Influence of lipid composition on physical properties and peg-mediated fusion of curved and uncurved model membrane vesicles: "nature's own" fusogenic lipid bilayer.

abstract：:Three methods, fluorescence anisotropy of rhodamine-labeled profilin, intrinsic fluorescence and nucleotide exchange, give the same affinity, Kd = 0.1 microM, for Acanthamoeba profilins binding amoeba actin monomers with bound Mg-ATP. Replacement of serine 38 with cysteine created a unique site where labeling with rho...

journal_title：Biochemistry

authors： Vinson VK,De La Cruz EM,Higgs HN,Pollard TD

更新日期：1998-08-04 00:00:00

abstract：:We have studied the binding nature of an aromatic aldehyde to the catalytic site of liver alcohol dehydrogenase from horse (LADH) using preresonance Raman spectroscopy. The compound p-(dimethylamino)benzaldehyde (DABA) is converted to the corresponding alcohol in the presence of nicotinamide adenine dinucleotide (NADH...

journal_title：Biochemistry

authors： Callender R,Chen D,Lugtenburg J,Martin C,Rhee KW,Sloan D,Vandersteen R,Yue KT

更新日期：1988-05-17 00:00:00

abstract：:Directed hydroxyl radical probing of 16S ribosomal RNA from Fe(II) tethered to specific sites within the RNA was used to determine RNA-RNA proximities in 70S ribosomes. We have transcribed 16S ribosomal RNA in vitro as two separate fragments, covalently attached an Fe(II) probe to a 5'-guanosine-alpha-phosphorothioate...

journal_title：Biochemistry

authors： Newcomb LF,Noller HF

更新日期：1999-01-19 00:00:00

abstract：:Synthesis of a novel perylene-neomycin conjugate (3) and the properties of its binding to human telomeric G-quadruplex DNA, 5'-d[AG3(T2AG3)3] (4), are reported. Various spectroscopic techniques were employed to characterize the binding of conjugate 3 to 4. A competition dialysis assay revealed that 3 preferentially bi...

journal_title：Biochemistry

authors： Xue L,Ranjan N,Arya DP

更新日期：2011-04-12 00:00:00

abstract：:Ultraviolet irradiation of DNA results in various pyrimidine modifications. We studied the excision of an ultraviolet thymine photoproduct by Escherichia coli endonuclease III and by a preparation of human WI-38 cells. These enzymes cleave UV-irradiated DNA at apyrimidinic sites formed by glycosylic removal of the pho...

journal_title：Biochemistry

authors： Ganguly T,Weems KM,Duker NJ

更新日期：1990-08-07 00:00:00

abstract：:The slowly hydrolyzed ATP analog adenosine 5'-(gamma-thiotriphosphate) (ATP gamma S) has been used in many studies of the muscle motor protein myosin in order to form a stable "weak binding" state analogous to the actin-S1-ATP complex However, the results from studies using ATP gamma S do not always agree with the res...

journal_title：Biochemistry

authors： Resetar AM,Chalovich JM

更新日期：1995-12-12 00:00:00

abstract：:There is considerable evidence that long-range interactions stabilize residual protein structure under denaturing conditions. However, evaluation of the effect of a specific contact on structure in the denatured state has been difficult. Iso-1-cytochrome c variants with a Lys54 → His mutation form a particularly stabl...

journal_title：Biochemistry

authors： Danielson TA,Stine JM,Dar TA,Briknarova K,Bowler BE

更新日期：2017-12-26 00:00:00

abstract：:The potential of the frequently encountered (βα)8-barrel fold to acquire new functions was tested by an approach combining random mutagenesis and selection in vivo. For this purpose, the genes encoding 52 different phosphate-binding (βα)8-barrel proteins were subjected to error-prone PCR and cloned into an expression ...

journal_title：Biochemistry

authors： Rohweder B,Lehmann G,Eichner N,Polen T,Rajendran C,Ruperti F,Linde M,Treiber T,Jung O,Dettmer K,Meister G,Bott M,Gronwald W,Sterner R

更新日期：2019-10-15 00:00:00

abstract：:For the study of the protein--protein and protein--nucleic acid interactions in the assembly of virus particles, laser Raman spectra have been obtained in H2O and D2O solutions and as a function of temperature for the following Salmonella phage P22 components: mature phage particles, isolated mature phage DNA, mature ...

journal_title：Biochemistry

authors： Thomas GJ Jr,Li Y,Fuller MT,King J

更新日期：1982-08-03 00:00:00

abstract：:Recent structural studies of the HMG-CoA synthase members of the thiolase superfamily have shown that the catalytic loop containing the nucleophilic cysteine follows the phi and psi angle pattern of a II' beta turn. However, the i + 1 residue is conserved as an alanine, which is quite unusual in this position as it mu...

journal_title：Biochemistry

authors： Steussy CN,Robison AD,Tetrick AM,Knight JT,Rodwell VW,Stauffacher CV,Sutherlin AL

更新日期：2006-12-05 00:00:00

abstract：:A procedure for the preparation of coupling factor 1 (F1) from Escherichia coli lacking subunits delta and epsilon is described. Using chloroform and dimethyl sulfoxide, we can isolate F1 containing only subunits alpha, beta, and gamma [F1(alpha beta gamma)] directly from membrane vesicles in 10-mg quantities. Pure an...

journal_title：Biochemistry

authors： Tuttas-Dörschug R,Hanstein WG

更新日期：1989-06-13 00:00:00

abstract：:Guinea pig liver transglutaminase (TGase) reacts with 0.1 mM N-Cbz-L-Glu(gamma-p-nitrophenyl ester)Gly (5, prepared herein, K(M) = 0.02 mM) to undergo rapid acylation that can be followed spectrophotometrically at 400 nm (pH 7.0, 25 degrees C). Deacylation of the transiently formed thiolester acyl enzyme intermediate ...

journal_title：Biochemistry

authors： Leblanc A,Gravel C,Labelle J,Keillor JW

更新日期：2001-07-27 00:00:00

abstract：:The mutagenic and genotoxic properties of 1,N6-ethenoadenine (epsilon Ade), 3,N4-ethenocytosine (epsilon Cyt), and 4-amino-5-(imidazol-2-yl)imidazole (beta) were investigated in vivo. The former two modified bases are known DNA adducts formed by the human carcinogen vinyl chloride; beta is formed by pyrimidine ring-op...

journal_title：Biochemistry

authors： Basu AK,Wood ML,Niedernhofer LJ,Ramos LA,Essigmann JM

更新日期：1993-11-30 00:00:00

abstract：:The photosynthetic antenna of Chloroflexus aurantiacus includes bacteriochlorophyll (BChl) C740 and BChl a792, both of which occur in chlorosomes, and B808-866 (containing BChl a808 and BChl a866), which is membrane-located (subscripts refer to near-infrared absorption maxima in vivo). BChl a792 is thought to mediate ...

journal_title：Biochemistry

authors： Brune DC,King GH,Infosino A,Steiner T,Thewalt ML,Blankenship RE

更新日期：1987-12-29 00:00:00

abstract：:The Ca2+-ATPase of sarcoplasmic reticulum was purified and depleted of proteolipids by solubilization in Triton X-100 and by fractionation on a DE-52 column. The protein reconstituted by deoxycholate-cholate dialysis at low lipid to protein ratios (2-5 mg of lipid/mg of protein), with either dioleoylphosphatidylethano...

journal_title：Biochemistry

authors： Navarro J,Toivio-Kinnucan M,Racker E

更新日期：1984-01-03 00:00:00

abstract：:Molecular chaperones of the heat shock protein 70 (Hsp70) family counteract protein misfolding in a variety of neurodegenerative disease models. To determine whether human Hsp70 exerts similar effects on the aggregation of alpha-synuclein (alpha-Syn), the key component of insoluble fibrils present in Parkinson's disea...

journal_title：Biochemistry

authors： Luk KC,Mills IP,Trojanowski JQ,Lee VM

更新日期：2008-11-25 00:00:00

abstract：:Bacterial cytochrome P450cam catalyzes the hydroxylation of camphor to yield 5-exo-hydroxycamphor in vivo and in a reconstituted system with oxygen, pyridine nucleotide, flavoprotein dehydrogenase, and putidaredoxin. Product is also formed when the ferric form of the hemoprotein is mixed with the exogenous oxidants io...

journal_title：Biochemistry

authors： Gelb MH,Heimbrook DC,Mälkönen P,Sligar SG

更新日期：1982-01-19 00:00:00

abstract：:The solution structure of the dicerium(III) complex of the N-terminal domain of calmodulin (Ce2-TR1C hereafter) has been solved employing paramagnetic T1 relaxation enhancements and pseudocontact shifts introduced by the Ce3+ ions, together with conventional NOE constraints. The use of pseudocontact shift constraints ...

journal_title：Biochemistry

authors： Bentrop D,Bertini I,Cremonini MA,Forsén S,Luchinat C,Malmendal A

更新日期：1997-09-30 00:00:00

abstract：:The influenza M2 protein is the target of the amantadine family of antiviral drugs, and its transmembrane (TM) domain structure and dynamics have been extensively studied. However, little is known about the structure of the highly conserved N-terminal ectodomain, which contains epitopes targeted by influenza vaccines....

journal_title：Biochemistry

authors： Kwon B,Hong M

更新日期：2016-09-27 00:00:00

abstract：:The HIV-1 virulence factor Nef interacts with the macrophage Src-family kinase Hck, resulting in constitutive kinase activation that contributes to viral replication and immune escape. Previous chemical library screens identified the diphenylfuranopyrimdine kinase inhibitor DFP-4AB, which selectively inhibits Nef-depe...

journal_title：Biochemistry

authors： Wales TE,Hochrein JM,Morgan CR,Emert-Sedlak LA,Smithgall TE,Engen JR

更新日期：2015-10-20 00:00:00

abstract：:The thermotropic phase behavior of 10 mixed-chain phosphatidylcholines, in excess water, has been examined and compared with that of identical-chain C(16):C(16)PC by using high-resolution differential scanning calorimetry (DSC). The molecular weights (MW) of these 11 molecular species are the same, but their delta C/C...

journal_title：Biochemistry

authors： Bultmann T,Lin HN,Wang ZQ,Huang CH

更新日期：1991-07-23 00:00:00

abstract：:Salts and polyamines have a variety of effects on the physical properties of DNA, including stabilization against thermal melting. We wished to gain greater insight into the mechanism of this stabilization by ascertaining its effect on the dynamics of base opening and closing reactions, as measured by NMR. Since the b...

journal_title：Biochemistry

authors： Braunlin WH,Bloomfield VA

更新日期：1988-02-23 00:00:00

abstract：:Protein enzymes often use ionizable side chains, such as histidine, for general acid-base catalysis because the imidazole pK(a) is near neutral pH. RNA enzymes, on the other hand, are comprised of nucleotides which do not have apparent pK(a) values near neutral pH. Nevertheless, it has been recently shown that cytidin...

journal_title：Biochemistry

authors： Ravindranathan S,Butcher SE,Feigon J

更新日期：2000-12-26 00:00:00

abstract：:Green sulfur bacteria possess two light-harvesting antenna systems, the chlorosome and the Fenna-Matthews-Olson (FMO) protein. In addition to self-aggregated bacteriochlorophyll (BChl) c, chlorosomes of Chlorobium tepidum contain a small amount of BChl a (ratio 100:1). The chlorosomal BChl a is associated with CsmA, a...

journal_title：Biochemistry

authors： Pedersen MØ,Pham L,Steensgaard DB,Miller M

更新日期：2008-02-05 00:00:00

abstract：:We have previously shown that the anticodon of methionine tRNAs contains most, if not all, of the nucleotides required for specific recognition of tRNA substrates by Escherichia coli methionyl-tRNA synthetase [Schulman, L. H., & Pelka, H. (1988) Science 242, 765-768]. Previous cross-linking experiments have also ident...

journal_title：Biochemistry

authors： Ghosh G,Pelka H,Schulman LH

更新日期：1990-03-06 00:00:00

abstract：:Six affinity reagents containing chemically reactive groups, either on the phosphate residue at the 5'-end or on the 5'- or 3'-end internucleoside phosphate linkages of the oligothymidylate primers, were used to covalently modify the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). After covalent ...

journal_title：Biochemistry

authors： Andreola ML,Tarrago-Litvak L,Levina AS,Kolocheva TI,el Dirani-Diab R,Jamkovoy VI,Khalimskaya NL,Barr PJ,Litvak S,Nevinsky GA

更新日期：1993-04-13 00:00:00

abstract：:Structure-function studies of antibody-antigen systems include the identification of amino acid residues in the antigen that interact with an antibody and elucidation of their individual contributions to binding affinity. We used fluorescence correlation spectroscopy (FCS) and alanine-scanning mutagenesis to character...

journal_title：Biochemistry

authors： Tetin SY,Ruan Q,Saldana SC,Pope MR,Chen Y,Wu H,Pinkus MS,Jiang J,Richardson PL

更新日期：2006-11-28 00:00:00

abstract：:Rat alpha- and beta-parvalbumins have distinct monovalent cation-binding properties [Henzl et al. (2000) Biochemistry 39, 5859-5867]. Beta binds two Na(+) or one K(+), and alpha binds one Na(+) and no K(+). Ca(2+) abolishes these binding events, suggesting that the monovalent ions occupy the EF-hand motifs. This study...

journal_title：Biochemistry

authors： Henzl MT,Larson JD,Agah S

更新日期：2004-03-16 00:00:00

abstract：:Neuroligins 1-4 are postsynaptic transmembrane proteins capable of initiating presynaptic maturation via interactions with beta-neurexin. Both neuroligins and beta-neurexins have alternatively spliced inserts in their extracellular domains. Using analytical ultracentrifugation, we determined that the extracellular dom...

journal_title：Biochemistry

authors： Comoletti D,Flynn RE,Boucard AA,Demeler B,Schirf V,Shi J,Jennings LL,Newlin HR,Südhof TC,Taylor P

更新日期：2006-10-24 00:00:00

abstract：:Two enantiomeric analogues of farnesyl pyrophosphate (1) were tested as inhibitors and anomalous substrates of trichodiene synthase, which catalyzes the cyclization of trans,trans-farnesyl pyrophosphate (1) to the sesquiterpene hydrocarbon trichodiene (2). The reaction has been shown to involve preliminary isomerizati...

journal_title：Biochemistry

authors： Cane DE,Pawlak JL,Horak RM

更新日期：1990-06-12 00:00:00