Purification and characterization of an alpha-glucosidase from Saccharomyces carlsbergensis.

abstract：:Human lysosomal enzymes acid-beta-glucosidase (GCase) and acid-alpha-galactosidase (alpha-Gal A) hydrolyze the sphingolipids glucosyl- and globotriaosylceramide, respectively, and mutations in these enzymes lead to the lipid metabolism disorders Gaucher and Fabry disease, respectively. We have investigated the structu...

journal_title：Biochemistry

authors： Lieberman RL,D'aquino JA,Ringe D,Petsko GA

更新日期：2009-06-09 00:00:00

abstract：:Tubulin polymerization promoting proteins (TPPPs) constitute a eukaryotic protein family. There are three TPPP paralogs in the human genome, denoted as TPPP1-TPPP3. TPPP1 and TPPP3 are intrinsically unstructured proteins (IUPs) that bind and polymerize tubulin and stabilize microtubules, but TPPP2 does not. Vertebrate...

journal_title：Biochemistry

authors： Oláh J,Szénási T,Szabó A,Kovács K,Lőw P,Štifanić M,Orosz F

更新日期：2017-02-21 00:00:00

abstract：:Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the terminal ca...

journal_title：Biochemistry

authors： Petrillo T,O'Donohoe CA,Howe N,Malthouse JP

更新日期：2012-08-07 00:00:00

abstract：:Desosamine is a 3-(dimethylamino)-3,4,6-trideoxyhexose found, for example, in such macrolide antibiotics as erthyromycin, azithromycin, and clarithromycin. The efficacies of these macrolide antibiotics are markedly reduced in the absence of desosamine. In the bacterium Streptomyces venezuelae, six enzymes are required...

journal_title：Biochemistry

authors： Burgie ES,Holden HM

更新日期：2007-08-07 00:00:00

abstract：:Kinetic studies of reactions between fluorescein and antifluorescein antibody produced during early, intermediate, and late stages of the immune response have been carried out utilizing both fluorescence intensity and polarization measurements in the static (time constant similar to 5 sec) and in the stopped-flow mode...

journal_title：Biochemistry

authors： Levison SA,Hicks AN,Portmann AJ,Dandliker WB

更新日期：1975-08-26 00:00:00

abstract：:Sensory rhodopsin I (SRI) is a seven-transmembrane helix retinylidene protein that mediates color-sensitive phototaxis responses through its bound transducer HtrI in the archaeon Halobacterium salinarum. Deprotonation of the Schiff base attachment site of the chromophore accompanies formation of the SRI signaling stat...

journal_title：Biochemistry

authors： Jung KH,Spudich EN,Dag P,Spudich JL

更新日期：1999-10-05 00:00:00

abstract：:The kinetics of charge recombination following photoexcitation by a laser pulse have been analyzed in the reaction center-light harvesting complex 1 (RC-LH1) purified from the photosynthetic bacterium Rhodobacter sphaeroides. In RC-LH1 core complexes isolated from photosynthetically grown cells P(+)Q(B)(-) recombines ...

journal_title：Biochemistry

authors： Francia F,Dezi M,Rebecchi A,Mallardi A,Palazzo G,Melandri BA,Venturoli G

更新日期：2004-11-09 00:00:00

abstract：:A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense. We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small p...

journal_title：Biochemistry

authors： Zhao W,Liang Y,Yan X,Yang L,Wang Q

更新日期：2020-09-29 00:00:00

abstract：:The inactivation of cytochrome P450 2B4 by aldehydes in a reconstituted enzyme system requires molecular oxygen and NADPH and is not prevented by the addition of catalase, superoxide dismutase, epoxide hydrolase, glutathione, or ascorbic acid. A strong correlation between loss of enzymatic activity and bleaching of th...

journal_title：Biochemistry

authors： Raner GM,Chiang EW,Vaz AD,Coon MJ

更新日期：1997-04-22 00:00:00

abstract：:Complementary DNA (cDNA) clones encoding bovine testicular 20 alpha-hydroxysteroid dehydrogenase (20 alpha-HSD) have been isolated from a bovine testicular lambda gt11 library using polyclonal antibodies against 20 alpha-HSD and DNA probe hybridization. Nucleotide sequencing of three independently isolated clones was ...

journal_title：Biochemistry

authors： Warren JC,Murdock GL,Ma Y,Goodman SR,Zimmer WE

更新日期：1993-02-16 00:00:00

abstract：:Adenylosuccinate synthetase catalyzes the first committed step in the de novo biosynthesis of AMP, coupling L-aspartate and IMP to form adenylosuccinate. Km values of IMP and 2'-deoxy-IMP are nearly identical with each substrate supporting comparable maximal velocities. Nonetheless, the Km value for L-aspartate and th...

journal_title：Biochemistry

authors： Iancu CV,Zhou Y,Borza T,Fromm HJ,Honzatko RB

更新日期：2006-09-26 00:00:00

abstract：:Poly(ethylene glycol) (PEG)-mediated fusion of phosphatidylcholine model membranes has been shown to mimic the protein-mediated biomembrane process [Lee, J., and Lentz, B. R. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 9274-9279]. Unlike the simple model membranes used in this earlier study, the lipid composition of fuso...

journal_title：Biochemistry

authors： Haque ME,McIntosh TJ,Lentz BR

更新日期：2001-04-10 00:00:00

abstract：:Unfractionated Escherichia coli tRNA has been aminoacylated with lysine and preferentially acetylated at the epsilon-amino nitrogen of lysine by reaction with N-acetoxysuccinimide. After treatment with peptidyl-tRNA hydrolase, 90% of the aminoacylated tRNA molecules were Nepsilon-acetyl-Lys-tRNA. Post-ribosomal supern...

journal_title：Biochemistry

authors： Johnson AE,Woodward WR,Herbert E,Menninger JR

更新日期：1976-02-10 00:00:00

abstract：:Allosteric activators are generally believed to shift the equilibrium distribution of enzyme conformations to favor a catalytically productive structure; the kinetics of conformational exchange is seldom addressed. Several observations suggested that the usual allosteric mechanism might not apply to the activation of ...

journal_title：Biochemistry

authors： Riera TV,Zheng L,Josephine HR,Min D,Yang W,Hedstrom L

更新日期：2011-10-04 00:00:00

abstract：:The present study was undertaken for the purpose of evaluating the solvent perturbation technique as a method to identify enzyme catalytic residues. For establishment of expected directions and sizes of pKa perturbations for different types of acids in different classes of solvents, a study of the pKa of a series of a...

journal_title：Biochemistry

authors： Grace S,Dunaway-Mariano D

更新日期：1983-08-30 00:00:00

abstract：:Sir2 protein deacetylases (or sirtuins) catalyze NAD+-dependent conversion of epsilon-amino-acetylated lysine residues to deacetylated lysine, nicotinamide, and 2'-O-acetyl-ADP-ribose. Small-molecule modulation of sirtuin activity might treat age-associated diseases, such as type II diabetes, obesity, and neurodegener...

journal_title：Biochemistry

authors： Smith BC,Denu JM

更新日期：2007-12-18 00:00:00

abstract：:The operations within a living cell depend on the collective activity of networks of proteins, sometimes termed "interactomes". Within these networks, most proteins interact with few partners, while a small proportion of proteins, called hubs, participate in a large number of interactions and play a central role in or...

journal_title：Biochemistry

authors： Barbar E

更新日期：2008-01-15 00:00:00

abstract：:The mechanisms of interaction of the recombinant N-terminal portion of bactericidal/permeability-increasing protein, rBPI21, with various planar asymmetric and symmetric bilayer membranes, including the lipid matrix of the outer membrane of Gram-negative bacteria, were investigated via electrical measurements. For the...

journal_title：Biochemistry

authors： Wiese A,Brandenburg K,Carroll SF,Rietschel ET,Seydel U

更新日期：1997-08-19 00:00:00

abstract：:Kinetic and structural data are presented on the interaction with Torpedo californica acetylcholinesterase (TcAChE) of (+)-huperzine A, a synthetic enantiomer of the anti-Alzheimer drug, (-)-huperzine A, and of its natural homologue (-)-huperzine B. (+)-Huperzine A and (-)-huperzine B bind to the enzyme with dissociat...

journal_title：Biochemistry

authors： Dvir H,Jiang HL,Wong DM,Harel M,Chetrit M,He XC,Jin GY,Yu GL,Tang XC,Silman I,Bai DL,Sussman JL

更新日期：2002-09-03 00:00:00

abstract：:The importance of the evolutionarily conserved Gly-4 residue for the affinity and kinetics of interaction of cystatin A with several cysteine proteinases was assessed by site-directed mutagenesis. Even the smallest replacement, by Ala, resulted in approximately 1000-, approximately 10- and approximately 6000-fold decr...

journal_title：Biochemistry

authors： Estrada S,Nycander M,Hill NJ,Craven CJ,Waltho JP,Björk I

更新日期：1998-05-19 00:00:00

abstract：:Sorcin, a 21.6 kDa cytosolic EF-hand protein which undergoes a Ca(2+)-induced translocation from cytoplasm to membranes, has been assigned to the newly defined penta EF-hand family. A molecular model of the C-terminal Ca(2+)-binding domain has been generated using as a template the X-ray coordinates of the correspondi...

journal_title：Biochemistry

authors： Zamparelli C,Ilari A,Verzili D,Giangiacomo L,Colotti G,Pascarella S,Chiancone E

更新日期：2000-02-01 00:00:00

abstract：:Actinaria cytolysins are very potent basic toxins isolated from the venom of sea anemones, which are supposed to exert their toxic activity through formation of oligomeric pores in the host plasma membrane. To gain insight into their mechanism of action, the interaction of Stichodactyla helianthus sticholysin I (St-I)...

journal_title：Biochemistry

authors： Tejuca M,Serra MD,Ferreras M,Lanio ME,Menestrina G

更新日期：1996-11-26 00:00:00

abstract：:Uroporphyrinogen III synthase (U3S) catalyzes the asymmetrical cyclization of a linear tetrapyrrole to form the physiologically relevant uroporphyrinogen III (uro'gen III) isomer during heme biosynthesis. Here, we report four apoenzyme and one product complex crystal structures of the Thermus thermophilus (HB27) U3S p...

journal_title：Biochemistry

authors： Schubert HL,Phillips JD,Heroux A,Hill CP

更新日期：2008-08-19 00:00:00

abstract：:The initial step in the l-kynurenine pathway is oxidation of l-tryptophan to N-formylkynurenine and is catalyzed by one of two heme enzymes, tryptophan 2,3-dioxygenase (TDO) or indoleamine 2,3-dioxygenase (IDO). Here, we address the role of the conserved active site Ser167 residue in human IDO (S167A and S167H variant...

journal_title：Biochemistry

authors： Chauhan N,Basran J,Efimov I,Svistunenko DA,Seward HE,Moody PC,Raven EL

更新日期：2008-04-22 00:00:00

abstract：:Yah1p, an [Fe 2S 2]-containing ferredoxin located in the matrix of Saccharomyces cerevisiae mitochondria, functions in the synthesis of Fe/S clusters and heme a prosthetic groups. EPR, Mossbauer spectroscopy, and electron microscopy were used to characterize the Fe that accumulates in Yah1p-depleted isolated intact mi...

journal_title：Biochemistry

authors： Miao R,Martinho M,Morales JG,Kim H,Ellis EA,Lill R,Hendrich MP,Münck E,Lindahl PA

更新日期：2008-09-16 00:00:00

abstract：:Glutaminase (GLS1/2) catalyzes the conversion of L-glutamine to L-glutamate and ammonia. The level of a splice variant of GLS1 (GAC) is elevated in certain cancers, and GAC is specifically inhibited by bis-2-(5-phenylacetimido-1,2,4,thiadiazol-2-yl)ethyl sulfide (BPTES). We report here the first full-length crystal st...

journal_title：Biochemistry

authors： DeLaBarre B,Gross S,Fang C,Gao Y,Jha A,Jiang F,Song J J,Wei W,Hurov JB

更新日期：2011-12-20 00:00:00

abstract：:The circulating protein transthyretin (TTR) can unfold, oligomerize, and form highly structured amyloid fibrils that are deposited in tissues, causing organ damage and disease. This pathogenic process is caused by a heritable TTR point mutation in cases of familial TTR-related amyloidosis or wild-type TTR in cases of ...

journal_title：Biochemistry

authors： Greene MJ,Klimtchuk ES,Seldin DC,Berk JL,Connors LH

更新日期：2015-01-20 00:00:00

abstract：:Four antigenic regions of native bovine pancreatic ribonuclease have been located by using antibodies that react specifically with segments 1--13, 31--79, and 80--124. These antibodies were purified by affinity chromatography on columns to which these peptide segments were bound. Analysis of precipitin curves indicate...

journal_title：Biochemistry

authors： Chavez LG Jr,Scheraga HA

更新日期：1979-10-02 00:00:00

abstract：:The unfolded state and flexible linkers in the folded structure play essential roles in protein stability and folding and protein-protein interactions. Intrinsic to these roles is the fact that unfolded proteins and flexible linkers sample many different conformations. Polymer models may capture this and complement ex...

journal_title：Biochemistry

authors： Zhou HX

更新日期：2004-03-02 00:00:00

abstract：:The refined crystallographic structure of the "closed" conformation of chicken mitochondrial aspartate aminotransferase has been used as a template for the construction of models of the two Escherichia coli aminotransferases encoded by the tyrB and aspC genes. The main results are as follows: (1) Only minor changes ar...

journal_title：Biochemistry

authors： Seville M,Vincent MG,Hahn K

更新日期：1988-11-01 00:00:00