当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Gene selection, alternative splicing, and post-translational processing regulate neuroligin selectivity for beta-neurexins.

Gene selection, alternative splicing, and post-translational processing regulate neuroligin selectivity for beta-neurexins.

Abstract：

:Neuroligins 1-4 are postsynaptic transmembrane proteins capable of initiating presynaptic maturation via interactions with beta-neurexin. Both neuroligins and beta-neurexins have alternatively spliced inserts in their extracellular domains. Using analytical ultracentrifugation, we determined that the extracellular domains of the neuroligins sediment as dimers, whereas the extracellular domains of the beta-neurexins appear monomeric. Sedimentation velocity experiments of titrated stoichiometry ratios of beta-neurexin and neuroligin suggested a 2:2 complex formation. The recognition properties of individual neuroligins toward beta-neurexin-1 (NX1beta), along with the influence of their splice inserts, were explored by surface plasmon resonance and affinity chromatography. Different neuroligins display a range of NX1beta affinities spanning more than 2 orders of magnitude. Whereas splice insert 4 in beta-neurexin appears to act only as a modulator of the neuroligin/beta-neurexin association, splice insert B in neuroligin-1 (NL1) is the key element regulating the NL1/NX1beta binding. Our data indicate that gene selection, mRNA splicing, and post-translational modifications combine to give rise to a controlled neuroligin recognition code with a rank ordering of affinities for particular neurexins that is conserved for the neuroligins across mammalian species.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Comoletti D,Flynn RE,Boucard AA,Demeler B,Schirf V,Shi J,Jennings LL,Newlin HR,Südhof TC,Taylor P

doi

10.1021/bi0614131

subject

Has Abstract

pub_date

2006-10-24 00:00:00

pages

12816-27

issue

42

eissn

0006-2960

issn

1520-4995

journal_volume

45

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Kinetic, crystallographic, and mechanistic characterization of TomN: elucidation of a function for a 4-oxalocrotonate tautomerase homologue in the tomaymycin biosynthetic pathway.

    abstract：:The biosynthesis of the C ring of the antitumor antibiotic agent, tomaymycin, is proposed to proceed through five enzyme-catalyzed steps from l-tyrosine. The genes encoding these enzymes have recently been cloned and their functions tentatively assigned, but there is limited biochemical evidence supporting the assignm...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi200947w

    authors： Burks EA,Yan W,Johnson WH Jr,Li W,Schroeder GK,Min C,Gerratana B,Zhang Y,Whitman CP

    更新日期：2011-09-06 00:00:00

  • Histone redistribution and conformational effect on chromatin induced by formaldehyde.

    abstract：:Histone redistributions between endogenous DNA in calf thymus chromatin and exogenous DNA from Clostridium perfringens (69% A + T) or from Micrococcus luteus (30% A + T) induced by 0.6 M NaCl or by 2% formaldehyde were studied by thermal denaturation. The observed redistribution occurred on histone Hl when the exogeno...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00666a002

    authors： Polacow I,Cabasso L,Li HJ

    更新日期：1976-10-19 00:00:00

  • Factors determining the orientation of axially coordinated imidazoles in heme proteins.

    abstract：:Factors determining conformations of imidazole axially coordinated to heme in heme proteins were investigated by analyzing 693 hemes in 432 different crystal structures of heme proteins from the Protein Data Bank (PDB), where at least one histidine is ligated to heme. The results from a search of the PDB for protein s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi010428q

    authors： Zarić SD,Popović DM,Knapp EW

    更新日期：2001-07-03 00:00:00

  • Purification and characterization of two new high molecular weight forms of DNA polymerase delta.

    abstract：:Two high molecular weight DNA polymerases, which we have designated delta I and delta II, have been purified from calf thymus tissue. Using Bio Rex-70, DEAE-Sephadex A-25, and DNA affinity resin chromatography followed by sucrose gradient sedimentation, we purified DNA polymerase delta I 1400-fold to a specific activi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00349a005

    authors： Crute JJ,Wahl AF,Bambara RA

    更新日期：1986-01-14 00:00:00

  • Crystal structure of ribonuclease T1 carboxymethylated at Glu58 in complex with 2'-GMP.

    abstract：:The carboxymethylation of RNase T1 at the gamma-carboxyl group of Glu58 leads to a complete loss of the enzymatic activity while it retains substrate-binding ability. Accompanying the carboxymethylation, RNase T1 undergoes a remarkable thermal stabilization of 9 degrees C in the melting temperature (Tm). In order to c...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi960493d

    authors： Ishikawa K,Suzuki E,Tanokura M,Takahashi K

    更新日期：1996-06-25 00:00:00

  • Structural effects in reactivity and adduct formation of polycyclic aromatic epoxide and diol epoxide derivatives with DNA: comparison between 1-oxiranylpyrene and benzo[a]pyrenediol epoxide.

    abstract：:Reaction of 1-oxiranylpyrene (1-OP) with DNA and the structures of the covalent and noncovalent complexes formed were studied in aqueous media (5 mM phosphate buffer with 0.1 M NaCl, pH 7) by utilizing the techniques of absorption, fluorescence and linear dichroism spectroscopy in order to gain an understanding of pos...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00318a009

    authors： Kim MH,Geacintov NE,Pope M,Harvey RG

    更新日期：1984-11-06 00:00:00

  • Characterization of a fluorescent substance P analog.

    abstract：:We describe the development and characterization of substance P labeled at Lys3 with fluorescein ([fluorescein Lys3]SP) as a fluorescent probe for the neurokinin 1 (NK1) receptor. [fluorescein Lys3]SP is an agonist at the human NK1 receptor, with an affinity for both the high-affinity and low-affinity binding states o...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00248a017

    authors： Tota MR,Daniel S,Sirotina A,Mazina KE,Fong TM,Longmore J,Strader CD

    更新日期：1994-11-08 00:00:00

  • Energy-transfer dynamics in three light-harvesting mutants of Rhodobacter sphaeroides: a picosecond spectroscopy study.

    abstract：:Picosecond absorption spectroscopy has been used to investigate energy-transfer dynamics within the LH1 and LH2 light-harvesting complexes of three mutants of Rhodobacter sphaeroides. We demonstrate that both complexes are inhomogeneous; each contains a specialized pigment pool which absorbs maximally at a longer wave...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00465a008

    authors： Hunter CN,Bergström H,van Grondelle R,Sundström V

    更新日期：1990-04-03 00:00:00

  • Structure of a Zinc Porphyrin-Substituted Bacterioferritin and Photophysical Properties of Iron Reduction.

    abstract：:The iron storage protein bacterioferritin (Bfr) binds up to 12 hemes b at specific sites in its protein shell. The heme b can be substituted with the photosensitizer Zn(II)-protoporphyrin IX (ZnPP), and photosensitized reductive iron release from the ferric oxyhydroxide {[FeO(OH)] n ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b01103

    authors： Benavides BS,Valandro S,Cioloboc D,Taylor AB,Schanze KS,Kurtz DM Jr

    更新日期：2020-04-28 00:00:00

  • Purification, characterization, and substrate and inhibitor structure-activity studies of rat liver FAD-AMP lyase (cyclizing): preference for FAD and specificity for splitting ribonucleoside diphosphate-X into ribonucleotide and a five-atom cyclic phospho

    abstract：:An enzyme with FAD-AMP lyase (cyclizing) activity, splitting FAD to AMP and riboflavin 4',5'-phosphate (cFMN), was recently identified [Fraiz, F., et al. (1998) Biochem. J. 330, 881-888]. Now, it has been purified to apparent homogeneity from a rat liver supernatant, by a procedure that includes affinity for ADP-agaro...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0157159

    authors： Cabezas A,Pinto RM,Fraiz F,Canales J,González-Santiago S,Cameselle JC

    更新日期：2001-11-13 00:00:00

  • Structural characterization of azurin from Pseudomonas aeruginosa and some of its methionine-121 mutants.

    abstract：:Azurin from Pseudomonas aeruginosa and two mutants where the methionine ligand has been mutated have been studied in order to directly investigate the functional and structural significance of this ligand in the blue copper proteins. Reduction potentials, X-ray absorption fine structure (XAFS), electron paramagnetic r...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00059a013

    authors： Murphy LM,Strange RW,Karlsson BG,Lundberg LG,Pascher T,Reinhammar B,Hasnain SS

    更新日期：1993-03-02 00:00:00

  • Cold adaptation of enzyme reaction rates.

    abstract：:A major issue for organisms living at extreme temperatures is to preserve both stability and activity of their enzymes. Cold-adapted enzymes generally have a reduced thermal stability, to counteract freezing, and show a lower enthalpy and a more negative entropy of activation compared to mesophilic and thermophilic ho...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801177k

    authors： Bjelic S,Brandsdal BO,Aqvist J

    更新日期：2008-09-23 00:00:00

  • Mechanism of flavin reduction in class 2 dihydroorotate dehydrogenases.

    abstract：:Dihydroorotate dehydrogenases (DHODs) oxidize dihydroorotate (DHO) to orotate using the FMN prosthetic group to abstract a hydride equivalent from C6 and a protein residue (Ser for Class 2 DHODs) to deprotonate C5. The fundamental question of whether the scission of the two DHO C-H bonds is concerted or stepwise was a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi060919g

    authors： Fagan RL,Nelson MN,Pagano PM,Palfey BA

    更新日期：2006-12-19 00:00:00

  • Isolation and characterization of an organic solvent soluble polypeptide component from photoreceptor complexes of Rhodospirillum rubrum.

    abstract：:An organic solvent soluble polypeptide has been isolated from photoreceptor complexes and chromatophores of Rhodospirillum rubrum. After extraction of the protein from lyophilized samples with 1:1 chloroform-methanol, it was purified by column chromatography. Its isoelectric point determined by isoelectric focusing wa...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00624a011

    authors： Tonn SJ,Gogel GE,Loach PA

    更新日期：1977-03-08 00:00:00

  • Factors affecting the stability and conformation of Locusta migratoria apolipophorin III.

    abstract：:Apolipophorin III (apoLp-III) from the migratory locust, Locusta migratoria, represents the only full-length apolipoprotein whose three-dimensional structure has been solved. In the present study, spectroscopic methods have been employed to investigate the effects of deglycosylation (via endoglycosidase F treatment) a...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00178a019

    authors： Weers PM,Kay CM,Oikawa K,Wientzek M,Van der Horst DJ,Ryan RO

    更新日期：1994-03-29 00:00:00

  • Identification of a regulatory segment of poly(ADP-ribose) glycohydrolase.

    abstract：:Coordinate regulation of PARP-1 and -2 and PARG is required for cellular responses to genotoxic stress. While PARP-1 and -2 are regulated by DNA breaks and covalent modifications, mechanisms of PARG regulation are poorly understood. We report here discovery of a PARG regulatory segment far removed linearly from residu...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi100973m

    authors： Botta D,Jacobson MK

    更新日期：2010-09-07 00:00:00

  • The conserved seven-transmembrane sequence NP(X)2,3Y of the G-protein-coupled receptor superfamily regulates multiple properties of the beta 2-adrenergic receptor.

    abstract：:The beta 2-adrenergic receptor (beta 2AR) is a member of a large superfamily of seven transmembrane domain, G-protein-coupled receptors. Within the putative seventh transmembrane domain of the beta 2AR is a sequence of amino acids, NPLIY, which is conserved with minor variations in all members of the superfamily. Prev...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00047a003

    authors： Barak LS,Ménard L,Ferguson SS,Colapietro AM,Caron MG

    更新日期：1995-11-28 00:00:00

  • Conservation of a helix-stabilizing dipole moment in the PP-fold family of regulatory peptides.

    abstract：:Investigation of the charge distribution for all known members of the PP-fold family of peptides reveals a common pattern characterized by a cluster of negative charges in the beta-turn region and a cluster of positive charges in the receptor-binding region of the peptide. Detailed analysis of the electrostatic proper...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00063a005

    authors： Bjørnholm B,Jørgensen FS,Schwartz TW

    更新日期：1993-03-30 00:00:00

  • Ginsenosides are potent and selective inhibitors of some calmodulin-dependent phosphodiesterase isozymes.

    abstract：:The effects of various ginsenosides on calmodulin-dependent phosphodiesterase isozymes have been investigated. Ginsenosides were found to be potent inhibitors of bovine heart calmodulin-dependent phosphodiesterase and the 60-kDa isozyme of bovine brain calmodulin-dependent phosphodiesterase but not of the 63-kDa isozy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00070a001

    authors： Sharma RK,Kalra J

    更新日期：1993-05-18 00:00:00

  • DNA degradation by bleomycin: evidence for 2'R-proton abstraction and for C-O bond cleavage accompanying base propenal formation.

    abstract：:Reaction of poly(dA-[2'S-3H]dU) with activated bleomycin yields [3H]uracil propenal that completely retains the tritium label. In contrast, we have previously shown that reaction of poly(dA-[2'R-3H]dU) with activated bleomycin affords unlabeled uracil propenal [Wu, J. C., Kozarich, J. W., & Stubbe, J. (1983) J. Biol. ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00369a037

    authors： Ajmera S,Wu JC,Worth L Jr,Rabow LE,Stubbe J,Kozarich JW

    更新日期：1986-10-21 00:00:00

  • Proximity of thiol esters and bait region in human alpha 2-macroglobulin: paramagnetic mapping.

    abstract：:The two key structural features of alpha 2-macroglobulin (alpha 2M) involved in inhibitory caging of proteases are the thiol ester and the bait region. This paper examines the environment of the hydrolyzed thiol ester in methylamine-treated human alpha 2M and the separation between the bait region and the thiol ester ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00408a036

    authors： Gettins P,Beth AH,Cunningham LW

    更新日期：1988-04-19 00:00:00

  • The family 1 beta-glucosidases from Pyrococcus furiosus and Agrobacterium faecalis share a common catalytic mechanism.

    abstract：:Comparisons of catalytic mechanisms have not previously been performed for homologous enzymes from hyperthermophilic and mesophilic sources. Here, the beta-glucosidase from the hyperthermophilic archaeon Pyrococcus furiosus was recombinantly produced in Escherichia coli and shown to have biophyscial and biochemical pr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9814944

    authors： Bauer MW,Kelly RM

    更新日期：1998-12-08 00:00:00

  • DNA polymerase λ inactivation by oxidized abasic sites.

    abstract：:Base excision repair (BER) plays a vital role in maintaining genomic integrity in mammalian cells. DNA polymerase λ (Pol λ) is believed to play a backup role to DNA polymerase β (Pol β) in base excision repair. Two oxidized abasic lesions that are produced by a variety of DNA-damaging agents, including several antitum...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi301592x

    authors： Stevens AJ,Guan L,Bebenek K,Kunkel TA,Greenberg MM

    更新日期：2013-02-05 00:00:00

  • Metal-induced changes in the fluorescence properties of tyrosine and tryptophan site-specific mutants of oncomodulin.

    abstract：:Oncomodulin is a 108-residue, oncodevelopmental protein containing two calcium-binding sites identified as the CD- and EF-loops. The protein contains no tryptophan and only two tyrosine residues, one which is a calcium ligand in the CD-loop (Tyr-57) and one which lies in the flanking D-helix of this loop (Tyr-65). Sit...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00244a038

    authors： Hutnik CM,MacManus JP,Banville D,Szabo AG

    更新日期：1991-07-30 00:00:00

  • Biochemical and Functional Evaluation of the Intramolecular Disulfide Bonds in the Zinc-Chelating Antimicrobial Protein Human S100A7 (Psoriasin).

    abstract：:Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00781

    authors： Cunden LS,Brophy MB,Rodriguez GE,Flaxman HA,Nolan EM

    更新日期：2017-10-31 00:00:00

  • Heme synthase (ferrochelatase) catalyzes the removal of iron from heme and demetalation of metalloporphyrins.

    abstract：:The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Ra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi701460x

    authors： Taketani S,Ishigaki M,Mizutani A,Uebayashi M,Numata M,Ohgari Y,Kitajima S

    更新日期：2007-12-25 00:00:00

  • Altering the RNase H primer grip of human immunodeficiency virus reverse transcriptase modifies cleavage specificity.

    abstract：:Recent crystallographic data suggest that conserved residues in the connection subdomain and C-terminal ribonuclease H (RNase H) domain of human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT) contact the nascent DNA primer and modulate the trajectory of the template relative to the RNase H catalytic ce...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015970t

    authors： Rausch JW,Lener D,Miller JT,Julias JG,Hughes SH,Le Grice SF

    更新日期：2002-04-16 00:00:00

  • New insights into inhibitor design from the crystal structure and NMR studies of Escherichia coli GAR transformylase in complex with beta-GAR and 10-formyl-5,8,10-trideazafolic acid.

    abstract：:The crystal structure of Escherichia coli GAR Tfase at 2.1 A resolution in complex with 10-formyl-5,8,10-trideazafolic acid (10-formyl-TDAF, K(i) = 260 nM), an inhibitor designed to form an enzyme-assembled multisubstrate adduct with the substrate, beta-GAR, was studied to determine the exact nature of its inhibitory ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991888a

    authors： Greasley SE,Yamashita MM,Cai H,Benkovic SJ,Boger DL,Wilson IA

    更新日期：1999-12-21 00:00:00

  • Conversion of a group II intron into a new multiple-turnover ribozyme that selectively cleaves oligonucleotides: elucidation of reaction mechanism and structure/function relationships.

    abstract：:The self-splicing ai5g group II intron was transformed into a three-part ribozyme that site-specifically cleaves small oligonucleotide substrates with multiple turnover. The ribozyme is composed of intron domain 1 (D1, 425 nucleotides), with catalytically essential domain 5 (D5, 58 nucleotides) provided separately as ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00009a028

    authors： Michels WJ Jr,Pyle AM

    更新日期：1995-03-07 00:00:00

  • Active-site cobalt(II)-substituted horse liver alcohol dehydrogenase: characterization of intermediates in the oxidation and reduction processes as a function of pH.

    abstract：:Substitution of Co(II) for the catalytic site Zn(II) of horse liver alcohol dehydrogenase (LADH) yields an active enzyme derivative, CoIIE, with characteristic Co(II) charge-transfer and d-d electronic transitions that are sensitive to the events which take place during catalysis [Koerber, S. C., MacGibbon, A. K. H., ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00377a031

    authors： Sartorius C,Gerber M,Zeppezauer M,Dunn MF

    更新日期：1987-02-10 00:00:00