Biochemistry

authors： Carpenter KA,Schiller PW

更新日期：1998-12-01 00:00:00

abstract：:A manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from Arthrobacter globiformis strain CM-2 (MndD) cloned in Escherichia coli has been purified to homogeneity. Sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 Da). Steady-state kinetic analysis of Mnd...

journal_title：Biochemistry

authors： Whiting AK,Boldt YR,Hendrich MP,Wackett LP,Que L Jr

更新日期：1996-01-09 00:00:00

abstract：:Malate dehydrogenase occurs in virtually all eucaryotic cells in mitochondrial and cytoplasmic forms, both of which are composed of two identical subunits. The reactivation of the mitochondrial isoenzyme has been the subject of previous studies [Jaenicke, R., Rudolph, R., & Heider, I. (1979) Biochemistry 18, 1217-1223...

journal_title：Biochemistry

authors： Rudolph R,Fuchs I,Jaenicke R

更新日期：1986-04-08 00:00:00

abstract：:The effects of nearest neighbor interactions between a nucleotide base at the primer 3'-terminus and an incoming deoxyribonucleoside triphosphate on DNA polymerase catalyzed insertion were examined. Kinetics of inserting the fluorescent nucleotide analog 2-aminopurine deoxyribonucleotide (dAPMP) and dAMP opposite a te...

journal_title：Biochemistry

authors： Bloom LB,Otto MR,Beechem JM,Goodman MF

更新日期：1993-10-19 00:00:00

abstract：:The kinetics of human polymerase beta (pol beta) binding to the single-stranded DNA, in the (pol beta)(16) and (pol beta)(5) binding modes, that differ in the number of occluded nucleotide residues in the protein-DNA complexes, have been examined, using the fluorescence stopped-flow technique. This is the first determ...

journal_title：Biochemistry

authors： Rajendran S,Jezewska MJ,Bujalowski W

更新日期：2001-10-02 00:00:00

abstract：:The Vmax/Km (microM -1 min -1.) for bovine factor X activation by bovine factor IXa alpha, in the presence of sufficient [Ca2+] to saturate the initial reaction rate, was 0.007. When factor IXa beta was substituted for factor IXa alpha in this reaction, the Vmax/Km decreased to 0.001, suggesting that factor IXa alpha ...

journal_title：Biochemistry

authors： Link RP,Castellino FJ

更新日期：1983-08-16 00:00:00

abstract：:cDNAs encoding three cytochrome P-450 enzymes were cloned from a rabbit kidney cDNA library. These three cDNAs exhibit greater than 90% nucleotide sequence identity across the coding region. This degree of sequence identity is also seen with P450IVA4, an enzyme that catalyzes the omega-hydroxylation of prostaglandins ...

journal_title：Biochemistry

authors： Johnson EF,Walker DL,Griffin KJ,Clark JE,Okita RT,Muerhoff AS,Masters BS

更新日期：1990-01-30 00:00:00

abstract：:The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...

journal_title：Biochemistry

authors： Wiese A,Brandenburg K,Carroll SF,Rietschel ET,Seydel U

更新日期：1997-08-19 00:00:00

abstract：:The globular domain of chicken histone H1 (GH1) has been studied by 1H homonuclear and 1H-15N heteronuclear 2D NMR spectroscopy. After the full assignment of the proton and 15N resonances, the tertiary structure of GH1 was determined by an iterative procedure using distance geometry and restrained simulated annealing....

journal_title：Biochemistry

authors： Cerf C,Lippens G,Ramakrishnan V,Muyldermans S,Segers A,Wyns L,Wodak SJ,Hallenga K

更新日期：1994-09-20 00:00:00

abstract：:Mycobacterium tuberculosis and many other members of the Actinomycetes family produce mycothiol, i.e., 1-d-myo-inosityl-2-(N-acetyl-l-cysteinyl)amido-2-deoxy-alpha-d-glucopyranoside (MSH or AcCys-GlcN-Ins), to act against oxidative and antibiotic stress. The biosynthesis of MSH is essential for cell growth and has bee...

journal_title：Biochemistry

authors： Fan F,Luxenburger A,Painter GF,Blanchard JS

更新日期：2007-10-09 00:00:00

abstract：:Glucose oxidation to CO2 was investigated in isolated perifused rat epididymal fat cells. Insulin stimulated rates of oxidation up to 30-fold. Multiple pulses of insulin or prolonged perifusion with the hormone led to a time-dependent desensitization of the cells. The action of insulin could be mimicked by H2O2. Rever...

journal_title：Biochemistry

authors： Lipkin EW,Teller DC,de Haën C

更新日期：1983-02-15 00:00:00

abstract：:Pepsin, a gastric aspartic proteinase, is a zymogen-derived protein that undergoes irreversible alkaline denaturation at pH 6-7. Detailed knowledge of the structure of the alkaline-denatured state is an important step in understanding the mechanism of the formation of the active enzyme. An extensive analysis of the de...

journal_title：Biochemistry

authors： Konno T,Kamatari YO,Tanaka N,Kamikubo H,Dobson CM,Nagayama K

更新日期：2000-04-11 00:00:00

abstract：:The solution conformation of the ribonuclease barnase has been determined by using 1H nuclear magnetic resonance (NMR) spectroscopy. The 20 structures were calculated by using 853 interproton distance restraints obtained from analyses of two-dimensional nuclear Overhauser spectra, 72 phi and 53 chi 1 torsion angle res...

journal_title：Biochemistry

authors： Bycroft M,Ludvigsen S,Fersht AR,Poulsen FM

更新日期：1991-09-03 00:00:00

abstract：:The voltage-gated potassium channel, K(v)1.3, is a novel target for development of immunosuppressants. Using a functional (86)Rb(+) efflux assay, a new class of high-affinity K(v)1.3 inhibitors has been identified. The initial active in this series, 4-phenyl-4-[3-(2-methoxyphenyl)-3-oxo-2-azaprop-1-yl]cyclohexanone (P...

journal_title：Biochemistry

authors： Schmalhofer WA,Bao J,McManus OB,Green B,Matyskiela M,Wunderler D,Bugianesi RM,Felix JP,Hanner M,Linde-Arias AR,Ponte CG,Velasco L,Koo G,Staruch MJ,Miao S,Parsons WH,Rupprecht K,Slaughter RS,Kaczorowski GJ,Garcia ML

更新日期：2002-06-18 00:00:00

abstract：:Inosine has the ability to act as a "wild-card" binding nonspecifically to both A.T and G.C base pairs. This has obvious implications for the design of oligonucleotide site-directed probes. In this paper we present a series of oligonucleotides with a 5'pur9-pyr9-pyr9 motif which are designed to fold up sequentially in...

journal_title：Biochemistry

authors： Mills M,Völker J,Klump HH

更新日期：1996-10-15 00:00:00

abstract：:RNA:pseudouridine synthetase (Pus1) from Saccharomyces cerevisiae is a multisite specific enzyme that catalyzes the formation of pseudouridine at positions 34 and 36 of intron-containing precursor tRNAIle and at positions 27 and/or 28 of several yeast tRNAs. In this paper we demonstrate that the purified recombinant P...

journal_title：Biochemistry

authors： Arluison V,Hountondji C,Robert B,Grosjean H

更新日期：1998-05-19 00:00:00

abstract：:Homeodomain-only protein (HOP) is an 8-kDa transcriptional corepressor that is essential for the normal development of the mammalian heart. Previous studies have shown that HOP, which consists entirely of a putative homeodomain, acts downstream of Nkx2.5 and associates with the serum response factor (SRF), repressing ...

journal_title：Biochemistry

authors： Kook H,Yung WW,Simpson RJ,Kee HJ,Shin S,Lowry JA,Loughlin FE,Yin Z,Epstein JA,Mackay JP

更新日期：2006-09-05 00:00:00

abstract：:The O-linked protein glycosylation pathway in Neisseria gonorrhoeae is responsible for the synthesis of a complex oligosaccharide on undecaprenyl diphosphate and subsequent en bloc transfer of the glycan to serine residues of select periplasmic proteins. Protein glycosylation (pgl) genes have been annotated on the bas...

journal_title：Biochemistry

authors： Hartley MD,Morrison MJ,Aas FE,Børud B,Koomey M,Imperiali B

更新日期：2011-06-07 00:00:00

abstract：:Proteolytic processing of the transmembrane domain of the amyloid precursor protein (APP) is a key component of Alzheimer's disease pathogenesis. Using C-terminally tagged APP derivatives, we have identified by amino-terminal sequencing a novel cleavage site of APP, at Leu-49, distal to the gamma-secretase site. This ...

journal_title：Biochemistry

authors： Weidemann A,Eggert S,Reinhard FB,Vogel M,Paliga K,Baier G,Masters CL,Beyreuther K,Evin G

更新日期：2002-02-26 00:00:00

abstract：:Heme (iron protoporphyrin IX) is an essential protein prosthetic group and signaling molecule required for most life on Earth. All heme-dependent processes require the dynamic and rapid mobilization of heme from sites of synthesis or uptake to hemoproteins present in virtually every subcellular compartment. The cytoto...

journal_title：Biochemistry

authors： Hanna DA,Martinez-Guzman O,Reddi AR

更新日期：2017-04-04 00:00:00

abstract：:The synthesis of two potential arginine-directed purine-based analogues, 6-chloro-9-(3,4-dioxopentyl)purine (6) and 9-(3,4-dioxopentyl)hypoxanthine (7), is reported. Compound 7 was extensively tested as a potential affinity label of purine nucleoside phosphorylase (EC 2.4.2.1) from human erythrocytes. Evidence that 7 ...

journal_title：Biochemistry

authors： Salamone SJ,Jordan F

更新日期：1982-12-07 00:00:00

abstract：:By superimposing data obtained by photo-cross-linking RGD-containing ligands to the human alpha(V)beta(3) integrin onto the crystal structure of the ectopic domain of this receptor (Xiong et al. (2001) Science 294, 339-345), we have identified the binding site for the RGD triad within this integrin. We synthesized thr...

journal_title：Biochemistry

authors： Yahalom D,Wittelsberger A,Mierke DF,Rosenblatt M,Alexander JM,Chorev M

更新日期：2002-07-02 00:00:00

abstract：:alpha-Glucosidase (EC 3.2.1.20) was purified to homogeneity from logarithmically growing cells of Saccharomyces carlsbergensis. The purification involved the following steps: (a) ammonium sulfate fractionation; (b) Sephadex G-100 chromatography; (c) DEAE-cellulose chromatography; and (d) hydroxylapatite chromatography...

journal_title：Biochemistry

authors： Needleman RB,Federoff HJ,Eccleshall TR,Buchferer B,Marmur J

更新日期：1978-10-31 00:00:00

abstract：:Integrin conformational changes mediate integrin activation and signaling triggered by intracellular molecules or extracellular ligands. Even though it is known that αβ transmembrane domain separation is required for integrin signaling, it is still not clear how this signal is transmitted from the transmembrane domain...

journal_title：Biochemistry

authors： Wang W,Fu G,Luo BH

更新日期：2010-11-30 00:00:00

abstract：:Understanding the mechanisms of free energy transfer in metabolism is fundamental to understanding how the chemical forces that sustain the molecular organization of the cell are distributed. Recent studies of molecular motors (1-3) and ATP-driven proton transport (4-6) describe how chemical potential is transferred a...

journal_title：Biochemistry

authors： Wei J,Tang QX,Varlamova O,Roche C,Lee R,Leyh TS

更新日期：2002-07-02 00:00:00

abstract：:Melanoma growth stimulatory activity (MGSA) is a mitogenic protein secreted by Hs294T melanoma cells that corresponds to the polypeptide encoded by the human gro gene. The MGSA/gro cDNA has been expressed in mammalian cells and the secreted recombinant factor has been purified. Biochemical and biological characterizat...

journal_title：Biochemistry

authors： Balentien E,Han JH,Thomas HG,Wen DZ,Samantha AK,Zachariae CO,Griffin PR,Brachmann R,Wong WL,Matsushima K

更新日期：1990-11-06 00:00:00

abstract：:The rigor complexes that formed between rabbit skeletal muscle F-actin and chicken gizzard heavy meromyosin (HMM), in which the heavy chains had been cleaved with trypsin into 24K, 50K, and 68K fragments, were examined by using the zero-length chemical cross-linker 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide (EDC)...

journal_title：Biochemistry

authors： Onishi H,Maita T,Matsuda G,Fujiwara K

更新日期：1989-02-21 00:00:00

abstract：:We have determined the formal potential values for each electron transfer to electron transfer flavoprotein-ubiquinone oxidoreductase (ETF-QO), in order to further characterize the thermodynamics of electron transport from various acyl-CoA thioesters to the mitochondrial ubiquinone pool. ETF-QO contains one [4Fe-4S]2+...

journal_title：Biochemistry

authors： Paulsen KE,Orville AM,Frerman FE,Lipscomb JD,Stankovich MT

更新日期：1992-12-01 00:00:00

abstract：:Cytochrome oxidase proteoliposomes were prepared from bovine heart oxidase. Size distributions determined by quasi-elastic light scattering (QELS) showed that there was a small population of large vesicles (120-200-nm diameter) and a large population of small vesicles (50-100-nm diameter). Trapping cytochrome c inside...

journal_title：Biochemistry

authors： Cooper CE,Nicholls P

更新日期：1990-04-24 00:00:00

abstract：:Mycobacterial Ser/Thr kinase, PknB, is essential for the growth of the pathogen. Unphosphorylated PknB is catalytically inactive, and its activation requires autophosphorylation of Thr residues on the activation loop. Autophosphorylation can in principle take place via two distinct mechanisms. Intermolecular trans aut...

journal_title：Biochemistry

authors： Damle NP,Mohanty D

更新日期：2014-07-22 00:00:00